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The map label for this gene is miaA

Identifier: 110679815

GI number: 110679815

Start: 2468851

End: 2469690

Strand: Reverse

Name: miaA

Synonym: RD1_2584

Alternate gene names: 110679815

Gene position: 2469690-2468851 (Counterclockwise)

Preceding gene: 110679829

Following gene: 110679813

Centisome position: 59.75

GC content: 64.52

Gene sequence:

>840_bases
GTGCCGGAGAAACCGGTGTTGATTGCCGGGCCAACAGCGTCCGGCAAATCCGCGCTGGCCTTGCGGATTGCCGCCGAACA
GGGCGGGGTGATCGTGAATGCCGACGCCAGTCAGGGGTATGATTGCTGGCGGGTGTTGACCGCCCGCCCCGCGCCGGACG
AAGAACGGCAAACGCCACACGCGCTCTATGGCCATCTGAATGCGAAGGTGCGCTATTCCGCCGGGCATTGGTTGCGCGAT
GTGATGCCGTATTTGGCTGGCGAAGCGCGCCCGATCATTGTCGGGGGCACAGGGTTGTACTTCACTGCCCTGACGCGGGG
CCTTGCAGATATCCCGCCCACACCGGCAGAAATCCGCGCAAAGGGGGATCGTCTGGAACGGGCCGAGATGCTGGCAGCGC
TGGACGCCCGGACCCGCGACAGAATCGACAACGCCAACCGCGCGCGGGTGCAACGTGCCTGGGAGGTCCAAATGGCAACC
GGGCGCGGGCTGGCGGCATGGCAGGATGAAACCGGGGCGCCTGCCCTGCCCCTGTCAGCGGCGCAGGCAATCGTTTTCGA
CGTGGACAAAGAAGTGCTGAACGCGCGCATCGAAAAGCGATTTGACCTGATGCTGGCGGCAGGTGCCCTGGAAGAGGCCC
GCCGCGTCCTGCCGGACTATGACCCCACCCTGCCCGCGCATCGGGCCATCGGCGCACCGGAGTTGATCGCCCATCTCAAA
GGTCAGATTTCGCTGGAAGCCGCGCGCGAGGCCGCCATCACCGCAACACGTCAATATGCCAAACGGCAACGCACGTGGTT
TCGCTCAAAAATGCGGCATTGGTCCGCTTACATGCCTTGA

Upstream 100 bases:

>100_bases
TCCCAAAACCGCGGGAACTGCAATGCACCGAACTTTCAAGGGCGCGTAAAAAATGGACGAACGCCTCGTATTGAACCATC
AGACGGTGATGGGAATGCTG

Downstream 100 bases:

>100_bases
AATCGGCCACAGCACTTTCAAAAAGAGAACTTTCATGCCGCTGATTGGCGAAATTCGGGGCGTTCATTTGACACTATTCA
ACTTTTGCGAGAAAATCAGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MPEKPVLIAGPTASGKSALALRIAAEQGGVIVNADASQGYDCWRVLTARPAPDEERQTPHALYGHLNAKVRYSAGHWLRD
VMPYLAGEARPIIVGGTGLYFTALTRGLADIPPTPAEIRAKGDRLERAEMLAALDARTRDRIDNANRARVQRAWEVQMAT
GRGLAAWQDETGAPALPLSAAQAIVFDVDKEVLNARIEKRFDLMLAAGALEEARRVLPDYDPTLPAHRAIGAPELIAHLK
GQISLEAAREAAITATRQYAKRQRTWFRSKMRHWSAYMP

Sequences:

>Translated_279_residues
MPEKPVLIAGPTASGKSALALRIAAEQGGVIVNADASQGYDCWRVLTARPAPDEERQTPHALYGHLNAKVRYSAGHWLRD
VMPYLAGEARPIIVGGTGLYFTALTRGLADIPPTPAEIRAKGDRLERAEMLAALDARTRDRIDNANRARVQRAWEVQMAT
GRGLAAWQDETGAPALPLSAAQAIVFDVDKEVLNARIEKRFDLMLAAGALEEARRVLPDYDPTLPAHRAIGAPELIAHLK
GQISLEAAREAAITATRQYAKRQRTWFRSKMRHWSAYMP
>Mature_278_residues
PEKPVLIAGPTASGKSALALRIAAEQGGVIVNADASQGYDCWRVLTARPAPDEERQTPHALYGHLNAKVRYSAGHWLRDV
MPYLAGEARPIIVGGTGLYFTALTRGLADIPPTPAEIRAKGDRLERAEMLAALDARTRDRIDNANRARVQRAWEVQMATG
RGLAAWQDETGAPALPLSAAQAIVFDVDKEVLNARIEKRFDLMLAAGALEEARRVLPDYDPTLPAHRAIGAPELIAHLKG
QISLEAAREAAITATRQYAKRQRTWFRSKMRHWSAYMP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=26.6881028938907, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1790613, Length=286, Percent_Identity=33.2167832167832, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=27.027027027027, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24649830, Length=235, Percent_Identity=30.6382978723404, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ROSDO (Q166F7)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682822.1
- ProteinModelPortal:   Q166F7
- SMR:   Q166F7
- STRING:   Q166F7
- GeneID:   4195024
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2584
- NMPDR:   fig|375451.6.peg.2401
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   RDEN375451:RD1_2584-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 30643; Mature: 30512

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKPVLIAGPTASGKSALALRIAAEQGGVIVNADASQGYDCWRVLTARPAPDEERQTPH
CCCCCEEEECCCCCCCCEEEEEEEECCCCEEEECCCCCCCCCEEEEECCCCCCHHCCCCH
ALYGHLNAKVRYSAGHWLRDVMPYLAGEARPIIVGGTGLYFTALTRGLADIPPTPAEIRA
HHHHCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHC
KGDRLERAEMLAALDARTRDRIDNANRARVQRAWEVQMATGRGLAAWQDETGAPALPLSA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCEECCCCCCCCCCCHHH
AQAIVFDVDKEVLNARIEKRFDLMLAAGALEEARRVLPDYDPTLPAHRAIGAPELIAHLK
HHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHC
GQISLEAAREAAITATRQYAKRQRTWFRSKMRHWSAYMP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PEKPVLIAGPTASGKSALALRIAAEQGGVIVNADASQGYDCWRVLTARPAPDEERQTPH
CCCCEEEECCCCCCCCEEEEEEEECCCCEEEECCCCCCCCCEEEEECCCCCCHHCCCCH
ALYGHLNAKVRYSAGHWLRDVMPYLAGEARPIIVGGTGLYFTALTRGLADIPPTPAEIRA
HHHHCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHC
KGDRLERAEMLAALDARTRDRIDNANRARVQRAWEVQMATGRGLAAWQDETGAPALPLSA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCEECCCCCCCCCCCHHH
AQAIVFDVDKEVLNARIEKRFDLMLAAGALEEARRVLPDYDPTLPAHRAIGAPELIAHLK
HHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHC
GQISLEAAREAAITATRQYAKRQRTWFRSKMRHWSAYMP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA