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The map label for this gene is wcaJ [H]

Identifier: 110679002

GI number: 110679002

Start: 1632471

End: 1633922

Strand: Reverse

Name: wcaJ [H]

Synonym: RD1_1699

Alternate gene names: 110679002

Gene position: 1633922-1632471 (Counterclockwise)

Preceding gene: 110679003

Following gene: 110678994

Centisome position: 39.53

GC content: 57.37

Gene sequence:

>1452_bases
ATGAGTAAAATGGACAGGCACTCAATCGAGTTGAATGAAGCCGCCCGAAATGTTGCCCTTGTGCTGAAACGCGGCGCGCT
CGAACCACTTTGGCTTGCCTGTATCGCCGTTTGTATCGACGTGTTCGTTTTCGCCCTGGCCTTCTGGTTTGCGCTCTACG
GGGCCACGCCAACGGAACAATTCGCGCCCGTCGGTGCGGCGGGCTGGGCCATTCTGGGCAGTGGCGTGGTTGTTGCATCC
ATGGTTGCCATTGGCGGATATCGCAGTGCGGTCATGGCCAATCGGGTGTCCTTCCTCACCTATAGCCTGCTGTGCGCTGC
TGGGCCAATTGCTGCAGGCATTGCCTTTTCGCCCGTCACAAACCTGAGCACGGCATTCGCAAGCGCCGCTTTGAGTTTGA
CAGTTGCGATCATCCCGACCCGGCTTGGCGGCAATATGATCATCAGATGGGTCATCGAAACCGGTTTGATCGCACGTCGC
GCCGTCATCGCCGGTGGCGGCGAGGAAGCGGCACGCCTTATCCGTGGGCTGAATGCGCGACCGGGCAACGATATTCGCCT
GCACGGCATATTTGATGACCGTGATGATATACGCTCGCCCAAACAGGTTCTGGGCGTTCCCAAGATCGGGGGGTACAAGG
ACCTGATCGCCTTTGTGCGCGCTGCAGACATTGAACTGGTGATCATTGCCCTACCCTTTGCCGCAGAAAAACGCATCAAG
TGGCTGTTGGATGAATTCCGTGTCCTTCCGGTCGAAGTTGGGCTGTCGACCTACAGCAAGGATTTTGCCTTTGATCAAAA
CCGGCGGGCGCCATTGCTGGCGAGGGTCAAGCGCAGCTATGCGCCCGAGTGTCGTTTGGCCAAACGTCTCTTTGATGTGT
TCTTTGCGCTCTGTGCCCTTGCGGTTTTTTGGCCTGTGATGGCCTTGGCGGCCCTCGCCATTCGTCTGGAATCTCCTGGC
CCCGTCCTGTTTCGACAGCACCGCCACGGCTATAACAACCGCATTATCGAAGTGCTGAAATTTCGCTCGATGTACACGGA
AATGAGCGATCCGTTGGGCAAGAATGTGGTGACCAAGGACGATCCCCGTGTAACGCGGGTCGGGCGCTTCCTGCGGCGCT
CTTCGATTGACGAACTTCCCCAGCTTTTCAATGTGTTGCGCGGCGACTTGTCACTCGTCGGGCCGCGCCCCCACGCCGTC
GAAGCAATGTCCAGTCAACATGAGCGCTTCACCCAGATCGTGGATGGGTATTCGGCCCGCCACCGGCTGCCACCCGGCAT
TACCGGCTGGGCTCAAATCAACGGTTGGCGTGGCGAGATTGATGACGCCAAAAAGCTGGAAGCCCGCTTTGAACACGATC
TGTTTTACATTGAAAACTGGTCAATCTGGCTGGATCTCAAGATCCTGCTCCAAACCCCTTTAAGCCTGTTCCGGGCAAAA
GGCGCGTATTGA

Upstream 100 bases:

>100_bases
CCGTTTCGACCAACTCAAACGGAGTCTTAACGGATAGGGTGCTAGGCTGCACGCTGGAAGTGCCGGATAGGACCCGGTTC
AGCGTAGGATAACCGCACCG

Downstream 100 bases:

>100_bases
AGCGTCTGGCGAAATACCTGAAACATCATGCATCACGTAACGTGTTGAAATCCTTGAATTCAGGCAGCTTTACGTGATCG
AGGCTTTTATCATGACGATT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSKMDRHSIELNEAARNVALVLKRGALEPLWLACIAVCIDVFVFALAFWFALYGATPTEQFAPVGAAGWAILGSGVVVAS
MVAIGGYRSAVMANRVSFLTYSLLCAAGPIAAGIAFSPVTNLSTAFASAALSLTVAIIPTRLGGNMIIRWVIETGLIARR
AVIAGGGEEAARLIRGLNARPGNDIRLHGIFDDRDDIRSPKQVLGVPKIGGYKDLIAFVRAADIELVIIALPFAAEKRIK
WLLDEFRVLPVEVGLSTYSKDFAFDQNRRAPLLARVKRSYAPECRLAKRLFDVFFALCALAVFWPVMALAALAIRLESPG
PVLFRQHRHGYNNRIIEVLKFRSMYTEMSDPLGKNVVTKDDPRVTRVGRFLRRSSIDELPQLFNVLRGDLSLVGPRPHAV
EAMSSQHERFTQIVDGYSARHRLPPGITGWAQINGWRGEIDDAKKLEARFEHDLFYIENWSIWLDLKILLQTPLSLFRAK
GAY

Sequences:

>Translated_483_residues
MSKMDRHSIELNEAARNVALVLKRGALEPLWLACIAVCIDVFVFALAFWFALYGATPTEQFAPVGAAGWAILGSGVVVAS
MVAIGGYRSAVMANRVSFLTYSLLCAAGPIAAGIAFSPVTNLSTAFASAALSLTVAIIPTRLGGNMIIRWVIETGLIARR
AVIAGGGEEAARLIRGLNARPGNDIRLHGIFDDRDDIRSPKQVLGVPKIGGYKDLIAFVRAADIELVIIALPFAAEKRIK
WLLDEFRVLPVEVGLSTYSKDFAFDQNRRAPLLARVKRSYAPECRLAKRLFDVFFALCALAVFWPVMALAALAIRLESPG
PVLFRQHRHGYNNRIIEVLKFRSMYTEMSDPLGKNVVTKDDPRVTRVGRFLRRSSIDELPQLFNVLRGDLSLVGPRPHAV
EAMSSQHERFTQIVDGYSARHRLPPGITGWAQINGWRGEIDDAKKLEARFEHDLFYIENWSIWLDLKILLQTPLSLFRAK
GAY
>Mature_482_residues
SKMDRHSIELNEAARNVALVLKRGALEPLWLACIAVCIDVFVFALAFWFALYGATPTEQFAPVGAAGWAILGSGVVVASM
VAIGGYRSAVMANRVSFLTYSLLCAAGPIAAGIAFSPVTNLSTAFASAALSLTVAIIPTRLGGNMIIRWVIETGLIARRA
VIAGGGEEAARLIRGLNARPGNDIRLHGIFDDRDDIRSPKQVLGVPKIGGYKDLIAFVRAADIELVIIALPFAAEKRIKW
LLDEFRVLPVEVGLSTYSKDFAFDQNRRAPLLARVKRSYAPECRLAKRLFDVFFALCALAVFWPVMALAALAIRLESPGP
VLFRQHRHGYNNRIIEVLKFRSMYTEMSDPLGKNVVTKDDPRVTRVGRFLRRSSIDELPQLFNVLRGDLSLVGPRPHAVE
AMSSQHERFTQIVDGYSARHRLPPGITGWAQINGWRGEIDDAKKLEARFEHDLFYIENWSIWLDLKILLQTPLSLFRAKG
AY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=392, Percent_Identity=36.4795918367347, Blast_Score=225, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53498; Mature: 53367

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMDRHSIELNEAARNVALVLKRGALEPLWLACIAVCIDVFVFALAFWFALYGATPTEQ
CCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FAPVGAAGWAILGSGVVVASMVAIGGYRSAVMANRVSFLTYSLLCAAGPIAAGIAFSPVT
CCCCCCCHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHH
NLSTAFASAALSLTVAIIPTRLGGNMIIRWVIETGLIARRAVIAGGGEEAARLIRGLNAR
HHHHHHHHHHHHEEEEEEEECCCCCCEEHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCC
PGNDIRLHGIFDDRDDIRSPKQVLGVPKIGGYKDLIAFVRAADIELVIIALPFAAEKRIK
CCCCEEEEEECCCHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
WLLDEFRVLPVEVGLSTYSKDFAFDQNRRAPLLARVKRSYAPECRLAKRLFDVFFALCAL
HHHHHHHEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AVFWPVMALAALAIRLESPGPVLFRQHRHGYNNRIIEVLKFRSMYTEMSDPLGKNVVTKD
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DPRVTRVGRFLRRSSIDELPQLFNVLRGDLSLVGPRPHAVEAMSSQHERFTQIVDGYSAR
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHCCCHHC
HRLPPGITGWAQINGWRGEIDDAKKLEARFEHDLFYIENWSIWLDLKILLQTPLSLFRAK
CCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEHHHHHHCHHHHHHCC
GAY
CCC
>Mature Secondary Structure 
SKMDRHSIELNEAARNVALVLKRGALEPLWLACIAVCIDVFVFALAFWFALYGATPTEQ
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FAPVGAAGWAILGSGVVVASMVAIGGYRSAVMANRVSFLTYSLLCAAGPIAAGIAFSPVT
CCCCCCCHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHH
NLSTAFASAALSLTVAIIPTRLGGNMIIRWVIETGLIARRAVIAGGGEEAARLIRGLNAR
HHHHHHHHHHHHEEEEEEEECCCCCCEEHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCC
PGNDIRLHGIFDDRDDIRSPKQVLGVPKIGGYKDLIAFVRAADIELVIIALPFAAEKRIK
CCCCEEEEEECCCHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
WLLDEFRVLPVEVGLSTYSKDFAFDQNRRAPLLARVKRSYAPECRLAKRLFDVFFALCAL
HHHHHHHEEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AVFWPVMALAALAIRLESPGPVLFRQHRHGYNNRIIEVLKFRSMYTEMSDPLGKNVVTKD
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DPRVTRVGRFLRRSSIDELPQLFNVLRGDLSLVGPRPHAVEAMSSQHERFTQIVDGYSAR
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHCCCHHC
HRLPPGITGWAQINGWRGEIDDAKKLEARFEHDLFYIENWSIWLDLKILLQTPLSLFRAK
CCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEHHHHHHCHHHHHHCC
GAY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]