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The map label for this gene is gshB [H]

Identifier: 110678521

GI number: 110678521

Start: 1156204

End: 1157145

Strand: Direct

Name: gshB [H]

Synonym: RD1_1192

Alternate gene names: 110678521

Gene position: 1156204-1157145 (Clockwise)

Preceding gene: 110678520

Following gene: 110678526

Centisome position: 27.97

GC content: 58.7

Gene sequence:

>942_bases
ATGAAAATCGCCTTCCAGATGGATCCAATTGATGCGGTTGACATCAACGCAGACAGCTCGTTCCGCCTTGCCGAAGAGGC
GCAGGCGCGCGGCCATACCCTGTTTTTCTATACGCCCGATCATCTGGCCTATCAGGAAGGGCGCATCACCGCCAAGGGAC
AGGACCTGCAGGTCCAGCGCGTGCAGGGCGCACATGCAACGCTGGGTGCCATGCGCGAGGTGAACCTCGCGGAATTCGAT
GTGGTCTGGCTGCGGCAGGACCCGCCTTTTGACATGCATTACATTACATCGACCCACCTGCTGGATCGTTTGGCCAGCAC
GACGCTGGTGGTGAATGACCCGTTCTGGGTGCGCAACTATCCCGAAAAACTGTTGGTGCTGGATTTCCCGGACCTGACCC
CGCCGACCACGATCGCGCGCGATCTGGACACGATCAAGGCTTTCAAGGCAAAGCACGGCGATGTCATTCTCAAACCGCTC
TACGGGAACGGCGGTGCGGGCGTTTTCCGTCTGGATGCGAACGACCGGAACCTCACGTCGTTGCACGAGCTTTTTACCGG
ATTTTCGCGCGAACCGTTGATTGTGCAGAAATTCCTGCCGGATGTCTCAAACGGGGACAAACGCGTGATCCTCGTCGATG
GAGAACCGGTTGGGGCCATCAACCGTGTGCCCGCCGCTGGTGAGACGCGTTCCAACATGCATGTGGGCGGGCGTCCTGAA
AAGGTGGGACTGAGCGCGCGTGACAAGGAAATATGCGCCGCCATCGGCCCGCTTTTGAAGGAAAAGGGGCAGGTCTTCGT
CGGCATAGACGTGATCGGCGACTACCTGACCGAGATTAATGTGACGTCCCCCACGGGCATTCAGGAACTTGAACGATTTG
ATCAGGTGAACATCGCGGCACACATCTGGCAGGCCATTGAAGACAGGGCAGCGGCGCGCTGA

Upstream 100 bases:

>100_bases
GTGCAGTACCCAACGCATTGACATCTTTGATGTCAGGCACCGTTGGGTGATCCAGGTTTTTCGCACAACGCATTAAGCGG
CATAAACGCAAGGAGCTGCC

Downstream 100 bases:

>100_bases
CCCTCTAGGCATTGATGCGGTAGCTGATCGCCTCAGCCACATGTGGCTTGCGCACGCCTTCCGAATGATCGAGATCGGCA
ATCGTGCGCGCCACCCGCAT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKIAFQMDPIDAVDINADSSFRLAEEAQARGHTLFFYTPDHLAYQEGRITAKGQDLQVQRVQGAHATLGAMREVNLAEFD
VVWLRQDPPFDMHYITSTHLLDRLASTTLVVNDPFWVRNYPEKLLVLDFPDLTPPTTIARDLDTIKAFKAKHGDVILKPL
YGNGGAGVFRLDANDRNLTSLHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
KVGLSARDKEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFDQVNIAAHIWQAIEDRAAAR

Sequences:

>Translated_313_residues
MKIAFQMDPIDAVDINADSSFRLAEEAQARGHTLFFYTPDHLAYQEGRITAKGQDLQVQRVQGAHATLGAMREVNLAEFD
VVWLRQDPPFDMHYITSTHLLDRLASTTLVVNDPFWVRNYPEKLLVLDFPDLTPPTTIARDLDTIKAFKAKHGDVILKPL
YGNGGAGVFRLDANDRNLTSLHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
KVGLSARDKEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFDQVNIAAHIWQAIEDRAAAR
>Mature_313_residues
MKIAFQMDPIDAVDINADSSFRLAEEAQARGHTLFFYTPDHLAYQEGRITAKGQDLQVQRVQGAHATLGAMREVNLAEFD
VVWLRQDPPFDMHYITSTHLLDRLASTTLVVNDPFWVRNYPEKLLVLDFPDLTPPTTIARDLDTIKAFKAKHGDVILKPL
YGNGGAGVFRLDANDRNLTSLHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
KVGLSARDKEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFDQVNIAAHIWQAIEDRAAAR

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=311, Percent_Identity=43.4083601286174, Blast_Score=243, Evalue=9e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34692; Mature: 34692

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFQMDPIDAVDINADSSFRLAEEAQARGHTLFFYTPDHLAYQEGRITAKGQDLQVQR
CEEEEECCCCCEEEECCCCCCEEHHHHHHCCCEEEEECCCHHEEECCEEEECCCCEEEEE
VQGAHATLGAMREVNLAEFDVVWLRQDPPFDMHYITSTHLLDRLASTTLVVNDPFWVRNY
ECCCHHHHHHHHCCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHCEEEECCCHHCCCC
PEKLLVLDFPDLTPPTTIARDLDTIKAFKAKHGDVILKPLYGNGGAGVFRLDANDRNLTS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCHHH
LHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
HHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
KVGLSARDKEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFDQVNIAA
CCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHH
HIWQAIEDRAAAR
HHHHHHHHHHCCC
>Mature Secondary Structure
MKIAFQMDPIDAVDINADSSFRLAEEAQARGHTLFFYTPDHLAYQEGRITAKGQDLQVQR
CEEEEECCCCCEEEECCCCCCEEHHHHHHCCCEEEEECCCHHEEECCEEEECCCCEEEEE
VQGAHATLGAMREVNLAEFDVVWLRQDPPFDMHYITSTHLLDRLASTTLVVNDPFWVRNY
ECCCHHHHHHHHCCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHCEEEECCCHHCCCC
PEKLLVLDFPDLTPPTTIARDLDTIKAFKAKHGDVILKPLYGNGGAGVFRLDANDRNLTS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCHHH
LHELFTGFSREPLIVQKFLPDVSNGDKRVILVDGEPVGAINRVPAAGETRSNMHVGGRPE
HHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
KVGLSARDKEICAAIGPLLKEKGQVFVGIDVIGDYLTEINVTSPTGIQELERFDQVNIAA
CCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHH
HIWQAIEDRAAAR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]