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The map label for this gene is camA [H]

Identifier: 110678294

GI number: 110678294

Start: 915689

End: 916897

Strand: Direct

Name: camA [H]

Synonym: RD1_0947

Alternate gene names: 110678294

Gene position: 915689-916897 (Clockwise)

Preceding gene: 110678292

Following gene: 110678295

Centisome position: 22.16

GC content: 60.71

Gene sequence:

>1209_bases
ATGAGCCATGTCGTGGTGATCGGAGCGGGTCAGGCGGGGTCGTCCTGCGTGGCCAAGCTGCGCAACGCCGGGTTCGATGG
TCAGGTGACCCTGATCGGCGCGGAACCCGTGGCACCCTATCAACGCCCGCCCTTGTCCAAGGCCTATCTGATGGGCGAGA
TGGCGCTTGAGCGTCTGTTCCTGCGGCCGGAGCGGTTTTATGCGGATAATGACATTGACCTGCGTCTGAACACGGTTGTC
GAAGCGGTTGAGTGCGGTGCGCGCAAGGTCCATCTGGCGGGTGAAACGCTGGACTTCGACGATCTGGTGTTCACGACAGG
CTCCACCCCGCGCCGTCTGCCTGCTGCGATTGGCGGCGATCTTGGCCATGTCTTTGTGATGCGCGATCTGGCGGATGCGG
ATGCGATGACCTCGCATTTTCGCGCAGGCGCCCGGGTCTTGATCGTTGGTGGCGGCTACATCGGGCTGGAGGCCGCAGCG
GTGGCGGCCAAACTGGGGCTGTGCGTGACGCTGGTGGAGATGTCCGAACGCATCTTGCAACGCGTCGCAGCCCCGGAGAC
AAGCGCGTATTTTCGCAGTTTGCACAGCGCGCATGGCGTCGATCTGCGCGAAGGTGTCGGGCTGGAACGCCTGATCGGGG
ATGAAACGGTGACTGGGGCACGGCTGTCGGACGGCTCCGAAATCGCGGTGGATTACGTGATCGTCGGGGTTGGTATCGCG
CCGAATACGGGCTTGGCCGAAGCCGCCGGACTTAAGATCGACAATGGCATTGCAACCGATGTGCATGGCCGCACGTCTGT
GCCGCATGTCTGGGCGGCGGGGGATTGCGCGTCTTTTCCCTATCAGCAAGCGCGTATCCGGCTCGAAAGCGTGCCGCATG
CCATTGATCAGGCGGAAACCGTAGCTGAAAACATCATGGGCGCTGAAAAGGAATATACCGCCAAACCATGGTTCTGGTCG
GATCAGTACGATGTAAAGTTACAGATTGCTGGGCTGAATACTGGATATACAGATGTTGTGCCGCGCATTGGTGAGGGGCA
AACGGCGTCGTTCTGGTATTACAGTGGCGACAGGCTTTTGGCCGTGGATGCGATGAACGACCCGCGTGCCTATATGATCG
GGAAGCGATTGATCGAGGCGGGGAAAACGGCGGATCGCGCCATCGTTGCGGACTCATCGGCAGACCTAAAGCCGCTGTTG
CAGGGGTGA

Upstream 100 bases:

>100_bases
GTCATGTGCTACGCCTTTCAGGTATTTGCGTTTGGTCCTCGCAAAGATATAGGGTGCTGCGTCTCAGGCGACCAGTAGTT
TCCGAAAGAGGGGGGATAAG

Downstream 100 bases:

>100_bases
GAATTATCGCCGGCCAGTTTCGCAGCGTCACGCTTGCCAGTGTGGGCAAGGGCGACGCCGCTGCACACCTGCGCCCAACG
ACGGACCGCGTGCGCGAAAG

Product: pyridine nucleotide-disulphide oxidoreductase, putative

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSHVVVIGAGQAGSSCVAKLRNAGFDGQVTLIGAEPVAPYQRPPLSKAYLMGEMALERLFLRPERFYADNDIDLRLNTVV
EAVECGARKVHLAGETLDFDDLVFTTGSTPRRLPAAIGGDLGHVFVMRDLADADAMTSHFRAGARVLIVGGGYIGLEAAA
VAAKLGLCVTLVEMSERILQRVAAPETSAYFRSLHSAHGVDLREGVGLERLIGDETVTGARLSDGSEIAVDYVIVGVGIA
PNTGLAEAAGLKIDNGIATDVHGRTSVPHVWAAGDCASFPYQQARIRLESVPHAIDQAETVAENIMGAEKEYTAKPWFWS
DQYDVKLQIAGLNTGYTDVVPRIGEGQTASFWYYSGDRLLAVDAMNDPRAYMIGKRLIEAGKTADRAIVADSSADLKPLL
QG

Sequences:

>Translated_402_residues
MSHVVVIGAGQAGSSCVAKLRNAGFDGQVTLIGAEPVAPYQRPPLSKAYLMGEMALERLFLRPERFYADNDIDLRLNTVV
EAVECGARKVHLAGETLDFDDLVFTTGSTPRRLPAAIGGDLGHVFVMRDLADADAMTSHFRAGARVLIVGGGYIGLEAAA
VAAKLGLCVTLVEMSERILQRVAAPETSAYFRSLHSAHGVDLREGVGLERLIGDETVTGARLSDGSEIAVDYVIVGVGIA
PNTGLAEAAGLKIDNGIATDVHGRTSVPHVWAAGDCASFPYQQARIRLESVPHAIDQAETVAENIMGAEKEYTAKPWFWS
DQYDVKLQIAGLNTGYTDVVPRIGEGQTASFWYYSGDRLLAVDAMNDPRAYMIGKRLIEAGKTADRAIVADSSADLKPLL
QG
>Mature_401_residues
SHVVVIGAGQAGSSCVAKLRNAGFDGQVTLIGAEPVAPYQRPPLSKAYLMGEMALERLFLRPERFYADNDIDLRLNTVVE
AVECGARKVHLAGETLDFDDLVFTTGSTPRRLPAAIGGDLGHVFVMRDLADADAMTSHFRAGARVLIVGGGYIGLEAAAV
AAKLGLCVTLVEMSERILQRVAAPETSAYFRSLHSAHGVDLREGVGLERLIGDETVTGARLSDGSEIAVDYVIVGVGIAP
NTGLAEAAGLKIDNGIATDVHGRTSVPHVWAAGDCASFPYQQARIRLESVPHAIDQAETVAENIMGAEKEYTAKPWFWSD
QYDVKLQIAGLNTGYTDVVPRIGEGQTASFWYYSGDRLLAVDAMNDPRAYMIGKRLIEAGKTADRAIVADSSADLKPLLQ
G

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=377, Percent_Identity=29.4429708222812, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI65787454, Length=377, Percent_Identity=29.4429708222812, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI226437568, Length=377, Percent_Identity=29.4429708222812, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI22202629, Length=331, Percent_Identity=24.773413897281, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI4757732, Length=331, Percent_Identity=24.773413897281, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI22202631, Length=107, Percent_Identity=33.6448598130841, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI195927004, Length=107, Percent_Identity=33.6448598130841, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=31.25, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789065, Length=309, Percent_Identity=29.7734627831715, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789765, Length=341, Percent_Identity=28.4457478005865, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=373, Percent_Identity=27.6139410187668, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24585130, Length=403, Percent_Identity=26.302729528536, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24639257, Length=398, Percent_Identity=26.3819095477387, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281359715, Length=398, Percent_Identity=26.3819095477387, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281359713, Length=398, Percent_Identity=26.3819095477387, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24639250, Length=398, Percent_Identity=26.3819095477387, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18543267, Length=398, Percent_Identity=26.3819095477387, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24639252, Length=398, Percent_Identity=26.3819095477387, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28573993, Length=358, Percent_Identity=27.3743016759777, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24581020, Length=358, Percent_Identity=27.3743016759777, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 42943; Mature: 42812

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVVVIGAGQAGSSCVAKLRNAGFDGQVTLIGAEPVAPYQRPPLSKAYLMGEMALERLF
CCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LRPERFYADNDIDLRLNTVVEAVECGARKVHLAGETLDFDDLVFTTGSTPRRLPAAIGGD
CCCHHHCCCCCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCCCCHHHCCC
LGHVFVMRDLADADAMTSHFRAGARVLIVGGGYIGLEAAAVAAKLGLCVTLVEMSERILQ
CCEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAAPETSAYFRSLHSAHGVDLREGVGLERLIGDETVTGARLSDGSEIAVDYVIVGVGIA
HHCCCCHHHHHHHHHHHCCCCHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCC
PNTGLAEAAGLKIDNGIATDVHGRTSVPHVWAAGDCASFPYQQARIRLESVPHAIDQAET
CCCCCHHHCCEEECCCCEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHH
VAENIMGAEKEYTAKPWFWSDQYDVKLQIAGLNTGYTDVVPRIGEGQTASFWYYSGDRLL
HHHHHHCCCCCCCCCCCEECCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEEECCEEE
AVDAMNDPRAYMIGKRLIEAGKTADRAIVADSSADLKPLLQG
EEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCC
>Mature Secondary Structure 
SHVVVIGAGQAGSSCVAKLRNAGFDGQVTLIGAEPVAPYQRPPLSKAYLMGEMALERLF
CEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LRPERFYADNDIDLRLNTVVEAVECGARKVHLAGETLDFDDLVFTTGSTPRRLPAAIGGD
CCCHHHCCCCCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCCCCHHHCCC
LGHVFVMRDLADADAMTSHFRAGARVLIVGGGYIGLEAAAVAAKLGLCVTLVEMSERILQ
CCEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAAPETSAYFRSLHSAHGVDLREGVGLERLIGDETVTGARLSDGSEIAVDYVIVGVGIA
HHCCCCHHHHHHHHHHHCCCCHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCC
PNTGLAEAAGLKIDNGIATDVHGRTSVPHVWAAGDCASFPYQQARIRLESVPHAIDQAET
CCCCCHHHCCEEECCCCEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHH
VAENIMGAEKEYTAKPWFWSDQYDVKLQIAGLNTGYTDVVPRIGEGQTASFWYYSGDRLL
HHHHHHCCCCCCCCCCCEECCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEEECCEEE
AVDAMNDPRAYMIGKRLIEAGKTADRAIVADSSADLKPLLQG
EEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]