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The map label for this gene is cysB [H]

Identifier: 110679559

GI number: 110679559

Start: 2194307

End: 2195344

Strand: Direct

Name: cysB [H]

Synonym: RD1_2299

Alternate gene names: 110679559

Gene position: 2194307-2195344 (Clockwise)

Preceding gene: 110679558

Following gene: 110679560

Centisome position: 53.09

GC content: 60.12

Gene sequence:

>1038_bases
ATGCGTGTCGCGCGCGATCTTGAGCAGGCAATCGGAAACACCCCGCTGATCCGGCTGAAAGCCGCAAGTGAGGCAACAGG
GTGTGACATCCTCGGCAAGGCCGAATTCATGAACCCGGGGCAATCGGTCAAGGACCGCGCCGCGCTGTTCATCATCAAGG
ATGCCATCGCTAAGGGGACGCTCAAACCCGGCGGCACAATTGTCGAAGGGACCGCGGGCAATACCGGCATCGGTCTTGCG
CTTGTGGGGGCGTCGATGGGCTTCAAGACCGTGATCGTCATTCCCGAAACGCAAAGTCAGGAAAAAAAGGACATGTTGCG
GCTGGCCGGTGCGGAACTGGTGCAGGTGCCCGCAGCCCCTTATCGCAACCCCAACAACTTTGTGCGGTACTCGGAGCGTC
TAGCGCAGGAACTGGCGCGCACGACAAACGAAGGGGTGATCTGGGCCAACCAGTTTGACAATACCGCCAACAGGCAGGCG
CATATCGAAGGGACGGGGCCGGAAATCTGGGAACAGACAGGCGGCAAGGTCGATGGTTTCATCTGCGCCGTGGGCTCAGG
GGGAACGCTTGCGGGCGTGTCCGAAGCGCTCAAGCCCAAAGGGGTCAAGATCGGTCTGGTCGATCCGGACGGCGCGTCGC
TGTACAATTACTACACGCGCGGTGTGCTGGAGATGAGTGAAGGGTCCTCGATCAACGAAGGTATCGGGCAAATCCGGATC
ACGAGGAACCTTGAAGGCTTCACGCCTGACTTAAGCTATAATGTGCATGATGCAGAGGCTTTGCCCATCGTTTTTGACCT
GCTCGAACACGAGGGCCTGTGCCTTGGCGGCTCCTCCGGCATCAATATCGCGGGGGCCGTGCGCATGGCGCGCGACATGG
GGCCGGGGCATAGGATTGTGACCATCCTGTGTGATTACGGAACGCGCTATCAATCCAAGCTGTTCAACCCGGAGTTTCTG
GCGAGCAAGGGTCTGCCTGTCCCGGCGTGGCTGGACCGCGCGCCGGCCAGCATTCCGGGCGTGTTTGAAGACGTATGA

Upstream 100 bases:

>100_bases
TTTGTTCAGCCCGCGTGGCACTGGCATACCGCTAAGCCTTGAGGTCGGACGGGTGCCCCGATAGAGGTAGGTGAAATTCG
GCTAAAAAGAGGACAGGCTG

Downstream 100 bases:

>100_bases
GGGCGCTCATGCCTCTTTTGCTGGCGATGTTCCTCGCGCTGTGGGGGGCGATCGCGCAGGCGCAGGAACTGGCGGTGCGC
GACGAGGACGATTCGCTTTA

Product: cysteine synthase A

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRVARDLEQAIGNTPLIRLKAASEATGCDILGKAEFMNPGQSVKDRAALFIIKDAIAKGTLKPGGTIVEGTAGNTGIGLA
LVGASMGFKTVIVIPETQSQEKKDMLRLAGAELVQVPAAPYRNPNNFVRYSERLAQELARTTNEGVIWANQFDNTANRQA
HIEGTGPEIWEQTGGKVDGFICAVGSGGTLAGVSEALKPKGVKIGLVDPDGASLYNYYTRGVLEMSEGSSINEGIGQIRI
TRNLEGFTPDLSYNVHDAEALPIVFDLLEHEGLCLGGSSGINIAGAVRMARDMGPGHRIVTILCDYGTRYQSKLFNPEFL
ASKGLPVPAWLDRAPASIPGVFEDV

Sequences:

>Translated_345_residues
MRVARDLEQAIGNTPLIRLKAASEATGCDILGKAEFMNPGQSVKDRAALFIIKDAIAKGTLKPGGTIVEGTAGNTGIGLA
LVGASMGFKTVIVIPETQSQEKKDMLRLAGAELVQVPAAPYRNPNNFVRYSERLAQELARTTNEGVIWANQFDNTANRQA
HIEGTGPEIWEQTGGKVDGFICAVGSGGTLAGVSEALKPKGVKIGLVDPDGASLYNYYTRGVLEMSEGSSINEGIGQIRI
TRNLEGFTPDLSYNVHDAEALPIVFDLLEHEGLCLGGSSGINIAGAVRMARDMGPGHRIVTILCDYGTRYQSKLFNPEFL
ASKGLPVPAWLDRAPASIPGVFEDV
>Mature_345_residues
MRVARDLEQAIGNTPLIRLKAASEATGCDILGKAEFMNPGQSVKDRAALFIIKDAIAKGTLKPGGTIVEGTAGNTGIGLA
LVGASMGFKTVIVIPETQSQEKKDMLRLAGAELVQVPAAPYRNPNNFVRYSERLAQELARTTNEGVIWANQFDNTANRQA
HIEGTGPEIWEQTGGKVDGFICAVGSGGTLAGVSEALKPKGVKIGLVDPDGASLYNYYTRGVLEMSEGSSINEGIGQIRI
TRNLEGFTPDLSYNVHDAEALPIVFDLLEHEGLCLGGSSGINIAGAVRMARDMGPGHRIVTILCDYGTRYQSKLFNPEFL
ASKGLPVPAWLDRAPASIPGVFEDV

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=330, Percent_Identity=38.7878787878788, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI295821200, Length=330, Percent_Identity=38.7878787878788, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4557415, Length=330, Percent_Identity=38.7878787878788, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI2367138, Length=320, Percent_Identity=36.5625, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788754, Length=329, Percent_Identity=36.4741641337386, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17535051, Length=315, Percent_Identity=37.1428571428571, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566674, Length=312, Percent_Identity=36.5384615384615, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17562970, Length=312, Percent_Identity=36.5384615384615, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17534315, Length=331, Percent_Identity=32.9305135951662, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17561720, Length=330, Percent_Identity=33.9393939393939, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=33.4394904458599, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147552, Length=325, Percent_Identity=31.3846153846154, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566672, Length=220, Percent_Identity=33.6363636363636, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71996324, Length=333, Percent_Identity=26.1261261261261, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=37.9939209726444, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321594, Length=329, Percent_Identity=33.7386018237082, Blast_Score=162, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24643623, Length=334, Percent_Identity=34.4311377245509, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI20129101, Length=334, Percent_Identity=34.4311377245509, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 36698; Mature: 36698

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVARDLEQAIGNTPLIRLKAASEATGCDILGKAEFMNPGQSVKDRAALFIIKDAIAKGT
CCHHHHHHHHHCCCCEEEEEECCCCCCCCEECCHHHCCCCCCCHHHEEEEEEEHHHHCCC
LKPGGTIVEGTAGNTGIGLALVGASMGFKTVIVIPETQSQEKKDMLRLAGAELVQVPAAP
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHEECCCCC
YRNPNNFVRYSERLAQELARTTNEGVIWANQFDNTANRQAHIEGTGPEIWEQTGGKVDGF
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHCCCCCCEE
ICAVGSGGTLAGVSEALKPKGVKIGLVDPDGASLYNYYTRGVLEMSEGSSINEGIGQIRI
EEEECCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCCCCCCCEEEE
TRNLEGFTPDLSYNVHDAEALPIVFDLLEHEGLCLGGSSGINIAGAVRMARDMGPGHRIV
EECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCEEE
TILCDYGTRYQSKLFNPEFLASKGLPVPAWLDRAPASIPGVFEDV
EEEECCCCHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCCCHHCCC
>Mature Secondary Structure
MRVARDLEQAIGNTPLIRLKAASEATGCDILGKAEFMNPGQSVKDRAALFIIKDAIAKGT
CCHHHHHHHHHCCCCEEEEEECCCCCCCCEECCHHHCCCCCCCHHHEEEEEEEHHHHCCC
LKPGGTIVEGTAGNTGIGLALVGASMGFKTVIVIPETQSQEKKDMLRLAGAELVQVPAAP
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHEECCCCC
YRNPNNFVRYSERLAQELARTTNEGVIWANQFDNTANRQAHIEGTGPEIWEQTGGKVDGF
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHCCCCCCEE
ICAVGSGGTLAGVSEALKPKGVKIGLVDPDGASLYNYYTRGVLEMSEGSSINEGIGQIRI
EEEECCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCEEEECCCCCCCCCCCEEEE
TRNLEGFTPDLSYNVHDAEALPIVFDLLEHEGLCLGGSSGINIAGAVRMARDMGPGHRIV
EECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCEEE
TILCDYGTRYQSKLFNPEFLASKGLPVPAWLDRAPASIPGVFEDV
EEEECCCCHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]