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The map label for this gene is dapA

Identifier: 110678318

GI number: 110678318

Start: 935530

End: 936405

Strand: Direct

Name: dapA

Synonym: RD1_0972

Alternate gene names: 110678318

Gene position: 935530-936405 (Clockwise)

Preceding gene: 110678316

Following gene: 110678322

Centisome position: 22.64

GC content: 59.02

Gene sequence:

>876_bases
ATGTTCAAAGGTTCTATGCCTGCCCTCGTCACGCCGTTTCGGAACGGCGAGCTGGATCTGGAAACGCTTAAAAAGCTGGT
GGAATGGCACATCGGTGAGGGCAGCAACGGTCTGGTACCGGTCGGTACAACCGGTGAAAGCCCGACACTGACGCACAAGG
AACATGAGGTCGTCGTCGAAGAAGTGGTCAAGGCTGCGGCAGGCCGTGTTCCCGTGATTGCCGGTGCCGGGTCCAACAAC
ACGCTTGAGGCGATCCGCCTTGTGCGCCATGCGGAGGCCGTCGGTGCGGATGCCGCGCTCGTGGTGACGCCCTATTACAA
CAAGCCCACGCAATCAGGATTGATTGCGCATTTCACGGCGCTGCATGACTGCGCGGATCTGCCGATCATTATCTATAATA
TTCCGGGCCGCTCAGTCGTGGATATGAGCCCGGAAACCATGGGTAAACTGGCCGAACTGCCGCGCATCATCGGCGTCAAG
GACGCGACAGGTGATCTGGCGCGTGTCTGCGATCAGCGCAGCACCTGTGGCCCGGATTTCATGCAGCTCTCCGGTGAAGA
CGCAACAGCGCACGGGTTTAATGCACAAGGCGGCGTTGGTTGCATTTCGGTGACGGCGAATGTTGCGCCCAAACTGTTGA
GTCAGATGCAGGCGGCGTGTCTGGCCGGTGATTATGCAACCGCGCTGAGCATTCAGGACCGGTTGATGCCCCTGCACAAG
GCGATCTTTATCGAACCCGGCCTTGTGGGGGTCAAATATGCGATGTCACAGCTTGATCTGTGTTCGGAAGAGGTACGCCT
GCCGCTGACGGCCTTGTCCGATGAAACCCGCGCACTGGTCGATGCGGGGCTGAGGCATGCCGGTCTGATGAACTAG

Upstream 100 bases:

>100_bases
CTCATACTTATTGCGTCGATGATAGGGATCAAATCACGATCCTGCTTACATCGGGTTAACCCAGGCCATTTGCCGGCCAT
AATGCTAAAGGAGCGTGAAT

Downstream 100 bases:

>100_bases
GGCGTCTGACCAGATACCTGACATATCAATATCACGTAACGCATTGAAACCTTTGATTTCAGTCAGCGTTACGTGAGCCA
GGGTTTTCAGCGCTTGCCGT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MFKGSMPALVTPFRNGELDLETLKKLVEWHIGEGSNGLVPVGTTGESPTLTHKEHEVVVEEVVKAAAGRVPVIAGAGSNN
TLEAIRLVRHAEAVGADAALVVTPYYNKPTQSGLIAHFTALHDCADLPIIIYNIPGRSVVDMSPETMGKLAELPRIIGVK
DATGDLARVCDQRSTCGPDFMQLSGEDATAHGFNAQGGVGCISVTANVAPKLLSQMQAACLAGDYATALSIQDRLMPLHK
AIFIEPGLVGVKYAMSQLDLCSEEVRLPLTALSDETRALVDAGLRHAGLMN

Sequences:

