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The map label for this gene is mnmC [H]

Identifier: 110678316

GI number: 110678316

Start: 932727

End: 933428

Strand: Direct

Name: mnmC [H]

Synonym: RD1_0970

Alternate gene names: 110678316

Gene position: 932727-933428 (Clockwise)

Preceding gene: 110678314

Following gene: 110678318

Centisome position: 22.57

GC content: 56.41

Gene sequence:

>702_bases
ATGCAGAACCAGACCGCAGATGTAACCTGGCAGTCGGAAGATGTTCCGGTATCGACCAGATTTGATGATCCCTACTACAG
CCTGGAAAATGGCTTGGCTGAAACACGTCATGTTTTCCTTGCCGGAAACCGTTTGCCTGAACGGTTTGGTGGGTCGTTTC
GTATCGCTGAGCTTGGGTTTGGCACGGGGATGAATTTTCTGGCCGCAGCTCAGATGTGGCAGGCGCAGGGGGTGTGTGGG
CAGATGCGGTTCACCAGCTTTGAAGCCTATCCGATGGCACGGGCAGATATGTTCAGGGCATTGAGCGTTTATAGGGAAAC
GGATCTGCCTGTTGATCGCCTTATTGACATGTGGTCGCCGGAGGGGGGTGTTTTTGAGTTTGCGCCCGACATCCAGCTTG
AGGTCATAATCGGGGATGCGCGAAACACTGTGGCTCAGTGGCAGGGGCACGCGGATGCATGGTTTCTGGATGGTTTTTCG
CCCGCGAAAAACCCTGAATTATGGAGTGAGGCGCTTTTGCATGATGTCGCTTCTCATACCGCGCCCGGTGGCACTTTCGC
GACCTATACAGCCGCCGGTTTTGTTCGCCGCGCGCTGGGTGCGGCGGGTTTTGATGTGCAGCGCATCGCGGGATATGGCC
GGAAAAGGCACATGAGTTGCGGGGTTTTGCGCGATCCCGCTCAACGGGCCACCGGTTGGTGA

Upstream 100 bases:

>100_bases
CAAGAATACCCGCCCCGCGAATGGTGATATCAATCATTGCCAATTTGTGCTTCCTGCCTCGGCGTTGTATTTGTCTTGGA
CCCCTTTACAAGGCGCGCGC

Downstream 100 bases:

>100_bases
GTCACTCTCCTTTCGGTGCGTAGTCTAATAGGGTCGACCCCGTCAGTTCCTTGCTGAAGGGAATGGGAAGCGGATTTTTT
CCTAGCTGGGCGCGGTAGAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MQNQTADVTWQSEDVPVSTRFDDPYYSLENGLAETRHVFLAGNRLPERFGGSFRIAELGFGTGMNFLAAAQMWQAQGVCG
QMRFTSFEAYPMARADMFRALSVYRETDLPVDRLIDMWSPEGGVFEFAPDIQLEVIIGDARNTVAQWQGHADAWFLDGFS
PAKNPELWSEALLHDVASHTAPGGTFATYTAAGFVRRALGAAGFDVQRIAGYGRKRHMSCGVLRDPAQRATGW

Sequences:

>Translated_233_residues
MQNQTADVTWQSEDVPVSTRFDDPYYSLENGLAETRHVFLAGNRLPERFGGSFRIAELGFGTGMNFLAAAQMWQAQGVCG
QMRFTSFEAYPMARADMFRALSVYRETDLPVDRLIDMWSPEGGVFEFAPDIQLEVIIGDARNTVAQWQGHADAWFLDGFS
PAKNPELWSEALLHDVASHTAPGGTFATYTAAGFVRRALGAAGFDVQRIAGYGRKRHMSCGVLRDPAQRATGW
>Mature_233_residues
MQNQTADVTWQSEDVPVSTRFDDPYYSLENGLAETRHVFLAGNRLPERFGGSFRIAELGFGTGMNFLAAAQMWQAQGVCG
QMRFTSFEAYPMARADMFRALSVYRETDLPVDRLIDMWSPEGGVFEFAPDIQLEVIIGDARNTVAQWQGHADAWFLDGFS
PAKNPELWSEALLHDVASHTAPGGTFATYTAAGFVRRALGAAGFDVQRIAGYGRKRHMSCGVLRDPAQRATGW

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=242, Percent_Identity=42.1487603305785, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 25825; Mature: 25825

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQTADVTWQSEDVPVSTRFDDPYYSLENGLAETRHVFLAGNRLPERFGGSFRIAELGF
CCCCCCCEEECCCCCCEEECCCCCHHHHHCCHHHHEEEEEECCCCHHHHCCCEEEEEECC
GTGMNFLAAAQMWQAQGVCGQMRFTSFEAYPMARADMFRALSVYRETDLPVDRLIDMWSP
CCCHHHHHHHHHHHHCCCCCCCEECEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCC
EGGVFEFAPDIQLEVIIGDARNTVAQWQGHADAWFLDGFSPAKNPELWSEALLHDVASHT
CCCEEEECCCCEEEEEECCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHC
APGGTFATYTAAGFVRRALGAAGFDVQRIAGYGRKRHMSCGVLRDPAQRATGW
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MQNQTADVTWQSEDVPVSTRFDDPYYSLENGLAETRHVFLAGNRLPERFGGSFRIAELGF
CCCCCCCEEECCCCCCEEECCCCCHHHHHCCHHHHEEEEEECCCCHHHHCCCEEEEEECC
GTGMNFLAAAQMWQAQGVCGQMRFTSFEAYPMARADMFRALSVYRETDLPVDRLIDMWSP
CCCHHHHHHHHHHHHCCCCCCCEECEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCC
EGGVFEFAPDIQLEVIIGDARNTVAQWQGHADAWFLDGFSPAKNPELWSEALLHDVASHT
CCCEEEECCCCEEEEEECCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHC
APGGTFATYTAAGFVRRALGAAGFDVQRIAGYGRKRHMSCGVLRDPAQRATGW
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA