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The map label for this gene is spoT [H]

Identifier: 110678680

GI number: 110678680

Start: 1307760

End: 1309916

Strand: Direct

Name: spoT [H]

Synonym: RD1_1359

Alternate gene names: 110678680

Gene position: 1307760-1309916 (Clockwise)

Preceding gene: 110678679

Following gene: 110678681

Centisome position: 31.64

GC content: 58.69

Gene sequence:

>2157_bases
ATGAAAGACGGCGATCATTTGGAAATATCCGCCGATGACCTCGTGTCGCTCGTTCTGCGCTACAATCCGAAAACCAACCA
GAAGTTGATCCGCGCGGCCTATGACTATGGCCGCGAGATGCATGCGGATCAACTGCGCCATTCGGGTGAGCTGTATTTCA
ATCACCCGGTGGCCGTAGCGGCAATCCTGACCGAACAGAACCTGGATGATGCAACGCTGATCACGGCGCTGCTGCATGAC
ACGATCGAGGACACCCCAGCCTCCTATTCCGAGGTCGCCGCGCGCTTTGGCGACGAGATCGCAGAGCTTGTCGATGGTGT
GACCAAGCTGACCAATCTGCAACTCAACTCGTCACAGACCAAACAGGCCGAGAATTTTCGCAAGCTGTTCATGGCCATGT
CGAAAGACCTGCGGGTCATTCTGGTCAAGCTGTCGGACCGGTTGCACAACATGCGCACCATCCGCGCGATGAAGCCAGAA
AAGCAGGCGCAGAAGGCACGCGAAACAATGGATATCTATGCGCCGTTGGCGGGGCGTATGGGGATGCAGTGGATGCGCGA
AGAACTCGAAGATCTCGCGTTCCGGGTTCTCAATCCGGAGGGGCGCGCGTCCATTATTCGCCGCTTCATCAACCTGCAGA
AGGAAACGGGCGACGTCATTCAGCGCATCACAGGTGACATGCGGCTGGAACTGGACAAGGCCGGAGTGCAGGCCGAAGTC
TTCGGTCGCGCCAAGAAGCCTTATTCGATCTGGCGCAAGATGCAGGAAAAGGACCAGTCCTTTGCACGGCTTTCGGATAT
CTACGGCTTTCGCATCATCACGGACAGCGAAGAAGACTGTTACCGGGCACTGGGCGCCATCCATCAGCGGTGGCGGGCGG
TGCCGGGGCGGTTCAAGGACTACATCAGCCAGCCGAAAAGTAACGGATACCGGTCGATCCATACAACCGTTTCCGGGCGT
GACGGCAAGCGGGTCGAGGTCCAGATCAGAACCGTCCAGATGCATGATGTGGCCGAAACCGGCGTTGCGGCGCATTGGTC
CTATCGGGACGGTGTGCGCAGCAACAATCCCTTTGCTGTTGATCCTGCAAAATGGATCAGCACCCTGACCGAACAGTTCG
ACGCCGAGGAAGATCACGAGGACTTCCTCGAAGCGGTCAAGCTTGAGATGTATTCGGATCAGGTTTTCTGTTTCACGCCC
AAGGGGGAGGTCGTAAAGCTGCCGCGCGGGGCCACGCCGATCGACTTCGCCTTTGCGATCCATACGCGCATCGGCATGGC
CTGTGTGGGGGCCAAGGTTGACCACATGCGCGTACCGCTCTGGACGCGGCTGAAAAACGGCCAGTCGGTGGAGGTGATCA
CGGCGGAGGGTCAAAGCCCGCAGGCCACATGGCTTGACATCGCCGTCACCGGCAAGGCGCGATCTGCTATCCGGCGTGCG
CTGCGCGAGGAAGACCGGGCCCGCTTTGTCAAACTGGGGCAGGAACTGGCGCGCTCCGCGTTCGAGCATGTGGGGCGTAA
GGCGACCGACAAGGCGCTTGAGGCGGCTGCGCGCAACATGCGGTGCAATGATCGCATTGATCTGCTTGCCCGCCTGGGCA
GCGCCGAGATCACCGGCCGCGATGTCGTGCAGGCAGTCTATCCCGAACTTGTGCCGAGGGAAGGCGTGAAAGTGGATGCG
AAGCGCGCGGTGATCGGTCTGTCGCCCGGGCAGTCGTTCGAACGCGCCTTTTGCTGTCAGCCCCTGCCGGGCGAGCGGAT
TGTCGGGATCACCTTCAAGGGGCGCGGAGTGGTGGTGCATGCGATCGATTGCGACCGCATGAGCGTTTACGAAGATCAAC
CGGACCGGTGGCTCGATTTGCGCTGGCACGAAGGCGCGCACCCTGCGGTTTACGGCGCGACGATTGAGCTTACCCTGAAC
AACGATGCAGGGACACTGGGACGAATTTGCACATTGATCGGGGAAACCCGAGCGAATATTTCAGATTTACAATTTGTCGA
CAGAAAGCCTGATTATTTCCTGTTGTTGATCTATGTGGAACTGCGGGATGTGGCGCATCTGCATTCCTTGATGCTGACGC
TTGAAGCGGAAAGTGATGTGGCCTCTGTATCGCGGTATAGAAATATGGCGCATGATAAGACGGAAACACCTGCCTGA

