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The map label for this gene is glcD [H]

Identifier: 110678004

GI number: 110678004

Start: 605531

End: 606985

Strand: Direct

Name: glcD [H]

Synonym: RD1_0625

Alternate gene names: 110678004

Gene position: 605531-606985 (Clockwise)

Preceding gene: 110678000

Following gene: 110678005

Centisome position: 14.65

GC content: 60.0

Gene sequence:

>1455_bases
ATGTCGATGGAGATGCCTGTACCTGATGCCGCGATTCTGGCCCGCAAGGCGCGGCTGGTGGAGCGTCTGCAATCCGTGGT
TCCAGCCGATGCGGTCATCCATGATCCGCACCAAACACGCGCTTACGAGTGTGACGCACTGACGGCGTATAAATGCCCGC
CGATGGCTGTTGTGTTGCCGGCGTCCACCAAAGAGGTGTCGGATGTGCTGCGCATTTGTCACGAGGATGGCGTGCCCGTC
GTGCCGCGCGGCTCTGGTACGTCTCTGGCCGGGGGGGCTTTGCCCACGGCGGATTGCGTGATCCTTGGCGTGGCGCGCAT
GAATGACGTGCTGGAAACGAATTATGACGACCGCTACATCCGCGTGGAATCGGGGCGCACGAACCTCAGCGTGACCGGCG
CCGTGGAAGAAGACGGGTTCTTTTACGCGCCCGACCCTTCCAGCCAGTTGGCCTGTGCCATAGCGGGCAACATCGCGATG
AATTCAGGCGGTGCGCATTGCCTGAAATATGGCGTGACCACCAACAACCTGATGGGCGTGACGCTCGTGATGATGGATGG
CGAAATCGTCGAGGTGGGCGGCGCGCATATGGATTCGGCGGGTCTCGATCTGCTCGGTCTGATCTGTGGTTCCGAAGGGC
AGTTGGGCGTCGTGACCGAAGCCACCCTGCGGATTTTGCCCAAACCCGAAGGCGCGCGACCCGTCCTTATGGGGTTTGAC
GACAGTGAGGTTGCGGGCGCCTGTGTCAGCGACATCATCAAGGCGGGCGTCCTGCCGGTTGCGATTGAATTCATGGACCG
GCTGTGCATCGAGACGAGCGAAAATTTCGCACAGGCAGGCTATCCGATGTGCGAGGCGTTGCTGATCGTCGAGGTCGAAG
GATCAGAGGCCGAGATCGACCATCAACTGGACCTCATCATGGAGATCGCGCGCCGCCATGACCCGGTCGAATTGCGCCAA
AGCACCTCTGCGGAGGAAAGCGCGTTGATCTGGCTGGGGCGGAAATCCGCCTTTGGGGCCATCGGGCAAATCAACGATTA
CATGTGTCTGGATGGGACGATCCCGGTATCTTCGCTGCCTTTCGTCTTGCGCCGCATTGGCGAGATGTCCAAAGAGTTCG
GCCTGCAGGTCGGCAATGTGTTTCATGCGGGGGACGGCAACATGCACCCGTTGATCCTGTTTGATGCCAACAAGCCGGGT
GATCTGGAACGCTGCGAAGCGCTTGGCGCGGAAATCCTCAAGCTCTGTGTCGAAGCGGGCGGATGCCTGACCGGAGAACA
CGGTGTCGGGATCGAAAAGCGTGACCTGATGGAATACCAATTCGCGCCCGACGATCTGGAGGCGCAGATGGCCATCAAGG
ACGTGTTCGATGCCCAATGGCTGTTGAACCCGGCCAAAGTCTTTCCGCTTTCCGTTTCACAAAACCGTCGGATGCTTGCC
ATTGCGGCGCAGTAA

Upstream 100 bases:

>100_bases
CAGGGCAAAACGATCCATGATATCCATAGGCAGACGCATGGCAGGTAAGATAATTCTTGCCAATGGTCAAAATTGGTCAT
ATTAATTTACCAAAAGGAGG

Downstream 100 bases:

>100_bases
GGCCCATGCGGGCGTTGCGTTTGGTTCGAAACTCATCACAGGCCATGAATCATGATCACCCCTGACACCGAAGCGGCCCT
GGCCGACGTCATAAAATCGG

Product: glycolate oxidase, subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSMEMPVPDAAILARKARLVERLQSVVPADAVIHDPHQTRAYECDALTAYKCPPMAVVLPASTKEVSDVLRICHEDGVPV
VPRGSGTSLAGGALPTADCVILGVARMNDVLETNYDDRYIRVESGRTNLSVTGAVEEDGFFYAPDPSSQLACAIAGNIAM
NSGGAHCLKYGVTTNNLMGVTLVMMDGEIVEVGGAHMDSAGLDLLGLICGSEGQLGVVTEATLRILPKPEGARPVLMGFD
DSEVAGACVSDIIKAGVLPVAIEFMDRLCIETSENFAQAGYPMCEALLIVEVEGSEAEIDHQLDLIMEIARRHDPVELRQ
STSAEESALIWLGRKSAFGAIGQINDYMCLDGTIPVSSLPFVLRRIGEMSKEFGLQVGNVFHAGDGNMHPLILFDANKPG
DLERCEALGAEILKLCVEAGGCLTGEHGVGIEKRDLMEYQFAPDDLEAQMAIKDVFDAQWLLNPAKVFPLSVSQNRRMLA
IAAQ

Sequences:

>Translated_484_residues
MSMEMPVPDAAILARKARLVERLQSVVPADAVIHDPHQTRAYECDALTAYKCPPMAVVLPASTKEVSDVLRICHEDGVPV
VPRGSGTSLAGGALPTADCVILGVARMNDVLETNYDDRYIRVESGRTNLSVTGAVEEDGFFYAPDPSSQLACAIAGNIAM
NSGGAHCLKYGVTTNNLMGVTLVMMDGEIVEVGGAHMDSAGLDLLGLICGSEGQLGVVTEATLRILPKPEGARPVLMGFD
DSEVAGACVSDIIKAGVLPVAIEFMDRLCIETSENFAQAGYPMCEALLIVEVEGSEAEIDHQLDLIMEIARRHDPVELRQ
STSAEESALIWLGRKSAFGAIGQINDYMCLDGTIPVSSLPFVLRRIGEMSKEFGLQVGNVFHAGDGNMHPLILFDANKPG
DLERCEALGAEILKLCVEAGGCLTGEHGVGIEKRDLMEYQFAPDDLEAQMAIKDVFDAQWLLNPAKVFPLSVSQNRRMLA
IAAQ
>Mature_483_residues
SMEMPVPDAAILARKARLVERLQSVVPADAVIHDPHQTRAYECDALTAYKCPPMAVVLPASTKEVSDVLRICHEDGVPVV
PRGSGTSLAGGALPTADCVILGVARMNDVLETNYDDRYIRVESGRTNLSVTGAVEEDGFFYAPDPSSQLACAIAGNIAMN
SGGAHCLKYGVTTNNLMGVTLVMMDGEIVEVGGAHMDSAGLDLLGLICGSEGQLGVVTEATLRILPKPEGARPVLMGFDD
SEVAGACVSDIIKAGVLPVAIEFMDRLCIETSENFAQAGYPMCEALLIVEVEGSEAEIDHQLDLIMEIARRHDPVELRQS
TSAEESALIWLGRKSAFGAIGQINDYMCLDGTIPVSSLPFVLRRIGEMSKEFGLQVGNVFHAGDGNMHPLILFDANKPGD
LERCEALGAEILKLCVEAGGCLTGEHGVGIEKRDLMEYQFAPDDLEAQMAIKDVFDAQWLLNPAKVFPLSVSQNRRMLAI
AAQ

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=429, Percent_Identity=28.9044289044289, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI37595754, Length=452, Percent_Identity=27.4336283185841, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI119964728, Length=457, Percent_Identity=28.6652078774617, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4501993, Length=245, Percent_Identity=26.1224489795918, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789351, Length=449, Percent_Identity=47.2160356347439, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI48994907, Length=487, Percent_Identity=23.8193018480493, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787977, Length=147, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=461, Percent_Identity=26.0303687635575, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI71992381, Length=461, Percent_Identity=25.5965292841649, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71992392, Length=366, Percent_Identity=25.1366120218579, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17534361, Length=468, Percent_Identity=25.4273504273504, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17556096, Length=218, Percent_Identity=25.2293577981651, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320027, Length=430, Percent_Identity=29.7674418604651, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320764, Length=471, Percent_Identity=24.416135881104, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320023, Length=457, Percent_Identity=25.601750547046, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18921117, Length=434, Percent_Identity=26.9585253456221, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24639277, Length=434, Percent_Identity=26.9585253456221, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24639275, Length=434, Percent_Identity=26.9585253456221, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24653753, Length=217, Percent_Identity=26.7281105990783, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51816; Mature: 51685

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMEMPVPDAAILARKARLVERLQSVVPADAVIHDPHQTRAYECDALTAYKCPPMAVVLP
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCCEEEECCCCEEEEEC
ASTKEVSDVLRICHEDGVPVVPRGSGTSLAGGALPTADCVILGVARMNDVLETNYDDRYI
CCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCEE
RVESGRTNLSVTGAVEEDGFFYAPDPSSQLACAIAGNIAMNSGGAHCLKYGVTTNNLMGV
EEECCCCEEEEEEEEECCCEEECCCCCCCEEEEEECCEEECCCCCEEEEECCCCCCCEEE
TLVMMDGEIVEVGGAHMDSAGLDLLGLICGSEGQLGVVTEATLRILPKPEGARPVLMGFD
EEEEECCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEEECCCCCCCEEEEECC
DSEVAGACVSDIIKAGVLPVAIEFMDRLCIETSENFAQAGYPMCEALLIVEVEGSEAEID
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEEEECCCCCCHH
HQLDLIMEIARRHDPVELRQSTSAEESALIWLGRKSAFGAIGQINDYMCLDGTIPVSSLP
HHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHH
FVLRRIGEMSKEFGLQVGNVFHAGDGNMHPLILFDANKPGDLERCEALGAEILKLCVEAG
HHHHHHHHHHHHHCCEECCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
GCLTGEHGVGIEKRDLMEYQFAPDDLEAQMAIKDVFDAQWLLNPAKVFPLSVSQNRRMLA
CEEECCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCEEEE
IAAQ
EECC
>Mature Secondary Structure 
SMEMPVPDAAILARKARLVERLQSVVPADAVIHDPHQTRAYECDALTAYKCPPMAVVLP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCCEEEECCCCEEEEEC
ASTKEVSDVLRICHEDGVPVVPRGSGTSLAGGALPTADCVILGVARMNDVLETNYDDRYI
CCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCEE
RVESGRTNLSVTGAVEEDGFFYAPDPSSQLACAIAGNIAMNSGGAHCLKYGVTTNNLMGV
EEECCCCEEEEEEEEECCCEEECCCCCCCEEEEEECCEEECCCCCEEEEECCCCCCCEEE
TLVMMDGEIVEVGGAHMDSAGLDLLGLICGSEGQLGVVTEATLRILPKPEGARPVLMGFD
EEEEECCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEEECCCCCCCEEEEECC
DSEVAGACVSDIIKAGVLPVAIEFMDRLCIETSENFAQAGYPMCEALLIVEVEGSEAEID
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEEEECCCCCCHH
HQLDLIMEIARRHDPVELRQSTSAEESALIWLGRKSAFGAIGQINDYMCLDGTIPVSSLP
HHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHH
FVLRRIGEMSKEFGLQVGNVFHAGDGNMHPLILFDANKPGDLERCEALGAEILKLCVEAG
HHHHHHHHHHHHHCCEECCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
GCLTGEHGVGIEKRDLMEYQFAPDDLEAQMAIKDVFDAQWLLNPAKVFPLSVSQNRRMLA
CEEECCCCCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCEEEE
IAAQ
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]