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The map label for this gene is merA [H]

Identifier: 110678778

GI number: 110678778

Start: 1387814

End: 1389292

Strand: Direct

Name: merA [H]

Synonym: RD1_1464

Alternate gene names: 110678778

Gene position: 1387814-1389292 (Clockwise)

Preceding gene: 110678777

Following gene: 110678779

Centisome position: 33.58

GC content: 60.38

Gene sequence:

>1479_bases
GTGCGCTGTCAGTGCTTCCCGTCCTCATCAAGGCCATCACTGGCAAGAAAGACCTCTGACATGAAACGTATCAAAACCGA
TGTCCTGATCATTGGCGCCGGGTCGGGTGGTTTGTCCGTCGCCGCGGGTGCCGCCCAGATGGGGGCAAATGTTGTCCTGC
TTGAAGGGCATAAGATGGGCGGCGATTGTCTGAATTTCGGCTGCGTTCCATCGAAGGCGCTGATTGCCAGCGGTAAGGCG
GCGCAAAGCCAGCGCAATGCGTCCCAATACGGCGTCGCCAATGGGCCGGGTGCGGCGGATTATGCCGCCACCAAGGACCA
CGTGCATGACGTCATCGCGCAAATTGCGCCGGTCGACAGTCAGGAGCGGTTCGAAGGCTTTGGCATCAACGTCATCCGTG
AGTACGGGCGTTTCATTTCGCCCACTGAGGTGCAGGCGGGCGAGACGGTGATCGCCGCCCGTCGCGTGGTGATCGCCACC
GGCTCATCACCGCTGGTGCCTCCGATCCCGGGGCTGGGCGATGTGCCTTTCGAGACGAACGAAACGATTTTCGATCTGCG
CGACAAGCCGGAACATCTGCTGATCATTGGCGGTGGCCCGATTGGCATGGAAATGGCGCAGGCGCACATCCGCCTTGGCT
GCGAGGTAACGGTGATCGAGGGCGCGCAGGCCCTCGGCAAGGATGATCCCGAACTGGCGGCGGTCGTTCTGGACAGTCTG
CGCGCAGAAGGCGTCGTGATCAGGGAACAGACCAAGGCCGCACAGATCAAAGGGGCGGCGGGTGCGATCGACGTCATCAC
GCAGGACGGTGAAACGATTTCAGGCACGCATCTTTTGATGGCCGTGGGGCGCAAGGCGAATATTGACACCCTCGGGCTTG
AGGCAGGCGGGATCAAAACCAAGGGAAACGGAATTGAGGTAGATGCAGGTCTGCGCACCAGCAACCGCAGGGTCTATGCC
ATTGGCGATGTGGCAGGCGGGCTGCAGTTTACGCATGTGGCGGGGTATCATGCGGGTGTGATCATCCGATCCATGCTCTT
TGGACTGCCGTCAAAGGCCAAGGTGTCCCACATCCCATGGGCGACCTATACGGAGCCTGAACTGGCGCAGGTCGGATTGA
CCGAAGCACAGGCGCGTAAGGAACATGGGACCGCTCTGGAGGTCGTGCGCTTTGACTATGCCCATAATGATCGCGCCATT
GCAGAGCGCAAGACCACAGGGTTCATCAAGCTGATGGTGCACAAGGGCAGACCGGTGGGCGCGTCCATCGTCGGGTATCA
GGCGGGCGAGTTGATCAACCTCTGGGCGCTGGCGCTGGCCAACCGTATGAAGATGAGCCAGATCGCCGCCATGGTCGCCC
CCTATCCCACCATCGGAGAGATCAACAAACGCGCCGCAGGGGCCTATTACAGTCCGCGACTTTTCGACAATCCCAAGGTC
AAAAAGATCGTGGGGCTTGTTCAACGCTACATCCCGTGA

Upstream 100 bases:

>100_bases
TCGGTGGGTGCGGGGCTGGGGGAGGTGTTCGCCCGAGGCGAGACGCCCAACTTCGGCATCATCTTTGAACCACATATATT
ACTGCCGATCCTGGGGTTGA

Downstream 100 bases:

>100_bases
AAATCTGACATATGTTCAATTCGCTTTCAGGTCGTCTCATCATTCTGACAACCATCTTTGTGATGATTGCAGAGGTCCTG
ATTTTTGTGCCCTCGATCGC

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MRCQCFPSSSRPSLARKTSDMKRIKTDVLIIGAGSGGLSVAAGAAQMGANVVLLEGHKMGGDCLNFGCVPSKALIASGKA
AQSQRNASQYGVANGPGAADYAATKDHVHDVIAQIAPVDSQERFEGFGINVIREYGRFISPTEVQAGETVIAARRVVIAT
GSSPLVPPIPGLGDVPFETNETIFDLRDKPEHLLIIGGGPIGMEMAQAHIRLGCEVTVIEGAQALGKDDPELAAVVLDSL
RAEGVVIREQTKAAQIKGAAGAIDVITQDGETISGTHLLMAVGRKANIDTLGLEAGGIKTKGNGIEVDAGLRTSNRRVYA
IGDVAGGLQFTHVAGYHAGVIIRSMLFGLPSKAKVSHIPWATYTEPELAQVGLTEAQARKEHGTALEVVRFDYAHNDRAI
AERKTTGFIKLMVHKGRPVGASIVGYQAGELINLWALALANRMKMSQIAAMVAPYPTIGEINKRAAGAYYSPRLFDNPKV
KKIVGLVQRYIP

Sequences:

>Translated_492_residues
MRCQCFPSSSRPSLARKTSDMKRIKTDVLIIGAGSGGLSVAAGAAQMGANVVLLEGHKMGGDCLNFGCVPSKALIASGKA
AQSQRNASQYGVANGPGAADYAATKDHVHDVIAQIAPVDSQERFEGFGINVIREYGRFISPTEVQAGETVIAARRVVIAT
GSSPLVPPIPGLGDVPFETNETIFDLRDKPEHLLIIGGGPIGMEMAQAHIRLGCEVTVIEGAQALGKDDPELAAVVLDSL
RAEGVVIREQTKAAQIKGAAGAIDVITQDGETISGTHLLMAVGRKANIDTLGLEAGGIKTKGNGIEVDAGLRTSNRRVYA
IGDVAGGLQFTHVAGYHAGVIIRSMLFGLPSKAKVSHIPWATYTEPELAQVGLTEAQARKEHGTALEVVRFDYAHNDRAI
AERKTTGFIKLMVHKGRPVGASIVGYQAGELINLWALALANRMKMSQIAAMVAPYPTIGEINKRAAGAYYSPRLFDNPKV
KKIVGLVQRYIP
>Mature_492_residues
MRCQCFPSSSRPSLARKTSDMKRIKTDVLIIGAGSGGLSVAAGAAQMGANVVLLEGHKMGGDCLNFGCVPSKALIASGKA
AQSQRNASQYGVANGPGAADYAATKDHVHDVIAQIAPVDSQERFEGFGINVIREYGRFISPTEVQAGETVIAARRVVIAT
GSSPLVPPIPGLGDVPFETNETIFDLRDKPEHLLIIGGGPIGMEMAQAHIRLGCEVTVIEGAQALGKDDPELAAVVLDSL
RAEGVVIREQTKAAQIKGAAGAIDVITQDGETISGTHLLMAVGRKANIDTLGLEAGGIKTKGNGIEVDAGLRTSNRRVYA
IGDVAGGLQFTHVAGYHAGVIIRSMLFGLPSKAKVSHIPWATYTEPELAQVGLTEAQARKEHGTALEVVRFDYAHNDRAI
AERKTTGFIKLMVHKGRPVGASIVGYQAGELINLWALALANRMKMSQIAAMVAPYPTIGEINKRAAGAYYSPRLFDNPKV
KKIVGLVQRYIP

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=31.2236286919831, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=28.1995661605206, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI33519430, Length=459, Percent_Identity=26.3616557734205, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI33519428, Length=459, Percent_Identity=26.3616557734205, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI33519426, Length=459, Percent_Identity=26.3616557734205, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI148277071, Length=459, Percent_Identity=26.3616557734205, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI148277065, Length=459, Percent_Identity=26.3616557734205, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI291045266, Length=464, Percent_Identity=25.2155172413793, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI22035672, Length=465, Percent_Identity=24.5161290322581, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI291045268, Length=456, Percent_Identity=24.1228070175439, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=31.5789473684211, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=29.2682926829268, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=28.9351851851852, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082354, Length=457, Percent_Identity=26.4770240700219, Blast_Score=133, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=488, Percent_Identity=29.5081967213115, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=27.8131634819533, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71982272, Length=477, Percent_Identity=26.8343815513627, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=471, Percent_Identity=27.6008492569002, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=428, Percent_Identity=26.6355140186916, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=30.3157894736842, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325166, Length=480, Percent_Identity=26.875, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325240, Length=466, Percent_Identity=24.4635193133047, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358499, Length=482, Percent_Identity=30.2904564315353, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=27.1762208067941, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=27.1762208067941, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=27.1762208067941, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=25.6619144602851, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 51997; Mature: 51997

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCQCFPSSSRPSLARKTSDMKRIKTDVLIIGAGSGGLSVAAGAAQMGANVVLLEGHKMG
CCCEECCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCEEECCHHHHCCCEEEEECCCCC
GDCLNFGCVPSKALIASGKAAQSQRNASQYGVANGPGAADYAATKDHVHDVIAQIAPVDS
CCCEECCCCCCHHHHCCCCHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QERFEGFGINVIREYGRFISPTEVQAGETVIAARRVVIATGSSPLVPPIPGLGDVPFETN
HHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
ETIFDLRDKPEHLLIIGGGPIGMEMAQAHIRLGCEVTVIEGAQALGKDDPELAAVVLDSL
CEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHH
RAEGVVIREQTKAAQIKGAAGAIDVITQDGETISGTHLLMAVGRKANIDTLGLEAGGIKT
CCCCEEEEECCCHHHCCCCCCEEEEEECCCCEECCCEEEEEECCCCCCCEEEECCCCEEE
KGNGIEVDAGLRTSNRRVYAIGDVAGGLQFTHVAGYHAGVIIRSMLFGLPSKAKVSHIPW
CCCCEEEECCCCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCC
ATYTEPELAQVGLTEAQARKEHGTALEVVRFDYAHNDRAIAERKTTGFIKLMVHKGRPVG
CCCCCCHHHHCCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCEEEEEEECCCCCC
ASIVGYQAGELINLWALALANRMKMSQIAAMVAPYPTIGEINKRAAGAYYSPRLFDNPKV
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCCCCCCCCCCCCHH
KKIVGLVQRYIP
HHHHHHHHHHCC
>Mature Secondary Structure
MRCQCFPSSSRPSLARKTSDMKRIKTDVLIIGAGSGGLSVAAGAAQMGANVVLLEGHKMG
CCCEECCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCEEECCHHHHCCCEEEEECCCCC
GDCLNFGCVPSKALIASGKAAQSQRNASQYGVANGPGAADYAATKDHVHDVIAQIAPVDS
CCCEECCCCCCHHHHCCCCHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QERFEGFGINVIREYGRFISPTEVQAGETVIAARRVVIATGSSPLVPPIPGLGDVPFETN
HHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
ETIFDLRDKPEHLLIIGGGPIGMEMAQAHIRLGCEVTVIEGAQALGKDDPELAAVVLDSL
CEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCHHHHHHHHHHH
RAEGVVIREQTKAAQIKGAAGAIDVITQDGETISGTHLLMAVGRKANIDTLGLEAGGIKT
CCCCEEEEECCCHHHCCCCCCEEEEEECCCCEECCCEEEEEECCCCCCCEEEECCCCEEE
KGNGIEVDAGLRTSNRRVYAIGDVAGGLQFTHVAGYHAGVIIRSMLFGLPSKAKVSHIPW
CCCCEEEECCCCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCC
ATYTEPELAQVGLTEAQARKEHGTALEVVRFDYAHNDRAIAERKTTGFIKLMVHKGRPVG
CCCCCCHHHHCCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCEEEEEEECCCCCC
ASIVGYQAGELINLWALALANRMKMSQIAAMVAPYPTIGEINKRAAGAYYSPRLFDNPKV
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCCCCCCCCCCCCHH
KKIVGLVQRYIP
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]