Upstream 100 bases:

>100_bases
GAAGACAGCATGTCCGAAGAGATGTTGCTGCGCCAGTTGATGGCGGCCCAAGGGCAGGGCTGAACGCAGCCCGCCTGATC
TGGTGTGAGAGGCGGTTGAC

Downstream 100 bases:

>100_bases
TGTCGCAGCGCAGTCGTCAGATCATCATAATTTTAACAAGAATGGCGTAAGCCACCCTGTATTGGAATTGAGTAAGGACG
CCCCTCTTGGTTTTTAAACG

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, putative

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MKDGDHLEISADDLVSLVLRYNPKTNQKLIRAAYDYGREMHADQLRHSGELYFNHPVAVAAILTEQNLDDATLITALLHD
TIEDTPASYSEVAARFGDEIAELVDGVTKLTNLQLNSSQTKQAENFRKLFMAMSKDLRVILVKLSDRLHNMRTIRAMKPE
KQAQKARETMDIYAPLAGRMGMQWMREELEDLAFRVLNPEGRASIIRRFINLQKETGDVIQRITGDMRLELDKAGVQAEV
FGRAKKPYSIWRKMQEKDQSFARLSDIYGFRIITDSEEDCYRALGAIHQRWRAVPGRFKDYISQPKSNGYRSIHTTVSGR
DGKRVEVQIRTVQMHDVAETGVAAHWSYRDGVRSNNPFAVDPAKWISTLTEQFDAEEDHEDFLEAVKLEMYSDQVFCFTP
KGEVVKLPRGATPIDFAFAIHTRIGMACVGAKVDHMRVPLWTRLKNGQSVEVITAEGQSPQATWLDIAVTGKARSAIRRA
LREEDRARFVKLGQELARSAFEHVGRKATDKALEAAARNMRCNDRIDLLARLGSAEITGRDVVQAVYPELVPREGVKVDA
KRAVIGLSPGQSFERAFCCQPLPGERIVGITFKGRGVVVHAIDCDRMSVYEDQPDRWLDLRWHEGAHPAVYGATIELTLN
NDAGTLGRICTLIGETRANISDLQFVDRKPDYFLLLIYVELRDVAHLHSLMLTLEAESDVASVSRYRNMAHDKTETPA

Sequences:

>Translated_718_residues
MKDGDHLEISADDLVSLVLRYNPKTNQKLIRAAYDYGREMHADQLRHSGELYFNHPVAVAAILTEQNLDDATLITALLHD
TIEDTPASYSEVAARFGDEIAELVDGVTKLTNLQLNSSQTKQAENFRKLFMAMSKDLRVILVKLSDRLHNMRTIRAMKPE
KQAQKARETMDIYAPLAGRMGMQWMREELEDLAFRVLNPEGRASIIRRFINLQKETGDVIQRITGDMRLELDKAGVQAEV
FGRAKKPYSIWRKMQEKDQSFARLSDIYGFRIITDSEEDCYRALGAIHQRWRAVPGRFKDYISQPKSNGYRSIHTTVSGR
DGKRVEVQIRTVQMHDVAETGVAAHWSYRDGVRSNNPFAVDPAKWISTLTEQFDAEEDHEDFLEAVKLEMYSDQVFCFTP
KGEVVKLPRGATPIDFAFAIHTRIGMACVGAKVDHMRVPLWTRLKNGQSVEVITAEGQSPQATWLDIAVTGKARSAIRRA
LREEDRARFVKLGQELARSAFEHVGRKATDKALEAAARNMRCNDRIDLLARLGSAEITGRDVVQAVYPELVPREGVKVDA
KRAVIGLSPGQSFERAFCCQPLPGERIVGITFKGRGVVVHAIDCDRMSVYEDQPDRWLDLRWHEGAHPAVYGATIELTLN
NDAGTLGRICTLIGETRANISDLQFVDRKPDYFLLLIYVELRDVAHLHSLMLTLEAESDVASVSRYRNMAHDKTETPA
>Mature_718_residues
MKDGDHLEISADDLVSLVLRYNPKTNQKLIRAAYDYGREMHADQLRHSGELYFNHPVAVAAILTEQNLDDATLITALLHD
TIEDTPASYSEVAARFGDEIAELVDGVTKLTNLQLNSSQTKQAENFRKLFMAMSKDLRVILVKLSDRLHNMRTIRAMKPE
KQAQKARETMDIYAPLAGRMGMQWMREELEDLAFRVLNPEGRASIIRRFINLQKETGDVIQRITGDMRLELDKAGVQAEV
FGRAKKPYSIWRKMQEKDQSFARLSDIYGFRIITDSEEDCYRALGAIHQRWRAVPGRFKDYISQPKSNGYRSIHTTVSGR
DGKRVEVQIRTVQMHDVAETGVAAHWSYRDGVRSNNPFAVDPAKWISTLTEQFDAEEDHEDFLEAVKLEMYSDQVFCFTP
KGEVVKLPRGATPIDFAFAIHTRIGMACVGAKVDHMRVPLWTRLKNGQSVEVITAEGQSPQATWLDIAVTGKARSAIRRA
LREEDRARFVKLGQELARSAFEHVGRKATDKALEAAARNMRCNDRIDLLARLGSAEITGRDVVQAVYPELVPREGVKVDA
KRAVIGLSPGQSFERAFCCQPLPGERIVGITFKGRGVVVHAIDCDRMSVYEDQPDRWLDLRWHEGAHPAVYGATIELTLN
NDAGTLGRICTLIGETRANISDLQFVDRKPDYFLLLIYVELRDVAHLHSLMLTLEAESDVASVSRYRNMAHDKTETPA

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=144, Percent_Identity=36.1111111111111, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790082, Length=708, Percent_Identity=39.2655367231638, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1789147, Length=641, Percent_Identity=33.3853354134165, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25143118, Length=134, Percent_Identity=32.8358208955224, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24650996, Length=125, Percent_Identity=33.6, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 81140; Mature: 81140

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDGDHLEISADDLVSLVLRYNPKTNQKLIRAAYDYGREMHADQLRHSGELYFNHPVAVA
CCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCEEE
AILTEQNLDDATLITALLHDTIEDTPASYSEVAARFGDEIAELVDGVTKLTNLQLNSSQT
EEHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHH
KQAENFRKLFMAMSKDLRVILVKLSDRLHNMRTIRAMKPEKQAQKARETMDIYAPLAGRM
HHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GMQWMREELEDLAFRVLNPEGRASIIRRFINLQKETGDVIQRITGDMRLELDKAGVQAEV
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
FGRAKKPYSIWRKMQEKDQSFARLSDIYGFRIITDSEEDCYRALGAIHQRWRAVPGRFKD
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHH
YISQPKSNGYRSIHTTVSGRDGKRVEVQIRTVQMHDVAETGVAAHWSYRDGVRSNNPFAV
HHCCCCCCCCEEEEEEECCCCCCEEEEEEEEEEEHHHHHCCCEEEECHHCCCCCCCCEEE
DPAKWISTLTEQFDAEEDHEDFLEAVKLEMYSDQVFCFTPKGEVVKLPRGATPIDFAFAI
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCHHHHHHH
HTRIGMACVGAKVDHMRVPLWTRLKNGQSVEVITAEGQSPQATWLDIAVTGKARSAIRRA
HHHHHHHHHCCCCCCEECCHHHHCCCCCEEEEEEECCCCCCCEEEEEEEECHHHHHHHHH
LREEDRARFVKLGQELARSAFEHVGRKATDKALEAAARNMRCNDRIDLLARLGSAEITGR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHH
DVVQAVYPELVPREGVKVDAKRAVIGLSPGQSFERAFCCQPLPGERIVGITFKGRGVVVH
HHHHHHHHHHCCCCCCEEECCEEEEECCCCCCHHHHHCCCCCCCCEEEEEEEECCEEEEE
AIDCDRMSVYEDQPDRWLDLRWHEGAHPAVYGATIELTLNNDAGTLGRICTLIGETRANI
EECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCH
SDLQFVDRKPDYFLLLIYVELRDVAHLHSLMLTLEAESDVASVSRYRNMAHDKTETPA
HHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKDGDHLEISADDLVSLVLRYNPKTNQKLIRAAYDYGREMHADQLRHSGELYFNHPVAVA
CCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCEEE
AILTEQNLDDATLITALLHDTIEDTPASYSEVAARFGDEIAELVDGVTKLTNLQLNSSQT
EEHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHH
KQAENFRKLFMAMSKDLRVILVKLSDRLHNMRTIRAMKPEKQAQKARETMDIYAPLAGRM
HHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GMQWMREELEDLAFRVLNPEGRASIIRRFINLQKETGDVIQRITGDMRLELDKAGVQAEV
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
FGRAKKPYSIWRKMQEKDQSFARLSDIYGFRIITDSEEDCYRALGAIHQRWRAVPGRFKD
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHH
YISQPKSNGYRSIHTTVSGRDGKRVEVQIRTVQMHDVAETGVAAHWSYRDGVRSNNPFAV
HHCCCCCCCCEEEEEEECCCCCCEEEEEEEEEEEHHHHHCCCEEEECHHCCCCCCCCEEE
DPAKWISTLTEQFDAEEDHEDFLEAVKLEMYSDQVFCFTPKGEVVKLPRGATPIDFAFAI
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCHHHHHHH
HTRIGMACVGAKVDHMRVPLWTRLKNGQSVEVITAEGQSPQATWLDIAVTGKARSAIRRA
HHHHHHHHHCCCCCCEECCHHHHCCCCCEEEEEEECCCCCCCEEEEEEEECHHHHHHHHH
LREEDRARFVKLGQELARSAFEHVGRKATDKALEAAARNMRCNDRIDLLARLGSAEITGR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHH
DVVQAVYPELVPREGVKVDAKRAVIGLSPGQSFERAFCCQPLPGERIVGITFKGRGVVVH
HHHHHHHHHHCCCCCCEEECCEEEEECCCCCCHHHHHCCCCCCCCEEEEEEEECCEEEEE
AIDCDRMSVYEDQPDRWLDLRWHEGAHPAVYGATIELTLNNDAGTLGRICTLIGETRANI
EECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCH
SDLQFVDRKPDYFLLLIYVELRDVAHLHSLMLTLEAESDVASVSRYRNMAHDKTETPA
HHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA