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The map label for this gene is truA

Identifier: 110679351

GI number: 110679351

Start: 1976973

End: 1977755

Strand: Reverse

Name: truA

Synonym: RD1_2070

Alternate gene names: 110679351

Gene position: 1977755-1976973 (Counterclockwise)

Preceding gene: 110679358

Following gene: 110679345

Centisome position: 47.85

GC content: 62.96

Gene sequence:

>783_bases
ATGCACAGATACGCCCTCAAAGTCGAATATAACGGTGCGCCCTTTGTCGGATGGCAGCGTCAAAAAGAACACCCCACCAT
TCAGGGGGCGATTGAGCGCGCGTTGGGCAAGATCGCGCCGGGCCCGCACACGATCGCGGCTGCAGGGCGCACGGATGCGG
GTGTGCATGCGATCGCGCAGGTGGCGCATTGTGATCTTGAGCGCGACTGGGACCCCTTTCGTCTGTCGGAAGCGTTGAAT
TACCACCTCAAACCCGCGCCCATCGCCATCACCGCCTGCACAGCCGTGGACGCGGAGTGGCACGCAAGGTTTTCCGCGCG
CCAACGTCAGTATCTGTTTCGCATCCTGTGCCGCCGCGCCCCGGCCACCCACCAAAAAGGGTTGGTGTGGCAGGTGAAAC
AGAAGCTTGATGTAACCGCCATGCGCGAAGCCGCGGCGCTGCTGATCGGCCAGCATGATTTCACGACATTCCGCTCCACC
ATCTGTCAGGCGGAAAGCCCGGTCAAAACGCTCGACCGGCTGGATGTGAACGAAGTCGATACGCCCTATGGCCGTGAGAT
TCATTTCGACGTGCGCGCCAGATCCTTCCTGCACAATCAGGTGCGCAGCTTCGTGGGCACGCTGGAACGGGTTGGGGCCG
GGTCGTCCACACCGCAGGATGTGGCAAGGGCGCTGGCCTTGCGGGACCGCGCCGCCTGCGGGCCGGTTTGCCCGCCCCAT
GGCCTCTATCTGGCGCATGTGGTTTACGACCGCCCGCCCTTTGAGGCGGAGCGATCGATCTAA

Upstream 100 bases:

>100_bases
TTGAACTTCTCGATTGGCCCTACATAAGCGCGCATCCCCGAGCACGCCAGAGCAAGAAAGCCATTTGACGCTGGCGGGCG
AGTTGGCTAGGCCCACGCCC

Downstream 100 bases:

>100_bases
TCGTTATCCGTCAGCCCGGCACCGGCACCGGCACTGCGTGAGGCGGCACTTTCGGGCACAAAGGTGCGCGCGGCCAGATC
GCTCATCCGCGCGACCAGCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MHRYALKVEYNGAPFVGWQRQKEHPTIQGAIERALGKIAPGPHTIAAAGRTDAGVHAIAQVAHCDLERDWDPFRLSEALN
YHLKPAPIAITACTAVDAEWHARFSARQRQYLFRILCRRAPATHQKGLVWQVKQKLDVTAMREAAALLIGQHDFTTFRST
ICQAESPVKTLDRLDVNEVDTPYGREIHFDVRARSFLHNQVRSFVGTLERVGAGSSTPQDVARALALRDRAACGPVCPPH
GLYLAHVVYDRPPFEAERSI

Sequences:

>Translated_260_residues
MHRYALKVEYNGAPFVGWQRQKEHPTIQGAIERALGKIAPGPHTIAAAGRTDAGVHAIAQVAHCDLERDWDPFRLSEALN
YHLKPAPIAITACTAVDAEWHARFSARQRQYLFRILCRRAPATHQKGLVWQVKQKLDVTAMREAAALLIGQHDFTTFRST
ICQAESPVKTLDRLDVNEVDTPYGREIHFDVRARSFLHNQVRSFVGTLERVGAGSSTPQDVARALALRDRAACGPVCPPH
GLYLAHVVYDRPPFEAERSI
>Mature_260_residues
MHRYALKVEYNGAPFVGWQRQKEHPTIQGAIERALGKIAPGPHTIAAAGRTDAGVHAIAQVAHCDLERDWDPFRLSEALN
YHLKPAPIAITACTAVDAEWHARFSARQRQYLFRILCRRAPATHQKGLVWQVKQKLDVTAMREAAALLIGQHDFTTFRST
ICQAESPVKTLDRLDVNEVDTPYGREIHFDVRARSFLHNQVRSFVGTLERVGAGSSTPQDVARALALRDRAACGPVCPPH
GLYLAHVVYDRPPFEAERSI

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=33.5766423357664, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=36.4, Blast_Score=153, Evalue=9e-39,
Organism=Drosophila melanogaster, GI116007752, Length=306, Percent_Identity=28.1045751633987, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ROSDO (Q168C1)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682358.1
- ProteinModelPortal:   Q168C1
- SMR:   Q168C1
- STRING:   Q168C1
- GeneID:   4196658
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2070
- NMPDR:   fig|375451.6.peg.1932
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q168C1
- ProtClustDB:   PRK00021
- BioCyc:   RDEN375451:RD1_2070-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29055; Mature: 29055

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYALKVEYNGAPFVGWQRQKEHPTIQGAIERALGKIAPGPHTIAAAGRTDAGVHAIAQ
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHH
VAHCDLERDWDPFRLSEALNYHLKPAPIAITACTAVDAEWHARFSARQRQYLFRILCRRA
HHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
PATHQKGLVWQVKQKLDVTAMREAAALLIGQHDFTTFRSTICQAESPVKTLDRLDVNEVD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCC
TPYGREIHFDVRARSFLHNQVRSFVGTLERVGAGSSTPQDVARALALRDRAACGPVCPPH
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GLYLAHVVYDRPPFEAERSI
CCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MHRYALKVEYNGAPFVGWQRQKEHPTIQGAIERALGKIAPGPHTIAAAGRTDAGVHAIAQ
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHH
VAHCDLERDWDPFRLSEALNYHLKPAPIAITACTAVDAEWHARFSARQRQYLFRILCRRA
HHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
PATHQKGLVWQVKQKLDVTAMREAAALLIGQHDFTTFRSTICQAESPVKTLDRLDVNEVD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCC
TPYGREIHFDVRARSFLHNQVRSFVGTLERVGAGSSTPQDVARALALRDRAACGPVCPPH
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GLYLAHVVYDRPPFEAERSI
CCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA