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The map label for this gene is livJ [H]

Identifier: 110678205

GI number: 110678205

Start: 831377

End: 832564

Strand: Direct

Name: livJ [H]

Synonym: RD1_0854

Alternate gene names: 110678205

Gene position: 831377-832564 (Clockwise)

Preceding gene: 110678203

Following gene: 110678206

Centisome position: 20.12

GC content: 56.14

Gene sequence:

>1188_bases
ATGAAACGTAGAACACTTATGAAGGCCACGCTGACAGCCGCGTCGCTGATCGCGCTGCAGGCAACCATGGCTGTTGCAGA
AAACCCGGTTCTGAAAATCGGCTTTGTGGGCGTGACCAGCGGCCCTGCTGCTGCATGGGGCATTTCCAACCAGCGTTCGA
TGGAAACACGCGCAGCCTGGATCAACGAAACCGGTGGTTACGAGATCGGCGGTGTTACATATGACATCGAAATCGTATCG
TTTGACGACCAGAAAGACCCCAAGCGCGCCATCGCGGGCATGGAAAAGATGGCCCAGGAAGGTATCCACTACGTTGTCGG
CCCAAATGTTGACGATGGCGCGGCCGCGGTACGTCCGGTGGCAGAAGCCAACGACATCATGTATTTCCCCTATGCTTTCC
CAAGAGAGCTCTATTCCGCGCCTGCATCAAACGCCGTGCTGAGCATGATCGCCAACTACCAGTCGGGTCCTGCGATTTAT
AAGCACCTGATGGAAAACGAAGGTGTCAAAACAGTTGCCTTCATCGCCGCAAACGAATCTGACCCCCTCAGCCAGCGCGA
CAGTGGTGTCGCCGCAGCAAAAGAACTGGGTCTTGAGGTTGTATCCGACAACGTCACCTATCAGGTCGACACGACAGACT
TCACCCCTGTGCTGACACCAATTCTGCGCACGCGCCCTGATCTGCTGGTGCTTTCCGGTGTGTCACCTGCCAATGCCCCG
CAGCTTATCCGCTCCGCACGTGAGCTGGGTTTCGAGGGTCTGATCTCCACTGAAACCGCACAGGACGCTGGCGTTCTGGC
CGAAGGTGCCGGTGATCTGGCCAATGGTTTCATCTCGGTAGGTGGCGCATCCACACCAGAATTGGCGTCCCCGATGATGA
ATGAATTCGTCGAGCGTTACACAGCCATGTTTGGTGAGTACAACGATGAATCCAACACCAAGGTCTACGCGCTCGAGTAT
ATCCTCGAGACACTCAAGGCCACGCCCGAAGCTATTGAGGATGTGGCTGCCTTCCAGGCGTCCATGGACGATTTCTCAGC
GCCAAACCCCTATATGAACGGCGATGAGCAGCTGACCTATGTGGGCATGACCTCCTTCGGTCAAAAGCGTCAGATCGCAG
TGCCGCTGGTTGTCAACGTCTATCAGGACGGTGCCTTCGAGACGATGTTCATCGCGCGCGTCGACTAA

Upstream 100 bases:

>100_bases
CAATCGTGAAGTGACGCCGCGGCACGTGGGCTCATGTGACTCACCGCCACGCAACGAATAGCACACCTAATTCGAAGACG
AATCCAACGGGAGTTAAGAC

Downstream 100 bases:

>100_bases
ACCACTACACACGACATGCTGCTTCCGGCATCGCTCGGAAGCAGCTCCTGCCCGACGGATAGGTCGGGGTTAACCATAGA
AAGAAAGTGCTCATGGAACA

Product: branched-chain amino acid ABC transporter, periplasmic substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKRRTLMKATLTAASLIALQATMAVAENPVLKIGFVGVTSGPAAAWGISNQRSMETRAAWINETGGYEIGGVTYDIEIVS
FDDQKDPKRAIAGMEKMAQEGIHYVVGPNVDDGAAAVRPVAEANDIMYFPYAFPRELYSAPASNAVLSMIANYQSGPAIY
KHLMENEGVKTVAFIAANESDPLSQRDSGVAAAKELGLEVVSDNVTYQVDTTDFTPVLTPILRTRPDLLVLSGVSPANAP
QLIRSARELGFEGLISTETAQDAGVLAEGAGDLANGFISVGGASTPELASPMMNEFVERYTAMFGEYNDESNTKVYALEY
ILETLKATPEAIEDVAAFQASMDDFSAPNPYMNGDEQLTYVGMTSFGQKRQIAVPLVVNVYQDGAFETMFIARVD

Sequences:

>Translated_395_residues
MKRRTLMKATLTAASLIALQATMAVAENPVLKIGFVGVTSGPAAAWGISNQRSMETRAAWINETGGYEIGGVTYDIEIVS
FDDQKDPKRAIAGMEKMAQEGIHYVVGPNVDDGAAAVRPVAEANDIMYFPYAFPRELYSAPASNAVLSMIANYQSGPAIY
KHLMENEGVKTVAFIAANESDPLSQRDSGVAAAKELGLEVVSDNVTYQVDTTDFTPVLTPILRTRPDLLVLSGVSPANAP
QLIRSARELGFEGLISTETAQDAGVLAEGAGDLANGFISVGGASTPELASPMMNEFVERYTAMFGEYNDESNTKVYALEY
ILETLKATPEAIEDVAAFQASMDDFSAPNPYMNGDEQLTYVGMTSFGQKRQIAVPLVVNVYQDGAFETMFIARVD
>Mature_395_residues
MKRRTLMKATLTAASLIALQATMAVAENPVLKIGFVGVTSGPAAAWGISNQRSMETRAAWINETGGYEIGGVTYDIEIVS
FDDQKDPKRAIAGMEKMAQEGIHYVVGPNVDDGAAAVRPVAEANDIMYFPYAFPRELYSAPASNAVLSMIANYQSGPAIY
KHLMENEGVKTVAFIAANESDPLSQRDSGVAAAKELGLEVVSDNVTYQVDTTDFTPVLTPILRTRPDLLVLSGVSPANAP
QLIRSARELGFEGLISTETAQDAGVLAEGAGDLANGFISVGGASTPELASPMMNEFVERYTAMFGEYNDESNTKVYALEY
ILETLKATPEAIEDVAAFQASMDDFSAPNPYMNGDEQLTYVGMTSFGQKRQIAVPLVVNVYQDGAFETMFIARVD

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=276, Percent_Identity=27.536231884058, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789867, Length=284, Percent_Identity=26.4084507042254, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42426; Mature: 42426

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTLMKATLTAASLIALQATMAVAENPVLKIGFVGVTSGPAAAWGISNQRSMETRAAW
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
INETGGYEIGGVTYDIEIVSFDDQKDPKRAIAGMEKMAQEGIHYVVGPNVDDGAAAVRPV
HCCCCCEEECCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCC
AEANDIMYFPYAFPRELYSAPASNAVLSMIANYQSGPAIYKHLMENEGVKTVAFIAANES
CCCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEECCC
DPLSQRDSGVAAAKELGLEVVSDNVTYQVDTTDFTPVLTPILRTRPDLLVLSGVSPANAP
CCCCCCCCCHHHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCHH
QLIRSARELGFEGLISTETAQDAGVLAEGAGDLANGFISVGGASTPELASPMMNEFVERY
HHHHHHHHCCCCCCEECCCCCCCCCEECCCCHHHCCEEECCCCCCHHHHHHHHHHHHHHH
TAMFGEYNDESNTKVYALEYILETLKATPEAIEDVAAFQASMDDFSAPNPYMNGDEQLTY
HHHHCCCCCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
VGMTSFGQKRQIAVPLVVNVYQDGAFETMFIARVD
EEECCCCCCCEEEEEEEEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MKRRTLMKATLTAASLIALQATMAVAENPVLKIGFVGVTSGPAAAWGISNQRSMETRAAW
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
INETGGYEIGGVTYDIEIVSFDDQKDPKRAIAGMEKMAQEGIHYVVGPNVDDGAAAVRPV
HCCCCCEEECCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCC
AEANDIMYFPYAFPRELYSAPASNAVLSMIANYQSGPAIYKHLMENEGVKTVAFIAANES
CCCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEECCC
DPLSQRDSGVAAAKELGLEVVSDNVTYQVDTTDFTPVLTPILRTRPDLLVLSGVSPANAP
CCCCCCCCCHHHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCHH
QLIRSARELGFEGLISTETAQDAGVLAEGAGDLANGFISVGGASTPELASPMMNEFVERY
HHHHHHHHCCCCCCEECCCCCCCCCEECCCCHHHCCEEECCCCCCHHHHHHHHHHHHHHH
TAMFGEYNDESNTKVYALEYILETLKATPEAIEDVAAFQASMDDFSAPNPYMNGDEQLTY
HHHHCCCCCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
VGMTSFGQKRQIAVPLVVNVYQDGAFETMFIARVD
EEECCCCCCCEEEEEEEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA