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The map label for this gene is thiB [H]

Identifier: 110677941

GI number: 110677941

Start: 540540

End: 541520

Strand: Direct

Name: thiB [H]

Synonym: RD1_0557

Alternate gene names: 110677941

Gene position: 540540-541520 (Clockwise)

Preceding gene: 110677938

Following gene: 110677942

Centisome position: 13.08

GC content: 58.92

Gene sequence:

>981_bases
ATGCGAAGAACTCTGATAACTGCTGGCATCGTGTTGGCCTCGCAGGCTGCGGCTGAAACACCTGTGCTTACCGTCTACGC
GGGCGATTACTTTACCTCCGAATGGGGGCCCGGCCCGATGATCGAAACCGGGTTTGAAGAGATTTGCGGGTGTGATCTGC
AGTTTTCGACCGGTGATCTTGTGCCGCGGTTGCTGTTGGAAGGCGCGCGCACCAAGGCTGATGTCGTGATGGGTCTGACG
TCGGATGTGACATCCAAAGCACGCGCGACCGGCTTGTTTGCACCGCATGGGCAAGACAACGGGGCGCTGACACTGCCGGT
TGATTGGACGGATGACACCTTCTTGCCATTCAACTACGGGCATGCGGCCTTCGTTTACAATACAGCGACAGTTGCCAACC
CGCCCGCGAGTTTCGAGGCCATGCTGGACATGCCCGACGATGTAAAAATCGTCATTCAGGACCCGCGCACCTCCTTGTCG
GGTCTGGCGCTGGTGCTTTGGGTGCAATCGGTCTTCGGTGATGATGCAGGCGCGGCCTGGGAAAAGCTGGCGCCGAACAT
TCTGACGGTGACCAAGGACTGGTCGGCCAGCTACGGCATGTTCACCGATGGCGAAGCAGACATGGTGCTGAGTTACACGA
CCTCACCCGCCTACCACATGTTTGCCGAGGATGATTTCACCAGAAAGGCCGCGATTTTCCCCGAGGGTCACTATTTCATG
GTCGAAACCGTCGGCAAGATCGCAGCGACGGATCAGCCCGAACTGGCCGATGCCTTCATGGCCTATGTGATGAGCGAGAG
CTTCCAATCCTCGATCCCGCAGGCCAACTGGTCGTTGCCATCTGCGCTGCCGCAGGATCAATGGCCCGAAGGCTGGGCGC
AGCTGCCGCTGCCGGACAAGGTGTTGTTTTACTCCGAAGCGGACGCAACCGCGCTTCAGGCAGAAGCCATCGAGACATGG
CGCGCAGCGCTCAGCCAATAA

Upstream 100 bases:

>100_bases
GCGCGCGGACCCGTAGAACCTGAACCGGTTAGAACCGGCGGAGGGAAGGTTGAGCATATCATCCTCTTTCGCCGTTCGAC
GCATTTGAAAAGGATAGATC

Downstream 100 bases:

>100_bases
GCGGACACGCGGGGATCGTCGCGGGCACTTTGGTGGTTGCGGCGGTCCTGCTGGCGTTTCTTGGCATCGGTTTGCGGGCC
GAGGCGAGCGCGGGCTTCGG

Product: thiamin ABC transporter, periplasmic thiamin-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRRTLITAGIVLASQAAAETPVLTVYAGDYFTSEWGPGPMIETGFEEICGCDLQFSTGDLVPRLLLEGARTKADVVMGLT
SDVTSKARATGLFAPHGQDNGALTLPVDWTDDTFLPFNYGHAAFVYNTATVANPPASFEAMLDMPDDVKIVIQDPRTSLS
GLALVLWVQSVFGDDAGAAWEKLAPNILTVTKDWSASYGMFTDGEADMVLSYTTSPAYHMFAEDDFTRKAAIFPEGHYFM
VETVGKIAATDQPELADAFMAYVMSESFQSSIPQANWSLPSALPQDQWPEGWAQLPLPDKVLFYSEADATALQAEAIETW
RAALSQ

Sequences:

>Translated_326_residues
MRRTLITAGIVLASQAAAETPVLTVYAGDYFTSEWGPGPMIETGFEEICGCDLQFSTGDLVPRLLLEGARTKADVVMGLT
SDVTSKARATGLFAPHGQDNGALTLPVDWTDDTFLPFNYGHAAFVYNTATVANPPASFEAMLDMPDDVKIVIQDPRTSLS
GLALVLWVQSVFGDDAGAAWEKLAPNILTVTKDWSASYGMFTDGEADMVLSYTTSPAYHMFAEDDFTRKAAIFPEGHYFM
VETVGKIAATDQPELADAFMAYVMSESFQSSIPQANWSLPSALPQDQWPEGWAQLPLPDKVLFYSEADATALQAEAIETW
RAALSQ
>Mature_326_residues
MRRTLITAGIVLASQAAAETPVLTVYAGDYFTSEWGPGPMIETGFEEICGCDLQFSTGDLVPRLLLEGARTKADVVMGLT
SDVTSKARATGLFAPHGQDNGALTLPVDWTDDTFLPFNYGHAAFVYNTATVANPPASFEAMLDMPDDVKIVIQDPRTSLS
GLALVLWVQSVFGDDAGAAWEKLAPNILTVTKDWSASYGMFTDGEADMVLSYTTSPAYHMFAEDDFTRKAAIFPEGHYFM
VETVGKIAATDQPELADAFMAYVMSESFQSSIPQANWSLPSALPQDQWPEGWAQLPLPDKVLFYSEADATALQAEAIETW
RAALSQ

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=310, Percent_Identity=44.5161290322581, Blast_Score=282, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35384; Mature: 35384

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTLITAGIVLASQAAAETPVLTVYAGDYFTSEWGPGPMIETGFEEICGCDLQFSTGDL
CCCEEEEHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCCHHHCHHHHCCCCEEECCHHH
VPRLLLEGARTKADVVMGLTSDVTSKARATGLFAPHGQDNGALTLPVDWTDDTFLPFNYG
HHHHHHHCCCCCEEEEEECHHHHHHHHHHCEEECCCCCCCCEEEEEECCCCCCEEEECCC
HAAFVYNTATVANPPASFEAMLDMPDDVKIVIQDPRTSLSGLALVLWVQSVFGDDAGAAW
CEEEEEECCCCCCCCCCHHHEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHH
EKLAPNILTVTKDWSASYGMFTDGEADMVLSYTTSPAYHMFAEDDFTRKAAIFPEGHYFM
HHHCCCEEEEECCCCCCCCEEECCCCCEEEEEECCCCEEEECCCCCCCEEEECCCCCEEE
VETVGKIAATDQPELADAFMAYVMSESFQSSIPQANWSLPSALPQDQWPEGWAQLPLPDK
EEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VLFYSEADATALQAEAIETWRAALSQ
EEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRTLITAGIVLASQAAAETPVLTVYAGDYFTSEWGPGPMIETGFEEICGCDLQFSTGDL
CCCEEEEHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCCHHHCHHHHCCCCEEECCHHH
VPRLLLEGARTKADVVMGLTSDVTSKARATGLFAPHGQDNGALTLPVDWTDDTFLPFNYG
HHHHHHHCCCCCEEEEEECHHHHHHHHHHCEEECCCCCCCCEEEEEECCCCCCEEEECCC
HAAFVYNTATVANPPASFEAMLDMPDDVKIVIQDPRTSLSGLALVLWVQSVFGDDAGAAW
CEEEEEECCCCCCCCCCHHHEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHH
EKLAPNILTVTKDWSASYGMFTDGEADMVLSYTTSPAYHMFAEDDFTRKAAIFPEGHYFM
HHHCCCEEEEECCCCCCCCEEECCCCCEEEEEECCCCEEEECCCCCCCEEEECCCCCEEE
VETVGKIAATDQPELADAFMAYVMSESFQSSIPQANWSLPSALPQDQWPEGWAQLPLPDK
EEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VLFYSEADATALQAEAIETWRAALSQ
EEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]