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The map label for this gene is nikA [H]

Identifier: 110678615

GI number: 110678615

Start: 1252598

End: 1254259

Strand: Reverse

Name: nikA [H]

Synonym: RD1_1291

Alternate gene names: 110678615

Gene position: 1254259-1252598 (Counterclockwise)

Preceding gene: 110678616

Following gene: 110678614

Centisome position: 30.35

GC content: 59.99

Gene sequence:

>1662_bases
ATGACATTTCTGACAAGCACCGGCAAACCCTTGCCGAAGTCCATTCTGGACTCCGCTGCGAAGACCGGCAACGACGCCGT
AAGCCGACGCGAGTTTCTAGCGCTCGCGAGCGCTTTCGGTGCGACATCCGCCACCGCCTATGCAATGCTTGGCCTCAGCG
CGCCCAAAGCGCATGCAGCGGCGCACACGATGGGCGGCACGGTCCGCATTCAACAGGAAGTCCGCGCGCTCAAAGACCCG
CGCACCTATGACTGGAGTCAGATCGCGAACTTCAGCCGCGGCTGGCTCGAATACCTCGTCCATTACGAGAACGACGGCAC
CTTTACCGGCAAGCTGCTGGAAAGCTGGGAAATCAACGAAGACGCGACTGTTTACACCCTGAATGTGCGTCAGGGCGTCA
AGTGGAACGACGGCAGCGATTTCACTGCCGAAGACGTGGCACGCAACATCGCCGGCTGGTGCGACAAGAGCCTTGAGGGC
AATTCAATGGCAGGCCGCTTTGCGACCCTGATTGATCCCAATACGGATCAGGCCATCGACGGCGCAATCGAGGTCGTGGA
CAGCCACACGGTACGCCTCAATCTGCCCTCTGCGGATATCTCGCTGATCCCCGGCATGGCCGACTATCCGGCTGCGATTG
TTCCGGCGGGCTTCTCAGCGGAAACGATGCTCGACAACCCGGTGGGAACAGGGCCTTACCTGCCTGAATCGCTGGAAGTC
GGCGTGAAGGCCGTTCTGGTGCGCAACGAGGGGCACACCTGGTGGAACGCAGGCAATGGTGCCTGGATAGACCGGGTGGA
ATACATCGACTATGGCACGGACCCTTCCGCCTTTGTCGCGGCGGCAGAAGCCGAAGAGATCGACATGACCTACTCCATAG
AGGGTGATTTCATCGACATCTTTGACACACTTGACGGCTGGCAAAACAACGCCGTTGTGACAGGCGCCACGATCGTCATA
CGACCCAATCAGCAGGTCGAAGTCGACGGCATCAGGCCCTATGAAGACAAACGCGTCCGTCAGGCGATTTCGATGGCCGT
TGACAACGCGGTGCTGCTGGAACTGGGGTATGCCGGGCGGGGAGAGGTAGCCGAAAACCACCACGTTGGCCCCATGCATC
CCGAATACGCGGAATTGCCACCGCGCAAACATGATCCAGCCGCCGCCAAGGCGCTGATGGACGAAGCCGGCATGGGAGAT
TTCGAGCATGAATTGTTCTCGATTGATGATGCCTGGCGCAAGGACACAACAGACGCCGTGGCAGCCCAGCTGCGCGACGC
GGGCATCAATGTGAAACGCACCATCCTGCCCGGCTCAACCTTCTGGAACGACTGGACGAAGTACCCGTTCTCCTCCACCA
ACTGGAACCACCGCCCCCTTGGTGTGCAGATCTGGGCACTTGCCTATCGCTCCGGCGAGGCGTGGAACGAATTCGGTTGG
TCCAACGAAGAGTTCGACGCCATCCTCACCGAGGCGCTGGCAACGCCCGACCTCGAAGCGCGCCGTGCGCTGATGAAGAA
GGGTCAGGCGATCATTCAGGAAGAAGGTGTAACCATCCAGCCCTATTGGCGCTCGCTTTACAACCACACGCGCGAAGGAC
TGGTCGGCGGTGAGCACCACATCTCATTCGAGATCCGTCCGGAGCAGATCGCCTGGACGTAA

Upstream 100 bases:

>100_bases
CTCGGGGCGCGAAAGAAAGGCAGATCAACTGCATCATCTTGCCGTCACAGACATCGGCAATGGCACGCCACACGCGCGTG
TTCCAACAGAGAGGAAGAAC

Downstream 100 bases:

>100_bases
CCCATCCATCAAACAACGGGGCCATGCCATGAGTGTGGCCCCTTTTTTGTGATCGCGCGGAGCCGCAGGAGAAGCCCATG
GAAACCGCCTTGCTGCTCGC

Product: dipeptide ABC transporter, putative

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTFLTSTGKPLPKSILDSAAKTGNDAVSRREFLALASAFGATSATAYAMLGLSAPKAHAAAHTMGGTVRIQQEVRALKDP
RTYDWSQIANFSRGWLEYLVHYENDGTFTGKLLESWEINEDATVYTLNVRQGVKWNDGSDFTAEDVARNIAGWCDKSLEG
NSMAGRFATLIDPNTDQAIDGAIEVVDSHTVRLNLPSADISLIPGMADYPAAIVPAGFSAETMLDNPVGTGPYLPESLEV
GVKAVLVRNEGHTWWNAGNGAWIDRVEYIDYGTDPSAFVAAAEAEEIDMTYSIEGDFIDIFDTLDGWQNNAVVTGATIVI
RPNQQVEVDGIRPYEDKRVRQAISMAVDNAVLLELGYAGRGEVAENHHVGPMHPEYAELPPRKHDPAAAKALMDEAGMGD
FEHELFSIDDAWRKDTTDAVAAQLRDAGINVKRTILPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFGW
SNEEFDAILTEALATPDLEARRALMKKGQAIIQEEGVTIQPYWRSLYNHTREGLVGGEHHISFEIRPEQIAWT

Sequences:

>Translated_553_residues
MTFLTSTGKPLPKSILDSAAKTGNDAVSRREFLALASAFGATSATAYAMLGLSAPKAHAAAHTMGGTVRIQQEVRALKDP
RTYDWSQIANFSRGWLEYLVHYENDGTFTGKLLESWEINEDATVYTLNVRQGVKWNDGSDFTAEDVARNIAGWCDKSLEG
NSMAGRFATLIDPNTDQAIDGAIEVVDSHTVRLNLPSADISLIPGMADYPAAIVPAGFSAETMLDNPVGTGPYLPESLEV
GVKAVLVRNEGHTWWNAGNGAWIDRVEYIDYGTDPSAFVAAAEAEEIDMTYSIEGDFIDIFDTLDGWQNNAVVTGATIVI
RPNQQVEVDGIRPYEDKRVRQAISMAVDNAVLLELGYAGRGEVAENHHVGPMHPEYAELPPRKHDPAAAKALMDEAGMGD
FEHELFSIDDAWRKDTTDAVAAQLRDAGINVKRTILPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFGW
SNEEFDAILTEALATPDLEARRALMKKGQAIIQEEGVTIQPYWRSLYNHTREGLVGGEHHISFEIRPEQIAWT
>Mature_552_residues
TFLTSTGKPLPKSILDSAAKTGNDAVSRREFLALASAFGATSATAYAMLGLSAPKAHAAAHTMGGTVRIQQEVRALKDPR
TYDWSQIANFSRGWLEYLVHYENDGTFTGKLLESWEINEDATVYTLNVRQGVKWNDGSDFTAEDVARNIAGWCDKSLEGN
SMAGRFATLIDPNTDQAIDGAIEVVDSHTVRLNLPSADISLIPGMADYPAAIVPAGFSAETMLDNPVGTGPYLPESLEVG
VKAVLVRNEGHTWWNAGNGAWIDRVEYIDYGTDPSAFVAAAEAEEIDMTYSIEGDFIDIFDTLDGWQNNAVVTGATIVIR
PNQQVEVDGIRPYEDKRVRQAISMAVDNAVLLELGYAGRGEVAENHHVGPMHPEYAELPPRKHDPAAAKALMDEAGMGDF
EHELFSIDDAWRKDTTDAVAAQLRDAGINVKRTILPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFGWS
NEEFDAILTEALATPDLEARRALMKKGQAIIQEEGVTIQPYWRSLYNHTREGLVGGEHHISFEIRPEQIAWT

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=366, Percent_Identity=28.6885245901639, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789966, Length=511, Percent_Identity=22.1135029354207, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787495, Length=480, Percent_Identity=22.7083333333333, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60981; Mature: 60850

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLTSTGKPLPKSILDSAAKTGNDAVSRREFLALASAFGATSATAYAMLGLSAPKAHAA
CCEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCHHHH
AHTMGGTVRIQQEVRALKDPRTYDWSQIANFSRGWLEYLVHYENDGTFTGKLLESWEINE
HHHCCCEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCEEEEEEHHCCCCCC
DATVYTLNVRQGVKWNDGSDFTAEDVARNIAGWCDKSLEGNSMAGRFATLIDPNTDQAID
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHH
GAIEVVDSHTVRLNLPSADISLIPGMADYPAAIVPAGFSAETMLDNPVGTGPYLPESLEV
HHHEEECCCEEEEECCCCCCEECCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCCHHHCC
GVKAVLVRNEGHTWWNAGNGAWIDRVEYIDYGTDPSAFVAAAEAEEIDMTYSIEGDFIDI
CEEEEEEECCCCCEEECCCCCEEEEEEEEECCCCCCCEEEECCCCCCEEEEEECCCEEEE
FDTLDGWQNNAVVTGATIVIRPNQQVEVDGIRPYEDKRVRQAISMAVDNAVLLELGYAGR
HHHHCCCCCCEEEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCC
GEVAENHHVGPMHPEYAELPPRKHDPAAAKALMDEAGMGDFEHELFSIDDAWRKDTTDAV
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCHHHH
AAQLRDAGINVKRTILPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFGW
HHHHHHCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHCCC
SNEEFDAILTEALATPDLEARRALMKKGQAIIQEEGVTIQPYWRSLYNHTREGLVGGEHH
CCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCEECHHHHHHHHHHHHCCCCCCEE
ISFEIRPEQIAWT
EEEEECCCCCCCC
>Mature Secondary Structure 
TFLTSTGKPLPKSILDSAAKTGNDAVSRREFLALASAFGATSATAYAMLGLSAPKAHAA
CEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCHHHH
AHTMGGTVRIQQEVRALKDPRTYDWSQIANFSRGWLEYLVHYENDGTFTGKLLESWEINE
HHHCCCEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCEEEEEEHHCCCCCC
DATVYTLNVRQGVKWNDGSDFTAEDVARNIAGWCDKSLEGNSMAGRFATLIDPNTDQAID
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHH
GAIEVVDSHTVRLNLPSADISLIPGMADYPAAIVPAGFSAETMLDNPVGTGPYLPESLEV
HHHEEECCCEEEEECCCCCCEECCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCCHHHCC
GVKAVLVRNEGHTWWNAGNGAWIDRVEYIDYGTDPSAFVAAAEAEEIDMTYSIEGDFIDI
CEEEEEEECCCCCEEECCCCCEEEEEEEEECCCCCCCEEEECCCCCCEEEEEECCCEEEE
FDTLDGWQNNAVVTGATIVIRPNQQVEVDGIRPYEDKRVRQAISMAVDNAVLLELGYAGR
HHHHCCCCCCEEEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCC
GEVAENHHVGPMHPEYAELPPRKHDPAAAKALMDEAGMGDFEHELFSIDDAWRKDTTDAV
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCHHHH
AAQLRDAGINVKRTILPGSTFWNDWTKYPFSSTNWNHRPLGVQIWALAYRSGEAWNEFGW
HHHHHHCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHCCC
SNEEFDAILTEALATPDLEARRALMKKGQAIIQEEGVTIQPYWRSLYNHTREGLVGGEHH
CCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHCCCEECHHHHHHHHHHHHCCCCCCEE
ISFEIRPEQIAWT
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]