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The map label for this gene is mclA [H]

Identifier: 110678490

GI number: 110678490

Start: 1120047

End: 1121024

Strand: Reverse

Name: mclA [H]

Synonym: RD1_1160

Alternate gene names: 110678490

Gene position: 1121024-1120047 (Counterclockwise)

Preceding gene: 110678498

Following gene: 110678489

Centisome position: 27.12

GC content: 58.38

Gene sequence:

>978_bases
ATGTCATTCCATCCTGTTGAACAAGCTCCCGGTCGCCTGAACAGATCGGAGCTGGCTGTTCCTGGTTCTCAGCCTCAAAT
GTTTGAAAAAGCGGCGCAATCCGACGTTGATGTGATCTTTCTGGACCTTGAGGACGCGGTCGCTCCGGATGAAAAGGAAC
AAGCGCGCAAGAACATCATTAAGGCCCTGAACGAGATCGACTGGGGCACCAAATCCATGTCGATTCGGATTAACGGTCTG
GACACACATTACATGTATCGTGATGTCGTGGACGTGGTGGAGCAAGCCGGAGAGCGGCTTGACCTCATCATGATTCCCAA
GGTCGGGACGGCTGCGGACGTCTATGCCGTGGACATGATGGTCACACAGATCGAGGACGCGAAAGGGTATTCAAAACGCA
TCGGGTTTGAACATATTATCGAGACCGCTCTGGGGATGCAGAACGTCTCTGAGATTGCCGCCGCTTCAAAGCGCAACGAA
AGCCTGCATTTCGGTGTGGCCGATTATGCGGCCTCCACGCGCGCGCGCACCACGATCATTGGCGGCGTAAATCCGGACTA
CTCCGTGCTGACCGACCCGGACAGCGATGGCAATCGCGCAACCCATTGGGGTGACATGTGGCACTATGCGCTCGCGCGGA
TGGTTGTCGCTGCACGTGCGAACGGGTTGCGTCCCATCGATGGCCCATTCGGTGATTTTCAGGATCCCGATGGGTATAAG
GCGGCCGCCTACCGCGCGGCGGTCCTGGGATGCGAGGGCAAATGGGCGATCCACCCCAGCCAGATCGCGCTGGCGAACGA
GGTGATGAGCCCGTCCGAGGCTGAAATCACCAAGGCCAAGCGCATTCTGGAAGCCATGGCGCAGGCCGAGGCCGAGGGCA
AAGGCGCCGTTTCCCTTGACGGCCGGCTCATCGACTACGCCTCCATCCGACAGGCAGAAGTCCTCGTCGAAAAGGCGCGG
CAAATTTCAGGTGCCTGA

Upstream 100 bases:

>100_bases
GATGCAGTATACCGAAATAGACTGAAACTCTCCTTGACATATTCAGTCCCGGAGAAGCTATCTGGCGCAATTTTATTACA
ATCCAGAAAGGGTACGATCC

Downstream 100 bases:

>100_bases
CCAACATTTCCGGCCTCTATCGATAGAGTAAAAACTAAACCGCGTTGTGCAGTTCTGAAGAACCTGCGCAACGCCACGAC
ACATTCCGTCACCTTGCCGT

Product: malyl-CoA lyase

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSFHPVEQAPGRLNRSELAVPGSQPQMFEKAAQSDVDVIFLDLEDAVAPDEKEQARKNIIKALNEIDWGTKSMSIRINGL
DTHYMYRDVVDVVEQAGERLDLIMIPKVGTAADVYAVDMMVTQIEDAKGYSKRIGFEHIIETALGMQNVSEIAAASKRNE
SLHFGVADYAASTRARTTIIGGVNPDYSVLTDPDSDGNRATHWGDMWHYALARMVVAARANGLRPIDGPFGDFQDPDGYK
AAAYRAAVLGCEGKWAIHPSQIALANEVMSPSEAEITKAKRILEAMAQAEAEGKGAVSLDGRLIDYASIRQAEVLVEKAR
QISGA

Sequences:

>Translated_325_residues
MSFHPVEQAPGRLNRSELAVPGSQPQMFEKAAQSDVDVIFLDLEDAVAPDEKEQARKNIIKALNEIDWGTKSMSIRINGL
DTHYMYRDVVDVVEQAGERLDLIMIPKVGTAADVYAVDMMVTQIEDAKGYSKRIGFEHIIETALGMQNVSEIAAASKRNE
SLHFGVADYAASTRARTTIIGGVNPDYSVLTDPDSDGNRATHWGDMWHYALARMVVAARANGLRPIDGPFGDFQDPDGYK
AAAYRAAVLGCEGKWAIHPSQIALANEVMSPSEAEITKAKRILEAMAQAEAEGKGAVSLDGRLIDYASIRQAEVLVEKAR
QISGA
>Mature_324_residues
SFHPVEQAPGRLNRSELAVPGSQPQMFEKAAQSDVDVIFLDLEDAVAPDEKEQARKNIIKALNEIDWGTKSMSIRINGLD
THYMYRDVVDVVEQAGERLDLIMIPKVGTAADVYAVDMMVTQIEDAKGYSKRIGFEHIIETALGMQNVSEIAAASKRNES
LHFGVADYAASTRARTTIIGGVNPDYSVLTDPDSDGNRATHWGDMWHYALARMVVAARANGLRPIDGPFGDFQDPDGYKA
AAYRAAVLGCEGKWAIHPSQIALANEVMSPSEAEITKAKRILEAMAQAEAEGKGAVSLDGRLIDYASIRQAEVLVEKARQ
ISGA

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=317, Percent_Identity=28.0757097791798, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI87081764, Length=320, Percent_Identity=29.6875, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 35406; Mature: 35275

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFHPVEQAPGRLNRSELAVPGSQPQMFEKAAQSDVDVIFLDLEDAVAPDEKEQARKNII
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH
KALNEIDWGTKSMSIRINGLDTHYMYRDVVDVVEQAGERLDLIMIPKVGTAADVYAVDMM
HHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
VTQIEDAKGYSKRIGFEHIIETALGMQNVSEIAAASKRNESLHFGVADYAASTRARTTII
HHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCCCEEEE
GGVNPDYSVLTDPDSDGNRATHWGDMWHYALARMVVAARANGLRPIDGPFGDFQDPDGYK
ECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
AAAYRAAVLGCEGKWAIHPSQIALANEVMSPSEAEITKAKRILEAMAQAEAEGKGAVSLD
HHHHHHHEECCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEC
GRLIDYASIRQAEVLVEKARQISGA
CEEECHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFHPVEQAPGRLNRSELAVPGSQPQMFEKAAQSDVDVIFLDLEDAVAPDEKEQARKNII
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH
KALNEIDWGTKSMSIRINGLDTHYMYRDVVDVVEQAGERLDLIMIPKVGTAADVYAVDMM
HHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
VTQIEDAKGYSKRIGFEHIIETALGMQNVSEIAAASKRNESLHFGVADYAASTRARTTII
HHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCCCEEEE
GGVNPDYSVLTDPDSDGNRATHWGDMWHYALARMVVAARANGLRPIDGPFGDFQDPDGYK
ECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
AAAYRAAVLGCEGKWAIHPSQIALANEVMSPSEAEITKAKRILEAMAQAEAEGKGAVSLD
HHHHHHHEECCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEC
GRLIDYASIRQAEVLVEKARQISGA
CEEECHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA