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The map label for this gene is pheS

Identifier: 110678356

GI number: 110678356

Start: 973220

End: 974293

Strand: Direct

Name: pheS

Synonym: RD1_1012

Alternate gene names: 110678356

Gene position: 973220-974293 (Clockwise)

Preceding gene: 110678355

Following gene: 110678357

Centisome position: 23.55

GC content: 62.29

Gene sequence:

>1074_bases
ATGGACGATCTCAAGCAAAAATACTTAAGCCAGATAGCGGCAGCGCAGGACGAGGCGGGGCTGGAAGCGATCCGCCTGGC
GGCTGTCGGCAAGAAGGGTGAGGTCGCCCTGAAAATGCGCGAGTTGGGCAAGATGACCCCGGAAGAACGACAGGTCGCTG
GCCCGGCCCTCAATGCCCTCAAAGATGAGATCAATTCGGCGCTGGCTGCCAAGAAAGCGGGCTTGGCGGATGCCGCGCTG
GATGCGCGTTTGCGCGACGAGTGGCTGGATGTGACCCTGCCCGCGCGCGGACGTCCGCGCGGGACGATCCACCCGGTCAG
TCAGGTCACCGAAGAAGTCACGGCCATCTTTGGCGAAATGGGGTTTTCGGTCGCCGAAGGGCCGCGCATTGATACCGATT
GGTATAACTTTGACGCGCTCAACATTCCGGGCCACCACCCGGCGCGGGCCGAGATGGACACGTTTTACATGACGCGCGCC
GAAGGCGACGACCGCCCGCCCCATGTGCTGCGCACCCATACGTCCCCTGTGCAAATCCGCACGATGGAGGCGCAGGGCGC
GCCCTTGCGCATCATCTGTCCCGGTGGCGTCTACCGCGCGGATTACGACCAGACGCATACGCCCATGTTCCATCAGGTCG
AAGGACTGGCGATCGACAAGGACATCTCCATGGCGAACCTGAAATGGGTGCTGGAGGAGTTCTTTGCCGCCTTCTTTGAA
ATCGACGGGATCAAGACGCGGTTCCGCGCGTCGCACTTCCCCTTCACCGAACCCTCGGCGGAGGTGGATATCCAGTGTTC
GTGGATTGATGGGCAATTGCGCATCGGCGAAGGGGACGACTGGCTCGAAGTTCTTGGCTCCGGCATGGTACATCCCAAGG
TGCTGGCCGCCGGCGGGATTGACCCCGACCAGTGGCAGGGCTTCGCCTTTGGCATGGGCATCGACCGCATCGCCATGCTG
AAATACGGCATCCCGGATTTGCGCGCGTTCTTTGATTCCGACCTGCGGTGGCTGCGGCATTACGGGTTTGCCTCGCTGGA
TCAGCCGACGCTGCATGGGGGGCTGAGTAGGTGA

Upstream 100 bases:

>100_bases
ATGTGCCTCAGCCGTCAACGCCTCAGCCCCGCACAACTGCTGGCTTGCCATCAGGGACTGTTCTGCTAGGAAACAGGGCA
AATCAAGGGACGCGCGGAAT

Downstream 100 bases:

>100_bases
TTGGTTGGTTCAAAAAGAAGAAAGCCGAAACTGTGAATTTGCCGTTGCTTGATTGTGGCGGGCAATACCAATTCGATATT
GTGGGTGAAGCATCTTACCA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MDDLKQKYLSQIAAAQDEAGLEAIRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNALKDEINSALAAKKAGLADAAL
DARLRDEWLDVTLPARGRPRGTIHPVSQVTEEVTAIFGEMGFSVAEGPRIDTDWYNFDALNIPGHHPARAEMDTFYMTRA
EGDDRPPHVLRTHTSPVQIRTMEAQGAPLRIICPGGVYRADYDQTHTPMFHQVEGLAIDKDISMANLKWVLEEFFAAFFE
IDGIKTRFRASHFPFTEPSAEVDIQCSWIDGQLRIGEGDDWLEVLGSGMVHPKVLAAGGIDPDQWQGFAFGMGIDRIAML
KYGIPDLRAFFDSDLRWLRHYGFASLDQPTLHGGLSR

Sequences:

>Translated_357_residues
MDDLKQKYLSQIAAAQDEAGLEAIRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNALKDEINSALAAKKAGLADAAL
DARLRDEWLDVTLPARGRPRGTIHPVSQVTEEVTAIFGEMGFSVAEGPRIDTDWYNFDALNIPGHHPARAEMDTFYMTRA
EGDDRPPHVLRTHTSPVQIRTMEAQGAPLRIICPGGVYRADYDQTHTPMFHQVEGLAIDKDISMANLKWVLEEFFAAFFE
IDGIKTRFRASHFPFTEPSAEVDIQCSWIDGQLRIGEGDDWLEVLGSGMVHPKVLAAGGIDPDQWQGFAFGMGIDRIAML
KYGIPDLRAFFDSDLRWLRHYGFASLDQPTLHGGLSR
>Mature_357_residues
MDDLKQKYLSQIAAAQDEAGLEAIRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNALKDEINSALAAKKAGLADAAL
DARLRDEWLDVTLPARGRPRGTIHPVSQVTEEVTAIFGEMGFSVAEGPRIDTDWYNFDALNIPGHHPARAEMDTFYMTRA
EGDDRPPHVLRTHTSPVQIRTMEAQGAPLRIICPGGVYRADYDQTHTPMFHQVEGLAIDKDISMANLKWVLEEFFAAFFE
IDGIKTRFRASHFPFTEPSAEVDIQCSWIDGQLRIGEGDDWLEVLGSGMVHPKVLAAGGIDPDQWQGFAFGMGIDRIAML
KYGIPDLRAFFDSDLRWLRHYGFASLDQPTLHGGLSR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=30.4832713754647, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI5729820, Length=299, Percent_Identity=29.0969899665552, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1788007, Length=330, Percent_Identity=53.030303030303, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32563657, Length=289, Percent_Identity=30.4498269896194, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=289, Percent_Identity=30.4498269896194, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32566635, Length=264, Percent_Identity=32.9545454545455, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=269, Percent_Identity=30.1115241635688, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=99, Percent_Identity=37.3737373737374, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858079, Length=277, Percent_Identity=29.9638989169675, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137424, Length=300, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ROSDO (Q16BG6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681363.1
- ProteinModelPortal:   Q16BG6
- SMR:   Q16BG6
- STRING:   Q16BG6
- GeneID:   4198258
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1012
- NMPDR:   fig|375451.6.peg.949
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q16BG6
- ProtClustDB:   PRK00488
- BioCyc:   RDEN375451:RD1_1012-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39586; Mature: 39586

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLKQKYLSQIAAAQDEAGLEAIRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNAL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHH
KDEINSALAAKKAGLADAALDARLRDEWLDVTLPARGRPRGTIHPVSQVTEEVTAIFGEM
HHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
GFSVAEGPRIDTDWYNFDALNIPGHHPARAEMDTFYMTRAEGDDRPPHVLRTHTSPVQIR
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHEEEEECCCCCCCCCEEEECCCCEEEE
TMEAQGAPLRIICPGGVYRADYDQTHTPMFHQVEGLAIDKDISMANLKWVLEEFFAAFFE
EEECCCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHH
IDGIKTRFRASHFPFTEPSAEVDIQCSWIDGQLRIGEGDDWLEVLGSGMVHPKVLAAGGI
HCCCHHHHHCCCCCCCCCCCCEEEEEEEECCEEEECCCCHHHHHHCCCCCCCEEEEECCC
DPDQWQGFAFGMGIDRIAMLKYGIPDLRAFFDSDLRWLRHYGFASLDQPTLHGGLSR
CHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDDLKQKYLSQIAAAQDEAGLEAIRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNAL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHH
KDEINSALAAKKAGLADAALDARLRDEWLDVTLPARGRPRGTIHPVSQVTEEVTAIFGEM
HHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
GFSVAEGPRIDTDWYNFDALNIPGHHPARAEMDTFYMTRAEGDDRPPHVLRTHTSPVQIR
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHEEEEECCCCCCCCCEEEECCCCEEEE
TMEAQGAPLRIICPGGVYRADYDQTHTPMFHQVEGLAIDKDISMANLKWVLEEFFAAFFE
EEECCCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHH
IDGIKTRFRASHFPFTEPSAEVDIQCSWIDGQLRIGEGDDWLEVLGSGMVHPKVLAAGGI
HCCCHHHHHCCCCCCCCCCCCEEEEEEEECCEEEECCCCHHHHHHCCCCCCCEEEEECCC
DPDQWQGFAFGMGIDRIAMLKYGIPDLRAFFDSDLRWLRHYGFASLDQPTLHGGLSR
CHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA