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The map label for this gene is phnE [H]

Identifier: 110679650

GI number: 110679650

Start: 2288281

End: 2290470

Strand: Reverse

Name: phnE [H]

Synonym: RD1_2401

Alternate gene names: 110679650

Gene position: 2290470-2288281 (Counterclockwise)

Preceding gene: 110679651

Following gene: 110679649

Centisome position: 55.42

GC content: 60.55

Gene sequence:

>2190_bases
ATGGCTGACATTTCTACAGGCGGCGCGCCGCAAACCGTAGGCAGCATCCAGTCTGCCTATATGGCGCAGGTACAGCGCCG
CAGGCTTTATGGGGGTCTCACACTGCTGATATTCATCATTCTGATGGTGTCAGGTTTCAATGTCGCGGATGATCGCAATG
CCGGTGGTTTCTGGGAAGGTCTGCACCAGATTGGCGACTACCCCGCCGATGTTCTGTCCGAAGCGTGGGAGAAACGCGCC
GATCTGCCTGCGCTGATCGCCAAGTATTTTCCCGCCTTGGTGGAGACCATCAACATCGCCGCCGTTTCCACCCTGATCGG
GGCAATGGGCGGTTTGTTCCTGTCGCTGCTGGGCACGCGCGGGCTGGCGAAATGGCCGCGCCTGATCCCCTTGTTCCGCC
GCATTTCGGACATCATGCGCGCCGTGCCGGAAATCGTCATCGCGCTGGTGCTGATCTTTGTACTGGGGGGCGGCCCGGTG
CCCGCCATGATCGCCATCGCCATCCACACGGCAGGCGCTCTGGCCAAGATGTTTTCCGAAGTTTCCGAAAACACGGATCT
CAAGGCAGTCGAAGGGCTGTGCTCCACAGGTGCCACGTGGTCGCAACGGATGCTTCTGGGCGTGCTGCCACAGGTCGCAC
CGAACTACGTCAGCTACACGTTGCTCCGCTTTGAGATCAACATTCGTGCATCCGCCATCCTCGGCTTCGTTGGCGCGGGC
GGGCTGGGATATGAGCTGCGCAATTCCATGGCTTGGGGTCCGGGCCGATTTGATGAAGCGGCCGCGATTTTCATCCTGCT
GTTCGGCACGATCGTGTTCTTTGATCAACTCTCCAGCCGCTACCGCAACCGCTTGACCGAGGGGCAAGGCCCAATGACCA
CCACCGATCTGGACGCAACCAAGTTTGAGGCCGAAAAACTCTTTCACCGCAAGCGCTTGACTGGCTTCGGCATCCCCTTG
GTCCTCGCCGTCTACCTGGTCTATGTCTTCTTTGCGTTTGACCTGCCCGGGCTTGCCGGACGCGCCAATATGGAAAACGC
CGTGACGCTGGCTTCCGACAGCTGGTCCCACAAGGTGCATGTTACCCGGGACAACAGATCGGGAGAGATCACCTATGCCG
TCGAGGGAGAGCGCAAGGGCCGCTACCCCGAAGGTCAAAGACCCGATTGGGTAAGCGGCGATAATGCGATCATCATCGAT
CTGGGGCGCGGGCATATCGTGCGATATCTCCCGGACAACCGGACCGAGATCGAAATTCCCGGCTTCCCCCTGATCAGGGC
GCAGGCCGAGGGGCGCACCGTCACGACCAACCTGCGCGGTGATCTGCCCGATTGGATTTCCGCCTCTGACCGCCGCGTGG
GCATCACCACGCCCGAAGGCCGCATCACCCTGACCGGCGCGCGCACCGAAGTGTTCAATTATTTTCCCGGATGGGAGCTG
TTCTGGTTCACGCTCGACAGCCCCTATCATGGTCACAGCCTCGGCACGCTGTTGTTTGCAGACCGTCTGGACCCCGCAAG
ATCAAACATCTCCGGCGCGATTTCGGACTGGTGGAACAACGCCATGTGGCGGCACAGGGATGTGGCCTGGGCCATTGGCG
AGACGATCCTGATCGCTTTCCTTGGCACGATGGGGGCGGCCATCATCGCCCTCCCACTGGGGTTTCTCGCGGCCAAGCGG
TTTTCGCCCTTCATGTTGGAACGCGCCGCCACCCGCCGCCTGTTCGATTTTGTGCGCGGTGTCGATGCGGTGATCTGGAC
GGTGGTTCTGGCCCGCGCCTTTGGCCCCGGCCCGCTTACGGGCGCATTGGCAATCCTGATCACGGACACCGGCACCTTTG
GCAAGATCTTTTCGGAGGCGTTGGAAAACGTGGATCAAAAGCAGATCGAGGGTGTCGTCTCGACCGGGGCCAAACCCTTG
CAACGCTACCGCTTTGGCGTCATCCCGCAGATTGTGCCGGTGCTTTTGGCGCAAATCCTGTATTTTCTGGAATCCAACAC
CCGCTCCGCCACCATCATCGGCGCAATCACCGGCGGCGGCATCGGCCTGATGCTGACCCAAGCGATCCAGACCCAGAAAG
ACTGGGAGGAAGTCGCCTATTACATTGTGCTGATCATCGCCATGGTGATGTTCATGGACTGGTTCTCGGGCTGGCTGCGC
GGCAAGCTGATCGGGCGCAAGGACGCATGA

