| Definition | Roseobacter denitrificans OCh 114, complete genome. |
|---|---|
| Accession | NC_008209 |
| Length | 4,133,097 |
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The map label for this gene is helD [H]
Identifier: 110679728
GI number: 110679728
Start: 2369250
End: 2372000
Strand: Reverse
Name: helD [H]
Synonym: RD1_2487
Alternate gene names: 110679728
Gene position: 2372000-2369250 (Counterclockwise)
Preceding gene: 110679729
Following gene: 110679727
Centisome position: 57.39
GC content: 57.83
Gene sequence:
>2751_bases GTGTTTTCTTCGTCTTTCGCCCTCAATCTCCATGGCGACAAACAGGTTGTTCTCCCAGCCGTCAACAAAGCAGCGGCGAA TGCTTTCGCCCAAGCTTTTGGGCAGGCGTGGTCCAAATTCAACCTTAGGGAACTTGCCAAGGAAGAGGAGAGAATCCAGC GGCTCCTTCAATTCCTAGAGAAGCTCCAAGCGCCTGAGCGATACCCGTCAGCATTCCATATCGACCCATTGGCACGAGAG GCATTGAACCTGACTGACCGTGTCTTGTCCAAGCTCAACGCCGAGGCTGTTGGCGACAAGACGATGCGTCGGATCGCCCC GATCCTTTCCTTCTCTGCTGACCCTGCCAAAGCCCGCGAAGCCGCGATCGAGCGCTTTGTCGATGCACAGCTCGACCGTT GGACGGAATTTCTCGACACCGTCGAGTCCATGCCGTTGACGCCCGAACAACGTCTGTCCGTAGTTGTCGATGAGGATGCG ACGCTGGTTCTAGCCGGGGCCGGATCGGGCAAGACCAGCGTCATCACCGCGAAAGCGGCCTATCTCGTTAAGGCCGGGAT CAGGTCTCCGGAAGAACTGCTTCTCCTGGCCTTTGCACGAGATGCAGCGACTGAGATGTCTGAGAGGATCGAAGCTCGTT GTGGCGTGCCCATCGCCGCGCGCACGTTCCATGCGCTGGCCTACGAGATCATAGGCGATGTCGAAGGGGAAAAGCCCGCG CTTGCGCCGACGGCAACGGACGACAAGGCGTTCCTATCGCTTATCCGGGACATCCTGCGATACATCGTCAGCCGCGTCAG CGACGTGGCAGAAACCGTGATCGGCTGGTTTGCGGGCTTCTTCGACGACTTCCCATCACCTTGGGACTTCAAGTCTGCCC ATGAATGGTATTCCGAGGTCGAAAGCCGCAATTTACGGACGCTGCAAGGGGAAACCGTCAACAGTTTCGAAGAACTAATC ATCGCGAACTGGTTGTATCGGAACGGCATTGCCTTCGAATACGAGCCTACCTACGAACACAAGCTGACCGGGACTGGGCG ACGTGTCTACACCCCGGATTTCCGTTTGACCGAAAGTGGCGTCTACATCGAACACTTCGGGGTCCGGAAGAGACGAACCT CAGATGGCACGGAGCAGCTGACAACCGCGCCGTTTGTCGATCGAGACGAATACCTGGCCAGCATGGACTGGAAGCGAAAG GTCCATACGGAACATGAGACGGTCCTGATCGAAACCTATAGCTGGGAGCGCGAGGAGGGTCGGTTGCTCGACGCGTTAGC CGAAAAGCTCGCGCCACACGTCACCTTGAGGCCGATCCCGGATATCGAGATTTATGATCAGGTCACACAGGTCGGTGTCG TGGACGGGTTCTCGTCGCTGATCGGCACCTTTCTTCGCCATTTCAAGAACGGCGGGTATCAGATCGATGACTGCTCCGAC AAAGCATCCACCCTGAAAATGGGAAAGAGAGCCGATGCTTTCCTCAAGATCTTCGGAGCGGTCTTTCGAGAGTATCAGAA CCGCCTAGGCGACCGTATCGATTTCGAGGACATGGTGATCCGGGCGACTGCGCATGTCGAAAGAGGACGTTACAACAGCC CGTTTCGCCACATTCTCGTTGACGAGTTTCAGGACATCTCGACCGGAAGAGCGCGTCTTATCAAAGCACTCAAGGAGCAG