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The map label for this gene is ilvE [H]

Identifier: 110678335

GI number: 110678335

Start: 955308

End: 956246

Strand: Direct

Name: ilvE [H]

Synonym: RD1_0990

Alternate gene names: 110678335

Gene position: 955308-956246 (Clockwise)

Preceding gene: 110678333

Following gene: 110678337

Centisome position: 23.11

GC content: 59.64

Gene sequence:

>939_bases
ATGGATACAGAGACCCTGACCACCCATGATGCCGATGATGACCCGCGCAACCGCGACATCAAGATCTACATCAATGGGCA
GCTTTTGCACCGTGATGCCGCCAAGGTGTCGGTCTATGATGCGGGCTTCATGCTGGGCGACGGGATGTGGGAAGGCATGC
GGCTCTATGACGGGGTCTGGAGCTTTTTCGACGCGCATATGGATCGGTTTTTTGACAGCTGCAAGGCGGTCTCTCTGGAT
GTGGGGATGGACCGCAACGGGATCATGGAGGCGCTCAAACAGACGGCCAGGGCCAACAATATGGTCACGGATGTTCATTG
CCGTTTGATGCTGACGCGGGGCCTCAAGGCGAAACCCTTTCAGCACCCGTCCCTGAGCCGCACCGGCCCAACGCTGGTCA
TCATCATGGAACATTCCAAGCCCGCGACCGCCTTGCAGGATCGCGGCATTCGTCTGGCCACCGTGCCGCAGGTGCGCGGT
TTGCCGATGTCGCAGGATGCCAAGTACAACAGTCATTCCAAGCTGAACTGCGTCATCGCCTGTTTGCAGGCGGAGCAGGC
GGGGGCGGATGAGGGGCTGATGCTGGACCCGCATGGGTTTGTGAACACCACCAATGCCTGCAATTTCTTTATCGTGCGGC
GGGGCGAGGTGTGGACCTCCACCGGTGATTACTGCATGAACGGCGTTACCCGGCAGACGGTGATTGATATCTGCCGCGCC
GAGGGCATCCCGGTTTTCGAAAAGAACTATTCGCTTTATGAGGCATATGGCGCGCAGGAGGCGTTTCTGACCGGGACATT
CGGGGCGCAGACCCCGGTTGCGGAAATTGACGGCAAGCCGATCGGGGATGGCACGCGGCCCGTTACGCAGCGCATTCGCG
AAGTCTACAAGCAATGGATCGCCAAGGACGTGGCCGAACAGGCCCATCGCTGGACCTGA

Upstream 100 bases:

>100_bases
GGTGTCGCAGGGCAACGACACTCTGGTGGCGCGCCGGGCTGGGCTTGCGCAACCCTTTGCATTCTGATTGCGTGCGCAAA
AATGAGCCGAGGACACCGAT

Downstream 100 bases:

>100_bases
GCGCGCCTATTCGGGCGTTGTGGCCGATTGCAGAAAGCTGATCAGGTCGGCGCGGTCCTGCGCGCCCGTGATCCGTTGCA
TCGGCATCTTGGAGCCGGGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDTETLTTHDADDDPRNRDIKIYINGQLLHRDAAKVSVYDAGFMLGDGMWEGMRLYDGVWSFFDAHMDRFFDSCKAVSLD
VGMDRNGIMEALKQTARANNMVTDVHCRLMLTRGLKAKPFQHPSLSRTGPTLVIIMEHSKPATALQDRGIRLATVPQVRG
LPMSQDAKYNSHSKLNCVIACLQAEQAGADEGLMLDPHGFVNTTNACNFFIVRRGEVWTSTGDYCMNGVTRQTVIDICRA
EGIPVFEKNYSLYEAYGAQEAFLTGTFGAQTPVAEIDGKPIGDGTRPVTQRIREVYKQWIAKDVAEQAHRWT

Sequences:

>Translated_312_residues
MDTETLTTHDADDDPRNRDIKIYINGQLLHRDAAKVSVYDAGFMLGDGMWEGMRLYDGVWSFFDAHMDRFFDSCKAVSLD
VGMDRNGIMEALKQTARANNMVTDVHCRLMLTRGLKAKPFQHPSLSRTGPTLVIIMEHSKPATALQDRGIRLATVPQVRG
LPMSQDAKYNSHSKLNCVIACLQAEQAGADEGLMLDPHGFVNTTNACNFFIVRRGEVWTSTGDYCMNGVTRQTVIDICRA
EGIPVFEKNYSLYEAYGAQEAFLTGTFGAQTPVAEIDGKPIGDGTRPVTQRIREVYKQWIAKDVAEQAHRWT
>Mature_312_residues
MDTETLTTHDADDDPRNRDIKIYINGQLLHRDAAKVSVYDAGFMLGDGMWEGMRLYDGVWSFFDAHMDRFFDSCKAVSLD
VGMDRNGIMEALKQTARANNMVTDVHCRLMLTRGLKAKPFQHPSLSRTGPTLVIIMEHSKPATALQDRGIRLATVPQVRG
LPMSQDAKYNSHSKLNCVIACLQAEQAGADEGLMLDPHGFVNTTNACNFFIVRRGEVWTSTGDYCMNGVTRQTVIDICRA
EGIPVFEKNYSLYEAYGAQEAFLTGTFGAQTPVAEIDGKPIGDGTRPVTQRIREVYKQWIAKDVAEQAHRWT

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=282, Percent_Identity=27.3049645390071, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787338, Length=253, Percent_Identity=28.0632411067194, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34861; Mature: 34861

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTETLTTHDADDDPRNRDIKIYINGQLLHRDAAKVSVYDAGFMLGDGMWEGMRLYDGVW
CCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCEEEEEECCEEECCCCHHHHHHHHHHH
SFFDAHMDRFFDSCKAVSLDVGMDRNGIMEALKQTARANNMVTDVHCRLMLTRGLKAKPF
HHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCC
QHPSLSRTGPTLVIIMEHSKPATALQDRGIRLATVPQVRGLPMSQDAKYNSHSKLNCVIA
CCCCCCCCCCEEEEEEECCCCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
CLQAEQAGADEGLMLDPHGFVNTTNACNFFIVRRGEVWTSTGDYCMNGVTRQTVIDICRA
EEHHHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCEECCCCHHHHCCCHHHHHHHHHHC
EGIPVFEKNYSLYEAYGAQEAFLTGTFGAQTPVAEIDGKPIGDGTRPVTQRIREVYKQWI
CCCCEEECCCCHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AKDVAEQAHRWT
HHHHHHHHHCCC
>Mature Secondary Structure
MDTETLTTHDADDDPRNRDIKIYINGQLLHRDAAKVSVYDAGFMLGDGMWEGMRLYDGVW
CCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCEEEEEECCEEECCCCHHHHHHHHHHH
SFFDAHMDRFFDSCKAVSLDVGMDRNGIMEALKQTARANNMVTDVHCRLMLTRGLKAKPF
HHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCC
QHPSLSRTGPTLVIIMEHSKPATALQDRGIRLATVPQVRGLPMSQDAKYNSHSKLNCVIA
CCCCCCCCCCEEEEEEECCCCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
CLQAEQAGADEGLMLDPHGFVNTTNACNFFIVRRGEVWTSTGDYCMNGVTRQTVIDICRA
EEHHHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCEECCCCHHHHCCCHHHHHHHHHHC
EGIPVFEKNYSLYEAYGAQEAFLTGTFGAQTPVAEIDGKPIGDGTRPVTQRIREVYKQWI
CCCCEEECCCCHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AKDVAEQAHRWT
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]