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The map label for this gene is ilvB [H]

Identifier: 110679694

GI number: 110679694

Start: 2336822

End: 2338441

Strand: Reverse

Name: ilvB [H]

Synonym: RD1_2449

Alternate gene names: 110679694

Gene position: 2338441-2336822 (Counterclockwise)

Preceding gene: 110679695

Following gene: 110679683

Centisome position: 56.58

GC content: 59.32

Gene sequence:

>1620_bases
ATGAACATCGCAGAATTGATCGTCGCAAGTTTGGAGAAAGCGGGCGTCATTGCGGCTTTTGGCGTTCCGGGTGAGGAGAA
CACACATCTTATGCAGGCTCTGGAAGCCTCGAGGATTGAGGTCATCCTGACGCGACATGAGCAAGCCGCGGCATTCATGG
CAAGCGTGCACGCGCGGATAACCGGAAAACCTGCCATGTGTTTTGCGACACTGGGTCCGGGCGCCACAAACCTGATAACC
GGCGTGGCGGATGCTCAACTGGATCATGCCCCCATGTTCGTTGTCACAGGTCAGGGCGCACGAGAGCGGATCGGCAAAAC
CGTGTCACATCAATTGATTGATCTTGAACGGCTCTTCAAACCCGTGACCAAATTCAGCCGGACATTGATGCACAAAGATG
AAGTGTCCGGCCTTGTGGCCGAGGCTTTGCGGCGCGCACAGGACCCCCATCCCGGCGCGGTGCATCTGTCGCTGCCGGAG
GATCTGGCCGGTGAGCAAACAGACGCACGACCCAAGAGCGCCCCGGAACCGCTACCCGCAACGGCGGCTCCCAAGGCGAT
CGGTGACGCACTCGAGATATTGAAATCAGCGCAACACCCGATGATCGTTGCAGGGAACGGGGTCATCCGCGCGCGCGCCG
GGCATGACGTTCAGCTTTTCGCCGAAGCCACGGGCATACCGCTCGCGACGACCTTCATGGCCAAGGGCATTTTGCCCGCA
GATCATCCGCTTCATCTGCATTGTGTCGGACAGCCCTTCGAAGACCATATCGACAAAGCGTTCCGGACCCGGGATCTCAT
CATCGCCGTCGGCTTTGATCCCGTGGAAGTCCCCCCGCAGAGCATCACAGCAGGCGGGGCAATCCCGGTTCTGCATGTGG
GTGAAAATCCCGCCGCGCTGGAACACGACTGGTTTATCCGTGGCGATGTTGCAGGTGACGTTGCAAAAACCATGCGTAGC
CTGACCGCGGGGCTCGATGGCAGGACGTGGGAAGAGGATGACCGGATCGTCGCCGTGCGCAAAGAGATCACTGAGCAGCG
CACCCGGCATCATGGGTCTGATGACAACGCCCTGCATCCCGCAGATGTACTGCGCAAGATCGAGGCGTCGTTGACGCACG
ACACATTCGTGGTGTCGGGAGTGGGCACTCACAAGATGGAGGTCGCGCGTTACCTTGCAGCGCGCAAGCCCAATCAGATC
ATCATTCCAAATGGGCTCGCGGGGATGGGCCTCGCCCTGCCCGGCGCCATTGCTGCGGCCCACCTTGGCCGCCATGGCCG
GGTTATGGCCATTTGTGGCGACGGGGAGTTCCTGATGAACGTCCAGGAGATGGAGACCGCTGACAGGCTGGGTCTTTCGC
TGACTGTGGTCATGTTCGAAGACAAGGGATATGGGCTTATCAAGGCAAAGCAGCAAGCGGACACCGGCGGCCACACGCCG
CTTTCGTTTGGCAACCCCGAATGGGATTCATTGGCAACGTCATTCGGCTGGCAGCACATTCGCACAGAAACCCTCGAAAC
ACTTGAAACGGCGCTGGAGGATCAACGTACAGGTCTGACACTCATCACGCTCCCGATCGATTACGGCGATGCGCTGAAGA
AAGACAGTTCACCTGTCTGA

Upstream 100 bases:

>100_bases
AAGAAGCAGGTGTCGGCAAAACCATTGACTACGCCCGCAGCGCGAACATCGCAGCCTATCGCCGTGTCGCAGATGCGGTC
ACTGCTTTGGGGACAGCGGC

Downstream 100 bases:

>100_bases
ACCCTGTGTCATTGCGTCCTGAGCAGCGGTCTTATCGCCTGTCTTTTTTAAACCGCGCGGAAAGTAACTGGCGTGGTGTT
TCAACATGGATCGTTTGGGC

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MNIAELIVASLEKAGVIAAFGVPGEENTHLMQALEASRIEVILTRHEQAAAFMASVHARITGKPAMCFATLGPGATNLIT
GVADAQLDHAPMFVVTGQGARERIGKTVSHQLIDLERLFKPVTKFSRTLMHKDEVSGLVAEALRRAQDPHPGAVHLSLPE
DLAGEQTDARPKSAPEPLPATAAPKAIGDALEILKSAQHPMIVAGNGVIRARAGHDVQLFAEATGIPLATTFMAKGILPA
DHPLHLHCVGQPFEDHIDKAFRTRDLIIAVGFDPVEVPPQSITAGGAIPVLHVGENPAALEHDWFIRGDVAGDVAKTMRS
LTAGLDGRTWEEDDRIVAVRKEITEQRTRHHGSDDNALHPADVLRKIEASLTHDTFVVSGVGTHKMEVARYLAARKPNQI
IIPNGLAGMGLALPGAIAAAHLGRHGRVMAICGDGEFLMNVQEMETADRLGLSLTVVMFEDKGYGLIKAKQQADTGGHTP
LSFGNPEWDSLATSFGWQHIRTETLETLETALEDQRTGLTLITLPIDYGDALKKDSSPV

Sequences:

>Translated_539_residues
MNIAELIVASLEKAGVIAAFGVPGEENTHLMQALEASRIEVILTRHEQAAAFMASVHARITGKPAMCFATLGPGATNLIT
GVADAQLDHAPMFVVTGQGARERIGKTVSHQLIDLERLFKPVTKFSRTLMHKDEVSGLVAEALRRAQDPHPGAVHLSLPE
DLAGEQTDARPKSAPEPLPATAAPKAIGDALEILKSAQHPMIVAGNGVIRARAGHDVQLFAEATGIPLATTFMAKGILPA
DHPLHLHCVGQPFEDHIDKAFRTRDLIIAVGFDPVEVPPQSITAGGAIPVLHVGENPAALEHDWFIRGDVAGDVAKTMRS
LTAGLDGRTWEEDDRIVAVRKEITEQRTRHHGSDDNALHPADVLRKIEASLTHDTFVVSGVGTHKMEVARYLAARKPNQI
IIPNGLAGMGLALPGAIAAAHLGRHGRVMAICGDGEFLMNVQEMETADRLGLSLTVVMFEDKGYGLIKAKQQADTGGHTP
LSFGNPEWDSLATSFGWQHIRTETLETLETALEDQRTGLTLITLPIDYGDALKKDSSPV
>Mature_539_residues
MNIAELIVASLEKAGVIAAFGVPGEENTHLMQALEASRIEVILTRHEQAAAFMASVHARITGKPAMCFATLGPGATNLIT
GVADAQLDHAPMFVVTGQGARERIGKTVSHQLIDLERLFKPVTKFSRTLMHKDEVSGLVAEALRRAQDPHPGAVHLSLPE
DLAGEQTDARPKSAPEPLPATAAPKAIGDALEILKSAQHPMIVAGNGVIRARAGHDVQLFAEATGIPLATTFMAKGILPA
DHPLHLHCVGQPFEDHIDKAFRTRDLIIAVGFDPVEVPPQSITAGGAIPVLHVGENPAALEHDWFIRGDVAGDVAKTMRS
LTAGLDGRTWEEDDRIVAVRKEITEQRTRHHGSDDNALHPADVLRKIEASLTHDTFVVSGVGTHKMEVARYLAARKPNQI
IIPNGLAGMGLALPGAIAAAHLGRHGRVMAICGDGEFLMNVQEMETADRLGLSLTVVMFEDKGYGLIKAKQQADTGGHTP
LSFGNPEWDSLATSFGWQHIRTETLETLETALEDQRTGLTLITLPIDYGDALKKDSSPV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=537, Percent_Identity=24.9534450651769, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=23.943661971831, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1790104, Length=534, Percent_Identity=29.7752808988764, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI87081685, Length=527, Percent_Identity=29.2220113851992, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786717, Length=512, Percent_Identity=28.125, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788716, Length=561, Percent_Identity=25.3119429590018, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787096, Length=524, Percent_Identity=23.8549618320611, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=25.6781193490054, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=25.6781193490054, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=567, Percent_Identity=23.9858906525573, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=553, Percent_Identity=26.0397830018083, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=491, Percent_Identity=23.6252545824847, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19922626, Length=479, Percent_Identity=25.2609603340292, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57770; Mature: 57770

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAELIVASLEKAGVIAAFGVPGEENTHLMQALEASRIEVILTRHEQAAAFMASVHARI
CCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHC
TGKPAMCFATLGPGATNLITGVADAQLDHAPMFVVTGQGARERIGKTVSHQLIDLERLFK
CCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
PVTKFSRTLMHKDEVSGLVAEALRRAQDPHPGAVHLSLPEDLAGEQTDARPKSAPEPLPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCC
TAAPKAIGDALEILKSAQHPMIVAGNGVIRARAGHDVQLFAEATGIPLATTFMAKGILPA
CCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCC
DHPLHLHCVGQPFEDHIDKAFRTRDLIIAVGFDPVEVPPQSITAGGAIPVLHVGENPAAL
CCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCC
EHDWFIRGDVAGDVAKTMRSLTAGLDGRTWEEDDRIVAVRKEITEQRTRHHGSDDNALHP
CCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCH
ADVLRKIEASLTHDTFVVSGVGTHKMEVARYLAARKPNQIIIPNGLAGMGLALPGAIAAA
HHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
HLGRHGRVMAICGDGEFLMNVQEMETADRLGLSLTVVMFEDKGYGLIKAKQQADTGGHTP
HCCCCCCEEEEECCCHHEEEHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCCCCCCC
LSFGNPEWDSLATSFGWQHIRTETLETLETALEDQRTGLTLITLPIDYGDALKKDSSPV
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCC
>Mature Secondary Structure
MNIAELIVASLEKAGVIAAFGVPGEENTHLMQALEASRIEVILTRHEQAAAFMASVHARI
CCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHC
TGKPAMCFATLGPGATNLITGVADAQLDHAPMFVVTGQGARERIGKTVSHQLIDLERLFK
CCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
PVTKFSRTLMHKDEVSGLVAEALRRAQDPHPGAVHLSLPEDLAGEQTDARPKSAPEPLPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCC
TAAPKAIGDALEILKSAQHPMIVAGNGVIRARAGHDVQLFAEATGIPLATTFMAKGILPA
CCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCC
DHPLHLHCVGQPFEDHIDKAFRTRDLIIAVGFDPVEVPPQSITAGGAIPVLHVGENPAAL
CCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCC
EHDWFIRGDVAGDVAKTMRSLTAGLDGRTWEEDDRIVAVRKEITEQRTRHHGSDDNALHP
CCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCH
ADVLRKIEASLTHDTFVVSGVGTHKMEVARYLAARKPNQIIIPNGLAGMGLALPGAIAAA
HHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
HLGRHGRVMAICGDGEFLMNVQEMETADRLGLSLTVVMFEDKGYGLIKAKQQADTGGHTP
HCCCCCCEEEEECCCHHEEEHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCCCCCCC
LSFGNPEWDSLATSFGWQHIRTETLETLETALEDQRTGLTLITLPIDYGDALKKDSSPV
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]