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The map label for this gene is nthB [H]

Identifier: 110679198

GI number: 110679198

Start: 1821831

End: 1822505

Strand: Direct

Name: nthB [H]

Synonym: RD1_1909

Alternate gene names: 110679198

Gene position: 1821831-1822505 (Clockwise)

Preceding gene: 110679197

Following gene: 110679199

Centisome position: 44.08

GC content: 62.52

Gene sequence:

>675_bases
ATGACACGTCCGCACGACATGGGCGGGCGGTATGGCGACGGCCCTGTCATACCGGAAAATGAGAACCTGCGCTTCAAAGA
GGACTGGCATGCGCGTGCCTTGGCCGTGACGCTGGCCTCTGGCAGTCTGGGTCAGTGGAACATCGACATCTCACGGCACG
CGCGTGAAAAACTCTCCCCCACGGATTATGCGCGCTTTAGCTATTACGAAAAATGGATAGCCGGGTTGGCCGATTTGCTG
GTGCACAGTGGTGTTGTCGATCCACGTGAGTACGCGGGCGCGGACCCGGTGCCAAGCCCTCTGGCGGATCGGGCCTTGCG
CGCGCAAGACGTGGACAAAGTGCTTGCAACTGGCGGGCCTGCGGACCGACCGTCGCAGGTTCCACAGCTTTTCAGTGTCG
GCGACGCCGTCAAAACGCGAAAGGTTGCGGCGAACTGCCTTGTCGATGGCGGCCACACGCGCTTGCCGCGCTACGCGGCG
GGGATGCGGGGCCGCATCGTCCGTTTCCACGGTACGCATGTCTTGCCGGACAGTGCGGCGCATCGGCTTGGCGATGCACC
CGAACCGCTTTACGCTGTTGCTTTTCGCGCCGCCGAACTCTGGGCGCATCCTGAGCATCCGGAGGATGAAGTCGTGCTGG
ATCTGTGGCAAAGCTATCTGGTGGCGGACGCATGA

Upstream 100 bases:

>100_bases
ATGGCACCGACGGATTGGATGAGAAGGCCTTGATGGACCTCGTCACCCGCGACAGCATGATCGGCACGGGCCTCGCGCAC
TACCCCGCGGCGGGCACGAC

Downstream 100 bases:

>100_bases
CACCGCCGGAACCTGTGTTTTCAGAACCCTGGCACGCGCAGGTTTTCGCCGTCACCGTGCGGTTGAATGAAACGGGCCAT
TTGCCTTGGCCCGAGTGGGC

Product: nitrile hydratase, beta subunit

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTRPHDMGGRYGDGPVIPENENLRFKEDWHARALAVTLASGSLGQWNIDISRHAREKLSPTDYARFSYYEKWIAGLADLL
VHSGVVDPREYAGADPVPSPLADRALRAQDVDKVLATGGPADRPSQVPQLFSVGDAVKTRKVAANCLVDGGHTRLPRYAA
GMRGRIVRFHGTHVLPDSAAHRLGDAPEPLYAVAFRAAELWAHPEHPEDEVVLDLWQSYLVADA

Sequences:

>Translated_224_residues
MTRPHDMGGRYGDGPVIPENENLRFKEDWHARALAVTLASGSLGQWNIDISRHAREKLSPTDYARFSYYEKWIAGLADLL
VHSGVVDPREYAGADPVPSPLADRALRAQDVDKVLATGGPADRPSQVPQLFSVGDAVKTRKVAANCLVDGGHTRLPRYAA
GMRGRIVRFHGTHVLPDSAAHRLGDAPEPLYAVAFRAAELWAHPEHPEDEVVLDLWQSYLVADA
>Mature_223_residues
TRPHDMGGRYGDGPVIPENENLRFKEDWHARALAVTLASGSLGQWNIDISRHAREKLSPTDYARFSYYEKWIAGLADLLV
HSGVVDPREYAGADPVPSPLADRALRAQDVDKVLATGGPADRPSQVPQLFSVGDAVKTRKVAANCLVDGGHTRLPRYAAG
MRGRIVRFHGTHVLPDSAAHRLGDAPEPLYAVAFRAAELWAHPEHPEDEVVLDLWQSYLVADA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 24601; Mature: 24470

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPHDMGGRYGDGPVIPENENLRFKEDWHARALAVTLASGSLGQWNIDISRHAREKLSP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECHHHHHHHHCCC
TDYARFSYYEKWIAGLADLLVHSGVVDPREYAGADPVPSPLADRALRAQDVDKVLATGGP
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ADRPSQVPQLFSVGDAVKTRKVAANCLVDGGHTRLPRYAAGMRGRIVRFHGTHVLPDSAA
CCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCCCCCEEEEECCEECCCHHH
HRLGDAPEPLYAVAFRAAELWAHPEHPEDEVVLDLWQSYLVADA
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRPHDMGGRYGDGPVIPENENLRFKEDWHARALAVTLASGSLGQWNIDISRHAREKLSP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECHHHHHHHHCCC
TDYARFSYYEKWIAGLADLLVHSGVVDPREYAGADPVPSPLADRALRAQDVDKVLATGGP
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ADRPSQVPQLFSVGDAVKTRKVAANCLVDGGHTRLPRYAAGMRGRIVRFHGTHVLPDSAA
CCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHCCCCCCEEEEECCEECCCHHH
HRLGDAPEPLYAVAFRAAELWAHPEHPEDEVVLDLWQSYLVADA
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]