Click here to switch to the map view.

The map label for this gene is cheB

Identifier: 110679513

GI number: 110679513

Start: 2148669

End: 2149796

Strand: Direct

Name: cheB

Synonym: RD1_2244

Alternate gene names: 110679513

Gene position: 2148669-2149796 (Clockwise)

Preceding gene: 110679512

Following gene: 110679514

Centisome position: 51.99

GC content: 57.8

Gene sequence:

>1128_bases
TTGCACAGCGATACGAACGAGCATCAATCTCACGTACCGGCCAAGAAGGTCTTGATTGTTGACGATTCGCGTGTGATCCG
GATGTGGCTGCGCACAGTATTGGCCTCGGACAGACGCCTAGAAGTTGTCGGGGAGGCAAGCGATGCCGTTGAAGCGCGCG
ATTACATCCGCGCCAATCCGGCCGACGTCATAACGCTGGACATCGAAATGCCGGGGATGAGCGGTCTGGAGTTTTTGACG
CGCCTGATGCGCGCGCGCCCTATGCCGGTTGTCATGCTGTCCAGCCTGACAAGCAAAGGCAGCGATGCGGCTGTTCAGGC
GCTTTCACGCGGTGCGATCGACTGTATTTTGAAACCGACAGATGGGTATGACCTTGATCTTTCGCGCGACATTTGTGAGC
GGGTCTATCAGGCGGCCTGCACGCGTCCGGCTTCCCTGAAAAGGGGCGAGGCGACAGAAAAACCAGCAGATGCGCCCATG
ATGCAGCGCGCTTCCGCGCTGGGTCAGCCTTGTCGCAGGGGGTCGCTGATCTTGATTGGCGCATCAACAGGCGGCGTGTC
AGCCTTGGAAGCCGTGTTGCCAAAGCTTGACCCTGACGGTGCACCCGTCGTCATCGTTCAGCACATGCCCGGTAACTTTC
TTGAAAGCTTTTCCCAAAGGCTGGACAGACAACTGCCGCAGAACGTGAAACTCGCCGAAGAAGGCGTGCCGCTGCGTCGG
GGTGATGTGATCCTAGCGCCCGGGATCGGAAAACACACGGAAGTCGTAAGGCGCATGGATGGCTGGCAGTGTCGGTTTGT
TGCGAATGATCCCGCCGCCTTGCACTGCCCATCGGTTGATACCCTGTTTTTATCCGCTGTTTCCGAAGCTAAAAAAGTGT
CCGTTGCGATCCTGACAGGTCTGGGGAAGGATGGTGCGCAAGGCATGTTGAAATTGGCAGAGGCTGGTGCCACCACATTC
GGCCAGGATGAGGAAACATCCGTGGTTTACGGCATGCCGCGCGTCGCTTTCGGTCTGGGCGCTGTCCAGCATCAACTGCC
AATCCACAAAATCGGTGAAGCGCTGCGAGAGAGCAAGAACGCATCGGCACGAACAGCGCGCAGAACGACCAAGGTACCTT
TGCAATGA

Upstream 100 bases:

>100_bases
CACGACCATTAAGGCTCAATTTACATCTGTGCGGCATGTTTGGACAAGTGCGTGTCACATCAGGCATGCTTTGGGACCCC
AAACGGAAAAGAGCATGGTT

Downstream 100 bases:

>100_bases
CAAAAAACCGTCAACATATAACTCAGGGTGAACGTGCGGTTTCCGCTGATCCTGATCGGGCAATCTCCACAATTCTAGGG
TCTTGCGTTTCATGCTGTCT

Product: chemotaxis response regulator protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MHSDTNEHQSHVPAKKVLIVDDSRVIRMWLRTVLASDRRLEVVGEASDAVEARDYIRANPADVITLDIEMPGMSGLEFLT
RLMRARPMPVVMLSSLTSKGSDAAVQALSRGAIDCILKPTDGYDLDLSRDICERVYQAACTRPASLKRGEATEKPADAPM
MQRASALGQPCRRGSLILIGASTGGVSALEAVLPKLDPDGAPVVIVQHMPGNFLESFSQRLDRQLPQNVKLAEEGVPLRR
GDVILAPGIGKHTEVVRRMDGWQCRFVANDPAALHCPSVDTLFLSAVSEAKKVSVAILTGLGKDGAQGMLKLAEAGATTF
GQDEETSVVYGMPRVAFGLGAVQHQLPIHKIGEALRESKNASARTARRTTKVPLQ

Sequences:

>Translated_375_residues
MHSDTNEHQSHVPAKKVLIVDDSRVIRMWLRTVLASDRRLEVVGEASDAVEARDYIRANPADVITLDIEMPGMSGLEFLT
RLMRARPMPVVMLSSLTSKGSDAAVQALSRGAIDCILKPTDGYDLDLSRDICERVYQAACTRPASLKRGEATEKPADAPM
MQRASALGQPCRRGSLILIGASTGGVSALEAVLPKLDPDGAPVVIVQHMPGNFLESFSQRLDRQLPQNVKLAEEGVPLRR
GDVILAPGIGKHTEVVRRMDGWQCRFVANDPAALHCPSVDTLFLSAVSEAKKVSVAILTGLGKDGAQGMLKLAEAGATTF
GQDEETSVVYGMPRVAFGLGAVQHQLPIHKIGEALRESKNASARTARRTTKVPLQ
>Mature_375_residues
MHSDTNEHQSHVPAKKVLIVDDSRVIRMWLRTVLASDRRLEVVGEASDAVEARDYIRANPADVITLDIEMPGMSGLEFLT
RLMRARPMPVVMLSSLTSKGSDAAVQALSRGAIDCILKPTDGYDLDLSRDICERVYQAACTRPASLKRGEATEKPADAPM
MQRASALGQPCRRGSLILIGASTGGVSALEAVLPKLDPDGAPVVIVQHMPGNFLESFSQRLDRQLPQNVKLAEEGVPLRR
GDVILAPGIGKHTEVVRRMDGWQCRFVANDPAALHCPSVDTLFLSAVSEAKKVSVAILTGLGKDGAQGMLKLAEAGATTF
GQDEETSVVYGMPRVAFGLGAVQHQLPIHKIGEALRESKNASARTARRTTKVPLQ

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=331, Percent_Identity=42.5981873111783, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082052, Length=106, Percent_Identity=38.6792452830189, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_ROSDO (Q167K9)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682520.1
- ProteinModelPortal:   Q167K9
- SMR:   Q167K9
- STRING:   Q167K9
- GeneID:   4196512
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2244
- NMPDR:   fig|375451.6.peg.2094
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- PhylomeDB:   Q167K9
- BioCyc:   RDEN375451:RD1_2244-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 40425; Mature: 40425

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY; PS01039 SBP_BACTERIAL_3

Important sites: ACT_SITE 182-182 ACT_SITE 208-208 ACT_SITE 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSDTNEHQSHVPAKKVLIVDDSRVIRMWLRTVLASDRRLEVVGEASDAVEARDYIRANP
CCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
ADVITLDIEMPGMSGLEFLTRLMRARPMPVVMLSSLTSKGSDAAVQALSRGAIDCILKPT
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHCCCEEEEEECC
DGYDLDLSRDICERVYQAACTRPASLKRGEATEKPADAPMMQRASALGQPCRRGSLILIG
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
ASTGGVSALEAVLPKLDPDGAPVVIVQHMPGNFLESFSQRLDRQLPQNVKLAEEGVPLRR
CCCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEC
GDVILAPGIGKHTEVVRRMDGWQCRFVANDPAALHCPSVDTLFLSAVSEAKKVSVAILTG
CCEEEECCCCHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHEEEEEEEC
LGKDGAQGMLKLAEAGATTFGQDEETSVVYGMPRVAFGLGAVQHQLPIHKIGEALRESKN
CCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
ASARTARRTTKVPLQ
CCHHHHHHCCCCCCC
>Mature Secondary Structure
MHSDTNEHQSHVPAKKVLIVDDSRVIRMWLRTVLASDRRLEVVGEASDAVEARDYIRANP
CCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
ADVITLDIEMPGMSGLEFLTRLMRARPMPVVMLSSLTSKGSDAAVQALSRGAIDCILKPT
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHCCCEEEEEECC
DGYDLDLSRDICERVYQAACTRPASLKRGEATEKPADAPMMQRASALGQPCRRGSLILIG
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
ASTGGVSALEAVLPKLDPDGAPVVIVQHMPGNFLESFSQRLDRQLPQNVKLAEEGVPLRR
CCCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEC
GDVILAPGIGKHTEVVRRMDGWQCRFVANDPAALHCPSVDTLFLSAVSEAKKVSVAILTG
CCEEEECCCCHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHEEEEEEEC
LGKDGAQGMLKLAEAGATTFGQDEETSVVYGMPRVAFGLGAVQHQLPIHKIGEALRESKN
CCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
ASARTARRTTKVPLQ
CCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA