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The map label for this gene is phnJ [H]

Identifier: 110679640

GI number: 110679640

Start: 2280421

End: 2281287

Strand: Reverse

Name: phnJ [H]

Synonym: RD1_2391

Alternate gene names: 110679640

Gene position: 2281287-2280421 (Counterclockwise)

Preceding gene: 110679641

Following gene: 110679639

Centisome position: 55.2

GC content: 59.98

Gene sequence:

>867_bases
ATGACCGAGTATAACTTCGCCTACCTGGACGAACAAACCAAACGGATGATCCGCCGTGCGATCCTAAAGGGTCTGGCGAT
CCCCGGCTATCAGGTCCCCTTCGCCTCGCGCGAGATGCCGATGCCCTATGGTTGGGGAACGGGCGGCGTGCAGGTCTCTG
CTGCGGTACTCACGCCCGAGGACAGGTTCAAGGTGATCGACCAAGGGGCCGATGACACCACAAACGCGGTTTCAATCCGG
ACCTTCTTTCAACGCACAGCGGGCGTTGCGACGACCACCGCAACTGGTGACGCCACCGTGATCCAGACCCGGCACCGCAT
CCCCGAAGAGCCGCTGCACGAGGGTCAGATCCTTGTTTATCAGGTGCCCATCCCCGAACCGCTGCGCTTTCTGGAACCGC
GCGAAAGCGAGACCCGCAAGATGCACAGCCTTGAGGAATATGGGCTCATGCATGTGAAGCTCTACGAGGATATCAGCCGC
CACGGCCATATCGCGACCTCTTACGCCTATCCGGTCAAGGTCGAGGGGCGCTACGTGATGGACCCCTCGCCGATCCCCAA
ATTCGACAACCCCAAACTGGGCAATATGGCCGCAATTCAGCTTTTTGGCGCGGGGCGCGAGCAACGTATTTACGCGCTGC
CGCCCTATACCCAAGTGGTCAGCCTCGACTTTGAGGATCACCCGTTCGAGCCGTGCAGGGCCGATCATGCTTGCGGCATG
TGTGGTGCAACCGAAAGCTATCTCGATGAGCTGATCATGGATGACGCAGGGGGGCGGATTTTTGTCTGTTCCGACACTGA
TTACTGCGAAGCGCGCCGCGCCGCGGGCCACATCGGCAAAGATAATGGCGGAAAGGACGCGGCATGA

Upstream 100 bases:

>100_bases
ATATCAAACTGCCCCACTACGTCGATTTTCAATCTGAACTGGAACTGGTGCGCAAGCTGCGCCGCGAAGCGGGTGCGCAA
CAGATGCAGGAGGCAGCGGA

Downstream 100 bases:

>100_bases
CCCCTTTGCTATCCGTTCAAGGCATCACCAAGCGATACGGCACATATATCGGCTGCGCGGATGTTTCGTTTGATCTCTAT
CCCGGTGAGGTTATGGGGAT

Product: phosphonate metabolism protein PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTEYNFAYLDEQTKRMIRRAILKGLAIPGYQVPFASREMPMPYGWGTGGVQVSAAVLTPEDRFKVIDQGADDTTNAVSIR
TFFQRTAGVATTTATGDATVIQTRHRIPEEPLHEGQILVYQVPIPEPLRFLEPRESETRKMHSLEEYGLMHVKLYEDISR
HGHIATSYAYPVKVEGRYVMDPSPIPKFDNPKLGNMAAIQLFGAGREQRIYALPPYTQVVSLDFEDHPFEPCRADHACGM
CGATESYLDELIMDDAGGRIFVCSDTDYCEARRAAGHIGKDNGGKDAA

Sequences:

>Translated_288_residues
MTEYNFAYLDEQTKRMIRRAILKGLAIPGYQVPFASREMPMPYGWGTGGVQVSAAVLTPEDRFKVIDQGADDTTNAVSIR
TFFQRTAGVATTTATGDATVIQTRHRIPEEPLHEGQILVYQVPIPEPLRFLEPRESETRKMHSLEEYGLMHVKLYEDISR
HGHIATSYAYPVKVEGRYVMDPSPIPKFDNPKLGNMAAIQLFGAGREQRIYALPPYTQVVSLDFEDHPFEPCRADHACGM
CGATESYLDELIMDDAGGRIFVCSDTDYCEARRAAGHIGKDNGGKDAA
>Mature_287_residues
TEYNFAYLDEQTKRMIRRAILKGLAIPGYQVPFASREMPMPYGWGTGGVQVSAAVLTPEDRFKVIDQGADDTTNAVSIRT
FFQRTAGVATTTATGDATVIQTRHRIPEEPLHEGQILVYQVPIPEPLRFLEPRESETRKMHSLEEYGLMHVKLYEDISRH
GHIATSYAYPVKVEGRYVMDPSPIPKFDNPKLGNMAAIQLFGAGREQRIYALPPYTQVVSLDFEDHPFEPCRADHACGMC
GATESYLDELIMDDAGGRIFVCSDTDYCEARRAAGHIGKDNGGKDAA

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=277, Percent_Identity=72.9241877256318, Blast_Score=440, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32096; Mature: 31964

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYNFAYLDEQTKRMIRRAILKGLAIPGYQVPFASREMPMPYGWGTGGVQVSAAVLTPE
CCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCH
DRFKVIDQGADDTTNAVSIRTFFQRTAGVATTTATGDATVIQTRHRIPEEPLHEGQILVY
HHHHEEECCCCCCCCEEHHHHHHHHHCCCEEEECCCCEEEEEHHHCCCCCCCCCCCEEEE
QVPIPEPLRFLEPRESETRKMHSLEEYGLMHVKLYEDISRHGHIATSYAYPVKVEGRYVM
ECCCCCHHHHCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHCCCEEEEEEEEEEECCEEEE
DPSPIPKFDNPKLGNMAAIQLFGAGREQRIYALPPYTQVVSLDFEDHPFEPCRADHACGM
CCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC
CGATESYLDELIMDDAGGRIFVCSDTDYCEARRAAGHIGKDNGGKDAA
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TEYNFAYLDEQTKRMIRRAILKGLAIPGYQVPFASREMPMPYGWGTGGVQVSAAVLTPE
CCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCH
DRFKVIDQGADDTTNAVSIRTFFQRTAGVATTTATGDATVIQTRHRIPEEPLHEGQILVY
HHHHEEECCCCCCCCEEHHHHHHHHHCCCEEEECCCCEEEEEHHHCCCCCCCCCCCEEEE
QVPIPEPLRFLEPRESETRKMHSLEEYGLMHVKLYEDISRHGHIATSYAYPVKVEGRYVM
ECCCCCHHHHCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHCCCEEEEEEEEEEECCEEEE
DPSPIPKFDNPKLGNMAAIQLFGAGREQRIYALPPYTQVVSLDFEDHPFEPCRADHACGM
CCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC
CGATESYLDELIMDDAGGRIFVCSDTDYCEARRAAGHIGKDNGGKDAA
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]