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The map label for this gene is yafV [C]

Identifier: 110678808

GI number: 110678808

Start: 1421946

End: 1422731

Strand: Reverse

Name: yafV [C]

Synonym: RD1_1496

Alternate gene names: 110678808

Gene position: 1422731-1421946 (Counterclockwise)

Preceding gene: 110678809

Following gene: 110678807

Centisome position: 34.42

GC content: 60.94

Gene sequence:

>786_bases
ATGAAACTCGCGCTGTGGCAGACCCATCCGAGAACAGGCATCGCCGCGGCACTGACGGCGCTGCGCGAGGCTGCGACATC
CGCCGCCACGCAAGGGGCGGATGTGTTGATCACGCCTGAAATGTTCGTGGGCGGGTATAACATCGGGCCGGAGCGCATCG
CCACACATGCAGATCATGCCGCAGAGGTGCTGGACAGCCTCACGTCGATTGCCAAGACGCAGGACATCGCATTGGTCGTG
GGCCTGACCCTGCCCGCGCCCCCCCTGCCCCATAACGCCTGCGTGGTCATCGACAACACGGGCACGCAGGTCGCCCGCTA
TCACAAAACGCATCTTTTCGGGGATGTGGATCGCGCGCAGTTCAGCGCGGGTGCCGCACTGTCAGAGGTCTTTGACTTAG
CGGGTTGGAAGGTCGGCCTCGCGATTTGCTATGACGTTGAATTCCCCGAACTGATAAGATCGCTGGCGCTGCGCGGAGCC
GAGGTTATCCTGACACCCACCGCCAATATGGAACCCTTTGACAGCATCAACACACGTCTGGTCCCGGCCCGCGCAGAGGA
AAACGGCGTTTACGTGGCCTATTGCAACTACATCGGGGCCGAAGCGCAATTCACCTATAATGGCCTGTCCTGTCTATCCG
GGCCGGACGGCCAGGATCATGTGAGAGCAGAAAAGGCAGAAGCGATGCTCTTTGCGACCCTCGACCGGGCGGACCTCGCG
CGTGCACGGCAATCGCAAACCCATCTGCAGGATCGTCGCCCTGACCTTTATGGAGACATCACATGA

Upstream 100 bases:

>100_bases
AGTTTACCTCGCGCACAAACGCGCAGAGATCGCACATCTGGCGGACATGGACACCGATGCGAAATGTGCCGCCTATGCCG
ACACGTACTGAGGATACCCG

Downstream 100 bases:

>100_bases
AGCCTGTCACCGTCTTTGGACCCGACTTTCCCTTTGCCTATGACGACTGGATCGCGCATCCCGACGGGCTGGCCACTTTG
CCTGCCGCAGCGCACGGCGC

Product: hydrolase, putative

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKLALWQTHPRTGIAAALTALREAATSAATQGADVLITPEMFVGGYNIGPERIATHADHAAEVLDSLTSIAKTQDIALVV
GLTLPAPPLPHNACVVIDNTGTQVARYHKTHLFGDVDRAQFSAGAALSEVFDLAGWKVGLAICYDVEFPELIRSLALRGA
EVILTPTANMEPFDSINTRLVPARAEENGVYVAYCNYIGAEAQFTYNGLSCLSGPDGQDHVRAEKAEAMLFATLDRADLA
RARQSQTHLQDRRPDLYGDIT

Sequences:

>Translated_261_residues
MKLALWQTHPRTGIAAALTALREAATSAATQGADVLITPEMFVGGYNIGPERIATHADHAAEVLDSLTSIAKTQDIALVV
GLTLPAPPLPHNACVVIDNTGTQVARYHKTHLFGDVDRAQFSAGAALSEVFDLAGWKVGLAICYDVEFPELIRSLALRGA
EVILTPTANMEPFDSINTRLVPARAEENGVYVAYCNYIGAEAQFTYNGLSCLSGPDGQDHVRAEKAEAMLFATLDRADLA
RARQSQTHLQDRRPDLYGDIT
>Mature_261_residues
MKLALWQTHPRTGIAAALTALREAATSAATQGADVLITPEMFVGGYNIGPERIATHADHAAEVLDSLTSIAKTQDIALVV
GLTLPAPPLPHNACVVIDNTGTQVARYHKTHLFGDVDRAQFSAGAALSEVFDLAGWKVGLAICYDVEFPELIRSLALRGA
EVILTPTANMEPFDSINTRLVPARAEENGVYVAYCNYIGAEAQFTYNGLSCLSGPDGQDHVRAEKAEAMLFATLDRADLA
RARQSQTHLQDRRPDLYGDIT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=258, Percent_Identity=32.5581395348837, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI5031947, Length=189, Percent_Identity=34.9206349206349, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI297632348, Length=189, Percent_Identity=34.9206349206349, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI297632350, Length=189, Percent_Identity=34.9206349206349, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1786412, Length=224, Percent_Identity=29.0178571428571, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=276, Percent_Identity=28.6231884057971, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21355835, Length=271, Percent_Identity=26.1992619926199, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 28087; Mature: 28087

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLALWQTHPRTGIAAALTALREAATSAATQGADVLITPEMFVGGYNIGPERIATHADHA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHEECCCCCCHHHHHHHHHHH
AEVLDSLTSIAKTQDIALVVGLTLPAPPLPHNACVVIDNTGTQVARYHKTHLFGDVDRAQ
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHH
FSAGAALSEVFDLAGWKVGLAICYDVEFPELIRSLALRGAEVILTPTANMEPFDSINTRL
HHCCHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEE
VPARAEENGVYVAYCNYIGAEAQFTYNGLSCLSGPDGQDHVRAEKAEAMLFATLDRADLA
EECCCCCCCEEEEEECCCCCCCEEEECCEEECCCCCCHHHHHHHHHHEEEEEECCHHHHH
RARQSQTHLQDRRPDLYGDIT
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLALWQTHPRTGIAAALTALREAATSAATQGADVLITPEMFVGGYNIGPERIATHADHA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHEECCCCCCHHHHHHHHHHH
AEVLDSLTSIAKTQDIALVVGLTLPAPPLPHNACVVIDNTGTQVARYHKTHLFGDVDRAQ
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHH
FSAGAALSEVFDLAGWKVGLAICYDVEFPELIRSLALRGAEVILTPTANMEPFDSINTRL
HHCCHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEE
VPARAEENGVYVAYCNYIGAEAQFTYNGLSCLSGPDGQDHVRAEKAEAMLFATLDRADLA
EECCCCCCCEEEEEECCCCCCCEEEECCEEECCCCCCHHHHHHHHHHEEEEEECCHHHHH
RARQSQTHLQDRRPDLYGDIT
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8526497 [H]