CACGCGGATGCGAAGGTCTTTGCGGTTGGGGATGATTGGCAATCGATCTACCGCTTTGTCGGCTCCGATATCCATATCAT GCGAAACTTCGGGGCCGAGTTTGGGGGGCAGTTTGCCGGCATAACCGGCATCCACCGAACCGTTGATCTTGGTCGCACTT TCCGATCAGTTGACAAGATCGCGCTCGCATCACGACGCTTCGTCTTGCGCAACCCGGCCCAGATCACAAAGACAGTGCGC GCAGCAGGAGAAACGGACCAGCCTGCGATTCAGGTTGCATGGACCCGGCGCGACAACGCCGAAAACGTCCTCCACGAAAC CCTCGCTTCTCTGACAGAGGCGAGCGCAGATGGCGTCCAAAAGCCGTCCGTGCTCCTACTTGGCCGATACAGGTTCAATG AACCTGACCTCAGGCGCCTGCGGCGGCAACACCCGGCACTTTCGCTGAGTTTCAAGACGATCCATGCTTCCAAGGGCCTG GAGGCAGACCATGTGGTCATTCTCGGGGCCGACAAGGCTCGAATGGGGTTCCCGTCCATGATCGTTGACGATCCGTTGCT AAGCCTCGTCTCACCGGAAGCGGAGCCGTTCGAGAACGCAGAAGAGCGTCGCGTGATGTACGTTGCTATGACGCGCGCCC GACGTTCGGTGACAATCCTTGCGTCGGAAGCGCGGCCTTCGATCTTCGTTACCGAACTGATGAACGATCCGGATTACGGC GTCGACAGCCCGGTCGAGGCGACGGAACGCACACACACCTGCCTGCAATGCGGGGGGCGTCTTCTCTACATGCCCGGGCA GGATGGACCGGGATGGTATCGCTGCGAGCATGTGAAATTGTGCGGAAACCGTATGCCGGCATGTCCAGAATGCGGCGTAG GGCTGCCGGTCCGGGACCCAAAACGCAGTCTGATGGAATGTTCGGAATGCGATACGTCGCAGCAAGCCTGCCCCAGCTGC GACGCTGGATGGCTCATTGAACGCCGTGGCAGGTTCGGACCCTTCCTCGGGTGTGTCCGTTTCCCGGACTGCAATGGGAA AGCGAAGATCGCAAAGACTGCATCGCGCTAA
Upstream 100 bases:
>100_bases AAACATGGGGATCGGCGGAGTGACACCGATACAGAAACTGAAAGCAGCATAGATTCTACTGACGCGCCCCTCTGAAAATG GGGGGATTACCCAGGTAGGA
Downstream 100 bases:
>100_bases ATACACTATCAAGACCCATCACGTCGGGCGCAAGCACCCAAAGTTGCGCAACTAAGTGACATAAGTCTGACCTTCCCCTA GCCTGGCATGATGTCGAGCC
Product: helicase IV
Products: NA
Alternate protein names: 75 kDa helicase [H]
Number of amino acids: Translated: 916; Mature: 916
Protein sequence:
>916_residues MFSSSFALNLHGDKQVVLPAVNKAAANAFAQAFGQAWSKFNLRELAKEEERIQRLLQFLEKLQAPERYPSAFHIDPLARE ALNLTDRVLSKLNAEAVGDKTMRRIAPILSFSADPAKAREAAIERFVDAQLDRWTEFLDTVESMPLTPEQRLSVVVDEDA TLVLAGAGSGKTSVITAKAAYLVKAGIRSPEELLLLAFARDAATEMSERIEARCGVPIAARTFHALAYEIIGDVEGEKPA LAPTATDDKAFLSLIRDILRYIVSRVSDVAETVIGWFAGFFDDFPSPWDFKSAHEWYSEVESRNLRTLQGETVNSFEELI IANWLYRNGIAFEYEPTYEHKLTGTGRRVYTPDFRLTESGVYIEHFGVRKRRTSDGTEQLTTAPFVDRDEYLASMDWKRK VHTEHETVLIETYSWEREEGRLLDALAEKLAPHVTLRPIPDIEIYDQVTQVGVVDGFSSLIGTFLRHFKNGGYQIDDCSD KASTLKMGKRADAFLKIFGAVFREYQNRLGDRIDFEDMVIRATAHVERGRYNSPFRHILVDEFQDISTGRARLIKALKEQ HADAKVFAVGDDWQSIYRFVGSDIHIMRNFGAEFGGQFAGITGIHRTVDLGRTFRSVDKIALASRRFVLRNPAQITKTVR AAGETDQPAIQVAWTRRDNAENVLHETLASLTEASADGVQKPSVLLLGRYRFNEPDLRRLRRQHPALSLSFKTIHASKGL EADHVVILGADKARMGFPSMIVDDPLLSLVSPEAEPFENAEERRVMYVAMTRARRSVTILASEARPSIFVTELMNDPDYG VDSPVEATERTHTCLQCGGRLLYMPGQDGPGWYRCEHVKLCGNRMPACPECGVGLPVRDPKRSLMECSECDTSQQACPSC DAGWLIERRGRFGPFLGCVRFPDCNGKAKIAKTASR
Sequences:
>Translated_916_residues MFSSSFALNLHGDKQVVLPAVNKAAANAFAQAFGQAWSKFNLRELAKEEERIQRLLQFLEKLQAPERYPSAFHIDPLARE ALNLTDRVLSKLNAEAVGDKTMRRIAPILSFSADPAKAREAAIERFVDAQLDRWTEFLDTVESMPLTPEQRLSVVVDEDA TLVLAGAGSGKTSVITAKAAYLVKAGIRSPEELLLLAFARDAATEMSERIEARCGVPIAARTFHALAYEIIGDVEGEKPA LAPTATDDKAFLSLIRDILRYIVSRVSDVAETVIGWFAGFFDDFPSPWDFKSAHEWYSEVESRNLRTLQGETVNSFEELI IANWLYRNGIAFEYEPTYEHKLTGTGRRVYTPDFRLTESGVYIEHFGVRKRRTSDGTEQLTTAPFVDRDEYLASMDWKRK VHTEHETVLIETYSWEREEGRLLDALAEKLAPHVTLRPIPDIEIYDQVTQVGVVDGFSSLIGTFLRHFKNGGYQIDDCSD KASTLKMGKRADAFLKIFGAVFREYQNRLGDRIDFEDMVIRATAHVERGRYNSPFRHILVDEFQDISTGRARLIKALKEQ HADAKVFAVGDDWQSIYRFVGSDIHIMRNFGAEFGGQFAGITGIHRTVDLGRTFRSVDKIALASRRFVLRNPAQITKTVR AAGETDQPAIQVAWTRRDNAENVLHETLASLTEASADGVQKPSVLLLGRYRFNEPDLRRLRRQHPALSLSFKTIHASKGL EADHVVILGADKARMGFPSMIVDDPLLSLVSPEAEPFENAEERRVMYVAMTRARRSVTILASEARPSIFVTELMNDPDYG VDSPVEATERTHTCLQCGGRLLYMPGQDGPGWYRCEHVKLCGNRMPACPECGVGLPVRDPKRSLMECSECDTSQQACPSC DAGWLIERRGRFGPFLGCVRFPDCNGKAKIAKTASR >Mature_916_residues MFSSSFALNLHGDKQVVLPAVNKAAANAFAQAFGQAWSKFNLRELAKEEERIQRLLQFLEKLQAPERYPSAFHIDPLARE ALNLTDRVLSKLNAEAVGDKTMRRIAPILSFSADPAKAREAAIERFVDAQLDRWTEFLDTVESMPLTPEQRLSVVVDEDA TLVLAGAGSGKTSVITAKAAYLVKAGIRSPEELLLLAFARDAATEMSERIEARCGVPIAARTFHALAYEIIGDVEGEKPA LAPTATDDKAFLSLIRDILRYIVSRVSDVAETVIGWFAGFFDDFPSPWDFKSAHEWYSEVESRNLRTLQGETVNSFEELI IANWLYRNGIAFEYEPTYEHKLTGTGRRVYTPDFRLTESGVYIEHFGVRKRRTSDGTEQLTTAPFVDRDEYLASMDWKRK VHTEHETVLIETYSWEREEGRLLDALAEKLAPHVTLRPIPDIEIYDQVTQVGVVDGFSSLIGTFLRHFKNGGYQIDDCSD KASTLKMGKRADAFLKIFGAVFREYQNRLGDRIDFEDMVIRATAHVERGRYNSPFRHILVDEFQDISTGRARLIKALKEQ HADAKVFAVGDDWQSIYRFVGSDIHIMRNFGAEFGGQFAGITGIHRTVDLGRTFRSVDKIALASRRFVLRNPAQITKTVR AAGETDQPAIQVAWTRRDNAENVLHETLASLTEASADGVQKPSVLLLGRYRFNEPDLRRLRRQHPALSLSFKTIHASKGL EADHVVILGADKARMGFPSMIVDDPLLSLVSPEAEPFENAEERRVMYVAMTRARRSVTILASEARPSIFVTELMNDPDYG VDSPVEATERTHTCLQCGGRLLYMPGQDGPGWYRCEHVKLCGNRMPACPECGVGLPVRDPKRSLMECSECDTSQQACPSC DAGWLIERRGRFGPFLGCVRFPDCNGKAKIAKTASR
Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]
