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The map label for this gene is thiP [H]

Identifier: 110677942

GI number: 110677942

Start: 541496

End: 543052

Strand: Direct

Name: thiP [H]

Synonym: RD1_0558

Alternate gene names: 110677942

Gene position: 541496-543052 (Clockwise)

Preceding gene: 110677941

Following gene: 110677945

Centisome position: 13.1

GC content: 63.13

Gene sequence:

>1557_bases
ATGGCGCGCAGCGCTCAGCCAATAAGCGGACACGCGGGGATCGTCGCGGGCACTTTGGTGGTTGCGGCGGTCCTGCTGGC
GTTTCTTGGCATCGGTTTGCGGGCCGAGGCGAGCGCGGGCTTCGGGGCGGCGGAATGGCAAGCCATCCGCTTCACCGTTG
TGCAGTCCGTCCTGTCCGCGCTGATATCCGTGGCGCTGGCCATTCCGCTGGCTCGTGCGCTGGCGCGGCGTGACCTGCCG
GGACGTCAGTTGCTGATCACCTTGCTGGGTGCGCCCTTTATCCTGCCGGTCATCGTGGCGGTTCTGGGTCTGCTGGCAGT
CTTCGGTCGCAATGGGTGGGTGAATGCCGCCTTTGGCACCTTGGGTCTGCCGCCGGTGTCGATCTATGGCTTGCACGGAG
TGGTATTGGCCCATGTGTTCTTCAACCTGCCGCTGGCCACACGCCTTTTGCTGCAAGGCTGGCAGAGCATCCCGGCAGAG
CGGTTTCGCCTTGCCGCGCAACTGAACCTGTCGCCATGGGTGATCTTTCGCACCATTGAGGTGCCGCTGTTGGCCCGTAT
TCTGCCCGGTGCGGCTGCCTTGATCTTTGTGATTTGTCTGACGAGCTTTGCGGTTGCTCTGACACTGGGGGGCGGGCCGC
GCGCGACGACGATCGAACTGGCTATCTATCAGGCGTTCCGTTTTGACTTTGATCTGTCAAAGGCGGCTTTGCTGTCGGTG
ATCCAGCTTGTTCTGGCGGGCGGCGCTGCCGTGCTGGCGCTTTGGATCATCCCGCAGATATCGCTGACCGGCGGGATGGA
CCGACCCGTGCAACGATGGGACGGGCGCGGCGGTCTGCAACGGTTCGGGGACGGCGTCGCGATCCTGCTCGGTGCCGGGT
TTTTGTTGCTGCCGCTTTTCTCGGTGGCGGTGTCAGGGCTGATCGGCCTGCCAGAGGTGCCCGCCTCGGTATGGCGCGCG
GCGCTGACATCGCTTTGGGTGGCTGGTATCAGCGTATGCGTTCTGTTGGCCGTTGCGCTGCCACTTGCCGGCTGGATCGC
CATCCATGCGCGTGGCAGTGTTGAGGCCATCGGCCTTTTGGGTCTGTCTGCATCACCATTGATGATCGGCACCGGGTGGT
TCATCCTGATCAACCCGATTGCCGATCCTGTCAGTCTGTCGCTTTTGGTAACCGCAGTGGTCAATGCACTGATGGCGCTG
CCCTTTGCGCTGCGCATCGTGGTGCCGGGGATTCGTGAGGCGCTGCAGTCGCATGGGCGGTTGTGCATGACCCTGAACAT
GACCGGGTGGACAACGTGGCGCTGGGTGATCCTGCCGCGAGCGCTGCCGCAGATCGGGTTTGCCGCTGGGCTGACGGGCG
CACTGTCGATTGGGGACCTTGGCGTCATCGCATTGTTTGCGGACCCGGATCGCACAACGCTGCCCTTGCAGATGTATCGC
TTGATGGGGGCCTATAAGACCGAAGCGGCGGCGGGTGCTGCGCTGATATTGCTGGCCATGGCGCTCGGTATTTTCTGGAG
CTTTGACAGAGGGGCGCGCTGGTATGCTCGTACTTGA

Upstream 100 bases:

>100_bases
ATCAATGGCCCGAAGGCTGGGCGCAGCTGCCGCTGCCGGACAAGGTGTTGTTTTACTCCGAAGCGGACGCAACCGCGCTT
CAGGCAGAAGCCATCGAGAC

Downstream 100 bases:

