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The map label for this gene is phnD [H]

Identifier: 110678398

GI number: 110678398

Start: 1018152

End: 1019126

Strand: Reverse

Name: phnD [H]

Synonym: RD1_1057

Alternate gene names: 110678398

Gene position: 1019126-1018152 (Counterclockwise)

Preceding gene: 110678410

Following gene: 110678397

Centisome position: 24.66

GC content: 54.26

Gene sequence:

>975_bases
ATGAAATTCAATCTGAAACTGCTGCTTGGCGCCTCTGCTATCTGCATGCTTTCGCTGTCCACAAGCGCTTTTGCACAGGA
CTGTCCGCGCGGTGATCTTGATTCGCGCTTCTGCGATGTTGATGGGGATCTGATCGCCGATACACCAACCGACCCTGCCG
AGCAGATCGACCCGGACACGCTGATCTTTGCCTATACACCTGTCGAGGACCCAGCCGTTTACAAAGAGGCGTGGTCCGAT
TTCCTGACCCATCTGGAGTCCGAGACCGGCAAGGATGTCGTCTTCTTCCCCGTGCAGAACAACGCCGCCCAAATCGAGGC
GATGCGCTCTGGCCGTCTGCACATCGCCGGGTTCAACACAGGGTCGAACCCGCTGGCTGTGAATTGTGCGGGCTTCAGCC
CCTTTACGATCATGGCGTCCAAAGACGGCAATTTCGGGTATGAGATGGAAATCATCACCTATCCCGGCTCCGGAATCGAA
ACGGTTGAAGACATCAGAGGCAAATCCCTTGCCTTTACATCACCCACGTCGAATTCAGGATTCAAGGCACCCTCTGCGAT
CCTCAAGGGCGATTTCGACATGCTGCCCGAACGTGATTTCGAACCTGTGTTCTCCGGCAAGCACGACAACTCCATCCTCG
GTGTGGCCAATAAGGACTACGTCGCCGCCTCCATCGCCAACTCGGTCAAGTCGCGCATGATCAGCCGCGACGTCATCTCC
GAAGACGATGTGAAGGTCGTATATAAATCCCAAACCTTCCCGACCACAGGTTTCGGTACGGTCTATAACCTCAAGCCGGA
ACTGAAAGAGAAGATCCGCAACGCATTCTTCAATTTCGAATGGGAAGGCACCACGCTGCAGGCGGAATTCGAAAAATCGA
ACGAAGGCCAGTTTCTTGAGATGACCTACAAGGAATTCTGGGACGTGATCCGCAAGATTGATGCCGCCAATGGCGTCAGC
TATTCCTGCGAATAA

Upstream 100 bases:

>100_bases
TCTGACTCGCGCGATTAAAACAAGCCAGCTTTGATATTCACGTTGCAGAACGGCCATCACCGAACGCATAATCGCGCAAA
GAAAACCAAGGGAGAGAAAA

Downstream 100 bases:

>100_bases
ATCCTGATCGGGCGGATCTTTTTCAGGATCCGCCCTGTCCAATCATAAAATCGGGGGGGGCATGGTGATGCTGCGGCTTG
AGAAGCTCGTGAAGACATAT

Product: phosphonate ABC transporter, periplasmic binding protein, putative

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKFNLKLLLGASAICMLSLSTSAFAQDCPRGDLDSRFCDVDGDLIADTPTDPAEQIDPDTLIFAYTPVEDPAVYKEAWSD
FLTHLESETGKDVVFFPVQNNAAQIEAMRSGRLHIAGFNTGSNPLAVNCAGFSPFTIMASKDGNFGYEMEIITYPGSGIE
TVEDIRGKSLAFTSPTSNSGFKAPSAILKGDFDMLPERDFEPVFSGKHDNSILGVANKDYVAASIANSVKSRMISRDVIS
EDDVKVVYKSQTFPTTGFGTVYNLKPELKEKIRNAFFNFEWEGTTLQAEFEKSNEGQFLEMTYKEFWDVIRKIDAANGVS
YSCE

Sequences:

>Translated_324_residues
MKFNLKLLLGASAICMLSLSTSAFAQDCPRGDLDSRFCDVDGDLIADTPTDPAEQIDPDTLIFAYTPVEDPAVYKEAWSD
FLTHLESETGKDVVFFPVQNNAAQIEAMRSGRLHIAGFNTGSNPLAVNCAGFSPFTIMASKDGNFGYEMEIITYPGSGIE
TVEDIRGKSLAFTSPTSNSGFKAPSAILKGDFDMLPERDFEPVFSGKHDNSILGVANKDYVAASIANSVKSRMISRDVIS
EDDVKVVYKSQTFPTTGFGTVYNLKPELKEKIRNAFFNFEWEGTTLQAEFEKSNEGQFLEMTYKEFWDVIRKIDAANGVS
YSCE
>Mature_324_residues
MKFNLKLLLGASAICMLSLSTSAFAQDCPRGDLDSRFCDVDGDLIADTPTDPAEQIDPDTLIFAYTPVEDPAVYKEAWSD
FLTHLESETGKDVVFFPVQNNAAQIEAMRSGRLHIAGFNTGSNPLAVNCAGFSPFTIMASKDGNFGYEMEIITYPGSGIE
TVEDIRGKSLAFTSPTSNSGFKAPSAILKGDFDMLPERDFEPVFSGKHDNSILGVANKDYVAASIANSVKSRMISRDVIS
EDDVKVVYKSQTFPTTGFGTVYNLKPELKEKIRNAFFNFEWEGTTLQAEFEKSNEGQFLEMTYKEFWDVIRKIDAANGVS
YSCE

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=238, Percent_Identity=27.7310924369748, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 35719; Mature: 35719

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNLKLLLGASAICMLSLSTSAFAQDCPRGDLDSRFCDVDGDLIADTPTDPAEQIDPDT
CEEEEEEEECCCEEEEEEECCHHHHHHCCCCCCCCEEECCCCCEEECCCCCHHHHCCCCE
LIFAYTPVEDPAVYKEAWSDFLTHLESETGKDVVFFPVQNNAAQIEAMRSGRLHIAGFNT
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCEEEEEEECC
GSNPLAVNCAGFSPFTIMASKDGNFGYEMEIITYPGSGIETVEDIRGKSLAFTSPTSNSG
CCCCEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHCCCCEEEEECCCCCCC
FKAPSAILKGDFDMLPERDFEPVFSGKHDNSILGVANKDYVAASIANSVKSRMISRDVIS
CCCCHHHHCCCHHCCCCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
EDDVKVVYKSQTFPTTGFGTVYNLKPELKEKIRNAFFNFEWEGTTLQAEFEKSNEGQFLE
CCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEE
MTYKEFWDVIRKIDAANGVSYSCE
EHHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MKFNLKLLLGASAICMLSLSTSAFAQDCPRGDLDSRFCDVDGDLIADTPTDPAEQIDPDT
CEEEEEEEECCCEEEEEEECCHHHHHHCCCCCCCCEEECCCCCEEECCCCCHHHHCCCCE
LIFAYTPVEDPAVYKEAWSDFLTHLESETGKDVVFFPVQNNAAQIEAMRSGRLHIAGFNT
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCEEEEEEECC
GSNPLAVNCAGFSPFTIMASKDGNFGYEMEIITYPGSGIETVEDIRGKSLAFTSPTSNSG
CCCCEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHCCCCEEEEECCCCCCC
FKAPSAILKGDFDMLPERDFEPVFSGKHDNSILGVANKDYVAASIANSVKSRMISRDVIS
CCCCHHHHCCCHHCCCCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCC
EDDVKVVYKSQTFPTTGFGTVYNLKPELKEKIRNAFFNFEWEGTTLQAEFEKSNEGQFLE
CCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCEEE
MTYKEFWDVIRKIDAANGVSYSCE
EHHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]