>Translated_291_residues
MFKGSMPALVTPFRNGELDLETLKKLVEWHIGEGSNGLVPVGTTGESPTLTHKEHEVVVEEVVKAAAGRVPVIAGAGSNN
TLEAIRLVRHAEAVGADAALVVTPYYNKPTQSGLIAHFTALHDCADLPIIIYNIPGRSVVDMSPETMGKLAELPRIIGVK
DATGDLARVCDQRSTCGPDFMQLSGEDATAHGFNAQGGVGCISVTANVAPKLLSQMQAACLAGDYATALSIQDRLMPLHK
AIFIEPGLVGVKYAMSQLDLCSEEVRLPLTALSDETRALVDAGLRHAGLMN
>Mature_291_residues
MFKGSMPALVTPFRNGELDLETLKKLVEWHIGEGSNGLVPVGTTGESPTLTHKEHEVVVEEVVKAAAGRVPVIAGAGSNN
TLEAIRLVRHAEAVGADAALVVTPYYNKPTQSGLIAHFTALHDCADLPIIIYNIPGRSVVDMSPETMGKLAELPRIIGVK
DATGDLARVCDQRSTCGPDFMQLSGEDATAHGFNAQGGVGCISVTANVAPKLLSQMQAACLAGDYATALSIQDRLMPLHK
AIFIEPGLVGVKYAMSQLDLCSEEVRLPLTALSDETRALVDAGLRHAGLMN

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=278, Percent_Identity=30.9352517985612, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI13540533, Length=242, Percent_Identity=28.9256198347107, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1788823, Length=292, Percent_Identity=47.2602739726027, Blast_Score=263, Evalue=7e-72,
Organism=Escherichia coli, GI87082415, Length=296, Percent_Identity=32.0945945945946, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1786463, Length=287, Percent_Identity=31.3588850174216, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1789620, Length=264, Percent_Identity=31.8181818181818, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_ROSDO (Q16BK4)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681325.1
- ProteinModelPortal:   Q16BK4
- SMR:   Q16BK4
- STRING:   Q16BK4
- GeneID:   4197965
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_0972
- NMPDR:   fig|375451.6.peg.911
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   SGEDMTA
- PhylomeDB:   Q16BK4
- ProtClustDB:   PRK03170
- BioCyc:   RDEN375451:RD1_0972-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 30744; Mature: 30744

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 160-160 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGSMPALVTPFRNGELDLETLKKLVEWHIGEGSNGLVPVGTTGESPTLTHKEHEVVVE
CCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
EVVKAAAGRVPVIAGAGSNNTLEAIRLVRHAEAVGADAALVVTPYYNKPTQSGLIAHFTA
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
LHDCADLPIIIYNIPGRSVVDMSPETMGKLAELPRIIGVKDATGDLARVCDQRSTCGPDF
HHHHCCCCEEEEECCCCCEECCCHHHHHHHHHCHHHEECCCCCCHHHHHHCCCCCCCCHH
MQLSGEDATAHGFNAQGGVGCISVTANVAPKLLSQMQAACLAGDYATALSIQDRLMPLHK
HEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIFIEPGLVGVKYAMSQLDLCSEEVRLPLTALSDETRALVDAGLRHAGLMN
HEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFKGSMPALVTPFRNGELDLETLKKLVEWHIGEGSNGLVPVGTTGESPTLTHKEHEVVVE
CCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
EVVKAAAGRVPVIAGAGSNNTLEAIRLVRHAEAVGADAALVVTPYYNKPTQSGLIAHFTA
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
LHDCADLPIIIYNIPGRSVVDMSPETMGKLAELPRIIGVKDATGDLARVCDQRSTCGPDF
HHHHCCCCEEEEECCCCCEECCCHHHHHHHHHCHHHEECCCCCCHHHHHHCCCCCCCCHH
MQLSGEDATAHGFNAQGGVGCISVTANVAPKLLSQMQAACLAGDYATALSIQDRLMPLHK
HEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIFIEPGLVGVKYAMSQLDLCSEEVRLPLTALSDETRALVDAGLRHAGLMN
HEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA