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The map label for this gene is hsdR [H]

Identifier: 110679504

GI number: 110679504

Start: 2137358

End: 2139706

Strand: Direct

Name: hsdR [H]

Synonym: RD1_2235

Alternate gene names: 110679504

Gene position: 2137358-2139706 (Clockwise)

Preceding gene: 110679503

Following gene: 110679506

Centisome position: 51.71

GC content: 56.24

Gene sequence:

>2349_bases
ATGGTTGAACATTCTTCCCTTGCCACAGAGACCGAAGCGGACACGCGCGCTCAGCGGATAGACCCTGCGCTTGCCGCCGC
CGGATGGGAAGGCGCGGGAAAGATCGACGGCGCGCGGGTGCATCGCGAGGAAATGCTCTGGCCCGGTCGCATCATGTCGG
GCGGTGGGCAGAACGCGCCCAAACCCGCTGACTACGTGCTGCGCATGAAGGGCCACGTTATGGCTGTGTTGGAGGCCAAG
AAAGCCTCGCTATCCTATACTGAAGGGCGCGGCCAAGCCCTCGACTATGCCCGCAGGATCGGCGCGCGCTTTGCCTATTG
CACTAATGGACTGCGCACCTACGAGATTGACCTCAACACGGGCACCGAAGGCGAAGTTGAGGCAGTCCCATCGCCCGACG
TGCTCTGGGAGCGGTGCTATCCCACCGGGAACGCATGGCGCGACCGTTTCGGTCAGATCCCTTTTGAGACTGATGGCGGC
AAATGGCAGCCCCGCTACTATCAGGCGGCGGCTGTGAATGCCGTACTGGAAGCGTTGGCGCAAGACGAAAAGCGCATCCT
ATTGACCCTCGCCACTGGCACCGGCAAAACTTCCATCGCCTTCCAAATCGCATGGAAGTTGTTTCAATCGCGGTGGAACC
TTTCTGGCGCGCCCACACGTCGCCCGCGCATCCTGTTCCTTGCTGACCGCAATATCCTCGCGGATCAGGCGTACAATTCA
TTCTCCGCTTTCGCGCCTGATGCACTGTCGCGGATCAATCCAAAAGAGATCAGCAAGAAGGGCAAGATGCCCCGCAATGC
GAGTGTCTTCTTCACGATCTTCCAGACCTTCATGACGGAAAACAAATCTGGTGAGGCAGAGTTTAACTTTCGCGAATACG
AGCCTGATTTCTTTGACTTCATCATCATTGACGAATGTCACCGGGGCGGTGCGAAGGACGAAAGCTCTTGGCGCGGTATT
CTAGAGTATTTCGCACCTGCTGTGCAGCTTGGTTTGACAGCGACGCCCAAGCGCAAGGTGAACGCCGACACCTACACCTA
TTTCGGCGAACCGGTGTATACATACGCCCTGCGCGAAGGCATTGATGACGGCTTCCTCACGCCCTTCAAAGTGCGCCAGA
TGGCCAGCACGATTGATACCTATGTCTATGACCCGGAGGATGAGGTCGTCGGTGGCGACGTGGACCCCGATCATGAATAC
ACAGAAGATGAGATCAACGCGAAAATCGTCATTCCTGCGCGCGAGCAAAGTCGCATTCATGAATTCATGGATCAGATCAA
CCCACGCCAGAAGACGCTGGTGTTCTGTGCAACCCAGAACCACGCGGCGGCAGTGTGCGACTACATCAACCAGATCAAGG
ACATCGAAGACCCGCACTATTGCGAGCGCGTAACGGCGAATGACGGCGCTCTGGGCGAGCAACACTTGCGCGAGTTTCAG
GACAATGAGCGGAGCATTCCAACAATTCTGACGACATCGCAGAAGCTTTCCACCGGGGTAGATGCGCGCAACGTCCGGAA
CATCGTCCTAATGCGGCCTATCAAGTCGATGGTGGAGTTCAAGCAGATCGTTGGGCGCGGAACACGTACCTTCGAAGGCA
AAGACTTCTTCACCATCTACGACTTCGTGAAAGCATATGAGCATTTCAACGATCCGGAATGGGATGGCCCACCCGAACCG
CCGGACACTCCGGGAACGCCACCGAAACCGCCGAAACCGGGCGACCCTGTGCCCCCTGGCGATCCACCACCGACCCCGCC
GGAACCGGAAGAGAAAATCGTGATCAAGCTTGCTGACGGCAAGGAACGTCGGATCAAGTACATCGCGACAACGAGTTACT
ATTCACACGACGGCAAGATGATCTCCGGTGAAGAGTTCATGGAACAGCTTTTCGGGGATCTAGGCGATCTTGTGACCGGC
GAAGAAGAACTGCGGTTAATCTGGAGCGATCCGGAAAAGCGTGTGCAGTTTCTGAAGGTGTTGGCAGACAGAGGCTACGA
CACTGGGCGGCTTGAGGATATGAAGCGCCTCATTGACGCTCCGAACAGCGATGTGTTCGACGTTCTGGCCTATGTCCGTT
TCACTCTTTCGCCAAAGTTGAGATCAGAAAGAGCAGCGGCGGCGCGTGAGAATGGCTTGACTGGCCTCGAAAGTGAAATG
AAGGCATTTCTTGAAGCGGTGCTTAACGCCTATGAAGTTCACGGGGTCGAAGAGCTTGCCTTGCCAAAGATCAGTGATTT
CCTGAAGGTGAAGTATGGTGGCACGAACGGAGCAAAGCGCGTACTTGGCGACATACCGAAAATCAGGCGCGCGTTCACAG
AAATTCAGGCCCATATCTACTCCAGTTAG

Upstream 100 bases:

>100_bases
AACCAACTACCGAACCAAACTCACTGACATTGCCGACCTGCGTCAATCCCTCCTGCAAAAAGCCTTTGCCGGGGAACTCA
CCTGACGCTAACCTCCAACA

Downstream 100 bases:

>100_bases
ACCTTAGAAATGCTATTTTTTTGTATGTCTGGGGGTATGATTGGGGGTATTGTCAAATCTCATTAGTTTTTCTCATATTT
ATAACAATGATTTAATCGTT

Product: type I restriction enzyme subunit, putative

Products: NA

Alternate protein names: R.EcoAI [H]

Number of amino acids: Translated: 782; Mature: 782

Protein sequence:

>782_residues
MVEHSSLATETEADTRAQRIDPALAAAGWEGAGKIDGARVHREEMLWPGRIMSGGGQNAPKPADYVLRMKGHVMAVLEAK
KASLSYTEGRGQALDYARRIGARFAYCTNGLRTYEIDLNTGTEGEVEAVPSPDVLWERCYPTGNAWRDRFGQIPFETDGG
KWQPRYYQAAAVNAVLEALAQDEKRILLTLATGTGKTSIAFQIAWKLFQSRWNLSGAPTRRPRILFLADRNILADQAYNS
FSAFAPDALSRINPKEISKKGKMPRNASVFFTIFQTFMTENKSGEAEFNFREYEPDFFDFIIIDECHRGGAKDESSWRGI
LEYFAPAVQLGLTATPKRKVNADTYTYFGEPVYTYALREGIDDGFLTPFKVRQMASTIDTYVYDPEDEVVGGDVDPDHEY
TEDEINAKIVIPAREQSRIHEFMDQINPRQKTLVFCATQNHAAAVCDYINQIKDIEDPHYCERVTANDGALGEQHLREFQ
DNERSIPTILTTSQKLSTGVDARNVRNIVLMRPIKSMVEFKQIVGRGTRTFEGKDFFTIYDFVKAYEHFNDPEWDGPPEP
PDTPGTPPKPPKPGDPVPPGDPPPTPPEPEEKIVIKLADGKERRIKYIATTSYYSHDGKMISGEEFMEQLFGDLGDLVTG
EEELRLIWSDPEKRVQFLKVLADRGYDTGRLEDMKRLIDAPNSDVFDVLAYVRFTLSPKLRSERAAAARENGLTGLESEM
KAFLEAVLNAYEVHGVEELALPKISDFLKVKYGGTNGAKRVLGDIPKIRRAFTEIQAHIYSS

Sequences:

>Translated_782_residues
MVEHSSLATETEADTRAQRIDPALAAAGWEGAGKIDGARVHREEMLWPGRIMSGGGQNAPKPADYVLRMKGHVMAVLEAK
KASLSYTEGRGQALDYARRIGARFAYCTNGLRTYEIDLNTGTEGEVEAVPSPDVLWERCYPTGNAWRDRFGQIPFETDGG
KWQPRYYQAAAVNAVLEALAQDEKRILLTLATGTGKTSIAFQIAWKLFQSRWNLSGAPTRRPRILFLADRNILADQAYNS
FSAFAPDALSRINPKEISKKGKMPRNASVFFTIFQTFMTENKSGEAEFNFREYEPDFFDFIIIDECHRGGAKDESSWRGI
LEYFAPAVQLGLTATPKRKVNADTYTYFGEPVYTYALREGIDDGFLTPFKVRQMASTIDTYVYDPEDEVVGGDVDPDHEY
TEDEINAKIVIPAREQSRIHEFMDQINPRQKTLVFCATQNHAAAVCDYINQIKDIEDPHYCERVTANDGALGEQHLREFQ
DNERSIPTILTTSQKLSTGVDARNVRNIVLMRPIKSMVEFKQIVGRGTRTFEGKDFFTIYDFVKAYEHFNDPEWDGPPEP
PDTPGTPPKPPKPGDPVPPGDPPPTPPEPEEKIVIKLADGKERRIKYIATTSYYSHDGKMISGEEFMEQLFGDLGDLVTG
EEELRLIWSDPEKRVQFLKVLADRGYDTGRLEDMKRLIDAPNSDVFDVLAYVRFTLSPKLRSERAAAARENGLTGLESEM
KAFLEAVLNAYEVHGVEELALPKISDFLKVKYGGTNGAKRVLGDIPKIRRAFTEIQAHIYSS
>Mature_782_residues
MVEHSSLATETEADTRAQRIDPALAAAGWEGAGKIDGARVHREEMLWPGRIMSGGGQNAPKPADYVLRMKGHVMAVLEAK
KASLSYTEGRGQALDYARRIGARFAYCTNGLRTYEIDLNTGTEGEVEAVPSPDVLWERCYPTGNAWRDRFGQIPFETDGG
KWQPRYYQAAAVNAVLEALAQDEKRILLTLATGTGKTSIAFQIAWKLFQSRWNLSGAPTRRPRILFLADRNILADQAYNS
FSAFAPDALSRINPKEISKKGKMPRNASVFFTIFQTFMTENKSGEAEFNFREYEPDFFDFIIIDECHRGGAKDESSWRGI
LEYFAPAVQLGLTATPKRKVNADTYTYFGEPVYTYALREGIDDGFLTPFKVRQMASTIDTYVYDPEDEVVGGDVDPDHEY
TEDEINAKIVIPAREQSRIHEFMDQINPRQKTLVFCATQNHAAAVCDYINQIKDIEDPHYCERVTANDGALGEQHLREFQ
DNERSIPTILTTSQKLSTGVDARNVRNIVLMRPIKSMVEFKQIVGRGTRTFEGKDFFTIYDFVKAYEHFNDPEWDGPPEP
PDTPGTPPKPPKPGDPVPPGDPPPTPPEPEEKIVIKLADGKERRIKYIATTSYYSHDGKMISGEEFMEQLFGDLGDLVTG
EEELRLIWSDPEKRVQFLKVLADRGYDTGRLEDMKRLIDAPNSDVFDVLAYVRFTLSPKLRSERAAAARENGLTGLESEM
KAFLEAVLNAYEVHGVEELALPKISDFLKVKYGGTNGAKRVLGDIPKIRRAFTEIQAHIYSS

Specific function: The EcoAI enzyme recognizes 5'-GAGN(7)GTCA-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=631, Percent_Identity=27.4167987321712, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320539, Length=401, Percent_Identity=25.4364089775561, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 87950; Mature: 87950

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEHSSLATETEADTRAQRIDPALAAAGWEGAGKIDGARVHREEMLWPGRIMSGGGQNAP
CCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEECCCCCCCC
KPADYVLRMKGHVMAVLEAKKASLSYTEGRGQALDYARRIGARFAYCTNGLRTYEIDLNT
CCHHHHHHHCCCEEEEEEHHHHCCEECCCCCHHHHHHHHHHCHHHHHCCCCEEEEEEECC
GTEGEVEAVPSPDVLWERCYPTGNAWRDRFGQIPFETDGGKWQPRYYQAAAVNAVLEALA
CCCCCEEECCCCHHHHHHCCCCCCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
QDEKRILLTLATGTGKTSIAFQIAWKLFQSRWNLSGAPTRRPRILFLADRNILADQAYNS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHCC
FSAFAPDALSRINPKEISKKGKMPRNASVFFTIFQTFMTENKSGEAEFNFREYEPDFFDF
HHHCCCHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEECEECCCCCEEEE
IIIDECHRGGAKDESSWRGILEYFAPAVQLGLTATPKRKVNADTYTYFGEPVYTYALREG
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHC
IDDGFLTPFKVRQMASTIDTYVYDPEDEVVGGDVDPDHEYTEDEINAKIVIPAREQSRIH
CCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHH
EFMDQINPRQKTLVFCATQNHAAAVCDYINQIKDIEDPHYCERVTANDGALGEQHLREFQ
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHH
DNERSIPTILTTSQKLSTGVDARNVRNIVLMRPIKSMVEFKQIVGRGTRTFEGKDFFTIY
CCCCCCCEEEECCHHHHCCCCHHHHHHHEEHHHHHHHHHHHHHHCCCCCEECCCCHHHHH
DFVKAYEHFNDPEWDGPPEPPDTPGTPPKPPKPGDPVPPGDPPPTPPEPEEKIVIKLADG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
KERRIKYIATTSYYSHDGKMISGEEFMEQLFGDLGDLVTGEEELRLIWSDPEKRVQFLKV
CCCEEEEEEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
LADRGYDTGRLEDMKRLIDAPNSDVFDVLAYVRFTLSPKLRSERAAAARENGLTGLESEM
HHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHH
KAFLEAVLNAYEVHGVEELALPKISDFLKVKYGGTNGAKRVLGDIPKIRRAFTEIQAHIY
HHHHHHHHHHHHHCCHHHHHCCHHCCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHC
SS
CC
>Mature Secondary Structure
MVEHSSLATETEADTRAQRIDPALAAAGWEGAGKIDGARVHREEMLWPGRIMSGGGQNAP
CCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEECCCCCCCC
KPADYVLRMKGHVMAVLEAKKASLSYTEGRGQALDYARRIGARFAYCTNGLRTYEIDLNT
CCHHHHHHHCCCEEEEEEHHHHCCEECCCCCHHHHHHHHHHCHHHHHCCCCEEEEEEECC
GTEGEVEAVPSPDVLWERCYPTGNAWRDRFGQIPFETDGGKWQPRYYQAAAVNAVLEALA
CCCCCEEECCCCHHHHHHCCCCCCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
QDEKRILLTLATGTGKTSIAFQIAWKLFQSRWNLSGAPTRRPRILFLADRNILADQAYNS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHCC
FSAFAPDALSRINPKEISKKGKMPRNASVFFTIFQTFMTENKSGEAEFNFREYEPDFFDF
HHHCCCHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEECEECCCCCEEEE
IIIDECHRGGAKDESSWRGILEYFAPAVQLGLTATPKRKVNADTYTYFGEPVYTYALREG
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHC
IDDGFLTPFKVRQMASTIDTYVYDPEDEVVGGDVDPDHEYTEDEINAKIVIPAREQSRIH
CCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHH
EFMDQINPRQKTLVFCATQNHAAAVCDYINQIKDIEDPHYCERVTANDGALGEQHLREFQ
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHH
DNERSIPTILTTSQKLSTGVDARNVRNIVLMRPIKSMVEFKQIVGRGTRTFEGKDFFTIY
CCCCCCCEEEECCHHHHCCCCHHHHHHHEEHHHHHHHHHHHHHHCCCCCEECCCCHHHHH
DFVKAYEHFNDPEWDGPPEPPDTPGTPPKPPKPGDPVPPGDPPPTPPEPEEKIVIKLADG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
KERRIKYIATTSYYSHDGKMISGEEFMEQLFGDLGDLVTGEEELRLIWSDPEKRVQFLKV
CCCEEEEEEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
LADRGYDTGRLEDMKRLIDAPNSDVFDVLAYVRFTLSPKLRSERAAAARENGLTGLESEM
HHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHH
KAFLEAVLNAYEVHGVEELALPKISDFLKVKYGGTNGAKRVLGDIPKIRRAFTEIQAHIY
HHHHHHHHHHHHHCCHHHHHCCHHCCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHC
SS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]