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The map label for this gene is aspS

Identifier: 110678506

GI number: 110678506

Start: 1140739

End: 1142514

Strand: Direct

Name: aspS

Synonym: RD1_1176

Alternate gene names: 110678506

Gene position: 1140739-1142514 (Clockwise)

Preceding gene: 110678500

Following gene: 110678507

Centisome position: 27.6

GC content: 56.93

Gene sequence:

>1776_bases
ATGCACATGTACCGCAGTCACACTTGCGCAGATCTGAGCGCGCAGGACGTAGGTAAAACGGTGCGGCTGTCTGGTTGGGT
GCATCGTGTGCGCGACCATGGCGGGGTGCTTTTTATTGATCTGCGTGACCACTACGGTATCACGCAGGTTCTGTGCGATC
CCGACAGCCCGGTTTTTGAACAGATGGAACAGGTGCGCGCCGAATGGTGTATCCGCGTGGATGGAGTCGTCAAAGCCCGG
GATGCCAGTTTGATTAACCCCAAGATATCGACAGGTGAGATCGAGTTGTTCGTCAAGGATCTCGAAGTGCTTGGGGCGTC
GGAGGAACTGCCGCTTCAGGTTTTTGGCGATCAGGAGTACCCCGAAGAAACACGCCTGAAGTATCGGTATCTGGATCTGC
GTCGCGCCAACATGCAGGCGAACATGAAACTGCGCTCGGATGTGGTTGCCTCACTCCGCCAGCAGATGTGGAAAGCCGAT
TTTCGCGAATTCCAAACACCGATCATAACCGCCTCCAGCCCGGAAGGCGCGCGCGATTTCCTTGTGCCATCGCGTTTGCA
TCCGGGCCGGTTTTATGCGCTGCCGCAAGCGCCGCAGCAGTTCAAACAGCTTTTGATGGTATCAGGTTTTGACAAATACT
TTCAGATCGCGCCGTGTTTCAGAGATGAAGACCCGCGCGCCGATCGCTCGCCGACCGATTTCTATCAGCTCGATATCGAA
ATGAGTTTCGTGACACAGCAGGACGTCTTTGATGTCATCCAACCTGTCTTGACGCAGGTTTTCGAGCAGTTCGGCAATGG
GAAGGCCGTTGATCAGGAGTGGCCACAGATTTCCTATAAGGACGCCGCGCTCTGGTATGGATCGGACAAGCCCGATCTGC
GCAACCCGATCAAGATGCAATCGGTTTCCGAGCATTTCGCAGGATCCGGTTTTGCCATTTTCGCGAACCTTCTGGAACAG
GAAGGCACAGAGATCAGGGCGATCCCCGCGCCGGGTGGCGGCAGCCGAAAGTTCTGTGACCGCATGAATGCCTTTGCGCA
GAAAGAAGGTCTGCCGGGCATGGGGTATATTTTCTGGCGCGATCAGGGCGCTGGCATGGAAGCCGCAGGGCCGCTTGCGA
AGAACATCGGCCCGGAGCGTACCGAGGCGATCCGTCAGCAACTGGGCCTTGGCGTCGGGGATGCGGCGTTTTTCCTCGGC
GGCAAGCCCAAGGCCTTTGAAACCGTCGCGGGCAAAGCCAGAAACCACATCGGTATGGAACTGGGCCTTACGGATCAGAA
TCGGTTCGCCTTTGCGTGGATCGTCGATTTCCCGATTTATGAGAAAGACAGCGAAACCGGCCGGATTGATTTCGAACACA
ACCCGTTTTCGATGCCTCAGGGCGGTCTGGAGGCCTTGCAGGGCGATCCGCTCAGCGTGGTGGGGTATCAGTATGATCTG
TCGTGCAACGGATACGAGCTGGTGTCGGGCGCCATTCGAAACCACAAACCCGAGATCATGTTCAAGGCGTTTGAAATCGC
GGGCTATGGCGAAGATGAGGTGCGCAAACGTTTCGGTGGCATGGTCAATGCCTTCCAATATGGCGCGCCCCCGCATGGTG
GTTGTGCCGCTGGGATCGACCGGATCGTCATGCTGCTGGCCGAAGAAGCCAACATTCGCGAGGTCATCCTCTTTCCGATG
AACCAGCGTGCCGAAGACCTGATGATGTCCGCGCCGTCTGAACCGGAAAGCGAACAGCTGATGGAGCTGGGCTTGCGGGT
CATTCCCAAGGACTGA

