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The map label for this gene is hsdM [H]

Identifier: 110679502

GI number: 110679502

Start: 2134764

End: 2136206

Strand: Direct

Name: hsdM [H]

Synonym: RD1_2233

Alternate gene names: 110679502

Gene position: 2134764-2136206 (Clockwise)

Preceding gene: 110679501

Following gene: 110679503

Centisome position: 51.65

GC content: 57.45

Gene sequence:

>1443_bases
ATGTTTGAGAACGCTTTCAACAGCATAGACAAGGAACTGCGCAACGAAGACGGATTGGCGTCGGAGTTGGACTACGCCGA
ACAGACCTCTTGGATCCTCTTTCTCAAGTATCTGGACGATATGGAGCAGGAGCGCGAAGACGAAGCGGAACTCGAAGGCA
AGGACTACACGCCAACCCTTCCGAACGACATGCGTTGGAAATCCTGGGCCTCACCCAAGAATCCTGACGGAACCGACCGC
AAGGACGTTCTGACTGGCGAAGACCTCATCACCTTCGTCAACACCGAACTCTTCCCGACGCTCAAGAAGTACCGCGAGGA
CGCCACAAGCCCCGATACCATCGAATACAAGATCGGCGAGATCTTTTCCGAGATCACCAACAAGTTCCGCTCCGGCTATT
CCCTGCGGGACGTGCTTGAAATCGTGGACGGGCTGGAGTTCAACACGCAGGAAGCCAAACATGAGCTTTCCGACCTTTAC
GAGTCCCGCATCAAACGCATGGGCAACGCAGGGCGCAACGGCGGCGAATACTACACGCCAAGGCCCCTTATCCGCGCCAT
GATCAAGGTGGTGGACCCCAAGATCGGCGAGACGGTCTATGACGGCGCGTGCGGCTCTGCGGGCTTTCTCTGCGAAGCCT
ACGCGCATATGCTGACGACCGACATCTCGGCCAGCGATTACAGCACCCTGCAAACGCGCACCTTCTACGGGCAGGAGAAA
AAGTCGCTCGCCTATATCATCGGCATTATGAATATGATCCTTCACGGAATTACCGCGCCCAACATCCGGCGCACCAATAC
GCTCACCGAAAACGTCATGGATATTCAGGAAAAGGACCGACACGACGTGATCCTTGCCAATCCGCCTTTTGGCTCTGGCG
AGCGTCCCGAGGTACAGCAGAACTTCCCGATCAAGTCGGGCGAGATGGCGTATCTGTTCATGCAGCACTTCATCCGCAAA
CTGCGGGCGGGTGGGCGCGCGGCGGTTGTCATCAAGAACACCTTTCTAAGCAATGGCGATGCGGCGGCCTTGCGGCGTGA
GTTGCTGGAGACTTGTGACCTGCACACGGTGCTCGACTGCCCCGCCAAGGTGTTTCAGGGCGCGGGCGTGAAAACCGTCG
TGCTGTTTTTCGAGAAAGGGCGCAAAACGCGCAAAACATGGTACTACGCGCTCGATCCGGGGCGGTCATTGGGCAAAGGC
TCTCCGCTGCGGGATGATGAGCTGGCGGAGTTTCTGGAGCTTCAAAAGACAAAGGCCGACAGCCCCAAAAGCTGGACCGT
CGCGCGCGAGGACATCGATGCGGAGACGATGGATATGTCTGTGAAGAACCCCTTCGCCCCCGAAGAAGCGCCGCTCCGCT
CGCCCGAAGAGATCATCGAAGATATGCTGGCCCGAGACAAGGAGACAGCGGCAATACTGGAAGACATCCGGGGGATGTTG
TGA

Upstream 100 bases:

>100_bases
GGAACGACAAGGCGAACTCATCAAAGCACCCAAAATTCCGGCTTAGGCCGTTGTCCATTCCGCGCCCAGCGCCTACGGTA
ATCAAAAAACAGGGCAAAAC

Downstream 100 bases:

>100_bases
AAGCAGGTTGGGAGGTTAAGCCGCTTGGCGAAGTTGCAAAATTGCACTATGGAAAAGCCCTTGCAGAATCTGAGCGTAGT
CCAAACGGCACTGTGCCAGT

Product: type I restriction enzyme methyltransferase subunit, putative

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MFENAFNSIDKELRNEDGLASELDYAEQTSWILFLKYLDDMEQEREDEAELEGKDYTPTLPNDMRWKSWASPKNPDGTDR
KDVLTGEDLITFVNTELFPTLKKYREDATSPDTIEYKIGEIFSEITNKFRSGYSLRDVLEIVDGLEFNTQEAKHELSDLY
ESRIKRMGNAGRNGGEYYTPRPLIRAMIKVVDPKIGETVYDGACGSAGFLCEAYAHMLTTDISASDYSTLQTRTFYGQEK
KSLAYIIGIMNMILHGITAPNIRRTNTLTENVMDIQEKDRHDVILANPPFGSGERPEVQQNFPIKSGEMAYLFMQHFIRK
LRAGGRAAVVIKNTFLSNGDAAALRRELLETCDLHTVLDCPAKVFQGAGVKTVVLFFEKGRKTRKTWYYALDPGRSLGKG
SPLRDDELAEFLELQKTKADSPKSWTVAREDIDAETMDMSVKNPFAPEEAPLRSPEEIIEDMLARDKETAAILEDIRGML

Sequences:

>Translated_480_residues
MFENAFNSIDKELRNEDGLASELDYAEQTSWILFLKYLDDMEQEREDEAELEGKDYTPTLPNDMRWKSWASPKNPDGTDR
KDVLTGEDLITFVNTELFPTLKKYREDATSPDTIEYKIGEIFSEITNKFRSGYSLRDVLEIVDGLEFNTQEAKHELSDLY
ESRIKRMGNAGRNGGEYYTPRPLIRAMIKVVDPKIGETVYDGACGSAGFLCEAYAHMLTTDISASDYSTLQTRTFYGQEK
KSLAYIIGIMNMILHGITAPNIRRTNTLTENVMDIQEKDRHDVILANPPFGSGERPEVQQNFPIKSGEMAYLFMQHFIRK
LRAGGRAAVVIKNTFLSNGDAAALRRELLETCDLHTVLDCPAKVFQGAGVKTVVLFFEKGRKTRKTWYYALDPGRSLGKG
SPLRDDELAEFLELQKTKADSPKSWTVAREDIDAETMDMSVKNPFAPEEAPLRSPEEIIEDMLARDKETAAILEDIRGML
>Mature_480_residues
MFENAFNSIDKELRNEDGLASELDYAEQTSWILFLKYLDDMEQEREDEAELEGKDYTPTLPNDMRWKSWASPKNPDGTDR
KDVLTGEDLITFVNTELFPTLKKYREDATSPDTIEYKIGEIFSEITNKFRSGYSLRDVLEIVDGLEFNTQEAKHELSDLY
ESRIKRMGNAGRNGGEYYTPRPLIRAMIKVVDPKIGETVYDGACGSAGFLCEAYAHMLTTDISASDYSTLQTRTFYGQEK
KSLAYIIGIMNMILHGITAPNIRRTNTLTENVMDIQEKDRHDVILANPPFGSGERPEVQQNFPIKSGEMAYLFMQHFIRK
LRAGGRAAVVIKNTFLSNGDAAALRRELLETCDLHTVLDCPAKVFQGAGVKTVVLFFEKGRKTRKTWYYALDPGRSLGKG
SPLRDDELAEFLELQKTKADSPKSWTVAREDIDAETMDMSVKNPFAPEEAPLRSPEEIIEDMLARDKETAAILEDIRGML

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=421, Percent_Identity=29.9287410926366, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 54426; Mature: 54426

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFENAFNSIDKELRNEDGLASELDYAEQTSWILFLKYLDDMEQEREDEAELEGKDYTPTL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PNDMRWKSWASPKNPDGTDRKDVLTGEDLITFVNTELFPTLKKYREDATSPDTIEYKIGE
CCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
IFSEITNKFRSGYSLRDVLEIVDGLEFNTQEAKHELSDLYESRIKRMGNAGRNGGEYYTP
HHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPLIRAMIKVVDPKIGETVYDGACGSAGFLCEAYAHMLTTDISASDYSTLQTRTFYGQEK
CHHHHHHHHHHCCHHCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHH
KSLAYIIGIMNMILHGITAPNIRRTNTLTENVMDIQEKDRHDVILANPPFGSGERPEVQQ
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHC
NFPIKSGEMAYLFMQHFIRKLRAGGRAAVVIKNTFLSNGDAAALRRELLETCDLHTVLDC
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHC
PAKVFQGAGVKTVVLFFEKGRKTRKTWYYALDPGRSLGKGSPLRDDELAEFLELQKTKAD
HHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SPKSWTVAREDIDAETMDMSVKNPFAPEEAPLRSPEEIIEDMLARDKETAAILEDIRGML
CCCCCEEEHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFENAFNSIDKELRNEDGLASELDYAEQTSWILFLKYLDDMEQEREDEAELEGKDYTPTL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PNDMRWKSWASPKNPDGTDRKDVLTGEDLITFVNTELFPTLKKYREDATSPDTIEYKIGE
CCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
IFSEITNKFRSGYSLRDVLEIVDGLEFNTQEAKHELSDLYESRIKRMGNAGRNGGEYYTP
HHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPLIRAMIKVVDPKIGETVYDGACGSAGFLCEAYAHMLTTDISASDYSTLQTRTFYGQEK
CHHHHHHHHHHCCHHCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHH
KSLAYIIGIMNMILHGITAPNIRRTNTLTENVMDIQEKDRHDVILANPPFGSGERPEVQQ
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHC
NFPIKSGEMAYLFMQHFIRKLRAGGRAAVVIKNTFLSNGDAAALRRELLETCDLHTVLDC
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHC
PAKVFQGAGVKTVVLFFEKGRKTRKTWYYALDPGRSLGKGSPLRDDELAEFLELQKTKAD
HHHHHCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SPKSWTVAREDIDAETMDMSVKNPFAPEEAPLRSPEEIIEDMLARDKETAAILEDIRGML
CCCCCEEEHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]