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The map label for this gene is purN [H]

Identifier: 110679519

GI number: 110679519

Start: 2156613

End: 2157209

Strand: Direct

Name: purN [H]

Synonym: RD1_2253

Alternate gene names: 110679519

Gene position: 2156613-2157209 (Clockwise)

Preceding gene: 110679518

Following gene: 110679520

Centisome position: 52.18

GC content: 59.8

Gene sequence:

>597_bases
GTGACAAAACGGGTCGCTATTTTCATTTCCGGCGGGGGGTCGAACATGATCCGCCTGCTGGACAGCATGACCGGGGATCA
TCCCGCGCGCGTCTGCGTTGTCTTGTCCAACAACCCCAAGGCTGGTGGGCTGGAGCGGGCCGAAGAACGCGGCGTGCCAA
CCGAAATCGTGCGGCATCAACCTTTCGGGGCCGATACCTCCGGCTTTGAGCATGCGATTCTGGGTGCGCTGGCGGAGCAT
AAGCCCGATATCATTTGCCTCGCGGGGTTCATGCGGATCCTGACAGCCGAGTTTGTAAACAGATGGCGCGGCAAGATGCT
CAATATCCATCCCTCATTGCTGCCAAAGTACAAAGGGCTGCATACCCATGCGCGCGCCCTTGCTGCGGGAGATACCGTCC
ATGGCTGCACGGTTCACGAAGTGACACCGGCCCTCGATGATGGCCCGATATTGGGTCAGGCGCGGGTGCCTGTATTGGCA
GGGGATACCGCAGAAACGCTGGCCGCCAGAGTTCTGGTGCAGGAGCATATATTGTACCCTATGGTGCTGCGCCGATTTGT
CGGTGGTGACACGGCTCCCGTGTTTCTCGACGGGTGA

Upstream 100 bases:

>100_bases
CCGACAAGCTGACACAGTTGCTGGCCAGTCTGGGCGAGGATGTCGCGCGCATCGGACATGTCAGCGCGCGAGAGGGTGTG
CGCTATTCCGGCACGCTTTT

Downstream 100 bases:

>100_bases
TGGGTGGTGCTGCTGGTCAATGCCACCCGGCTCGTGTAACGTCCCGTTACGGGGTGGACGCAAACATCAAAAACAAAGAA
TTGCCCGAATGAAAACCATC

Product: phosphoribosylglycinamide formyltransferase putative

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MTKRVAIFISGGGSNMIRLLDSMTGDHPARVCVVLSNNPKAGGLERAEERGVPTEIVRHQPFGADTSGFEHAILGALAEH
KPDIICLAGFMRILTAEFVNRWRGKMLNIHPSLLPKYKGLHTHARALAAGDTVHGCTVHEVTPALDDGPILGQARVPVLA
GDTAETLAARVLVQEHILYPMVLRRFVGGDTAPVFLDG

Sequences:

>Translated_198_residues
MTKRVAIFISGGGSNMIRLLDSMTGDHPARVCVVLSNNPKAGGLERAEERGVPTEIVRHQPFGADTSGFEHAILGALAEH
KPDIICLAGFMRILTAEFVNRWRGKMLNIHPSLLPKYKGLHTHARALAAGDTVHGCTVHEVTPALDDGPILGQARVPVLA
GDTAETLAARVLVQEHILYPMVLRRFVGGDTAPVFLDG
>Mature_197_residues
TKRVAIFISGGGSNMIRLLDSMTGDHPARVCVVLSNNPKAGGLERAEERGVPTEIVRHQPFGADTSGFEHAILGALAEHK
PDIICLAGFMRILTAEFVNRWRGKMLNIHPSLLPKYKGLHTHARALAAGDTVHGCTVHEVTPALDDGPILGQARVPVLAG
DTAETLAARVLVQEHILYPMVLRRFVGGDTAPVFLDG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=187, Percent_Identity=47.0588235294118, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869995, Length=187, Percent_Identity=47.0588235294118, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869993, Length=187, Percent_Identity=47.0588235294118, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788846, Length=187, Percent_Identity=44.3850267379679, Blast_Score=164, Evalue=2e-42,
Organism=Escherichia coli, GI1787483, Length=149, Percent_Identity=32.2147651006711, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=178, Percent_Identity=40.4494382022472, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=30.3030303030303, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=45.0261780104712, Blast_Score=159, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21280; Mature: 21149

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRVAIFISGGGSNMIRLLDSMTGDHPARVCVVLSNNPKAGGLERAEERGVPTEIVRHQ
CCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHCCCCHHHHHCC
PFGADTSGFEHAILGALAEHKPDIICLAGFMRILTAEFVNRWRGKMLNIHPSLLPKYKGL
CCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCH
HTHARALAAGDTVHGCTVHEVTPALDDGPILGQARVPVLAGDTAETLAARVLVQEHILYP
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
MVLRRFVGGDTAPVFLDG
HHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
TKRVAIFISGGGSNMIRLLDSMTGDHPARVCVVLSNNPKAGGLERAEERGVPTEIVRHQ
CCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHCCCCHHHHHCC
PFGADTSGFEHAILGALAEHKPDIICLAGFMRILTAEFVNRWRGKMLNIHPSLLPKYKGL
CCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCH
HTHARALAAGDTVHGCTVHEVTPALDDGPILGQARVPVLAGDTAETLAARVLVQEHILYP
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
MVLRRFVGGDTAPVFLDG
HHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]