COG id: COG0210
COG function: function code L; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]
Homologues:
Organism=Escherichia coli, GI1787196, Length=316, Percent_Identity=36.0759493670886, Blast_Score=186, Evalue=5e-48,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000212 - InterPro: IPR014016 - InterPro: IPR022161 - InterPro: IPR003661 [H]
Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 102765; Mature: 102765
Theoretical pI: Translated: 6.48; Mature: 6.48
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFSSSFALNLHGDKQVVLPAVNKAAANAFAQAFGQAWSKFNLRELAKEEERIQRLLQFLE CCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH KLQAPERYPSAFHIDPLAREALNLTDRVLSKLNAEAVGDKTMRRIAPILSFSADPAKARE HHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHH AAIERFVDAQLDRWTEFLDTVESMPLTPEQRLSVVVDEDATLVLAGAGSGKTSVITAKAA HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCEEEEEECCCCCCEEEEHHHH YLVKAGIRSPEELLLLAFARDAATEMSERIEARCGVPIAARTFHALAYEIIGDVEGEKPA HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC LAPTATDDKAFLSLIRDILRYIVSRVSDVAETVIGWFAGFFDDFPSPWDFKSAHEWYSEV CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH ESRNLRTLQGETVNSFEELIIANWLYRNGIAFEYEPTYEHKLTGTGRRVYTPDFRLTESG HCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCEEECCCCEEECCC VYIEHFGVRKRRTSDGTEQLTTAPFVDRDEYLASMDWKRKVHTEHETVLIETYSWEREEG EEEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHHHCCCCCEEEEEEECCCCCCH RLLDALAEKLAPHVTLRPIPDIEIYDQVTQVGVVDGFSSLIGTFLRHFKNGGYQIDDCSD HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC KASTLKMGKRADAFLKIFGAVFREYQNRLGDRIDFEDMVIRATAHVERGRYNSPFRHILV HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH DEFQDISTGRARLIKALKEQHADAKVFAVGDDWQSIYRFVGSDIHIMRNFGAEFGGQFAG HHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCHHHCCCCCC ITGIHRTVDLGRTFRSVDKIALASRRFVLRNPAQITKTVRAAGETDQPAIQVAWTRRDNA CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCH ENVLHETLASLTEASADGVQKPSVLLLGRYRFNEPDLRRLRRQHPALSLSFKTIHASKGL HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCC EADHVVILGADKARMGFPSMIVDDPLLSLVSPEAEPFENAEERRVMYVAMTRARRSVTIL CCCEEEEEECCCCCCCCCCHHHCCHHHHHHCCCCCCCCCHHHHEEEEEEEHHCCCEEEEE ASEARPSIFVTELMNDPDYGVDSPVEATERTHTCLQCGGRLLYMPGQDGPGWYRCEHVKL EECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCEEEHHHHH CGNRMPACPECGVGLPVRDPKRSLMECSECDTSQQACPSCDAGWLIERRGRFGPFLGCVR HCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCHHHCCCCCCCCCCEEE FPDCNGKAKIAKTASR CCCCCCCCCCCCCCCC >Mature Secondary Structure MFSSSFALNLHGDKQVVLPAVNKAAANAFAQAFGQAWSKFNLRELAKEEERIQRLLQFLE CCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH KLQAPERYPSAFHIDPLAREALNLTDRVLSKLNAEAVGDKTMRRIAPILSFSADPAKARE HHCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHH AAIERFVDAQLDRWTEFLDTVESMPLTPEQRLSVVVDEDATLVLAGAGSGKTSVITAKAA HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCEEEEEECCCCCCEEEEHHHH YLVKAGIRSPEELLLLAFARDAATEMSERIEARCGVPIAARTFHALAYEIIGDVEGEKPA HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC LAPTATDDKAFLSLIRDILRYIVSRVSDVAETVIGWFAGFFDDFPSPWDFKSAHEWYSEV CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH ESRNLRTLQGETVNSFEELIIANWLYRNGIAFEYEPTYEHKLTGTGRRVYTPDFRLTESG HCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCEEECCCCEEECCC VYIEHFGVRKRRTSDGTEQLTTAPFVDRDEYLASMDWKRKVHTEHETVLIETYSWEREEG EEEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHHHCCCCCEEEEEEECCCCCCH RLLDALAEKLAPHVTLRPIPDIEIYDQVTQVGVVDGFSSLIGTFLRHFKNGGYQIDDCSD HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC KASTLKMGKRADAFLKIFGAVFREYQNRLGDRIDFEDMVIRATAHVERGRYNSPFRHILV HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH DEFQDISTGRARLIKALKEQHADAKVFAVGDDWQSIYRFVGSDIHIMRNFGAEFGGQFAG HHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCHHHCCCCCC ITGIHRTVDLGRTFRSVDKIALASRRFVLRNPAQITKTVRAAGETDQPAIQVAWTRRDNA CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCH ENVLHETLASLTEASADGVQKPSVLLLGRYRFNEPDLRRLRRQHPALSLSFKTIHASKGL HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCC EADHVVILGADKARMGFPSMIVDDPLLSLVSPEAEPFENAEERRVMYVAMTRARRSVTIL CCCEEEEEECCCCCCCCCCHHHCCHHHHHHCCCCCCCCCHHHHEEEEEEEHHCCCEEEEE ASEARPSIFVTELMNDPDYGVDSPVEATERTHTCLQCGGRLLYMPGQDGPGWYRCEHVKL EECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCEEEHHHHH CGNRMPACPECGVGLPVRDPKRSLMECSECDTSQQACPSCDAGWLIERRGRFGPFLGCVR HCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCHHHCCCCCCCCCCEEE FPDCNGKAKIAKTASR CCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2542273; 8905232; 9278503 [H]