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The map label for this gene is kefA [C]

Identifier: 110679560

GI number: 110679560

Start: 2195341

End: 2197677

Strand: Direct

Name: kefA [C]

Synonym: RD1_2300

Alternate gene names: 110679560

Gene position: 2195341-2197677 (Clockwise)

Preceding gene: 110679559

Following gene: 110679563

Centisome position: 53.12

GC content: 58.45

Gene sequence:

>2337_bases
ATGAGGGCGCTCATGCCTCTTTTGCTGGCGATGTTCCTCGCGCTGTGGGGGGCGATCGCGCAGGCGCAGGAACTGGCGGT
GCGCGACGAGGACGATTCGCTTTATTCCTTCTGGGAGGATGCGGCGTCCCGGGCAGAGCAACGCATCGACACAGCCGACG
ATACGAGCACCGAAGACCTCGAAACGCTGCGACAACGGCTCGCGACGTTCCGGGGCGAGTTCGATTTGGCACGACAGGCC
AATTCAGCGCGTATCGCGACCATCCGCTCGCAGATCAACGCTCTGGGACCCGTGCCGGAAAATGGCGAGGAATCCCCGGA
AATCGCTGCGACACGGGCCGAACTGGCGGGCCAGTTCGACGACCTGCAAGCTCCCATTCTGGCAGCGGATGTCGCATTTC
GTCGCGCGGATGGTCTCATCAATGAAATCGACACGATCCTGCGGGAGCGTGAAACGCGTCGTTTGCTGTCACTCGGGCCT
TCACCCCTGAACCCCAAAACATGGTCCGTTGCCTTTGGTGATACCGAACGCATTCTGCGTGATCTTGGCGATGATCTGTC
AGGCATCGCCAACACGGCCAATCAACGCCAACTCCGCAACAACCTGCCGCTTGTGGCTTTGCTGCTGGTCATTTCGCTGA
CCCTCATTGTGCGAGGACGCGTGTGGGCGGGGAAGGCCGTTAATTTCATGCGCCGCTTTGGTGGTCGAGGCTCCGGCGTA
TGGAGCTTTCTTGTGTCCTTGCTGCGCATTATCCTGCCGCTTTCCGGGGTATATATCCTTGCGCATGCGATTTTGCTGAC
CGCGCGTTTCGGCGACACCATCGAGGAATTGATCGCGCTTTTCCCTGTCTGGGGCCTCCTTTTGCTGGGTTTTCGCTGGC
TCGCGGAACGGCTTTTCGCCCGTGAGGAAGACGATGCCGTGATTTGCCTGACGCCTGAAGGGCGGCGCGCAGGTCGGTTT
TACATGTTCGTGCTGTCCGTTCTGTTCCTGTCGCATGGGTTGATTGCGGTTCTGTTTGACCTGGAACAGGCGGCGCCGGA
AACGCTTGCCGTGATCGCATTCCCGATCGTGGTTCTCTTTGGTTTCGTCCTCTTTCCGATGGGCTTCATGCTGCGGAAGT
ACGACCTGACGGACGAGTCAGAGGAAGCAGAGGATCACGCGCAGAAAGCCGGTGTGGGGCGTGTCATACGCTTCTTTGGG
CTCATCCTGATTGCAGTCAGTGTGATCGCCCCCTCAATGACGGCCATCGGGTATGCCGAGGCGGGCAACGCGATGCTGTA
TCCCACGATTCTGAGCATGCTGGTGCTGGGATTGGTCATGGTGCTTCAGCGCTTCGCGGCTGATGTCTACGGGCTGATTA
CGGGGCAGGGGGTGGAGGCGCGCGACAGTCTCATGGCGATATTCGCCGGCTTCGTACTGGTGCTGGCAAGCCTTCCTTTT
CTGGCGCTGCTCTGGGGCGCGCGCGTAACGGATCTGACCGAACTCTGGCGCAGGTTCCTGGACGGTTTTGAAATTGGCGG
CACGCGAATTTCGCCGTCGGATTTCCTGACGTTCGCCATTGTTTTTGTCATCGGCTACACCGTGACCCGCCTGTTGCAGG
GCACCTTGCGCACCAACGTGCTGCCGAAAACGCGGCTGGATATTGGCGGGCAGAATGCCCTGATTTCCGGCATTGGTTAC
GTTGGTATCTTTCTGGCGTCGCTGATTGCTGTAACGGTGGCGGGCGTCGATCTTTCCGCCTTTGCCATTGTCGCGGGGGC
CTTATCGGTTGGCATCGGTTTTGGCCTGCAGACCATCGTGTCCAATTTTGTGTCCGGAATTATCCTGTTGGTGGAACGGC
CCATTTCCGAGGGCGACTGGATCGAGGTGGGCGGTCAGATGGGGGTGGTCAAACACATCTCTGTCCGCTCCACCCGGATC
GAAACCTTCGACCGCACGGATGTCATCGTGCCGAACTCCGATCTGATTGCCGGGACGGTCACCAATTATACGCGCGGCAA
TACAATCGGGCGTCTGATCGTTAAAATTGGCGTCGCTTACGGCACCGACACCAAACGTGTTGACAGGATTTTGCGCGAGA
TCGCGAATGCGCAGCCCATGGTGCTGAACAATCCGCCGCCAAATGTGGTTTTCATGGGCTTTGGAGCGGATTCGCTGGAT
TTTGAAATCCGGGCCATCCTGCGCGATGTAAACTGGATTCTCTCGGTTCAGAATGACATCAACCACGAAATCGCCGCGCG
CTTTACCGAGGAGGGGATCGAAATACCCTTTGCACAGCGCGACGTTTGGTTGCGCAACCCGGAAGTCTTGCAAACAGGAT
CTGACAAAAGCGAGTGA

Upstream 100 bases:

>100_bases
CCAAGCTGTTCAACCCGGAGTTTCTGGCGAGCAAGGGTCTGCCTGTCCCGGCGTGGCTGGACCGCGCGCCGGCCAGCATT
CCGGGCGTGTTTGAAGACGT

Downstream 100 bases:

>100_bases
TGCCCGCTTTCCCGAATTTGCCTCTTGCAAAGAACCTCACCGCCGCGCTACACCGCGCCAACGGAGCGGTGGCCGAGTGG
TCGAAGGCGCACGCCTGGAA

Product: mechanosensitive ion channel protein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MRALMPLLLAMFLALWGAIAQAQELAVRDEDDSLYSFWEDAASRAEQRIDTADDTSTEDLETLRQRLATFRGEFDLARQA
NSARIATIRSQINALGPVPENGEESPEIAATRAELAGQFDDLQAPILAADVAFRRADGLINEIDTILRERETRRLLSLGP
SPLNPKTWSVAFGDTERILRDLGDDLSGIANTANQRQLRNNLPLVALLLVISLTLIVRGRVWAGKAVNFMRRFGGRGSGV
WSFLVSLLRIILPLSGVYILAHAILLTARFGDTIEELIALFPVWGLLLLGFRWLAERLFAREEDDAVICLTPEGRRAGRF
YMFVLSVLFLSHGLIAVLFDLEQAAPETLAVIAFPIVVLFGFVLFPMGFMLRKYDLTDESEEAEDHAQKAGVGRVIRFFG
LILIAVSVIAPSMTAIGYAEAGNAMLYPTILSMLVLGLVMVLQRFAADVYGLITGQGVEARDSLMAIFAGFVLVLASLPF
LALLWGARVTDLTELWRRFLDGFEIGGTRISPSDFLTFAIVFVIGYTVTRLLQGTLRTNVLPKTRLDIGGQNALISGIGY
VGIFLASLIAVTVAGVDLSAFAIVAGALSVGIGFGLQTIVSNFVSGIILLVERPISEGDWIEVGGQMGVVKHISVRSTRI
ETFDRTDVIVPNSDLIAGTVTNYTRGNTIGRLIVKIGVAYGTDTKRVDRILREIANAQPMVLNNPPPNVVFMGFGADSLD
FEIRAILRDVNWILSVQNDINHEIAARFTEEGIEIPFAQRDVWLRNPEVLQTGSDKSE

Sequences:

>Translated_778_residues
MRALMPLLLAMFLALWGAIAQAQELAVRDEDDSLYSFWEDAASRAEQRIDTADDTSTEDLETLRQRLATFRGEFDLARQA
NSARIATIRSQINALGPVPENGEESPEIAATRAELAGQFDDLQAPILAADVAFRRADGLINEIDTILRERETRRLLSLGP
SPLNPKTWSVAFGDTERILRDLGDDLSGIANTANQRQLRNNLPLVALLLVISLTLIVRGRVWAGKAVNFMRRFGGRGSGV
WSFLVSLLRIILPLSGVYILAHAILLTARFGDTIEELIALFPVWGLLLLGFRWLAERLFAREEDDAVICLTPEGRRAGRF
YMFVLSVLFLSHGLIAVLFDLEQAAPETLAVIAFPIVVLFGFVLFPMGFMLRKYDLTDESEEAEDHAQKAGVGRVIRFFG
LILIAVSVIAPSMTAIGYAEAGNAMLYPTILSMLVLGLVMVLQRFAADVYGLITGQGVEARDSLMAIFAGFVLVLASLPF
LALLWGARVTDLTELWRRFLDGFEIGGTRISPSDFLTFAIVFVIGYTVTRLLQGTLRTNVLPKTRLDIGGQNALISGIGY
VGIFLASLIAVTVAGVDLSAFAIVAGALSVGIGFGLQTIVSNFVSGIILLVERPISEGDWIEVGGQMGVVKHISVRSTRI
ETFDRTDVIVPNSDLIAGTVTNYTRGNTIGRLIVKIGVAYGTDTKRVDRILREIANAQPMVLNNPPPNVVFMGFGADSLD
FEIRAILRDVNWILSVQNDINHEIAARFTEEGIEIPFAQRDVWLRNPEVLQTGSDKSE
>Mature_778_residues
MRALMPLLLAMFLALWGAIAQAQELAVRDEDDSLYSFWEDAASRAEQRIDTADDTSTEDLETLRQRLATFRGEFDLARQA
NSARIATIRSQINALGPVPENGEESPEIAATRAELAGQFDDLQAPILAADVAFRRADGLINEIDTILRERETRRLLSLGP
SPLNPKTWSVAFGDTERILRDLGDDLSGIANTANQRQLRNNLPLVALLLVISLTLIVRGRVWAGKAVNFMRRFGGRGSGV
WSFLVSLLRIILPLSGVYILAHAILLTARFGDTIEELIALFPVWGLLLLGFRWLAERLFAREEDDAVICLTPEGRRAGRF
YMFVLSVLFLSHGLIAVLFDLEQAAPETLAVIAFPIVVLFGFVLFPMGFMLRKYDLTDESEEAEDHAQKAGVGRVIRFFG
LILIAVSVIAPSMTAIGYAEAGNAMLYPTILSMLVLGLVMVLQRFAADVYGLITGQGVEARDSLMAIFAGFVLVLASLPF
LALLWGARVTDLTELWRRFLDGFEIGGTRISPSDFLTFAIVFVIGYTVTRLLQGTLRTNVLPKTRLDIGGQNALISGIGY
VGIFLASLIAVTVAGVDLSAFAIVAGALSVGIGFGLQTIVSNFVSGIILLVERPISEGDWIEVGGQMGVVKHISVRSTRI
ETFDRTDVIVPNSDLIAGTVTNYTRGNTIGRLIVKIGVAYGTDTKRVDRILREIANAQPMVLNNPPPNVVFMGFGADSLD
FEIRAILRDVNWILSVQNDINHEIAARFTEEGIEIPFAQRDVWLRNPEVLQTGSDKSE

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=281, Percent_Identity=34.5195729537367, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI2367355, Length=254, Percent_Identity=29.5275590551181, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1789291, Length=214, Percent_Identity=31.3084112149533, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787591, Length=199, Percent_Identity=30.1507537688442, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 85310; Mature: 85310

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALMPLLLAMFLALWGAIAQAQELAVRDEDDSLYSFWEDAASRAEQRIDTADDTSTEDL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ETLRQRLATFRGEFDLARQANSARIATIRSQINALGPVPENGEESPEIAATRAELAGQFD
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHH
DLQAPILAADVAFRRADGLINEIDTILRERETRRLLSLGPSPLNPKTWSVAFGDTERILR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHH
DLGDDLSGIANTANQRQLRNNLPLVALLLVISLTLIVRGRVWAGKAVNFMRRFGGRGSGV
HHCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
WSFLVSLLRIILPLSGVYILAHAILLTARFGDTIEELIALFPVWGLLLLGFRWLAERLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
REEDDAVICLTPEGRRAGRFYMFVLSVLFLSHGLIAVLFDLEQAAPETLAVIAFPIVVLF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
GFVLFPMGFMLRKYDLTDESEEAEDHAQKAGVGRVIRFFGLILIAVSVIAPSMTAIGYAE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEHHC
AGNAMLYPTILSMLVLGLVMVLQRFAADVYGLITGQGVEARDSLMAIFAGFVLVLASLPF
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LALLWGARVTDLTELWRRFLDGFEIGGTRISPSDFLTFAIVFVIGYTVTRLLQGTLRTNV
HHHHHCCHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPKTRLDIGGQNALISGIGYVGIFLASLIAVTVAGVDLSAFAIVAGALSVGIGFGLQTIV
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
SNFVSGIILLVERPISEGDWIEVGGQMGVVKHISVRSTRIETFDRTDVIVPNSDLIAGTV
HHHHHHHHHHHHCCCCCCCEEEECCCCCHHHEEHHHHHHHCCCCCCCEEECCCCEEEECC
TNYTRGNTIGRLIVKIGVAYGTDTKRVDRILREIANAQPMVLNNPPPNVVFMGFGADSLD
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCCCH
FEIRAILRDVNWILSVQNDINHEIAARFTEEGIEIPFAQRDVWLRNPEVLQTGSDKSE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHEECCCCCCC
>Mature Secondary Structure
MRALMPLLLAMFLALWGAIAQAQELAVRDEDDSLYSFWEDAASRAEQRIDTADDTSTEDL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ETLRQRLATFRGEFDLARQANSARIATIRSQINALGPVPENGEESPEIAATRAELAGQFD
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHH
DLQAPILAADVAFRRADGLINEIDTILRERETRRLLSLGPSPLNPKTWSVAFGDTERILR
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHH
DLGDDLSGIANTANQRQLRNNLPLVALLLVISLTLIVRGRVWAGKAVNFMRRFGGRGSGV
HHCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
WSFLVSLLRIILPLSGVYILAHAILLTARFGDTIEELIALFPVWGLLLLGFRWLAERLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
REEDDAVICLTPEGRRAGRFYMFVLSVLFLSHGLIAVLFDLEQAAPETLAVIAFPIVVLF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
GFVLFPMGFMLRKYDLTDESEEAEDHAQKAGVGRVIRFFGLILIAVSVIAPSMTAIGYAE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEHHC
AGNAMLYPTILSMLVLGLVMVLQRFAADVYGLITGQGVEARDSLMAIFAGFVLVLASLPF
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
LALLWGARVTDLTELWRRFLDGFEIGGTRISPSDFLTFAIVFVIGYTVTRLLQGTLRTNV
HHHHHCCHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPKTRLDIGGQNALISGIGYVGIFLASLIAVTVAGVDLSAFAIVAGALSVGIGFGLQTIV
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
SNFVSGIILLVERPISEGDWIEVGGQMGVVKHISVRSTRIETFDRTDVIVPNSDLIAGTV
HHHHHHHHHHHHCCCCCCCEEEECCCCCHHHEEHHHHHHHCCCCCCCEEECCCCEEEECC
TNYTRGNTIGRLIVKIGVAYGTDTKRVDRILREIANAQPMVLNNPPPNVVFMGFGADSLD
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCCCH
FEIRAILRDVNWILSVQNDINHEIAARFTEEGIEIPFAQRDVWLRNPEVLQTGSDKSE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]