Upstream 100 bases:

>100_bases
ATCGTCGAAGCGCGCAAAGCGAAAATCGGCGGCTAAGCCCTTTTCATCACGCGTCCGGCAGGCCCTCGGGCCTGTCGGTA
TTTTTGTATCCGGGGGCAGC

Downstream 100 bases:

>100_bases
GTGGCAGCCTTCATGATTGAGCAGACCGATACTCCTCATGGGTGGAATAACGAGGAACGCGTGTTTTGAGTGCGACCAGA
TTGAAACCGGATGTCCCCTT

Product: phosphonate ABC transporter, permease protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MADISTGGAPQTVGSIQSAYMAQVQRRRLYGGLTLLIFIILMVSGFNVADDRNAGGFWEGLHQIGDYPADVLSEAWEKRA
DLPALIAKYFPALVETINIAAVSTLIGAMGGLFLSLLGTRGLAKWPRLIPLFRRISDIMRAVPEIVIALVLIFVLGGGPV
PAMIAIAIHTAGALAKMFSEVSENTDLKAVEGLCSTGATWSQRMLLGVLPQVAPNYVSYTLLRFEINIRASAILGFVGAG
GLGYELRNSMAWGPGRFDEAAAIFILLFGTIVFFDQLSSRYRNRLTEGQGPMTTTDLDATKFEAEKLFHRKRLTGFGIPL
VLAVYLVYVFFAFDLPGLAGRANMENAVTLASDSWSHKVHVTRDNRSGEITYAVEGERKGRYPEGQRPDWVSGDNAIIID
LGRGHIVRYLPDNRTEIEIPGFPLIRAQAEGRTVTTNLRGDLPDWISASDRRVGITTPEGRITLTGARTEVFNYFPGWEL
FWFTLDSPYHGHSLGTLLFADRLDPARSNISGAISDWWNNAMWRHRDVAWAIGETILIAFLGTMGAAIIALPLGFLAAKR
FSPFMLERAATRRLFDFVRGVDAVIWTVVLARAFGPGPLTGALAILITDTGTFGKIFSEALENVDQKQIEGVVSTGAKPL
QRYRFGVIPQIVPVLLAQILYFLESNTRSATIIGAITGGGIGLMLTQAIQTQKDWEEVAYYIVLIIAMVMFMDWFSGWLR
GKLIGRKDA

Sequences:

>Translated_729_residues
MADISTGGAPQTVGSIQSAYMAQVQRRRLYGGLTLLIFIILMVSGFNVADDRNAGGFWEGLHQIGDYPADVLSEAWEKRA
DLPALIAKYFPALVETINIAAVSTLIGAMGGLFLSLLGTRGLAKWPRLIPLFRRISDIMRAVPEIVIALVLIFVLGGGPV
PAMIAIAIHTAGALAKMFSEVSENTDLKAVEGLCSTGATWSQRMLLGVLPQVAPNYVSYTLLRFEINIRASAILGFVGAG
GLGYELRNSMAWGPGRFDEAAAIFILLFGTIVFFDQLSSRYRNRLTEGQGPMTTTDLDATKFEAEKLFHRKRLTGFGIPL
VLAVYLVYVFFAFDLPGLAGRANMENAVTLASDSWSHKVHVTRDNRSGEITYAVEGERKGRYPEGQRPDWVSGDNAIIID
LGRGHIVRYLPDNRTEIEIPGFPLIRAQAEGRTVTTNLRGDLPDWISASDRRVGITTPEGRITLTGARTEVFNYFPGWEL
FWFTLDSPYHGHSLGTLLFADRLDPARSNISGAISDWWNNAMWRHRDVAWAIGETILIAFLGTMGAAIIALPLGFLAAKR
FSPFMLERAATRRLFDFVRGVDAVIWTVVLARAFGPGPLTGALAILITDTGTFGKIFSEALENVDQKQIEGVVSTGAKPL
QRYRFGVIPQIVPVLLAQILYFLESNTRSATIIGAITGGGIGLMLTQAIQTQKDWEEVAYYIVLIIAMVMFMDWFSGWLR
GKLIGRKDA
>Mature_728_residues
ADISTGGAPQTVGSIQSAYMAQVQRRRLYGGLTLLIFIILMVSGFNVADDRNAGGFWEGLHQIGDYPADVLSEAWEKRAD
LPALIAKYFPALVETINIAAVSTLIGAMGGLFLSLLGTRGLAKWPRLIPLFRRISDIMRAVPEIVIALVLIFVLGGGPVP
AMIAIAIHTAGALAKMFSEVSENTDLKAVEGLCSTGATWSQRMLLGVLPQVAPNYVSYTLLRFEINIRASAILGFVGAGG
LGYELRNSMAWGPGRFDEAAAIFILLFGTIVFFDQLSSRYRNRLTEGQGPMTTTDLDATKFEAEKLFHRKRLTGFGIPLV
LAVYLVYVFFAFDLPGLAGRANMENAVTLASDSWSHKVHVTRDNRSGEITYAVEGERKGRYPEGQRPDWVSGDNAIIIDL
GRGHIVRYLPDNRTEIEIPGFPLIRAQAEGRTVTTNLRGDLPDWISASDRRVGITTPEGRITLTGARTEVFNYFPGWELF
WFTLDSPYHGHSLGTLLFADRLDPARSNISGAISDWWNNAMWRHRDVAWAIGETILIAFLGTMGAAIIALPLGFLAAKRF
SPFMLERAATRRLFDFVRGVDAVIWTVVLARAFGPGPLTGALAILITDTGTFGKIFSEALENVDQKQIEGVVSTGAKPLQ
RYRFGVIPQIVPVLLAQILYFLESNTRSATIIGAITGGGIGLMLTQAIQTQKDWEEVAYYIVLIIAMVMFMDWFSGWLRG
KLIGRKDA

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 80024; Mature: 79893

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADISTGGAPQTVGSIQSAYMAQVQRRRLYGGLTLLIFIILMVSGFNVADDRNAGGFWEG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LHQIGDYPADVLSEAWEKRADLPALIAKYFPALVETINIAAVSTLIGAMGGLFLSLLGTR
HHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLAKWPRLIPLFRRISDIMRAVPEIVIALVLIFVLGGGPVPAMIAIAIHTAGALAKMFSE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VSENTDLKAVEGLCSTGATWSQRMLLGVLPQVAPNYVSYTLLRFEINIRASAILGFVGAG
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEEEEEEEEEEHHHHHHHHCCC
GLGYELRNSMAWGPGRFDEAAAIFILLFGTIVFFDQLSSRYRNRLTEGQGPMTTTDLDAT
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
KFEAEKLFHRKRLTGFGIPLVLAVYLVYVFFAFDLPGLAGRANMENAVTLASDSWSHKVH
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEE
VTRDNRSGEITYAVEGERKGRYPEGQRPDWVSGDNAIIIDLGRGHIVRYLPDNRTEIEIP
EEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEECC
GFPLIRAQAEGRTVTTNLRGDLPDWISASDRRVGITTPEGRITLTGARTEVFNYFPGWEL
CCCEEEEECCCCEEEECCCCCCCHHHCCCCCEEEEECCCCEEEEEECHHHHHHCCCCCEE
FWFTLDSPYHGHSLGTLLFADRLDPARSNISGAISDWWNNAMWRHRDVAWAIGETILIAF
EEEEECCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHCCCCHHHCHHHHHHHHHHHHHH
LGTMGAAIIALPLGFLAAKRFSPFMLERAATRRLFDFVRGVDAVIWTVVLARAFGPGPLT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GALAILITDTGTFGKIFSEALENVDQKQIEGVVSTGAKPLQRYRFGVIPQIVPVLLAQIL
CEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
YFLESNTRSATIIGAITGGGIGLMLTQAIQTQKDWEEVAYYIVLIIAMVMFMDWFSGWLR
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKLIGRKDA
CCCCCCCCC
>Mature Secondary Structure 
ADISTGGAPQTVGSIQSAYMAQVQRRRLYGGLTLLIFIILMVSGFNVADDRNAGGFWEG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LHQIGDYPADVLSEAWEKRADLPALIAKYFPALVETINIAAVSTLIGAMGGLFLSLLGTR
HHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLAKWPRLIPLFRRISDIMRAVPEIVIALVLIFVLGGGPVPAMIAIAIHTAGALAKMFSE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VSENTDLKAVEGLCSTGATWSQRMLLGVLPQVAPNYVSYTLLRFEINIRASAILGFVGAG
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEEEEEEEEEEHHHHHHHHCCC
GLGYELRNSMAWGPGRFDEAAAIFILLFGTIVFFDQLSSRYRNRLTEGQGPMTTTDLDAT
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
KFEAEKLFHRKRLTGFGIPLVLAVYLVYVFFAFDLPGLAGRANMENAVTLASDSWSHKVH
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEE
VTRDNRSGEITYAVEGERKGRYPEGQRPDWVSGDNAIIIDLGRGHIVRYLPDNRTEIEIP
EEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEECC
GFPLIRAQAEGRTVTTNLRGDLPDWISASDRRVGITTPEGRITLTGARTEVFNYFPGWEL
CCCEEEEECCCCEEEECCCCCCCHHHCCCCCEEEEECCCCEEEEEECHHHHHHCCCCCEE
FWFTLDSPYHGHSLGTLLFADRLDPARSNISGAISDWWNNAMWRHRDVAWAIGETILIAF
EEEEECCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHCCCCHHHCHHHHHHHHHHHHHH
LGTMGAAIIALPLGFLAAKRFSPFMLERAATRRLFDFVRGVDAVIWTVVLARAFGPGPLT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GALAILITDTGTFGKIFSEALENVDQKQIEGVVSTGAKPLQRYRFGVIPQIVPVLLAQIL
CEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
YFLESNTRSATIIGAITGGGIGLMLTQAIQTQKDWEEVAYYIVLIIAMVMFMDWFSGWLR
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKLIGRKDA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]