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The map label for this gene is gatA

Identifier: 110679283

GI number: 110679283

Start: 1908170

End: 1909657

Strand: Reverse

Name: gatA

Synonym: RD1_2000

Alternate gene names: 110679283

Gene position: 1909657-1908170 (Counterclockwise)

Preceding gene: 110679284

Following gene: 110679282

Centisome position: 46.2

GC content: 61.29

Gene sequence:

>1488_bases
ATGAGTAAGCTGAATGAATTGGGACTGGCCGAGGCGCGCGATGCCCTGCGCGCAGGTGAAACTACCTCGAAAGACCTGAC
CGAGGCCTGCCTCACCGCCATCGAAGAAGCGGGCGTGTTGAACGCCTTTGTGCACCACACCGCCGATCTTGCGCTTGAGC
GGGCAGCAGCGGCCGACGCGCGTCTGGCACAAGGGGCGGCCCCTGCGATGTGCGGTCTGCCCATCGGGATGAAAGACCTG
TTCTGCACCAAAGGTGTCGCGTCACAGGCAGGATCGCGCATTCTCGAGGGCTTTTTGCCCGAGTATGAATCCACCGTCAG
CCAGAACCTTGTGGACGCCGGGGCAGTCATGCTGGGCAAGCTCAACATGGATGAATTCGCCATGGGCTCGTCCAACGAAA
CCTCGATCTATGGCGATGTGATCAACCCGTGGCGGCGCAAGGACGATGAAAAACCGTTGACACCGGGGGGCTCTTCGGGC
GGTTCGGCGGCGGCGGTTGCCGCTGATCTGTGCCTTGCCGCGACCGGTACGGACACCGGCGGCTCCATCCGCCAGCCTGC
GGCTTTTACCGGCACCGTAGGCATCAAACCGACCTATGGGCGTTGTTCACGCTGGGGGATTGTGGCCTTTGCCTCCTCAC
TTGATCAGGCGGGACCGATGACGAAATCGGTACGCGATGCTGCCATCATGCTGGAGGCGATGTCAGGGCATGACCCAAAG
GACAGCACCTCTGCCGATCTGGCCGTTCCGGATTTCGAGGCGGCGCTGAGCGGGGATATTCGCGGCAAAAAGATCGGCAT
CCCCAAGGAATACCGCATGGATGGTATGCCCGATGAGATCGAAGCGCTCTGGCAAACCGGCATCGCCATGATGAAGGACG
CAGGCGCTGAAATCATCGACATCTCCCTGCCGCACACGAAATACGCGCTGCCCGCCTATTACGTGATTGCCCCGGCCGAG
GCGTCGTCGAACCTCGCGCGCTATGACGGTGTGCGCTATGGACACCGCGCCAAGCTGGATCAGGGCGATGGCATCACGGA
GATGTATGAAAAGACCCGCGCCGAAGGCTTCGGACCCGAAGTGCAGCGCCGCGTGATGGTCGGCACCTACGTGCTGTCCG
CCGGTTTTTACGACGCCTATTACAACCGCGCGCGCAAGGTCCGCACACTGATCAAGCAGGATTTTGAAACAGTGTTTGCG
CAGGGTGTCGATGCGATATTGACGCCAGCGACCCCGTCGGCGGCCTTTGGTCTGGGCGAAATGACCGACGCCGATCCGGT
CGCGATGTATCTCAATGACATCTTTACCGTGACCGTCAATCTGGCCGGACTACCGGGCATTGCGGTGCCGGGCGGGCTTG
ACGCCAATGGCCTGCCGCTTGGTCTGCAACTCATCGGTCGCCCGTGGGAAGAGGCCGAGTTGCTGAACACCGCCTATGCT
CTTGAGCAGGCAGTGGGTTTTGTGGCCAAGCCGCAGAAATGGTGGTAG

Upstream 100 bases:

>100_bases
GCGCGGATGTTGTTGCGGATGGGGATCAGCAGGCACGTGTCCTGTCCAATGCGCCGGATGCGCGCGAAGGGTTCTTTGCC
GTGCCGAAGGTGGTTGAATA

Downstream 100 bases:

>100_bases
TGTTACGGGATGGAAAAAGTGGGTAAAATGATGGGACGGCGCATGCGACCACATCTCTCCATGGCACTGCCTGTGGCACT
GCTCCTTTCGGCCTGTGCTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSKLNELGLAEARDALRAGETTSKDLTEACLTAIEEAGVLNAFVHHTADLALERAAAADARLAQGAAPAMCGLPIGMKDL
FCTKGVASQAGSRILEGFLPEYESTVSQNLVDAGAVMLGKLNMDEFAMGSSNETSIYGDVINPWRRKDDEKPLTPGGSSG
GSAAAVAADLCLAATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKSVRDAAIMLEAMSGHDPK
DSTSADLAVPDFEAALSGDIRGKKIGIPKEYRMDGMPDEIEALWQTGIAMMKDAGAEIIDISLPHTKYALPAYYVIAPAE
ASSNLARYDGVRYGHRAKLDQGDGITEMYEKTRAEGFGPEVQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKQDFETVFA
QGVDAILTPATPSAAFGLGEMTDADPVAMYLNDIFTVTVNLAGLPGIAVPGGLDANGLPLGLQLIGRPWEEAELLNTAYA
LEQAVGFVAKPQKWW

Sequences:

>Translated_495_residues
MSKLNELGLAEARDALRAGETTSKDLTEACLTAIEEAGVLNAFVHHTADLALERAAAADARLAQGAAPAMCGLPIGMKDL
FCTKGVASQAGSRILEGFLPEYESTVSQNLVDAGAVMLGKLNMDEFAMGSSNETSIYGDVINPWRRKDDEKPLTPGGSSG
GSAAAVAADLCLAATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKSVRDAAIMLEAMSGHDPK
DSTSADLAVPDFEAALSGDIRGKKIGIPKEYRMDGMPDEIEALWQTGIAMMKDAGAEIIDISLPHTKYALPAYYVIAPAE
ASSNLARYDGVRYGHRAKLDQGDGITEMYEKTRAEGFGPEVQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKQDFETVFA
QGVDAILTPATPSAAFGLGEMTDADPVAMYLNDIFTVTVNLAGLPGIAVPGGLDANGLPLGLQLIGRPWEEAELLNTAYA
LEQAVGFVAKPQKWW
>Mature_494_residues
SKLNELGLAEARDALRAGETTSKDLTEACLTAIEEAGVLNAFVHHTADLALERAAAADARLAQGAAPAMCGLPIGMKDLF
CTKGVASQAGSRILEGFLPEYESTVSQNLVDAGAVMLGKLNMDEFAMGSSNETSIYGDVINPWRRKDDEKPLTPGGSSGG
SAAAVAADLCLAATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKSVRDAAIMLEAMSGHDPKD
STSADLAVPDFEAALSGDIRGKKIGIPKEYRMDGMPDEIEALWQTGIAMMKDAGAEIIDISLPHTKYALPAYYVIAPAEA
SSNLARYDGVRYGHRAKLDQGDGITEMYEKTRAEGFGPEVQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKQDFETVFAQ
GVDAILTPATPSAAFGLGEMTDADPVAMYLNDIFTVTVNLAGLPGIAVPGGLDANGLPLGLQLIGRPWEEAELLNTAYAL
EQAVGFVAKPQKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=40.5622489959839, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI195972892, Length=499, Percent_Identity=24.8496993987976, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI166795287, Length=157, Percent_Identity=31.8471337579618, Blast_Score=66, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17543272, Length=447, Percent_Identity=37.1364653243848, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=512, Percent_Identity=25, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=313, Percent_Identity=26.8370607028754, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=245, Percent_Identity=27.3469387755102, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=154, Percent_Identity=33.1168831168831, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=210, Percent_Identity=30, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=429, Percent_Identity=35.8974358974359, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319685, Length=466, Percent_Identity=25.7510729613734, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648113, Length=481, Percent_Identity=38.4615384615385, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24652985, Length=499, Percent_Identity=25.0501002004008, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922090, Length=499, Percent_Identity=25.0501002004008, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652981, Length=499, Percent_Identity=25.0501002004008, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652983, Length=499, Percent_Identity=25.0501002004008, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24644968, Length=500, Percent_Identity=25.2, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI161078093, Length=526, Percent_Identity=25.2851711026616, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550774, Length=258, Percent_Identity=27.5193798449612, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648435, Length=258, Percent_Identity=27.5193798449612, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648437, Length=258, Percent_Identity=27.5193798449612, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356731, Length=510, Percent_Identity=24.3137254901961, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648441, Length=181, Percent_Identity=29.8342541436464, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648439, Length=181, Percent_Identity=29.8342541436464, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_ROSDO (Q168I9)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682290.1
- ProteinModelPortal:   Q168I9
- SMR:   Q168I9
- STRING:   Q168I9
- GeneID:   4196746
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2000
- NMPDR:   fig|375451.6.peg.1868
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q168I9
- ProtClustDB:   PRK00012
- BioCyc:   RDEN375451:RD1_2000-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52424; Mature: 52293

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNELGLAEARDALRAGETTSKDLTEACLTAIEEAGVLNAFVHHTADLALERAAAADA
CCCCHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAQGAAPAMCGLPIGMKDLFCTKGVASQAGSRILEGFLPEYESTVSQNLVDAGAVMLGK
HHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEC
LNMDEFAMGSSNETSIYGDVINPWRRKDDEKPLTPGGSSGGSAAAVAADLCLAATGTDTG
CCHHHHCCCCCCCCEEECCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKSVRDAAIMLEAMSGHDPK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
DSTSADLAVPDFEAALSGDIRGKKIGIPKEYRMDGMPDEIEALWQTGIAMMKDAGAEIID
CCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
ISLPHTKYALPAYYVIAPAEASSNLARYDGVRYGHRAKLDQGDGITEMYEKTRAEGFGPE
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
VQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKQDFETVFAQGVDAILTPATPSAAFGLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCCCC
MTDADPVAMYLNDIFTVTVNLAGLPGIAVPGGLDANGLPLGLQLIGRPWEEAELLNTAYA
CCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
LEQAVGFVAKPQKWW
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKLNELGLAEARDALRAGETTSKDLTEACLTAIEEAGVLNAFVHHTADLALERAAAADA
CCCHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAQGAAPAMCGLPIGMKDLFCTKGVASQAGSRILEGFLPEYESTVSQNLVDAGAVMLGK
HHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEC
LNMDEFAMGSSNETSIYGDVINPWRRKDDEKPLTPGGSSGGSAAAVAADLCLAATGTDTG
CCHHHHCCCCCCCCEEECCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKSVRDAAIMLEAMSGHDPK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
DSTSADLAVPDFEAALSGDIRGKKIGIPKEYRMDGMPDEIEALWQTGIAMMKDAGAEIID
CCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
ISLPHTKYALPAYYVIAPAEASSNLARYDGVRYGHRAKLDQGDGITEMYEKTRAEGFGPE
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
VQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKQDFETVFAQGVDAILTPATPSAAFGLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCCCC
MTDADPVAMYLNDIFTVTVNLAGLPGIAVPGGLDANGLPLGLQLIGRPWEEAELLNTAYA
CCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
LEQAVGFVAKPQKWW
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA