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The map label for this gene is pleD [H]

Identifier: 110679764

GI number: 110679764

Start: 2414021

End: 2415490

Strand: Reverse

Name: pleD [H]

Synonym: RD1_2527

Alternate gene names: 110679764

Gene position: 2415490-2414021 (Counterclockwise)

Preceding gene: 110679770

Following gene: 110679763

Centisome position: 58.44

GC content: 60.68

Gene sequence:

>1470_bases
TTGTTAACCAATCTGCGATCAATGTCAGCCACCCGAACCTCCCTTCGAAAGGACGCAATCAGCGTGCAGGGCAAAATTCT
CATAGTAGATGCGATATCCACTAATCGCATTGTCTTAAAGGTAAAACTTGCGTCCGCTTTCTATGAAGTTCTGCAAGCAT
CCACTGCGGATGAGGCTTGCGTCGCCGCTCTGAGGCACGCACCCGACCTCATCATCTCAGCCATGGCTTTACCCGACGGC
GATGCGGCAGGCCTGTGTGGCAGGTTGCACCGAAATCCCCAGACGCGGTCTATTCCCATGATGGTCGTGGCGTGCCGACC
CGACACACAAAGCCGCCTCGCCGCGCTGGAGGCAGGCGTTCAGGATGTCATGGTCAAACCCATTGACGACACCTTGCTGT
TGGCTCGGGTGCGCAGCCTGATCCGGGCGCACAATACGGCTGCCGAGTGGCAGATGCGCGACGACACCTCCAGAGCCCTC
GGTTTTGCCGAAGATACGGCCGATTTCGGCCCGGCAGGACGCACTGTCATGGTGTGCTCCGGGGTAAAAACGGCACAGAA
CTGGATCCAGCAGCTGCGCCCGCACCTCAGGTCTTCCCTGACGCAGGCCTCGCCTGAAACGGCTTTGCGCGATCTGACAC
CCGGGCGCATTCCCGATGTCTTTATCATGGTGCTCGATGATGGCGACAAAATCAGCGGAGAGATCCTGCGCTTGCTGGCA
GCGGTGCGTGCAACCGCGATCACCCGCCATGCCGGCATTCTGGTGATCCAGCCGCGTCCCGACCCCGGGCTTGGGGCATA
TGCGCTCGACCTTGGGGCCGACGATCTGATGACCGACGGCTTCAACAAGGACGAATTGGTGTTGCGTATCAAGGCGTTGA
TGCGGCGCAAGCGTATTGCGGACCAACTGCGCGCAACTGTGCGCACCGGTCTGAAGGCCGCGGTGTCTGACCCGCTCACG
GGGCTTCACAACCGCCGATATGCCCTGCCCCACCTGTCACGGGTTGCCGAACATTCAGCAGCCACCGGGCGCAGCTTTGC
GGTCATGGTCGCCGATCTTGATCATTTCAAACAGATCAATGACGTTTACGGGCATGCCTCCGGCGATGCTGTGCTGGTGG
AAGCAGCGCGGCGCTTGCGGGAAAACCTGCGCGGTGTCGATATGCTGGCGCGCATCGGCGGCGAAGAATTCCTGATCGTC
ATGCCCGCGACGCCCTTCAGCGAGGCGCGCGTCTCTGCCAGCAGGCTGTGCCGAAAAATAGGCGGGCAAGGCTTTAAGAT
ACCGGGCTCCAATGAGCTTGTGCAGGTCACGATTTCCATCGGTGTCACGATGGGTGGCGTCAGGAGCCTGCGCGGCGTGC
AACCACCGCCACTGGATGCAAACGAACTTCTGGATGCCGCGGACAAGGCGCTTTACGAGGCCAAGCTGCAGGGGCGAAAT
CAGGTCAACCTTGCCCGCCCTGCCGCCTGA

Upstream 100 bases:

>100_bases
TCACCTGCCAGCCATGCCAAAACCTGCAGCGCAACCACTTCGGCGGACTCTTGCGTATGGGACATGCTACACCTCAAATT
TTAAATCTCTTTGAAACGGT

Downstream 100 bases:

>100_bases
TCCCTCTTAAGGATAAGGCAGCATCGGATTGCCCGGGCCGTGACACGCTCAAGCGGCGGCAGGGTTTTCCGCGTCCACAA
AGAACCAAATACCCCGCCTT

Product: diguanylate cyclase response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MLTNLRSMSATRTSLRKDAISVQGKILIVDAISTNRIVLKVKLASAFYEVLQASTADEACVAALRHAPDLIISAMALPDG
DAAGLCGRLHRNPQTRSIPMMVVACRPDTQSRLAALEAGVQDVMVKPIDDTLLLARVRSLIRAHNTAAEWQMRDDTSRAL
GFAEDTADFGPAGRTVMVCSGVKTAQNWIQQLRPHLRSSLTQASPETALRDLTPGRIPDVFIMVLDDGDKISGEILRLLA
AVRATAITRHAGILVIQPRPDPGLGAYALDLGADDLMTDGFNKDELVLRIKALMRRKRIADQLRATVRTGLKAAVSDPLT
GLHNRRYALPHLSRVAEHSAATGRSFAVMVADLDHFKQINDVYGHASGDAVLVEAARRLRENLRGVDMLARIGGEEFLIV
MPATPFSEARVSASRLCRKIGGQGFKIPGSNELVQVTISIGVTMGGVRSLRGVQPPPLDANELLDAADKALYEAKLQGRN
QVNLARPAA

Sequences:

>Translated_489_residues
MLTNLRSMSATRTSLRKDAISVQGKILIVDAISTNRIVLKVKLASAFYEVLQASTADEACVAALRHAPDLIISAMALPDG
DAAGLCGRLHRNPQTRSIPMMVVACRPDTQSRLAALEAGVQDVMVKPIDDTLLLARVRSLIRAHNTAAEWQMRDDTSRAL
GFAEDTADFGPAGRTVMVCSGVKTAQNWIQQLRPHLRSSLTQASPETALRDLTPGRIPDVFIMVLDDGDKISGEILRLLA
AVRATAITRHAGILVIQPRPDPGLGAYALDLGADDLMTDGFNKDELVLRIKALMRRKRIADQLRATVRTGLKAAVSDPLT
GLHNRRYALPHLSRVAEHSAATGRSFAVMVADLDHFKQINDVYGHASGDAVLVEAARRLRENLRGVDMLARIGGEEFLIV
MPATPFSEARVSASRLCRKIGGQGFKIPGSNELVQVTISIGVTMGGVRSLRGVQPPPLDANELLDAADKALYEAKLQGRN
QVNLARPAA
>Mature_489_residues
MLTNLRSMSATRTSLRKDAISVQGKILIVDAISTNRIVLKVKLASAFYEVLQASTADEACVAALRHAPDLIISAMALPDG
DAAGLCGRLHRNPQTRSIPMMVVACRPDTQSRLAALEAGVQDVMVKPIDDTLLLARVRSLIRAHNTAAEWQMRDDTSRAL
GFAEDTADFGPAGRTVMVCSGVKTAQNWIQQLRPHLRSSLTQASPETALRDLTPGRIPDVFIMVLDDGDKISGEILRLLA
AVRATAITRHAGILVIQPRPDPGLGAYALDLGADDLMTDGFNKDELVLRIKALMRRKRIADQLRATVRTGLKAAVSDPLT
GLHNRRYALPHLSRVAEHSAATGRSFAVMVADLDHFKQINDVYGHASGDAVLVEAARRLRENLRGVDMLARIGGEEFLIV
MPATPFSEARVSASRLCRKIGGQGFKIPGSNELVQVTISIGVTMGGVRSLRGVQPPPLDANELLDAADKALYEAKLQGRN
QVNLARPAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=186, Percent_Identity=33.8709677419355, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI145693134, Length=207, Percent_Identity=32.3671497584541, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=34.3195266272189, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=202, Percent_Identity=35.1485148514851, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=34.9112426035503, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081977, Length=200, Percent_Identity=30.5, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=32.6086956521739, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787816, Length=179, Percent_Identity=33.5195530726257, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788085, Length=178, Percent_Identity=32.5842696629214, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787802, Length=139, Percent_Identity=34.5323741007194, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788956, Length=182, Percent_Identity=35.1648351648352, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787541, Length=275, Percent_Identity=26.1818181818182, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=92, Percent_Identity=35.8695652173913, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52816; Mature: 52816

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNLRSMSATRTSLRKDAISVQGKILIVDAISTNRIVLKVKLASAFYEVLQASTADEAC
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEHHHHHHHHHHCCCCHHHH
VAALRHAPDLIISAMALPDGDAAGLCGRLHRNPQTRSIPMMVVACRPDTQSRLAALEAGV
HHHHHHCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCH
QDVMVKPIDDTLLLARVRSLIRAHNTAAEWQMRDDTSRALGFAEDTADFGPAGRTVMVCS
HHHEECCCCHHHHHHHHHHHHHHCCCHHCEEECCCHHHHCCCCHHCCCCCCCCCEEEEEC
GVKTAQNWIQQLRPHLRSSLTQASPETALRDLTPGRIPDVFIMVLDDGDKISGEILRLLA
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
AVRATAITRHAGILVIQPRPDPGLGAYALDLGADDLMTDGFNKDELVLRIKALMRRKRIA
HHHHHHHHHHCCEEEEECCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
DQLRATVRTGLKAAVSDPLTGLHNRRYALPHLSRVAEHSAATGRSFAVMVADLDHFKQIN
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
DVYGHASGDAVLVEAARRLRENLRGVDMLARIGGEEFLIVMPATPFSEARVSASRLCRKI
HHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
GGQGFKIPGSNELVQVTISIGVTMGGVRSLRGVQPPPLDANELLDAADKALYEAKLQGRN
CCCCEECCCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
QVNLARPAA
CCCCCCCCC
>Mature Secondary Structure
MLTNLRSMSATRTSLRKDAISVQGKILIVDAISTNRIVLKVKLASAFYEVLQASTADEAC
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEHHHHHHHHHHCCCCHHHH
VAALRHAPDLIISAMALPDGDAAGLCGRLHRNPQTRSIPMMVVACRPDTQSRLAALEAGV
HHHHHHCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCH
QDVMVKPIDDTLLLARVRSLIRAHNTAAEWQMRDDTSRALGFAEDTADFGPAGRTVMVCS
HHHEECCCCHHHHHHHHHHHHHHCCCHHCEEECCCHHHHCCCCHHCCCCCCCCCEEEEEC
GVKTAQNWIQQLRPHLRSSLTQASPETALRDLTPGRIPDVFIMVLDDGDKISGEILRLLA
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
AVRATAITRHAGILVIQPRPDPGLGAYALDLGADDLMTDGFNKDELVLRIKALMRRKRIA
HHHHHHHHHHCCEEEEECCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
DQLRATVRTGLKAAVSDPLTGLHNRRYALPHLSRVAEHSAATGRSFAVMVADLDHFKQIN
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
DVYGHASGDAVLVEAARRLRENLRGVDMLARIGGEEFLIVMPATPFSEARVSASRLCRKI
HHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
GGQGFKIPGSNELVQVTISIGVTMGGVRSLRGVQPPPLDANELLDAADKALYEAKLQGRN
CCCCEECCCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
QVNLARPAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA