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The map label for this gene is gsiB [H]

Identifier: 110679496

GI number: 110679496

Start: 2126507

End: 2128072

Strand: Reverse

Name: gsiB [H]

Synonym: RD1_2225

Alternate gene names: 110679496

Gene position: 2128072-2126507 (Counterclockwise)

Preceding gene: 110679497

Following gene: 110679495

Centisome position: 51.49

GC content: 61.3

Gene sequence:

>1566_bases
ATGTTACGTAATTTCAGCTTTGCCGCCCTCTTTGCCGTTCTGGCACCTGTCACGCAGGCAACAGAATTCACATGGTCCAG
CACGACGGATCCGCAAACCATGGACCCCCATGCGGTCAATTCCGCACCGGTTCTGGGGTTTCTCAACAACGTCTACGAAG
GTCTTGTACGGCGCGGCCCGGACATGGCCATCGAGCCCGCCCTCGCCACAAGCTGGGAAGCGATTGGCGAGGGCGCGGGC
TGGCGTTTCAACCTGCGCCGCGATGTGACCTTTCACGATGGCGCTGAATTCACCGCACAGGACGTGCTGTTTTCCTATGA
GCGCGCCGCCAGCGATGCGGCGGACACCAGCAGTTGGTTCGCGCCGGTCTCGGAGGTGATCCTCGTCGATGATTTCACGG
TCGATTTCATGACCACCGCGCCCAATCCGATCTTTCCCGACAGTATCGCAAACTGGATGATCATGGACAGCGACTGGGCT
GTGGCAAACGATGCCACCCGCCCGGACAAGGAACGCGGCAACCATGCCACGCTGCACGCCAATGGCACCGGGGCCTTTGC
GATCACCAGCCGTGAACCCGGGCTGCGCACCGTGCTTGAACCGCACGCAGGCTGGTGGGGCGCGGCGACCCATAACATCA
CCCGGGCAGAGTTTACGCCCATCCAGAACCCCGCCACCGCCATCGCGGCTTTGCTTTCGGGGGAAGTCGATTTCATCAAC
CCGGTGCCGGTGCAGGATGTCGCGCGCTTGCAGCAGGACGCCAATATCAGGGTGATACAGGGCATCGAGGCGCGTGTGAT
CATGTTGGGCTTTCCCCATGAGGCAGAAGCATTGAAGTATTCTGCGGAAACACCGGACGCCAATCCCTTCACCGACCCGC
GCGTGCGCCGCGCCGTAGCCCATGCCATCAACGTGCCCGCGATCCTGCAAACAACGATGCGCGGCAACGCCGAAGCGGTG
AACCAATTGGTCAGCCCCGCCATGCGCGGCTATTCCGATGCGCTGGCCGGGCAGCCCCCCTATGACCCCGATGCCGCGCG
CGCCCTGTTGGCCGAAGCAGGATATCCGGATGGGTTTTCATTCGGGTTCAAATGCCCCAATGACCGCTACCTCAATGATG
AAGCGGTCTGTCAGGCGATCACGGCCATGCTGTCACAGGTGGGCATGCGCGCCACGCTCGATGCCATGCCGGTGCAGACC
TATTGGCCTGAATTGCGCGCGGACAATTACGACATGTACCTGCTGGGCTGGTCGCCCGGCACCTTTGATGCTGAACACCC
GATCCGCTTTCTGGCCGCGACCCCGAATGCGGAAAAGCGGCTGGGCAGCTGGAATTTCGGCGGCTACTCCAATGCACGCG
TCGATGAATTGCTGCCAATGATCCAGTCGGAAATTGACGAGCCTGCGCGTCAGGCGATGCTGGATGAGGTGGCGCAGATC
CTGCAGCGGGACACGGCCTATGTGCCGCTTTATGTGCAGCCGCTCGTGTGGGGAACCCGTGCGCATATTGATCTGGTGCA
ACGGCCCGACAACTTCTTTATTCTGCGCTGGGTTAGCCTTAACTAG

Upstream 100 bases:

>100_bases
CACCATGAACTAAACGAGACGCGGTGCGCCCTGCCCTTGGGCGCACCGCTGTTTTCATGCGTCAGCACAGGCCACCGCTT
TGGCCCGGTTCAGGGATAGA

Downstream 100 bases:

>100_bases
GAACATCTGCGGGGCGTTCAGGCCCTGCGGCAACGGGGATAGTCACCATCATGCTTGCCTTTATCATCCGGCGTGTCCTG
CAATCCGCGCTTGTCCTGCT

Product: solute-binding family 5 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MLRNFSFAALFAVLAPVTQATEFTWSSTTDPQTMDPHAVNSAPVLGFLNNVYEGLVRRGPDMAIEPALATSWEAIGEGAG
WRFNLRRDVTFHDGAEFTAQDVLFSYERAASDAADTSSWFAPVSEVILVDDFTVDFMTTAPNPIFPDSIANWMIMDSDWA
VANDATRPDKERGNHATLHANGTGAFAITSREPGLRTVLEPHAGWWGAATHNITRAEFTPIQNPATAIAALLSGEVDFIN
PVPVQDVARLQQDANIRVIQGIEARVIMLGFPHEAEALKYSAETPDANPFTDPRVRRAVAHAINVPAILQTTMRGNAEAV
NQLVSPAMRGYSDALAGQPPYDPDAARALLAEAGYPDGFSFGFKCPNDRYLNDEAVCQAITAMLSQVGMRATLDAMPVQT
YWPELRADNYDMYLLGWSPGTFDAEHPIRFLAATPNAEKRLGSWNFGGYSNARVDELLPMIQSEIDEPARQAMLDEVAQI
LQRDTAYVPLYVQPLVWGTRAHIDLVQRPDNFFILRWVSLN

Sequences:

>Translated_521_residues
MLRNFSFAALFAVLAPVTQATEFTWSSTTDPQTMDPHAVNSAPVLGFLNNVYEGLVRRGPDMAIEPALATSWEAIGEGAG
WRFNLRRDVTFHDGAEFTAQDVLFSYERAASDAADTSSWFAPVSEVILVDDFTVDFMTTAPNPIFPDSIANWMIMDSDWA
VANDATRPDKERGNHATLHANGTGAFAITSREPGLRTVLEPHAGWWGAATHNITRAEFTPIQNPATAIAALLSGEVDFIN
PVPVQDVARLQQDANIRVIQGIEARVIMLGFPHEAEALKYSAETPDANPFTDPRVRRAVAHAINVPAILQTTMRGNAEAV
NQLVSPAMRGYSDALAGQPPYDPDAARALLAEAGYPDGFSFGFKCPNDRYLNDEAVCQAITAMLSQVGMRATLDAMPVQT
YWPELRADNYDMYLLGWSPGTFDAEHPIRFLAATPNAEKRLGSWNFGGYSNARVDELLPMIQSEIDEPARQAMLDEVAQI
LQRDTAYVPLYVQPLVWGTRAHIDLVQRPDNFFILRWVSLN
>Mature_521_residues
MLRNFSFAALFAVLAPVTQATEFTWSSTTDPQTMDPHAVNSAPVLGFLNNVYEGLVRRGPDMAIEPALATSWEAIGEGAG
WRFNLRRDVTFHDGAEFTAQDVLFSYERAASDAADTSSWFAPVSEVILVDDFTVDFMTTAPNPIFPDSIANWMIMDSDWA
VANDATRPDKERGNHATLHANGTGAFAITSREPGLRTVLEPHAGWWGAATHNITRAEFTPIQNPATAIAALLSGEVDFIN
PVPVQDVARLQQDANIRVIQGIEARVIMLGFPHEAEALKYSAETPDANPFTDPRVRRAVAHAINVPAILQTTMRGNAEAV
NQLVSPAMRGYSDALAGQPPYDPDAARALLAEAGYPDGFSFGFKCPNDRYLNDEAVCQAITAMLSQVGMRATLDAMPVQT
YWPELRADNYDMYLLGWSPGTFDAEHPIRFLAATPNAEKRLGSWNFGGYSNARVDELLPMIQSEIDEPARQAMLDEVAQI
LQRDTAYVPLYVQPLVWGTRAHIDLVQRPDNFFILRWVSLN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=475, Percent_Identity=26.1052631578947, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI1789966, Length=506, Percent_Identity=26.0869565217391, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789397, Length=523, Percent_Identity=24.282982791587, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787762, Length=506, Percent_Identity=25.296442687747, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787495, Length=540, Percent_Identity=22.962962962963, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081878, Length=522, Percent_Identity=22.6053639846743, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1789887, Length=522, Percent_Identity=21.8390804597701, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787551, Length=497, Percent_Identity=22.9376257545272, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57389; Mature: 57389

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRNFSFAALFAVLAPVTQATEFTWSSTTDPQTMDPHAVNSAPVLGFLNNVYEGLVRRGP
CCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DMAIEPALATSWEAIGEGAGWRFNLRRDVTFHDGAEFTAQDVLFSYERAASDAADTSSWF
CCEECCHHHHHHHHHCCCCCEEEEEEECCEECCCCCHHHHHHHHHHHHHHCCCCCCHHHH
APVSEVILVDDFTVDFMTTAPNPIFPDSIANWMIMDSDWAVANDATRPDKERGNHATLHA
HCCCCEEEEECCEEEEEECCCCCCCCHHHCCEEEECCCCCCCCCCCCCHHHCCCEEEEEE
NGTGAFAITSREPGLRTVLEPHAGWWGAATHNITRAEFTPIQNPATAIAALLSGEVDFIN
CCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PVPVQDVARLQQDANIRVIQGIEARVIMLGFPHEAEALKYSAETPDANPFTDPRVRRAVA
CCCHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHEECCCCCCCCCCCCHHHHHHHH
HAINVPAILQTTMRGNAEAVNQLVSPAMRGYSDALAGQPPYDPDAARALLAEAGYPDGFS
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
FGFKCPNDRYLNDEAVCQAITAMLSQVGMRATLDAMPVQTYWPELRADNYDMYLLGWSPG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHCHHHCCCCCCEEEEECCCC
TFDAEHPIRFLAATPNAEKRLGSWNFGGYSNARVDELLPMIQSEIDEPARQAMLDEVAQI
CCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
LQRDTAYVPLYVQPLVWGTRAHIDLVQRPDNFFILRWVSLN
HHCCCCEEEEEEECEECCCCCEEEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MLRNFSFAALFAVLAPVTQATEFTWSSTTDPQTMDPHAVNSAPVLGFLNNVYEGLVRRGP
CCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DMAIEPALATSWEAIGEGAGWRFNLRRDVTFHDGAEFTAQDVLFSYERAASDAADTSSWF
CCEECCHHHHHHHHHCCCCCEEEEEEECCEECCCCCHHHHHHHHHHHHHHCCCCCCHHHH
APVSEVILVDDFTVDFMTTAPNPIFPDSIANWMIMDSDWAVANDATRPDKERGNHATLHA
HCCCCEEEEECCEEEEEECCCCCCCCHHHCCEEEECCCCCCCCCCCCCHHHCCCEEEEEE
NGTGAFAITSREPGLRTVLEPHAGWWGAATHNITRAEFTPIQNPATAIAALLSGEVDFIN
CCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PVPVQDVARLQQDANIRVIQGIEARVIMLGFPHEAEALKYSAETPDANPFTDPRVRRAVA
CCCHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHEECCCCCCCCCCCCHHHHHHHH
HAINVPAILQTTMRGNAEAVNQLVSPAMRGYSDALAGQPPYDPDAARALLAEAGYPDGFS
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
FGFKCPNDRYLNDEAVCQAITAMLSQVGMRATLDAMPVQTYWPELRADNYDMYLLGWSPG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHCHHHCCCCCCEEEEECCCC
TFDAEHPIRFLAATPNAEKRLGSWNFGGYSNARVDELLPMIQSEIDEPARQAMLDEVAQI
CCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
LQRDTAYVPLYVQPLVWGTRAHIDLVQRPDNFFILRWVSLN
HHCCCCEEEEEEECEECCCCCEEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA