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The map label for this gene is pheT [H]

Identifier: 110678357

GI number: 110678357

Start: 974748

End: 977147

Strand: Direct

Name: pheT [H]

Synonym: RD1_1013

Alternate gene names: 110678357

Gene position: 974748-977147 (Clockwise)

Preceding gene: 110678356

Following gene: 110678358

Centisome position: 23.58

GC content: 61.71

Gene sequence:

>2400_bases
ATGAAATTCACACTCTCTTGGCTCAAAGAGCATCTTGATACCTCTGCCACGCTGGACGAGATCACCTATGCGCTGACCGA
CCTTGGTCTTGAGGTCGAAGGGGTCGAGGACCGTGCGGGCAAGCTGCGGGACTTTACTATCGGTTACGTCAAATCAGCGG
AAAAACACCCGGATGCGGACCGCCTCAAGGTCTGTCAGGTAGCGACTGATGAGGGCGACAAGCAGATCATTTGCGGCGCC
CCGAATGCGCGCGAAGGCATCACCGTCGTCGTGGCGAAACCCGGCGTCTATGTGCCCGGTATTGATACCACCATCGGCGT
GGGCAAAATTCGCGGGATCGAAAGCTTTGGCATGATGGCCTCCGAGCGCGAGATGGAGCTGTCCGAAGAACACGACGGCA
TCATCGAATTGCCGAGCGGAGAGGTCGGGCGGTCTTTCGTCGACTGGCTGGCAGAGCATGACCCGGCCAAGGTTGACCCG
GTGATCGAGATCGCGATCACCCCGAACCGGCCCGATGCGCTCGGTGTGCGCGGGATCGCGCGTGATCTGGCGGCGCGCGG
CCTTGGCACGTTGAAGCCGCGCGATGTTGCCGCCGTCGAAGGGGTCTTCCCCTGTCCGGTGAGCATCACCATCGAGGCGG
ATACGCTGGAGCAGTGTCCGGTTTTCTTTGGCCGGGTGATCCGGGGTGTCAAGAACGGCCCAAGCCCGCAGTGGCTGCAG
GACAAACTACGCGCCATCGGTCTGCGCCCGATTTCGTTCCTCGTCGATGTGACGAACTTCTTCACCTATGATCGCAACCG
CCCCCTGCACGTCTTTGACGCCGACAAGATCGAAGGCAAAACCCTGCGGGTCCATCGCGCCAAGGGTGGCGAAACCCTCG
TGGGGCTGGACGAAAAGGAATATACCTTTGCAGAAGGCATGACGCTGATCTCTGACGCGGCCAATGTGGAAAGCATCGCG
GGGGTCATGGGCGGCCTTGCGACGGGCTGCACCGCAGAGACCACGAATGTCTTTATCGAAGCGGCCTATTTCGATCCGGT
CCGCACGGCCTATACCGGTCGCGCGCTGAAGATCAATTCCGACGCGCGGTATCGGTTTGAACGGGGCATCGACCCGGAAT
GGACGCCCTACGGGATCGAACATGCAACGCATATGATCCTTGAGCATGCAGGCGGTGAGCCCTCCGAGGTCATCGTGGCG
GGCAAGATACCTGACACCAACCGGGCCTATAAACTGGATGCCGCGCGGGTGCAGTCGCTGGTGGGCATGGACATCCCCGA
AAGCAGTCAGCGCCAGACGCTGACCGCGCTGGGGTTCCGCCTTGAAGGCAACATGGCCCATGTGCCAAGCTGGCGCCCGG
ATGTGCAGGGCGAAGCGGATCTGGTCGAAGAAGTGGCGCGGATTGCCTCGCTTACCAAGCTGAAAGGCAGACCGCTGCCC
CGGCTGGATGAAGGCGTGTCGCGCCCGATCCTGTCGCCGATCCAGCGGCGCGAAGCCATGGCACGGCGGACCTGCGCGGC
CTTGGGGTACAACGAATGTGTCAGCTACAGCTTTATTGATCAGGCCTCTGCCAAGCTGTTCGGTGGCGGAGAGGACGTCA
CCCGGCTGGAAAACCCGATCAGCAGCGACATGAGCCACATGCGTCCCGCATTGCTTCCCGGTCTCTTGCAGGCGGCAGCG
CGCAATCAGGCGCGGGGCAATATGAACCTCGCGCTGTTTGAGGTCGGCCCTGCCTTTCACGGCGGCGAACCCGGAGAGCA
GCATTTGCTGGTCAGTGGGGTGCTGATCGGGCGGACGGAACCCAAGGATGTGCATGGTGCGTCCCGCGCTGTTGACGTCT
ATGATGTCAAAGCGGATGCGGAAGCGGTTCTGGCGGCCATCGGTGCACCTGCCAAGGTACAGATTTTGCGCGGTGCCGCC
CCGTGGTGGCATCCCGGTCGGCATGGGATGATCTGTCTGGGGCCCAAGAAAGTTCTGGGTGTATTCGGCGAAATCCACCC
GCGTGTGTTGGCGGCGATGGACGTCAAAGGGCCCGCGATGGGCTTTACCATCTGGCCGGCCGAGGTGCCGCTGCCCCGCA
AGAGTGGCGCGACCCGTGCCGCATTGCAGATTAGCGACCTGCAGGCGGTGGAGCGCGATTTCGCCTTTGTTGTCGATAGC
GGTGTGGAGGCACTGACCCTCGTCAACGCGGCAATGGGGGCTGATAAGGCGCTGATCGAGGATGTGCGCGTCTTTGATGA
ATTTATCGGCGGCTCTTTGGGGGAAGGAAAGAAATCCCTTGCGCTGACAGTGCGGATGCAACCCGCGACCCAGACGCTGA
CCGATAAAGACATCGAAGCGGTCAGCGCAAAGGTCGTGGAAAAGGTGATCAAGGCCACGGGTGGAACGCTGCGCGCCTGA

Upstream 100 bases:

>100_bases
CCGAGGGACATTTCGGCGTAAAACTAGACTTTAATTTTGACTGATTTGTAGTCTGTTACTGCCCGAAGCTTTTGGTTTGA
ACACAGCATAGGACCTTAGA

Downstream 100 bases:

>100_bases
TACGTCCGCGCCGGTTGGGCGCTGCAGCATGTAATTGAATTCAAAGGAGATGCAGCATGACATTGAAAGTGTATCTGTCG
GGTGAAATCCACACTGACTG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 799; Mature: 799

Protein sequence:

>799_residues
MKFTLSWLKEHLDTSATLDEITYALTDLGLEVEGVEDRAGKLRDFTIGYVKSAEKHPDADRLKVCQVATDEGDKQIICGA
PNAREGITVVVAKPGVYVPGIDTTIGVGKIRGIESFGMMASEREMELSEEHDGIIELPSGEVGRSFVDWLAEHDPAKVDP
VIEIAITPNRPDALGVRGIARDLAARGLGTLKPRDVAAVEGVFPCPVSITIEADTLEQCPVFFGRVIRGVKNGPSPQWLQ
DKLRAIGLRPISFLVDVTNFFTYDRNRPLHVFDADKIEGKTLRVHRAKGGETLVGLDEKEYTFAEGMTLISDAANVESIA
GVMGGLATGCTAETTNVFIEAAYFDPVRTAYTGRALKINSDARYRFERGIDPEWTPYGIEHATHMILEHAGGEPSEVIVA
GKIPDTNRAYKLDAARVQSLVGMDIPESSQRQTLTALGFRLEGNMAHVPSWRPDVQGEADLVEEVARIASLTKLKGRPLP
RLDEGVSRPILSPIQRREAMARRTCAALGYNECVSYSFIDQASAKLFGGGEDVTRLENPISSDMSHMRPALLPGLLQAAA
RNQARGNMNLALFEVGPAFHGGEPGEQHLLVSGVLIGRTEPKDVHGASRAVDVYDVKADAEAVLAAIGAPAKVQILRGAA
PWWHPGRHGMICLGPKKVLGVFGEIHPRVLAAMDVKGPAMGFTIWPAEVPLPRKSGATRAALQISDLQAVERDFAFVVDS
GVEALTLVNAAMGADKALIEDVRVFDEFIGGSLGEGKKSLALTVRMQPATQTLTDKDIEAVSAKVVEKVIKATGGTLRA

Sequences:

>Translated_799_residues
MKFTLSWLKEHLDTSATLDEITYALTDLGLEVEGVEDRAGKLRDFTIGYVKSAEKHPDADRLKVCQVATDEGDKQIICGA
PNAREGITVVVAKPGVYVPGIDTTIGVGKIRGIESFGMMASEREMELSEEHDGIIELPSGEVGRSFVDWLAEHDPAKVDP
VIEIAITPNRPDALGVRGIARDLAARGLGTLKPRDVAAVEGVFPCPVSITIEADTLEQCPVFFGRVIRGVKNGPSPQWLQ
DKLRAIGLRPISFLVDVTNFFTYDRNRPLHVFDADKIEGKTLRVHRAKGGETLVGLDEKEYTFAEGMTLISDAANVESIA
GVMGGLATGCTAETTNVFIEAAYFDPVRTAYTGRALKINSDARYRFERGIDPEWTPYGIEHATHMILEHAGGEPSEVIVA
GKIPDTNRAYKLDAARVQSLVGMDIPESSQRQTLTALGFRLEGNMAHVPSWRPDVQGEADLVEEVARIASLTKLKGRPLP
RLDEGVSRPILSPIQRREAMARRTCAALGYNECVSYSFIDQASAKLFGGGEDVTRLENPISSDMSHMRPALLPGLLQAAA
RNQARGNMNLALFEVGPAFHGGEPGEQHLLVSGVLIGRTEPKDVHGASRAVDVYDVKADAEAVLAAIGAPAKVQILRGAA
PWWHPGRHGMICLGPKKVLGVFGEIHPRVLAAMDVKGPAMGFTIWPAEVPLPRKSGATRAALQISDLQAVERDFAFVVDS
GVEALTLVNAAMGADKALIEDVRVFDEFIGGSLGEGKKSLALTVRMQPATQTLTDKDIEAVSAKVVEKVIKATGGTLRA
>Mature_799_residues
MKFTLSWLKEHLDTSATLDEITYALTDLGLEVEGVEDRAGKLRDFTIGYVKSAEKHPDADRLKVCQVATDEGDKQIICGA
PNAREGITVVVAKPGVYVPGIDTTIGVGKIRGIESFGMMASEREMELSEEHDGIIELPSGEVGRSFVDWLAEHDPAKVDP
VIEIAITPNRPDALGVRGIARDLAARGLGTLKPRDVAAVEGVFPCPVSITIEADTLEQCPVFFGRVIRGVKNGPSPQWLQ
DKLRAIGLRPISFLVDVTNFFTYDRNRPLHVFDADKIEGKTLRVHRAKGGETLVGLDEKEYTFAEGMTLISDAANVESIA
GVMGGLATGCTAETTNVFIEAAYFDPVRTAYTGRALKINSDARYRFERGIDPEWTPYGIEHATHMILEHAGGEPSEVIVA
GKIPDTNRAYKLDAARVQSLVGMDIPESSQRQTLTALGFRLEGNMAHVPSWRPDVQGEADLVEEVARIASLTKLKGRPLP
RLDEGVSRPILSPIQRREAMARRTCAALGYNECVSYSFIDQASAKLFGGGEDVTRLENPISSDMSHMRPALLPGLLQAAA
RNQARGNMNLALFEVGPAFHGGEPGEQHLLVSGVLIGRTEPKDVHGASRAVDVYDVKADAEAVLAAIGAPAKVQILRGAA
PWWHPGRHGMICLGPKKVLGVFGEIHPRVLAAMDVKGPAMGFTIWPAEVPLPRKSGATRAALQISDLQAVERDFAFVVDS
GVEALTLVNAAMGADKALIEDVRVFDEFIGGSLGEGKKSLALTVRMQPATQTLTDKDIEAVSAKVVEKVIKATGGTLRA

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=821, Percent_Identity=34.3483556638246, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86154; Mature: 86154

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50886 TRBD ; PS00141 ASP_PROTEASE ; PS00449 ATPASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLKEHLDTSATLDEITYALTDLGLEVEGVEDRAGKLRDFTIGYVKSAEKHPDAD
CCEEHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCHHCCCCEEEEEHHHHCCCCCCCHH
RLKVCQVATDEGDKQIICGAPNAREGITVVVAKPGVYVPGIDTTIGVGKIRGIESFGMMA
HEEEEEEECCCCCCEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCHHCCCHHHHCCCC
SEREMELSEEHDGIIELPSGEVGRSFVDWLAEHDPAKVDPVIEIAITPNRPDALGVRGIA
CCHHCHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHH
RDLAARGLGTLKPRDVAAVEGVFPCPVSITIEADTLEQCPVFFGRVIRGVKNGPSPQWLQ
HHHHHCCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHH
DKLRAIGLRPISFLVDVTNFFTYDRNRPLHVFDADKIEGKTLRVHRAKGGETLVGLDEKE
HHHHHCCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCC
YTFAEGMTLISDAANVESIAGVMGGLATGCTAETTNVFIEAAYFDPVRTAYTGRALKINS
CHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHCCCEEEECC
DARYRFERGIDPEWTPYGIEHATHMILEHAGGEPSEVIVAGKIPDTNRAYKLDAARVQSL
CCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECHHHHHHH
VGMDIPESSQRQTLTALGFRLEGNMAHVPSWRPDVQGEADLVEEVARIASLTKLKGRPLP
HCCCCCCCCHHHHHHHHCEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
RLDEGVSRPILSPIQRREAMARRTCAALGYNECVSYSFIDQASAKLFGGGEDVTRLENPI
CHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHCCHHCCCCCCHHHHHHCHH
SSDMSHMRPALLPGLLQAAARNQARGNMNLALFEVGPAFHGGEPGEQHLLVSGVLIGRTE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEEECCC
PKDVHGASRAVDVYDVKADAEAVLAAIGAPAKVQILRGAAPWWHPGRHGMICLGPKKVLG
CCCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHH
VFGEIHPRVLAAMDVKGPAMGFTIWPAEVPLPRKSGATRAALQISDLQAVERDFAFVVDS
HHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEECC
GVEALTLVNAAMGADKALIEDVRVFDEFIGGSLGEGKKSLALTVRMQPATQTLTDKDIEA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHCCHHHHHH
VSAKVVEKVIKATGGTLRA
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFTLSWLKEHLDTSATLDEITYALTDLGLEVEGVEDRAGKLRDFTIGYVKSAEKHPDAD
CCEEHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCHHCCCCEEEEEHHHHCCCCCCCHH
RLKVCQVATDEGDKQIICGAPNAREGITVVVAKPGVYVPGIDTTIGVGKIRGIESFGMMA
HEEEEEEECCCCCCEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCHHCCCHHHHCCCC
SEREMELSEEHDGIIELPSGEVGRSFVDWLAEHDPAKVDPVIEIAITPNRPDALGVRGIA
CCHHCHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHH
RDLAARGLGTLKPRDVAAVEGVFPCPVSITIEADTLEQCPVFFGRVIRGVKNGPSPQWLQ
HHHHHCCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHH
DKLRAIGLRPISFLVDVTNFFTYDRNRPLHVFDADKIEGKTLRVHRAKGGETLVGLDEKE
HHHHHCCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCC
YTFAEGMTLISDAANVESIAGVMGGLATGCTAETTNVFIEAAYFDPVRTAYTGRALKINS
CHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHCCCEEEECC
DARYRFERGIDPEWTPYGIEHATHMILEHAGGEPSEVIVAGKIPDTNRAYKLDAARVQSL
CCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECHHHHHHH
VGMDIPESSQRQTLTALGFRLEGNMAHVPSWRPDVQGEADLVEEVARIASLTKLKGRPLP
HCCCCCCCCHHHHHHHHCEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
RLDEGVSRPILSPIQRREAMARRTCAALGYNECVSYSFIDQASAKLFGGGEDVTRLENPI
CHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHCCHHCCCCCCHHHHHHCHH
SSDMSHMRPALLPGLLQAAARNQARGNMNLALFEVGPAFHGGEPGEQHLLVSGVLIGRTE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEEECCC
PKDVHGASRAVDVYDVKADAEAVLAAIGAPAKVQILRGAAPWWHPGRHGMICLGPKKVLG
CCCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEECHHHHHH
VFGEIHPRVLAAMDVKGPAMGFTIWPAEVPLPRKSGATRAALQISDLQAVERDFAFVVDS
HHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEECC
GVEALTLVNAAMGADKALIEDVRVFDEFIGGSLGEGKKSLALTVRMQPATQTLTDKDIEA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHCCHHHHHH
VSAKVVEKVIKATGGTLRA
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA