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The map label for this gene is mscS [H]

Identifier: 110678292

GI number: 110678292

Start: 914209

End: 915054

Strand: Direct

Name: mscS [H]

Synonym: RD1_0945

Alternate gene names: 110678292

Gene position: 914209-915054 (Clockwise)

Preceding gene: 110678291

Following gene: 110678294

Centisome position: 22.12

GC content: 56.5

Gene sequence:

>846_bases
ATGCAGGCACTATTGCAAACAGAAGTATGGCAGGGAAAGACGGTCGCGGATTTGCTGTCGGTCGAATTCCTGATGTCTGC
GGTCGGGTCCGTCGTGGCGGCCATTGCGATACTGATCCTCGGCTGGATTGTTTCGGCATGGCTTCAGGCACGGGTGCGCG
CGCTCGGGCGCAAACACAAGAATCTTGATGACATGCTATTCGACTTCTTGTCGGCCATCGTCCGCTATGTGGTGCTTGGC
TTTGCCATCCTGTTTGTCCTGAATACATTTGGCGTGCAGACCACATCGGTGGTTGCGGTTATCGGTGCGGCCGGTCTTGC
GGTTGGTCTGGCCTTGCAGGGCACTTTGTCCAATATCGCGGCGGGCGTCATGCTGATCCTGTTCAGGCCGATCAAACTGG
GCGATTTTGTCGATGTCGGCGGGACAATGGGCACGGTTCAATCCCTGACACTGAATTTTACCGAACTTGCAGATATCAGC
AACGCGCAGGTTATCATCCCGAACGCGCAGGTCTGGGGCAATGTGATCACGAATTACTCTGCCAACGACACGCGCCGCGC
CGAATGGACCTTTGGTGTGGGCTATGGCGTGAACCTCGCAGAGGCCGAGCGTATCATCCGCGAGACGATCATGGCCGATC
CGCGGTCCAAAACAGACCCGGAACCCTTTATTCAGGTCAATAACCTCGGCGAAAGCTCGGTCGATTTTCTGGTGCGGGTC
TGGTGTGATGCGGGTGAATTGTTCGGCTATCAGGCCGATATGAAGCGCAAGGTCAAAGAGGCCTTGGATGCCGGTGGTGT
GGACATCCCGTTCCCCACAACAACCATCGTTCAGGCGGCCGGTTAA

Upstream 100 bases:

>100_bases
AACGGTGAATGTGGTGCTGACCACGCATGATGTGGGTGGGCTGAGCACATTGGATGCGAAACTGGCCCGTAAAATGGACA
GTTTGGCAGGATAACATAAT

Downstream 100 bases:

>100_bases
ACCCGGATGCAGCAAAGCCCGGGCACAGGGGGTGCCCGGGCTTTTTTCGTTTACATATTCGCCAAAAGGGCTTCACCGGC
GGAAACTTCGCAGGTGCCGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQALLQTEVWQGKTVADLLSVEFLMSAVGSVVAAIAILILGWIVSAWLQARVRALGRKHKNLDDMLFDFLSAIVRYVVLG
FAILFVLNTFGVQTTSVVAVIGAAGLAVGLALQGTLSNIAAGVMLILFRPIKLGDFVDVGGTMGTVQSLTLNFTELADIS
NAQVIIPNAQVWGNVITNYSANDTRRAEWTFGVGYGVNLAEAERIIRETIMADPRSKTDPEPFIQVNNLGESSVDFLVRV
WCDAGELFGYQADMKRKVKEALDAGGVDIPFPTTTIVQAAG

Sequences:

>Translated_281_residues
MQALLQTEVWQGKTVADLLSVEFLMSAVGSVVAAIAILILGWIVSAWLQARVRALGRKHKNLDDMLFDFLSAIVRYVVLG
FAILFVLNTFGVQTTSVVAVIGAAGLAVGLALQGTLSNIAAGVMLILFRPIKLGDFVDVGGTMGTVQSLTLNFTELADIS
NAQVIIPNAQVWGNVITNYSANDTRRAEWTFGVGYGVNLAEAERIIRETIMADPRSKTDPEPFIQVNNLGESSVDFLVRV
WCDAGELFGYQADMKRKVKEALDAGGVDIPFPTTTIVQAAG
>Mature_281_residues
MQALLQTEVWQGKTVADLLSVEFLMSAVGSVVAAIAILILGWIVSAWLQARVRALGRKHKNLDDMLFDFLSAIVRYVVLG
FAILFVLNTFGVQTTSVVAVIGAAGLAVGLALQGTLSNIAAGVMLILFRPIKLGDFVDVGGTMGTVQSLTLNFTELADIS
NAQVIIPNAQVWGNVITNYSANDTRRAEWTFGVGYGVNLAEAERIIRETIMADPRSKTDPEPFIQVNNLGESSVDFLVRV
WCDAGELFGYQADMKRKVKEALDAGGVDIPFPTTTIVQAAG

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=250, Percent_Identity=40.8, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1786670, Length=204, Percent_Identity=24.0196078431373, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787591, Length=214, Percent_Identity=23.8317757009346, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367355, Length=213, Percent_Identity=22.5352112676056, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30222; Mature: 30222

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALLQTEVWQGKTVADLLSVEFLMSAVGSVVAAIAILILGWIVSAWLQARVRALGRKHK
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLDDMLFDFLSAIVRYVVLGFAILFVLNTFGVQTTSVVAVIGAAGLAVGLALQGTLSNIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVMLILFRPIKLGDFVDVGGTMGTVQSLTLNFTELADISNAQVIIPNAQVWGNVITNYS
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCEEEECCHHHHHHHHHCCC
ANDTRRAEWTFGVGYGVNLAEAERIIRETIMADPRSKTDPEPFIQVNNLGESSVDFLVRV
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHH
WCDAGELFGYQADMKRKVKEALDAGGVDIPFPTTTIVQAAG
HHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MQALLQTEVWQGKTVADLLSVEFLMSAVGSVVAAIAILILGWIVSAWLQARVRALGRKHK
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLDDMLFDFLSAIVRYVVLGFAILFVLNTFGVQTTSVVAVIGAAGLAVGLALQGTLSNIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGVMLILFRPIKLGDFVDVGGTMGTVQSLTLNFTELADISNAQVIIPNAQVWGNVITNYS
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCEEEECCHHHHHHHHHCCC
ANDTRRAEWTFGVGYGVNLAEAERIIRETIMADPRSKTDPEPFIQVNNLGESSVDFLVRV
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHH
WCDAGELFGYQADMKRKVKEALDAGGVDIPFPTTTIVQAAG
HHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]