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The map label for this gene is pyk [H]

Identifier: 110678351

GI number: 110678351

Start: 968887

End: 970335

Strand: Direct

Name: pyk [H]

Synonym: RD1_1007

Alternate gene names: 110678351

Gene position: 968887-970335 (Clockwise)

Preceding gene: 110678349

Following gene: 110678353

Centisome position: 23.44

GC content: 61.08

Gene sequence:

>1449_bases
ATGAGACGCCATCGCAATGTAAAAATCGTTGCCACTCTGGGGCCTGCGTCCAGTGATTACGACATGATCCGGGCTTTGCA
TGACGCGGGTGCGGATGTGTTTCGCCTGAACATGAGCCACGGCACCCACGACGAACTGCGCGAGCGGCACCGGATTATCC
GCCAGATAGAAGAGGACACGGGCGGCAGCATCTGTATTCTGGCGGACCTGCAAGGGCCAAAGCTGCGCGTGGGTGTCTTT
GCCAATGAAGAGGGCGAAATGCTGGAGGAAGGCGCGGCTTTCCGCATGGATCTGGACGAAGCGCCCGGGGACAAGACGCG
TGTGAACCTGCCCCATCCGGAGATTTTCGAAGCGCTCGAACCCGGTGCCAGCCTGCTGGTCAATGATGGTAAAATTCGCC
TCAAGGTGGAGGATTGCAGCAAAACCCACGCCAATTGCACCGTGATTGCGGGCGGCGTGATTTCGAACCGCAAGGGAGTC
AATGTGCCTGATGTGGTGTTGCCCCTCGCCGCGCTTTCGGACAAGGACCGCAAGGACCTGGAGTTCGCCTGCGAGCTGGG
CGTTGACTGGCTTGCGCTGTCCTTCGTGCAGCGTGCGGATGATGTGCTGGAAGCCTCGGATCTGGCCAATGGCCGCGCGG
CGATCCTCAGCAAGATCGAAAAGCCCGCAGCGGTGCATAACTTCGATGAAATCCTTGAAGTCAGCGACGGGATCATGGTC
GCGCGCGGTGATCTGGGCGTGGAGTTGCCCGTGCAAAACGTGCCGCCCATTCAAAAGCGGCTGGTGCGCAAATGCCGCCG
TGCCGCCAAGCCTGTCATCGTCGCGACCCAGATGATGGAAAGCATGATCGAAAGCCCCGTGCCCACCCGCGCCGAGGTTT
CCGATGTGGCCACTGCAATCTATGAGGGTGCGGATGCAGTGATGTTGTCCGCAGAATCCGCCGCCGGTGCCTATCCGGTT
GAGGCGGTGACCACCATGAACAACGTCGCCATCGAGGTCGAACAGGACCCGACCTATACGCAGATCATCGAAGCCTCCCG
CGAGGCCAAGGGACGGTCCGTTGCAGACGGGATCGTGTCGGCGGCGCGCGAAATTGCCGAGACGACCAACATCAAGGCGA
TCTGCTGTTTCAGCCAAAGCGGGACAACGGCGATGCTGACCGCGCGCGAACGCCCGCGGGTGCCGATCATTGTCATGACC
TCCAAGGCGCGCACGGCCCGGCGGCTGACACTGGCCTGGGGGTGTAACTGCGTCATGACAGACCAGCTTGAGCGTTTCAA
ACAGGCGGTGGTCAATTCTGCGCGCGTGGCGACCCGTCAGGGCTATGCCACCAAAGAGGACCTGATCGTGGTCACGGCTG
GCGTACCCTTCAACGTGCCGGGCAGTACCAACATCCTGCGCGTGGCGCCTTGTGACGAGCGGCTGATCTTTAACACGGAT
CCGGAATAG

Upstream 100 bases:

>100_bases
CATGGTTGCCCTTGGGTGCGGCATGACCCATAAAGCCCAAAAGCCGTTAGCGATAACTCAAGCGGATTGATGAATTGAAA
AGAAGAGGGACCGGACCTTT

Downstream 100 bases:

>100_bases
GGCTTGGCAGACTGACCCAACACAGGCGTCTTTGCTAACCTGCGATGCCTTCGGCGGCCCGCGCGCTGGCGCAATACTCG
GAAGATACGTTGAGATTGCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MRRHRNVKIVATLGPASSDYDMIRALHDAGADVFRLNMSHGTHDELRERHRIIRQIEEDTGGSICILADLQGPKLRVGVF
ANEEGEMLEEGAAFRMDLDEAPGDKTRVNLPHPEIFEALEPGASLLVNDGKIRLKVEDCSKTHANCTVIAGGVISNRKGV
NVPDVVLPLAALSDKDRKDLEFACELGVDWLALSFVQRADDVLEASDLANGRAAILSKIEKPAAVHNFDEILEVSDGIMV
ARGDLGVELPVQNVPPIQKRLVRKCRRAAKPVIVATQMMESMIESPVPTRAEVSDVATAIYEGADAVMLSAESAAGAYPV
EAVTTMNNVAIEVEQDPTYTQIIEASREAKGRSVADGIVSAAREIAETTNIKAICCFSQSGTTAMLTARERPRVPIIVMT
SKARTARRLTLAWGCNCVMTDQLERFKQAVVNSARVATRQGYATKEDLIVVTAGVPFNVPGSTNILRVAPCDERLIFNTD
PE

Sequences:

>Translated_482_residues
MRRHRNVKIVATLGPASSDYDMIRALHDAGADVFRLNMSHGTHDELRERHRIIRQIEEDTGGSICILADLQGPKLRVGVF
ANEEGEMLEEGAAFRMDLDEAPGDKTRVNLPHPEIFEALEPGASLLVNDGKIRLKVEDCSKTHANCTVIAGGVISNRKGV
NVPDVVLPLAALSDKDRKDLEFACELGVDWLALSFVQRADDVLEASDLANGRAAILSKIEKPAAVHNFDEILEVSDGIMV
ARGDLGVELPVQNVPPIQKRLVRKCRRAAKPVIVATQMMESMIESPVPTRAEVSDVATAIYEGADAVMLSAESAAGAYPV
EAVTTMNNVAIEVEQDPTYTQIIEASREAKGRSVADGIVSAAREIAETTNIKAICCFSQSGTTAMLTARERPRVPIIVMT
SKARTARRLTLAWGCNCVMTDQLERFKQAVVNSARVATRQGYATKEDLIVVTAGVPFNVPGSTNILRVAPCDERLIFNTD
PE
>Mature_482_residues
MRRHRNVKIVATLGPASSDYDMIRALHDAGADVFRLNMSHGTHDELRERHRIIRQIEEDTGGSICILADLQGPKLRVGVF
ANEEGEMLEEGAAFRMDLDEAPGDKTRVNLPHPEIFEALEPGASLLVNDGKIRLKVEDCSKTHANCTVIAGGVISNRKGV
NVPDVVLPLAALSDKDRKDLEFACELGVDWLALSFVQRADDVLEASDLANGRAAILSKIEKPAAVHNFDEILEVSDGIMV
ARGDLGVELPVQNVPPIQKRLVRKCRRAAKPVIVATQMMESMIESPVPTRAEVSDVATAIYEGADAVMLSAESAAGAYPV
EAVTTMNNVAIEVEQDPTYTQIIEASREAKGRSVADGIVSAAREIAETTNIKAICCFSQSGTTAMLTARERPRVPIIVMT
SKARTARRLTLAWGCNCVMTDQLERFKQAVVNSARVATRQGYATKEDLIVVTAGVPFNVPGSTNILRVAPCDERLIFNTD
PE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=37.6782077393075, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=37.6782077393075, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=38.1818181818182, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI10835121, Length=500, Percent_Identity=37.4, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI32967597, Length=500, Percent_Identity=37.4, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=37.8472222222222, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=37.8472222222222, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=32.6271186440678, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=32.6271186440678, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI310128738, Length=211, Percent_Identity=32.2274881516588, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=37.2093023255814, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=38.3647798742138, Blast_Score=281, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=424, Percent_Identity=38.9150943396226, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=35.3909465020576, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=35.3909465020576, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=35.3909465020576, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=35.3909465020576, Blast_Score=242, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=494, Percent_Identity=33.8056680161943, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=420, Percent_Identity=37.1428571428571, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24648964, Length=481, Percent_Identity=35.5509355509356, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI28571814, Length=481, Percent_Identity=35.5509355509356, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24648966, Length=400, Percent_Identity=36.25, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24581235, Length=427, Percent_Identity=32.7868852459016, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24646914, Length=249, Percent_Identity=38.1526104417671, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52343; Mature: 52343

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHRNVKIVATLGPASSDYDMIRALHDAGADVFRLNMSHGTHDELRERHRIIRQIEEDT
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
GGSICILADLQGPKLRVGVFANEEGEMLEEGAAFRMDLDEAPGDKTRVNLPHPEIFEALE
CCCEEEEEECCCCEEEEEEEECCCCHHHHCCCEEEEECCCCCCCCEEEECCCHHHHHHHC
PGASLLVNDGKIRLKVEDCSKTHANCTVIAGGVISNRKGVNVPDVVLPLAALSDKDRKDL
CCCEEEEECCEEEEEEECCCCCCCCEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCCCH
EFACELGVDWLALSFVQRADDVLEASDLANGRAAILSKIEKPAAVHNFDEILEVSDGIMV
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCHHHHHHHCCCEEE
ARGDLGVELPVQNVPPIQKRLVRKCRRAAKPVIVATQMMESMIESPVPTRAEVSDVATAI
EECCCCEECCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
YEGADAVMLSAESAAGAYPVEAVTTMNNVAIEVEQDPTYTQIIEASREAKGRSVADGIVS
HCCCCEEEEEECCCCCCCCHHHEEECCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
AAREIAETTNIKAICCFSQSGTTAMLTARERPRVPIIVMTSKARTARRLTLAWGCNCVMT
HHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCEEEH
DQLERFKQAVVNSARVATRQGYATKEDLIVVTAGVPFNVPGSTNILRVAPCDERLIFNTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCEEECCC
PE
CC
>Mature Secondary Structure
MRRHRNVKIVATLGPASSDYDMIRALHDAGADVFRLNMSHGTHDELRERHRIIRQIEEDT
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
GGSICILADLQGPKLRVGVFANEEGEMLEEGAAFRMDLDEAPGDKTRVNLPHPEIFEALE
CCCEEEEEECCCCEEEEEEEECCCCHHHHCCCEEEEECCCCCCCCEEEECCCHHHHHHHC
PGASLLVNDGKIRLKVEDCSKTHANCTVIAGGVISNRKGVNVPDVVLPLAALSDKDRKDL
CCCEEEEECCEEEEEEECCCCCCCCEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCCCH
EFACELGVDWLALSFVQRADDVLEASDLANGRAAILSKIEKPAAVHNFDEILEVSDGIMV
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCHHHHHHHCCCEEE
ARGDLGVELPVQNVPPIQKRLVRKCRRAAKPVIVATQMMESMIESPVPTRAEVSDVATAI
EECCCCEECCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
YEGADAVMLSAESAAGAYPVEAVTTMNNVAIEVEQDPTYTQIIEASREAKGRSVADGIVS
HCCCCEEEEEECCCCCCCCHHHEEECCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHH
AAREIAETTNIKAICCFSQSGTTAMLTARERPRVPIIVMTSKARTARRLTLAWGCNCVMT
HHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCEEEH
DQLERFKQAVVNSARVATRQGYATKEDLIVVTAGVPFNVPGSTNILRVAPCDERLIFNTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCEEECCC
PE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA