Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 110678547

GI number: 110678547

Start: 1184266

End: 1185591

Strand: Direct

Name: gltX

Synonym: RD1_1219

Alternate gene names: 110678547

Gene position: 1184266-1185591 (Clockwise)

Preceding gene: 110678544

Following gene: 110678548

Centisome position: 28.65

GC content: 61.39

Gene sequence:

>1326_bases
ATGACCACCACCCGTTTCGCCCCGTCGCCCACCGGCTACATCCATATCGGTAACCTGCGCACGGCTTTGATGAACTACCT
GATCGCGCGCAAGGCGGGCGGTACGTTCATCCTGCGCATTGACGATACAGACCCCGAAAGGTCCAAAGAGGAATATGTCG
ATGGCATCAAGCAAGACCTTGAATGGCTCGGCTTGCATTGGGACCGCATCGAGCGTCAGTCGCTGCGCCTTGATCGTTAT
GCGCAGGCCGCTGACAAACTGCGGGACATGGGGCGGTTTTACGAGGCTTTCGAGACACCGACCGAACTGGACCTCAAGCG
CAAGAAACAACTGAACATGGGCAAACCGCCGGTCTATGACCGGGCTGCACTGGAGTTGTCGGATGCTGAAAAGGACGCCT
TGCGCGCCGAACGCGGTGCGGGTGTTTGGCGGTTCAAGCTTGAGCGGGAACGCATTGAATGGGCCGATGGCATTCTGGGC
GACATTTCGATTGATGCGGCCTCCGTCTCCGATCCGGTGCTTATTCGCGGGGATGGTCAGATCCTCTATACGCTGGCCTC
CGTGGTGGATGACACGGAGATGGGTGTGACGAATGTGGTGCGCGGCTCGGATCATGTAACCAATACCGCGACACAAATTC
AGATCATCAACGCGCTAGGCGGCACGGTTCCCGACTTCGCGCATCACTCGCTGCTGACCGGCCCGCAGGGCGAGGCGTTG
TCAAAACGTCTGGGCACGCTGGCCCTGCGCGATCTGCGCGAAAACGGTGTGCAGCCCGCAGCCTTGCTGAGCCTGATGGC
GCGGCTTGGTTCCAGCGACCCGGTCGAGTTGCAAACCGACATGGCCGACCTGATCGAGGGGTTTGACATCTCGCGCTTTG
GCTCCGCACCGACGAAATTCGATGCCGATGATCTTTATCCGCTGACCGCGCGCTATCTGGCGGGTCTGCCTCTGGCCGAT
GTGGCGGGTGATCTGTCCGCAGCGGGCGTACCCGATGACATGGCGGCACAATTCTGGGATGTGACGCGCGAGAATATCAC
CACGCTCAAGGATATTGGCCCGTGGTGGACCCTGATGCGCGATGGTGCGGAGCCGCAGATCGACGACGAAGACCGCGAAT
TTGTCGCCGAGGCGCTGGCGCTTTTGCCGGACGGCCCCTTTGATGCGACGACCTGGGGCACATGGACCGCCGCCGTCAAG
GAAAAGACCGGGCGCAAGGGGCGCGCATTGTTCATGCCGCTGCGCAAGGCCCTGACAGGTCAGAGCCACGGGCCGGACAT
GTCGGGGCTGATGCCGCTGTTGCAGGTCGTCAAGGCCCGCCGATGA

Upstream 100 bases:

>100_bases
GTCCTTCACGATTTCGCGCGAAAGTTGACCAAGGTGCTTGATTGCGCTGCTGGACAATTACACGACCCTGTGACAACACC
GCGCCAACAGGAGACAGCAA

Downstream 100 bases:

>100_bases
GCTGCGCGCGCCCGGTTCATGGCTGAGGCGCAGGCGAAATTCCTGACGGGGAACCTGTTTCGCCATGTTTCAGTGATGTC
ACTGACCTCATCCGTGGGGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTTTRFAPSPTGYIHIGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDGIKQDLEWLGLHWDRIERQSLRLDRY
AQAADKLRDMGRFYEAFETPTELDLKRKKQLNMGKPPVYDRAALELSDAEKDALRAERGAGVWRFKLERERIEWADGILG
DISIDAASVSDPVLIRGDGQILYTLASVVDDTEMGVTNVVRGSDHVTNTATQIQIINALGGTVPDFAHHSLLTGPQGEAL
SKRLGTLALRDLRENGVQPAALLSLMARLGSSDPVELQTDMADLIEGFDISRFGSAPTKFDADDLYPLTARYLAGLPLAD
VAGDLSAAGVPDDMAAQFWDVTRENITTLKDIGPWWTLMRDGAEPQIDDEDREFVAEALALLPDGPFDATTWGTWTAAVK
EKTGRKGRALFMPLRKALTGQSHGPDMSGLMPLLQVVKARR

Sequences:

>Translated_441_residues
MTTTRFAPSPTGYIHIGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDGIKQDLEWLGLHWDRIERQSLRLDRY
AQAADKLRDMGRFYEAFETPTELDLKRKKQLNMGKPPVYDRAALELSDAEKDALRAERGAGVWRFKLERERIEWADGILG
DISIDAASVSDPVLIRGDGQILYTLASVVDDTEMGVTNVVRGSDHVTNTATQIQIINALGGTVPDFAHHSLLTGPQGEAL
SKRLGTLALRDLRENGVQPAALLSLMARLGSSDPVELQTDMADLIEGFDISRFGSAPTKFDADDLYPLTARYLAGLPLAD
VAGDLSAAGVPDDMAAQFWDVTRENITTLKDIGPWWTLMRDGAEPQIDDEDREFVAEALALLPDGPFDATTWGTWTAAVK
EKTGRKGRALFMPLRKALTGQSHGPDMSGLMPLLQVVKARR
>Mature_440_residues
TTTRFAPSPTGYIHIGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDGIKQDLEWLGLHWDRIERQSLRLDRYA
QAADKLRDMGRFYEAFETPTELDLKRKKQLNMGKPPVYDRAALELSDAEKDALRAERGAGVWRFKLERERIEWADGILGD
ISIDAASVSDPVLIRGDGQILYTLASVVDDTEMGVTNVVRGSDHVTNTATQIQIINALGGTVPDFAHHSLLTGPQGEALS
KRLGTLALRDLRENGVQPAALLSLMARLGSSDPVELQTDMADLIEGFDISRFGSAPTKFDADDLYPLTARYLAGLPLADV
AGDLSAAGVPDDMAAQFWDVTRENITTLKDIGPWWTLMRDGAEPQIDDEDREFVAEALALLPDGPFDATTWGTWTAAVKE
KTGRKGRALFMPLRKALTGQSHGPDMSGLMPLLQVVKARR

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=313, Percent_Identity=36.1022364217252, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1788743, Length=347, Percent_Identity=35.7348703170029, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=35.2226720647773, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1786895, Length=177, Percent_Identity=32.7683615819209, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71993394, Length=319, Percent_Identity=37.9310344827586, Blast_Score=181, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=326, Percent_Identity=33.7423312883436, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321192, Length=103, Percent_Identity=40.7766990291262, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=125, Percent_Identity=34.4, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=467, Percent_Identity=31.4775160599572, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_ROSDO (Q16AX5)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681554.1
- ProteinModelPortal:   Q16AX5
- SMR:   Q16AX5
- STRING:   Q16AX5
- GeneID:   4197467
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1219
- NMPDR:   fig|375451.6.peg.1138
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   RSKQEYI
- ProtClustDB:   PRK12558
- BioCyc:   RDEN375451:RD1_1219-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 48694; Mature: 48563

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS00166 ENOYL_COA_HYDRATASE ; PS00678 WD_REPEATS_1

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTRFAPSPTGYIHIGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDGIKQDL
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EWLGLHWDRIERQSLRLDRYAQAADKLRDMGRFYEAFETPTELDLKRKKQLNMGKPPVYD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
RAALELSDAEKDALRAERGAGVWRFKLERERIEWADGILGDISIDAASVSDPVLIRGDGQ
HHHHHHCCCHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCEECCCCCCCCEEEECCCH
ILYTLASVVDDTEMGVTNVVRGSDHVTNTATQIQIINALGGTVPDFAHHSLLTGPQGEAL
HHHHHHHHHCCHHCCHHHHHCCCCCCCCCHHHEEEHHHHCCCCCHHHHHHHCCCCCCHHH
SKRLGTLALRDLRENGVQPAALLSLMARLGSSDPVELQTDMADLIEGFDISRFGSAPTKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCC
DADDLYPLTARYLAGLPLADVAGDLSAAGVPDDMAAQFWDVTRENITTLKDIGPWWTLMR
CCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DGAEPQIDDEDREFVAEALALLPDGPFDATTWGTWTAAVKEKTGRKGRALFMPLRKALTG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
QSHGPDMSGLMPLLQVVKARR
CCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTTRFAPSPTGYIHIGNLRTALMNYLIARKAGGTFILRIDDTDPERSKEEYVDGIKQDL
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EWLGLHWDRIERQSLRLDRYAQAADKLRDMGRFYEAFETPTELDLKRKKQLNMGKPPVYD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
RAALELSDAEKDALRAERGAGVWRFKLERERIEWADGILGDISIDAASVSDPVLIRGDGQ
HHHHHHCCCHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCEECCCCCCCCEEEECCCH
ILYTLASVVDDTEMGVTNVVRGSDHVTNTATQIQIINALGGTVPDFAHHSLLTGPQGEAL
HHHHHHHHHCCHHCCHHHHHCCCCCCCCCHHHEEEHHHHCCCCCHHHHHHHCCCCCCHHH
SKRLGTLALRDLRENGVQPAALLSLMARLGSSDPVELQTDMADLIEGFDISRFGSAPTKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCC
DADDLYPLTARYLAGLPLADVAGDLSAAGVPDDMAAQFWDVTRENITTLKDIGPWWTLMR
CCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DGAEPQIDDEDREFVAEALALLPDGPFDATTWGTWTAAVKEKTGRKGRALFMPLRKALTG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
QSHGPDMSGLMPLLQVVKARR
CCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA