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The map label for this gene is mltB [H]

Identifier: 110678455

GI number: 110678455

Start: 1073413

End: 1074546

Strand: Reverse

Name: mltB [H]

Synonym: RD1_1120

Alternate gene names: 110678455

Gene position: 1074546-1073413 (Counterclockwise)

Preceding gene: 110678458

Following gene: 110678452

Centisome position: 26.0

GC content: 62.96

Gene sequence:

>1134_bases
ATGTATGTGACGCGGCGTAACTTCAACCTTGGCGCGCTGGCCCTCGCCTTGTCAGGCTGCACGGCCAGCCCAACCGGGCG
CCCCGCGACCACCGCCCTCCCCGATGATCTCAGGCCCGTTCGCAACAGTGCCTACGATGCCTGGGTGGCCAGCTTTCAGC
AACGTGCGCGCGCCGCGGGCATCACGGATGACACCCTGCGCCGCGCTTTCCAGGGCACGGGCTATTTGCCCGGTGTGGTG
CGCCGCGACCGCAACCAGACCGAATTCAAACGCTCGCTTGAAGACTATCTGTCCATCGCCGTGTCAGACGAACGCGTCGC
GCAGGGGCGCGATGCGCGGCGTCAATACGCCAGCGTTCTGGAAACGCTTGAGGCGCGCTACGGGGTGGATGCAAACATCA
TTACGGCCATCTGGGGGCTGGAAAGTTTTTACGGCGCGCGGCGCGGGAATGTGCCCGTGGTTTCCGCAACATCTACACTG
GCCTTCGATGGTCGGCGGGGGGCGTTTTTCGAAAAGCAGCTCATCGAAGCCCTGCGCATTGTGCAAAGCGGCGACATCAC
GGCGGACCGTCTTGTTGGCAGTTGGGCGGGCGCGATGGGTCACACCCAGTTCATCCCGACTTCGTATCAGGCTTTTGCCG
TGGATTTCACCGGCGACGGTCGCCGCGATATCTGGTCGGATGATCCGAGCGATGCGCTCGCATCTGCCGCAGCCTACCTT
GAGCGCAACGGATGGGTGCGCGGTCAACGATGGGGCAACGAGGTGACGGGCGCGGCCTCCGGCACGATCATTCAACCACA
GGAAAACGGCCCCCGGTTTGCAGTGAACCAAAATTTCCGCGTCATAAAACGCTACAACAATTCGGATGCCTATGCCATCG
GCGTCGGGCACCTGTCAGATCGCATCGCGGGGCGCGGTCCGCTGGTTACACCCTTTCCGCCGGACGAAAACGGCCTGACA
AAAGATGACCGGATCGCCCTGCAAATACGGCTGACCGCAAGCGGGTTTGACACCCAGGGGGCCGATGGTGTGATTGGTCC
CAATTCGCAAGCGGCCATTCGCGCCTATCAGGCCAGCCAGGGACTGCCGGTGACGGGCGTCCCTTCCCCGCAGCTTCTGG
ATCGTCTGCAGTAG

Upstream 100 bases:

>100_bases
GCTCAGGGCGCTCTGTCTGACGGCTGGATTTCGCTTTAGGTCCGCTCACTAAGCGCTTAAAGCTGGTGCCATCTTGGGCG
GACTTGCGTAGGAGAATGCG

Downstream 100 bases:

>100_bases
TCAGGCGCGGGCCAGCCGTGTTTCTTTCCACAGCGCATATGAATAAAGCGCCAAGGCACACCAGATCATCGGGAAGGCGA
TCATCCGCGCGCGGCCAAAT

Product: peptidoglycan binding domain-containing protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MYVTRRNFNLGALALALSGCTASPTGRPATTALPDDLRPVRNSAYDAWVASFQQRARAAGITDDTLRRAFQGTGYLPGVV
RRDRNQTEFKRSLEDYLSIAVSDERVAQGRDARRQYASVLETLEARYGVDANIITAIWGLESFYGARRGNVPVVSATSTL
AFDGRRGAFFEKQLIEALRIVQSGDITADRLVGSWAGAMGHTQFIPTSYQAFAVDFTGDGRRDIWSDDPSDALASAAAYL
ERNGWVRGQRWGNEVTGAASGTIIQPQENGPRFAVNQNFRVIKRYNNSDAYAIGVGHLSDRIAGRGPLVTPFPPDENGLT
KDDRIALQIRLTASGFDTQGADGVIGPNSQAAIRAYQASQGLPVTGVPSPQLLDRLQ

Sequences:

>Translated_377_residues
MYVTRRNFNLGALALALSGCTASPTGRPATTALPDDLRPVRNSAYDAWVASFQQRARAAGITDDTLRRAFQGTGYLPGVV
RRDRNQTEFKRSLEDYLSIAVSDERVAQGRDARRQYASVLETLEARYGVDANIITAIWGLESFYGARRGNVPVVSATSTL
AFDGRRGAFFEKQLIEALRIVQSGDITADRLVGSWAGAMGHTQFIPTSYQAFAVDFTGDGRRDIWSDDPSDALASAAAYL
ERNGWVRGQRWGNEVTGAASGTIIQPQENGPRFAVNQNFRVIKRYNNSDAYAIGVGHLSDRIAGRGPLVTPFPPDENGLT
KDDRIALQIRLTASGFDTQGADGVIGPNSQAAIRAYQASQGLPVTGVPSPQLLDRLQ
>Mature_377_residues
MYVTRRNFNLGALALALSGCTASPTGRPATTALPDDLRPVRNSAYDAWVASFQQRARAAGITDDTLRRAFQGTGYLPGVV
RRDRNQTEFKRSLEDYLSIAVSDERVAQGRDARRQYASVLETLEARYGVDANIITAIWGLESFYGARRGNVPVVSATSTL
AFDGRRGAFFEKQLIEALRIVQSGDITADRLVGSWAGAMGHTQFIPTSYQAFAVDFTGDGRRDIWSDDPSDALASAAAYL
ERNGWVRGQRWGNEVTGAASGTIIQPQENGPRFAVNQNFRVIKRYNNSDAYAIGVGHLSDRIAGRGPLVTPFPPDENGLT
KDDRIALQIRLTASGFDTQGADGVIGPNSQAAIRAYQASQGLPVTGVPSPQLLDRLQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=240, Percent_Identity=30.4166666666667, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40933; Mature: 40933

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVTRRNFNLGALALALSGCTASPTGRPATTALPDDLRPVRNSAYDAWVASFQQRARAAG
CEEEECCCCHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ITDDTLRRAFQGTGYLPGVVRRDRNQTEFKRSLEDYLSIAVSDERVAQGRDARRQYASVL
CCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHH
ETLEARYGVDANIITAIWGLESFYGARRGNVPVVSATSTLAFDGRRGAFFEKQLIEALRI
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHH
VQSGDITADRLVGSWAGAMGHTQFIPTSYQAFAVDFTGDGRRDIWSDDPSDALASAAAYL
HHCCCCCHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
ERNGWVRGQRWGNEVTGAASGTIIQPQENGPRFAVNQNFRVIKRYNNSDAYAIGVGHLSD
HHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEECCCEEEEEEECCCCEEEEEECCHHH
RIAGRGPLVTPFPPDENGLTKDDRIALQIRLTASGFDTQGADGVIGPNSQAAIRAYQASQ
HHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHEEEHHCC
GLPVTGVPSPQLLDRLQ
CCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MYVTRRNFNLGALALALSGCTASPTGRPATTALPDDLRPVRNSAYDAWVASFQQRARAAG
CEEEECCCCHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
ITDDTLRRAFQGTGYLPGVVRRDRNQTEFKRSLEDYLSIAVSDERVAQGRDARRQYASVL
CCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHH
ETLEARYGVDANIITAIWGLESFYGARRGNVPVVSATSTLAFDGRRGAFFEKQLIEALRI
HHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHH
VQSGDITADRLVGSWAGAMGHTQFIPTSYQAFAVDFTGDGRRDIWSDDPSDALASAAAYL
HHCCCCCHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
ERNGWVRGQRWGNEVTGAASGTIIQPQENGPRFAVNQNFRVIKRYNNSDAYAIGVGHLSD
HHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEECCCEEEEEEECCCCEEEEEECCHHH
RIAGRGPLVTPFPPDENGLTKDDRIALQIRLTASGFDTQGADGVIGPNSQAAIRAYQASQ
HHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHEEEHHCC
GLPVTGVPSPQLLDRLQ
CCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]