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The map label for this gene is rlmH

Identifier: 110678000

GI number: 110678000

Start: 603197

End: 603667

Strand: Direct

Name: rlmH

Synonym: RD1_0621

Alternate gene names: 110678000

Gene position: 603197-603667 (Clockwise)

Preceding gene: 110677999

Following gene: 110678004

Centisome position: 14.59

GC content: 62.63

Gene sequence:

>471_bases
ATGCGTATCCACATCATTGCGGTCGGGCGCTTGCGTGCCGGGCCTGAGAAAGATCTAATCGGCGATTACCTGACGCGTTT
TGCGCGGTCGGGTCGCGCGCTTGGCCTGGGTCCCGCACGCGTTGTCGAAGTCGAGGACCGCAAGAACGGCGGTATGGGCA
ACGAAGCGGCGCTGCTGCGCCGTGCAATTCCAGATGGCGCGCTGATCGCGGTTCTTGATGAACGCGGGCGCGTCGAAAGC
TCACCGGATTTTGCACAAAGACTGGCAGGCTGGCGGGATCAGGGCCGGGGTGATCTGGCCTTAGTGATCGGCGGGGCGGA
TGGGATCGACCCCGCATTGCGCGCCGAGGCAGATTTCGCGCTGTCATTGGGCAAGATGGTCTGGCCGCATATGCTGGTGC
GCGTGATGCTGGCCGAACAATTGTATCGGGCAGCGTCAATTCTGGCCGGCGCGCCCTATCATCGTAACTGA

Upstream 100 bases:

>100_bases
TGTCTTTCGTCCCGAAGTCCGGGAATTCTACCAGCTTGAAAAGATGTGGCAGCCCATGGGCGGCCCCGAAACGGCGGCAA
ACTGAGCCGACACGGCCTCG

Downstream 100 bases:

>100_bases
CAAGAGAGCCCGCGCTGTGTTTACAGCGCTTGATTTCCGGCCAGCTTTCGCTGTGTCGCCAGCGTACCACGGCCCATCAC
GCGCATGAGTTTGACGGCCT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRIHIIAVGRLRAGPEKDLIGDYLTRFARSGRALGLGPARVVEVEDRKNGGMGNEAALLRRAIPDGALIAVLDERGRVES
SPDFAQRLAGWRDQGRGDLALVIGGADGIDPALRAEADFALSLGKMVWPHMLVRVMLAEQLYRAASILAGAPYHRN

Sequences:

>Translated_156_residues
MRIHIIAVGRLRAGPEKDLIGDYLTRFARSGRALGLGPARVVEVEDRKNGGMGNEAALLRRAIPDGALIAVLDERGRVES
SPDFAQRLAGWRDQGRGDLALVIGGADGIDPALRAEADFALSLGKMVWPHMLVRVMLAEQLYRAASILAGAPYHRN
>Mature_156_residues
MRIHIIAVGRLRAGPEKDLIGDYLTRFARSGRALGLGPARVVEVEDRKNGGMGNEAALLRRAIPDGALIAVLDERGRVES
SPDFAQRLAGWRDQGRGDLALVIGGADGIDPALRAEADFALSLGKMVWPHMLVRVMLAEQLYRAASILAGAPYHRN

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=162, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ROSDO (Q16CH2)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681007.1
- ProteinModelPortal:   Q16CH2
- SMR:   Q16CH2
- STRING:   Q16CH2
- GeneID:   4198385
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_0621
- NMPDR:   fig|375451.6.peg.584
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- PhylomeDB:   Q16CH2
- ProtClustDB:   PRK00103
- BioCyc:   RDEN375451:RD1_0621-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16862; Mature: 16862

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHIIAVGRLRAGPEKDLIGDYLTRFARSGRALGLGPARVVEVEDRKNGGMGNEAALLR
CEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCHHHHHHH
RAIPDGALIAVLDERGRVESSPDFAQRLAGWRDQGRGDLALVIGGADGIDPALRAEADFA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHCCCHHH
LSLGKMVWPHMLVRVMLAEQLYRAASILAGAPYHRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRIHIIAVGRLRAGPEKDLIGDYLTRFARSGRALGLGPARVVEVEDRKNGGMGNEAALLR
CEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCHHHHHHH
RAIPDGALIAVLDERGRVESSPDFAQRLAGWRDQGRGDLALVIGGADGIDPALRAEADFA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHCCCHHH
LSLGKMVWPHMLVRVMLAEQLYRAASILAGAPYHRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA