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The map label for this gene is nthA [H]

Identifier: 110679197

GI number: 110679197

Start: 1821202

End: 1821834

Strand: Direct

Name: nthA [H]

Synonym: RD1_1908

Alternate gene names: 110679197

Gene position: 1821202-1821834 (Clockwise)

Preceding gene: 110679192

Following gene: 110679198

Centisome position: 44.06

GC content: 61.3

Gene sequence:

>633_bases
ATGCCGCATGATCACGATGACCACACTCACCCGCATACTCTGCTGCCCAGCGACCCTGCGCTGCGCATCAAGGCTCTGGA
AACGCTGCTGACCCGAAAAGGGCTGATTGATCCGCAAGCGCTGGATGCGATCATTGATACCTATGAAAACAGGATCGGCC
CGCGCAACGGAGCGCATGTGGTGGTCAAAGCATGGACGGACCCCGCGTTCCGCGCCGCCTTGCTGGAGGATGCTACAAAG
GTCGTGTCCGAACTGGGATATTATGGGCGTCAGGGCGAACATATGGTGGCCGTTGAAAACACGGATAGCGTTCATAACAT
GGTCGTGTGCACCCTGTGCAGCTGTTATCCTTGGCCGCTGCTCGGCATTCCGCCGACTTGGTATAAATCGGATGCGTATC
GCGCCCGCGCGGTACGCGAGCCGCGAAAGGTGCTGGCGGACTTTGGGGTCGCCTTGCCCTCTGACGTGGCCGTGCGCGTC
TGGGACAGCACAGCCGAGGTGCGCTATCTGGTCATTCCGCGACGTCCCGATGGCACCGACGGATTGGATGAGAAGGCCTT
GATGGACCTCGTCACCCGCGACAGCATGATCGGCACGGGCCTCGCGCACTACCCCGCGGCGGGCACGACATGA

Upstream 100 bases:

>100_bases
CTTTGCCGGCATGAAGCCCGGGGCCCTTTGGTGGCCAGGTTCAGCATTTGCCATCCAAAGCGACTGGACAAACATGGCCG
CTTGGACGCATCCTTGCGTC

Downstream 100 bases:

>100_bases
CACGTCCGCACGACATGGGCGGGCGGTATGGCGACGGCCCTGTCATACCGGAAAATGAGAACCTGCGCTTCAAAGAGGAC
TGGCATGCGCGTGCCTTGGC

Product: nitrile hydratase, alpha subunit

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPHDHDDHTHPHTLLPSDPALRIKALETLLTRKGLIDPQALDAIIDTYENRIGPRNGAHVVVKAWTDPAFRAALLEDATK
VVSELGYYGRQGEHMVAVENTDSVHNMVVCTLCSCYPWPLLGIPPTWYKSDAYRARAVREPRKVLADFGVALPSDVAVRV
WDSTAEVRYLVIPRRPDGTDGLDEKALMDLVTRDSMIGTGLAHYPAAGTT

Sequences:

>Translated_210_residues
MPHDHDDHTHPHTLLPSDPALRIKALETLLTRKGLIDPQALDAIIDTYENRIGPRNGAHVVVKAWTDPAFRAALLEDATK
VVSELGYYGRQGEHMVAVENTDSVHNMVVCTLCSCYPWPLLGIPPTWYKSDAYRARAVREPRKVLADFGVALPSDVAVRV
WDSTAEVRYLVIPRRPDGTDGLDEKALMDLVTRDSMIGTGLAHYPAAGTT
>Mature_209_residues
PHDHDDHTHPHTLLPSDPALRIKALETLLTRKGLIDPQALDAIIDTYENRIGPRNGAHVVVKAWTDPAFRAALLEDATKV
VSELGYYGRQGEHMVAVENTDSVHNMVVCTLCSCYPWPLLGIPPTWYKSDAYRARAVREPRKVLADFGVALPSDVAVRVW
DSTAEVRYLVIPRRPDGTDGLDEKALMDLVTRDSMIGTGLAHYPAAGTT

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 23203; Mature: 23071

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHDHDDHTHPHTLLPSDPALRIKALETLLTRKGLIDPQALDAIIDTYENRIGPRNGAHV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEE
VVKAWTDPAFRAALLEDATKVVSELGYYGRQGEHMVAVENTDSVHNMVVCTLCSCYPWPL
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
LGIPPTWYKSDAYRARAVREPRKVLADFGVALPSDVAVRVWDSTAEVRYLVIPRRPDGTD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCC
GLDEKALMDLVTRDSMIGTGLAHYPAAGTT
CCHHHHHHHHHHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
PHDHDDHTHPHTLLPSDPALRIKALETLLTRKGLIDPQALDAIIDTYENRIGPRNGAHV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEE
VVKAWTDPAFRAALLEDATKVVSELGYYGRQGEHMVAVENTDSVHNMVVCTLCSCYPWPL
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
LGIPPTWYKSDAYRARAVREPRKVLADFGVALPSDVAVRVWDSTAEVRYLVIPRRPDGTD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCC
GLDEKALMDLVTRDSMIGTGLAHYPAAGTT
CCHHHHHHHHHHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]