Upstream 100 bases:

>100_bases
CTGCGCCGCAGCGTCAAAAGATCAACGCGCATGGCTTGACTTCCTGATATGCAGAAGGTGTATCTGGCGGCGAAATCCAC
TCCCGCAAAGGAATAGTCTC

Downstream 100 bases:

>100_bases
CCCCATAACGCCCCGACTTTTAACGGGAGGCTTGCAGATGCCGCGCAGTACCAGTCAATCGCCGGTTCTCGGTATGCCTG
TGCCGAATTGGTCAGTGCCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MHMYRSHTCADLSAQDVGKTVRLSGWVHRVRDHGGVLFIDLRDHYGITQVLCDPDSPVFEQMEQVRAEWCIRVDGVVKAR
DASLINPKISTGEIELFVKDLEVLGASEELPLQVFGDQEYPEETRLKYRYLDLRRANMQANMKLRSDVVASLRQQMWKAD
FREFQTPIITASSPEGARDFLVPSRLHPGRFYALPQAPQQFKQLLMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDIE
MSFVTQQDVFDVIQPVLTQVFEQFGNGKAVDQEWPQISYKDAALWYGSDKPDLRNPIKMQSVSEHFAGSGFAIFANLLEQ
EGTEIRAIPAPGGGSRKFCDRMNAFAQKEGLPGMGYIFWRDQGAGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFFLG
GKPKAFETVAGKARNHIGMELGLTDQNRFAFAWIVDFPIYEKDSETGRIDFEHNPFSMPQGGLEALQGDPLSVVGYQYDL
SCNGYELVSGAIRNHKPEIMFKAFEIAGYGEDEVRKRFGGMVNAFQYGAPPHGGCAAGIDRIVMLLAEEANIREVILFPM
NQRAEDLMMSAPSEPESEQLMELGLRVIPKD

Sequences:

>Translated_591_residues
MHMYRSHTCADLSAQDVGKTVRLSGWVHRVRDHGGVLFIDLRDHYGITQVLCDPDSPVFEQMEQVRAEWCIRVDGVVKAR
DASLINPKISTGEIELFVKDLEVLGASEELPLQVFGDQEYPEETRLKYRYLDLRRANMQANMKLRSDVVASLRQQMWKAD
FREFQTPIITASSPEGARDFLVPSRLHPGRFYALPQAPQQFKQLLMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDIE
MSFVTQQDVFDVIQPVLTQVFEQFGNGKAVDQEWPQISYKDAALWYGSDKPDLRNPIKMQSVSEHFAGSGFAIFANLLEQ
EGTEIRAIPAPGGGSRKFCDRMNAFAQKEGLPGMGYIFWRDQGAGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFFLG
GKPKAFETVAGKARNHIGMELGLTDQNRFAFAWIVDFPIYEKDSETGRIDFEHNPFSMPQGGLEALQGDPLSVVGYQYDL
SCNGYELVSGAIRNHKPEIMFKAFEIAGYGEDEVRKRFGGMVNAFQYGAPPHGGCAAGIDRIVMLLAEEANIREVILFPM
NQRAEDLMMSAPSEPESEQLMELGLRVIPKD
>Mature_591_residues
MHMYRSHTCADLSAQDVGKTVRLSGWVHRVRDHGGVLFIDLRDHYGITQVLCDPDSPVFEQMEQVRAEWCIRVDGVVKAR
DASLINPKISTGEIELFVKDLEVLGASEELPLQVFGDQEYPEETRLKYRYLDLRRANMQANMKLRSDVVASLRQQMWKAD
FREFQTPIITASSPEGARDFLVPSRLHPGRFYALPQAPQQFKQLLMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDIE
MSFVTQQDVFDVIQPVLTQVFEQFGNGKAVDQEWPQISYKDAALWYGSDKPDLRNPIKMQSVSEHFAGSGFAIFANLLEQ
EGTEIRAIPAPGGGSRKFCDRMNAFAQKEGLPGMGYIFWRDQGAGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFFLG
GKPKAFETVAGKARNHIGMELGLTDQNRFAFAWIVDFPIYEKDSETGRIDFEHNPFSMPQGGLEALQGDPLSVVGYQYDL
SCNGYELVSGAIRNHKPEIMFKAFEIAGYGEDEVRKRFGGMVNAFQYGAPPHGGCAAGIDRIVMLLAEEANIREVILFPM
NQRAEDLMMSAPSEPESEQLMELGLRVIPKD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=35.0993377483444, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI45439306, Length=291, Percent_Identity=29.553264604811, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=24.5059288537549, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=41.2162162162162, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1789256, Length=311, Percent_Identity=27.6527331189711, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=604, Percent_Identity=32.4503311258278, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17551876, Length=279, Percent_Identity=28.673835125448, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71984122, Length=304, Percent_Identity=24.6710526315789, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=631, Percent_Identity=30.9033280507132, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6323011, Length=272, Percent_Identity=27.2058823529412, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=304, Percent_Identity=25.9868421052632, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=33.2220367278798, Blast_Score=295, Evalue=8e-80,
Organism=Drosophila melanogaster, GI17136276, Length=299, Percent_Identity=28.4280936454849, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_ROSDO (Q16B16)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681513.1
- ProteinModelPortal:   Q16B16
- SMR:   Q16B16
- STRING:   Q16B16
- GeneID:   4197504
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1176
- NMPDR:   fig|375451.6.peg.1097
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   RDEN375451:RD1_1176-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66358; Mature: 66358

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMYRSHTCADLSAQDVGKTVRLSGWVHRVRDHGGVLFIDLRDHYGITQVLCDPDSPVFE
CCCCCCCCCCCCCHHHCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
QMEQVRAEWCIRVDGVVKARDASLINPKISTGEIELFVKDLEVLGASEELPLQVFGDQEY
HHHHHHHHHEEEECCEEEECCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCEEEECCCCC
PEETRLKYRYLDLRRANMQANMKLRSDVVASLRQQMWKADFREFQTPIITASSPEGARDF
CHHHCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHC
LVPSRLHPGRFYALPQAPQQFKQLLMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDIE
CCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQQDVFDVIQPVLTQVFEQFGNGKAVDQEWPQISYKDAALWYGSDKPDLRNPIKMQ
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHH
SVSEHFAGSGFAIFANLLEQEGTEIRAIPAPGGGSRKFCDRMNAFAQKEGLPGMGYIFWR
HHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
DQGAGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFFLGGKPKAFETVAGKARNHIGME
CCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHCCCCEE
LGLTDQNRFAFAWIVDFPIYEKDSETGRIDFEHNPFSMPQGGLEALQGDPLSVVGYQYDL
EECCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEEEEEC
SCNGYELVSGAIRNHKPEIMFKAFEIAGYGEDEVRKRFGGMVNAFQYGAPPHGGCAAGID
CCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
RIVMLLAEEANIREVILFPMNQRAEDLMMSAPSEPESEQLMELGLRVIPKD
HHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHCCCEECCCC
>Mature Secondary Structure
MHMYRSHTCADLSAQDVGKTVRLSGWVHRVRDHGGVLFIDLRDHYGITQVLCDPDSPVFE
CCCCCCCCCCCCCHHHCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
QMEQVRAEWCIRVDGVVKARDASLINPKISTGEIELFVKDLEVLGASEELPLQVFGDQEY
HHHHHHHHHEEEECCEEEECCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCEEEECCCCC
PEETRLKYRYLDLRRANMQANMKLRSDVVASLRQQMWKADFREFQTPIITASSPEGARDF
CHHHCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHC
LVPSRLHPGRFYALPQAPQQFKQLLMVSGFDKYFQIAPCFRDEDPRADRSPTDFYQLDIE
CCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQQDVFDVIQPVLTQVFEQFGNGKAVDQEWPQISYKDAALWYGSDKPDLRNPIKMQ
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHH
SVSEHFAGSGFAIFANLLEQEGTEIRAIPAPGGGSRKFCDRMNAFAQKEGLPGMGYIFWR
HHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
DQGAGMEAAGPLAKNIGPERTEAIRQQLGLGVGDAAFFLGGKPKAFETVAGKARNHIGME
CCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHCCCCEE
LGLTDQNRFAFAWIVDFPIYEKDSETGRIDFEHNPFSMPQGGLEALQGDPLSVVGYQYDL
EECCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEEEEEC
SCNGYELVSGAIRNHKPEIMFKAFEIAGYGEDEVRKRFGGMVNAFQYGAPPHGGCAAGID
CCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
RIVMLLAEEANIREVILFPMNQRAEDLMMSAPSEPESEQLMELGLRVIPKD
HHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA