Click here to switch to the map view.

The map label for this gene is rpoH2 [H]

Identifier: 110678526

GI number: 110678526

Start: 1160669

End: 1161547

Strand: Direct

Name: rpoH2 [H]

Synonym: RD1_1197

Alternate gene names: 110678526

Gene position: 1160669-1161547 (Clockwise)

Preceding gene: 110678521

Following gene: 110678527

Centisome position: 28.08

GC content: 56.66

Gene sequence:

>879_bases
ATGGCTTTGGACTCAACACGGGATATGGCTTTGTCGCGCAAGGCGATGAAAGCGGAACTGCTGGATGCGGAAACCGAGCT
TGAACTCGCTTACGCGTGGCGGGACGAGCGTTGCGAGAAATCATTGCACCGACTGATCACTGCCTACATGCGCCTCGCAA
TCTCCATGGCATCCAAATTCAAACGCTACGGCGCGCCCATGAATGACCTGATTCAGGAAGCAGGTCTGGGGCTCATGAAG
GCTGCGGATAAGTTTGATCCGGACAGAGGCGTGCGCTTTTCAACTTATGCGGTGTGGTGGATCAAAGCCTCCATTCAGGA
CTACGTGATGCGCAATTGGTCCATGGTGCGTACCGGGTCGACCTCATCGCAAAAATCGCTCTTCTTCAACATGCGCCGCG
TGCAGGCCAGACTGGAGCGTGAAAGCGCTGCATCGGGCGAACCTTTGGACCGTCATCAGCTTCGGCAGATGATTTCAACC
GAGATCGGCGTTCCGTTGCACGATGTCGAGATGATGGAAGGCCGCCTTTCGGGTTCCGACTATTCGTTGAATGCGACGCA
ATCGACGGAGGATGAGGGGCGCGAGTGGATTGACGCGCTGGAGGACGACTCCGCGCAGGCGGCCGAAACGGTTGAGCATC
ACCACGATACCGAACAGCTGCGTGCATGGTTGCTGCAAGCCATGAATGACCTGAACGAACGTGAGCGTTTCATCGTTCGC
GAGCGCAAGTTGCGGGATGCACCGCGCACCTTGGAAAGCCTCGGTCAGGAGTTGAACCTCAGCAAGGAACGCGTGCGCCA
GCTTGAGGCCGCAGCCTTCGGGAAAATGCGCAAGAGCCTTGAAAATCAATCCCGCGAGGTTTTGTATTTCCTCGACTGA

Upstream 100 bases:

>100_bases
CATTTTATAAACCTTTGAAGTGAACCAATCGCTTCTAGACTGCGTATCTATTTCAAATGTGCACGGCAAGGTGTGCTTCA
TCTGACTGGGAGATGTCGCT

Downstream 100 bases:

>100_bases
TGCTCCGTAACGCACTTCTGGCAAGTTGTTGTGGCGTTGTCTTGTGCTTCGCCGCTTGGGCGGACGTTATTGTACCGCCG
AGCGAAGCCTTCGAAGCGGA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MALDSTRDMALSRKAMKAELLDAETELELAYAWRDERCEKSLHRLITAYMRLAISMASKFKRYGAPMNDLIQEAGLGLMK
AADKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGSTSSQKSLFFNMRRVQARLERESAASGEPLDRHQLRQMIST
EIGVPLHDVEMMEGRLSGSDYSLNATQSTEDEGREWIDALEDDSAQAAETVEHHHDTEQLRAWLLQAMNDLNERERFIVR
ERKLRDAPRTLESLGQELNLSKERVRQLEAAAFGKMRKSLENQSREVLYFLD

Sequences:

>Translated_292_residues
MALDSTRDMALSRKAMKAELLDAETELELAYAWRDERCEKSLHRLITAYMRLAISMASKFKRYGAPMNDLIQEAGLGLMK
AADKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGSTSSQKSLFFNMRRVQARLERESAASGEPLDRHQLRQMIST
EIGVPLHDVEMMEGRLSGSDYSLNATQSTEDEGREWIDALEDDSAQAAETVEHHHDTEQLRAWLLQAMNDLNERERFIVR
ERKLRDAPRTLESLGQELNLSKERVRQLEAAAFGKMRKSLENQSREVLYFLD
>Mature_291_residues
ALDSTRDMALSRKAMKAELLDAETELELAYAWRDERCEKSLHRLITAYMRLAISMASKFKRYGAPMNDLIQEAGLGLMKA
ADKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGSTSSQKSLFFNMRRVQARLERESAASGEPLDRHQLRQMISTE
IGVPLHDVEMMEGRLSGSDYSLNATQSTEDEGREWIDALEDDSAQAAETVEHHHDTEQLRAWLLQAMNDLNERERFIVRE
RKLRDAPRTLESLGQELNLSKERVRQLEAAAFGKMRKSLENQSREVLYFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=240, Percent_Identity=37.0833333333333, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=29.5620437956204, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33775; Mature: 33643

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDSTRDMALSRKAMKAELLDAETELELAYAWRDERCEKSLHRLITAYMRLAISMASKF
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYGAPMNDLIQEAGLGLMKAADKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGS
HHHCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEHEEEEHHHHHHHHHHHHCCHHEECCC
TSSQKSLFFNMRRVQARLERESAASGEPLDRHQLRQMISTEIGVPLHDVEMMEGRLSGSD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
YSLNATQSTEDEGREWIDALEDDSAQAAETVEHHHDTEQLRAWLLQAMNDLNERERFIVR
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERKLRDAPRTLESLGQELNLSKERVRQLEAAAFGKMRKSLENQSREVLYFLD
HHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
ALDSTRDMALSRKAMKAELLDAETELELAYAWRDERCEKSLHRLITAYMRLAISMASKF
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYGAPMNDLIQEAGLGLMKAADKFDPDRGVRFSTYAVWWIKASIQDYVMRNWSMVRTGS
HHHCCCHHHHHHHHCCHHHHHHHHCCCCCCCEEEHEEEEHHHHHHHHHHHHCCHHEECCC
TSSQKSLFFNMRRVQARLERESAASGEPLDRHQLRQMISTEIGVPLHDVEMMEGRLSGSD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
YSLNATQSTEDEGREWIDALEDDSAQAAETVEHHHDTEQLRAWLLQAMNDLNERERFIVR
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERKLRDAPRTLESLGQELNLSKERVRQLEAAAFGKMRKSLENQSREVLYFLD
HHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]