Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 110679695

GI number: 110679695

Start: 2338438

End: 2339787

Strand: Reverse

Name: gdhA [H]

Synonym: RD1_2450

Alternate gene names: 110679695

Gene position: 2339787-2338438 (Counterclockwise)

Preceding gene: 110679700

Following gene: 110679694

Centisome position: 56.61

GC content: 60.07

Gene sequence:

>1350_bases
ATGACAGGGCAAGAGCAGCTGACCGATAATCTGATGGAGAGGGTCGAAGCGCGCAACGCAGGACAGACTGAATTCCTTCA
AGCGGTGCGCGATGTGGCACGGGACGTCATGGCGGTTGAAAAAAACAATGCGCGCTGGAGCCATGCCAAGGTGCTTGAGC
GGCTGACCGAACCCGACCGCGTGATCGGCTTTCGTGTGGCGTGGATGGATGATCATGGGGATGTTCAGATCAATCGCGGC
TGGCGGGTCCAGACGTCCAACGCCATCGGGCCTTACAAGGGTGGCTTGCGGTTTCATCCGTCGGTCAATCCGTCGGTTCT
GAAGTTTCTCGGTTTTGAACAGGTCTTTAAGAACGCGCTCACGGGCTTGCCGCTTGGGGGTGCGAAAGGCGGCTCTGATT
TTGATCCGAACGGGCGCAGCGATGCCGAGATCATGCGCTTTTGCCAAGCCTTCATGCTGGAGCTTGCCCGCTACATCGGG
CCGGACGTGGATGTCCCCGCAGGCGATATTAACGTCGGGACGCGCGAAATCGGCTGGCTCTTTGGGACGTACAAGAAGGT
GAGTGGCGCATCCCATGGCGCATTGACCGGCAAGGGACATTCCTTTGGCGGCAGTGCCATGCGGGTCGAGGCCACGGGAT
ACGGGCTGATCTATTTCGTACAAGCCATGCTGGCGCAGTCCGGCAGTGCTCTTGACGGAAAACGGGTCGTGATCTCCGGC
AAGGGCAATGTGGCCACCCATGCAGCGGAAAAGGCAACATGCGAAGGAGCCAAGGTCGTCTGCCTGTCAGACACGTCGGG
TGTACTGCATGCACCCGATGGCATGTCGCATGACGCCATCACATGGGTGCGCGACCGCAAGGCACAGGGCGCGGACATTT
CCGACCCCCCGAAAGCACTTGACCTGTCCTTTGACGCGGGTAGCACGCCGTGGAGCGTGGCAGAGCACGACATCGCCCTG
CCCTGCGCCACGCAAAACGAGGTGGATGCAAAGGCCGCCAAAGATATCTGCGCAGCAGGCGCCACCCTGCTTGCGGAGGG
TGCGAACATGCCGTTGCAATCAAAGGCGCTGGAACACATCCGCACAGAAGGCCTGATGTATGCACCGGGCAAGGCCAGCA
ATGCGGGCGGCGTTGCGATCTCAGGTCTTGAGATGAGCCAGAACGCGCACCGCCGTTTCAACAGTGCAGAAGAGATTGAC
GCCGAACTGAAGTCGCTCATGGGCGATATACACGCCCGCCTTCTGGAAGAAGCAGGTGTCGGCAAAACCATTGACTACGC
CCGCAGCGCGAACATCGCAGCCTATCGCCGTGTCGCAGATGCGGTCACTGCTTTGGGGACAGCGGCATGA

Upstream 100 bases:

>100_bases
TGTTCCCAAAAAGCCGTTTTGCATTTGTGAGAAGAACGATGCGGAACTTTTCACGGACCACCCGCGTTTGACCGGGCAAT
CAAGAGTAAGGTGGACAAAA

Downstream 100 bases:

>100_bases
ACATCGCAGAATTGATCGTCGCAAGTTTGGAGAAAGCGGGCGTCATTGCGGCTTTTGGCGTTCCGGGTGAGGAGAACACA
CATCTTATGCAGGCTCTGGA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTGQEQLTDNLMERVEARNAGQTEFLQAVRDVARDVMAVEKNNARWSHAKVLERLTEPDRVIGFRVAWMDDHGDVQINRG
WRVQTSNAIGPYKGGLRFHPSVNPSVLKFLGFEQVFKNALTGLPLGGAKGGSDFDPNGRSDAEIMRFCQAFMLELARYIG
PDVDVPAGDINVGTREIGWLFGTYKKVSGASHGALTGKGHSFGGSAMRVEATGYGLIYFVQAMLAQSGSALDGKRVVISG
KGNVATHAAEKATCEGAKVVCLSDTSGVLHAPDGMSHDAITWVRDRKAQGADISDPPKALDLSFDAGSTPWSVAEHDIAL
PCATQNEVDAKAAKDICAAGATLLAEGANMPLQSKALEHIRTEGLMYAPGKASNAGGVAISGLEMSQNAHRRFNSAEEID
AELKSLMGDIHARLLEEAGVGKTIDYARSANIAAYRRVADAVTALGTAA

Sequences:

>Translated_449_residues
MTGQEQLTDNLMERVEARNAGQTEFLQAVRDVARDVMAVEKNNARWSHAKVLERLTEPDRVIGFRVAWMDDHGDVQINRG
WRVQTSNAIGPYKGGLRFHPSVNPSVLKFLGFEQVFKNALTGLPLGGAKGGSDFDPNGRSDAEIMRFCQAFMLELARYIG
PDVDVPAGDINVGTREIGWLFGTYKKVSGASHGALTGKGHSFGGSAMRVEATGYGLIYFVQAMLAQSGSALDGKRVVISG
KGNVATHAAEKATCEGAKVVCLSDTSGVLHAPDGMSHDAITWVRDRKAQGADISDPPKALDLSFDAGSTPWSVAEHDIAL
PCATQNEVDAKAAKDICAAGATLLAEGANMPLQSKALEHIRTEGLMYAPGKASNAGGVAISGLEMSQNAHRRFNSAEEID
AELKSLMGDIHARLLEEAGVGKTIDYARSANIAAYRRVADAVTALGTAA
>Mature_448_residues
TGQEQLTDNLMERVEARNAGQTEFLQAVRDVARDVMAVEKNNARWSHAKVLERLTEPDRVIGFRVAWMDDHGDVQINRGW
RVQTSNAIGPYKGGLRFHPSVNPSVLKFLGFEQVFKNALTGLPLGGAKGGSDFDPNGRSDAEIMRFCQAFMLELARYIGP
DVDVPAGDINVGTREIGWLFGTYKKVSGASHGALTGKGHSFGGSAMRVEATGYGLIYFVQAMLAQSGSALDGKRVVISGK
GNVATHAAEKATCEGAKVVCLSDTSGVLHAPDGMSHDAITWVRDRKAQGADISDPPKALDLSFDAGSTPWSVAEHDIALP
CATQNEVDAKAAKDICAAGATLLAEGANMPLQSKALEHIRTEGLMYAPGKASNAGGVAISGLEMSQNAHRRFNSAEEIDA
ELKSLMGDIHARLLEEAGVGKTIDYARSANIAAYRRVADAVTALGTAA

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=376, Percent_Identity=28.9893617021277, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4885281, Length=395, Percent_Identity=28.6075949367089, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI1788059, Length=440, Percent_Identity=52.2727272727273, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17544676, Length=353, Percent_Identity=29.4617563739377, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=445, Percent_Identity=44.7191011235955, Blast_Score=346, Evalue=5e-96,
Organism=Saccharomyces cerevisiae, GI6319256, Length=448, Percent_Identity=45.0892857142857, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24649283, Length=364, Percent_Identity=32.1428571428571, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45549226, Length=351, Percent_Identity=30.4843304843305, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553475, Length=351, Percent_Identity=30.4843304843305, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24649460, Length=289, Percent_Identity=30.4498269896194, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 47737; Mature: 47606

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQEQLTDNLMERVEARNAGQTEFLQAVRDVARDVMAVEKNNARWSHAKVLERLTEPDR
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
VIGFRVAWMDDHGDVQINRGWRVQTSNAIGPYKGGLRFHPSVNPSVLKFLGFEQVFKNAL
EEEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHH
TGLPLGGAKGGSDFDPNGRSDAEIMRFCQAFMLELARYIGPDVDVPAGDINVGTREIGWL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
FGTYKKVSGASHGALTGKGHSFGGSAMRVEATGYGLIYFVQAMLAQSGSALDGKRVVISG
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEC
KGNVATHAAEKATCEGAKVVCLSDTSGVLHAPDGMSHDAITWVRDRKAQGADISDPPKAL
CCCCHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCE
DLSFDAGSTPWSVAEHDIALPCATQNEVDAKAAKDICAAGATLLAEGANMPLQSKALEHI
EEECCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
RTEGLMYAPGKASNAGGVAISGLEMSQNAHRRFNSAEEIDAELKSLMGDIHARLLEEAGV
HHCCEEECCCCCCCCCCEEEECCHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GKTIDYARSANIAAYRRVADAVTALGTAA
CCCHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGQEQLTDNLMERVEARNAGQTEFLQAVRDVARDVMAVEKNNARWSHAKVLERLTEPDR
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
VIGFRVAWMDDHGDVQINRGWRVQTSNAIGPYKGGLRFHPSVNPSVLKFLGFEQVFKNAL
EEEEEEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHH
TGLPLGGAKGGSDFDPNGRSDAEIMRFCQAFMLELARYIGPDVDVPAGDINVGTREIGWL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
FGTYKKVSGASHGALTGKGHSFGGSAMRVEATGYGLIYFVQAMLAQSGSALDGKRVVISG
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEC
KGNVATHAAEKATCEGAKVVCLSDTSGVLHAPDGMSHDAITWVRDRKAQGADISDPPKAL
CCCCHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCE
DLSFDAGSTPWSVAEHDIALPCATQNEVDAKAAKDICAAGATLLAEGANMPLQSKALEHI
EEECCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
RTEGLMYAPGKASNAGGVAISGLEMSQNAHRRFNSAEEIDAELKSLMGDIHARLLEEAGV
HHCCEEECCCCCCCCCCEEEECCHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GKTIDYARSANIAAYRRVADAVTALGTAA
CCCHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10601858 [H]