>100_bases
CAACGTATCAATCGCCCAAGGCAGCTTCGCGCTGGACGCGGATTTCACGGTGCAACAGGGGCGCAAAGTTGCGGTGATCG
GGCCCTCTGGTGCGGGGAAG

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MARSAQPISGHAGIVAGTLVVAAVLLAFLGIGLRAEASAGFGAAEWQAIRFTVVQSVLSALISVALAIPLARALARRDLP
GRQLLITLLGAPFILPVIVAVLGLLAVFGRNGWVNAAFGTLGLPPVSIYGLHGVVLAHVFFNLPLATRLLLQGWQSIPAE
RFRLAAQLNLSPWVIFRTIEVPLLARILPGAAALIFVICLTSFAVALTLGGGPRATTIELAIYQAFRFDFDLSKAALLSV
IQLVLAGGAAVLALWIIPQISLTGGMDRPVQRWDGRGGLQRFGDGVAILLGAGFLLLPLFSVAVSGLIGLPEVPASVWRA
ALTSLWVAGISVCVLLAVALPLAGWIAIHARGSVEAIGLLGLSASPLMIGTGWFILINPIADPVSLSLLVTAVVNALMAL
PFALRIVVPGIREALQSHGRLCMTLNMTGWTTWRWVILPRALPQIGFAAGLTGALSIGDLGVIALFADPDRTTLPLQMYR
LMGAYKTEAAAGAALILLAMALGIFWSFDRGARWYART

Sequences:

>Translated_518_residues
MARSAQPISGHAGIVAGTLVVAAVLLAFLGIGLRAEASAGFGAAEWQAIRFTVVQSVLSALISVALAIPLARALARRDLP
GRQLLITLLGAPFILPVIVAVLGLLAVFGRNGWVNAAFGTLGLPPVSIYGLHGVVLAHVFFNLPLATRLLLQGWQSIPAE
RFRLAAQLNLSPWVIFRTIEVPLLARILPGAAALIFVICLTSFAVALTLGGGPRATTIELAIYQAFRFDFDLSKAALLSV
IQLVLAGGAAVLALWIIPQISLTGGMDRPVQRWDGRGGLQRFGDGVAILLGAGFLLLPLFSVAVSGLIGLPEVPASVWRA
ALTSLWVAGISVCVLLAVALPLAGWIAIHARGSVEAIGLLGLSASPLMIGTGWFILINPIADPVSLSLLVTAVVNALMAL
PFALRIVVPGIREALQSHGRLCMTLNMTGWTTWRWVILPRALPQIGFAAGLTGALSIGDLGVIALFADPDRTTLPLQMYR
LMGAYKTEAAAGAALILLAMALGIFWSFDRGARWYART
>Mature_517_residues
ARSAQPISGHAGIVAGTLVVAAVLLAFLGIGLRAEASAGFGAAEWQAIRFTVVQSVLSALISVALAIPLARALARRDLPG
RQLLITLLGAPFILPVIVAVLGLLAVFGRNGWVNAAFGTLGLPPVSIYGLHGVVLAHVFFNLPLATRLLLQGWQSIPAER
FRLAAQLNLSPWVIFRTIEVPLLARILPGAAALIFVICLTSFAVALTLGGGPRATTIELAIYQAFRFDFDLSKAALLSVI
QLVLAGGAAVLALWIIPQISLTGGMDRPVQRWDGRGGLQRFGDGVAILLGAGFLLLPLFSVAVSGLIGLPEVPASVWRAA
LTSLWVAGISVCVLLAVALPLAGWIAIHARGSVEAIGLLGLSASPLMIGTGWFILINPIADPVSLSLLVTAVVNALMALP
FALRIVVPGIREALQSHGRLCMTLNMTGWTTWRWVILPRALPQIGFAAGLTGALSIGDLGVIALFADPDRTTLPLQMYRL
MGAYKTEAAAGAALILLAMALGIFWSFDRGARWYART

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=468, Percent_Identity=42.7350427350427, Blast_Score=279, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 54462; Mature: 54331

Theoretical pI: Translated: 11.46; Mature: 11.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSAQPISGHAGIVAGTLVVAAVLLAFLGIGLRAEASAGFGAAEWQAIRFTVVQSVLSA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
LISVALAIPLARALARRDLPGRQLLITLLGAPFILPVIVAVLGLLAVFGRNGWVNAAFGT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCCC
LGLPPVSIYGLHGVVLAHVFFNLPLATRLLLQGWQSIPAERFRLAAQLNLSPWVIFRTIE
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEHH
VPLLARILPGAAALIFVICLTSFAVALTLGGGPRATTIELAIYQAFRFDFDLSKAALLSV
HHHHHHHHCHHHHHHHHHHHHHHHHHEEECCCCCCEEEEHHHHHHHHCCCCCHHHHHHHH
IQLVLAGGAAVLALWIIPQISLTGGMDRPVQRWDGRGGLQRFGDGVAILLGAGFLLLPLF
HHHHHHCCHHHHHHHHHHHHEECCCCCCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHH
SVAVSGLIGLPEVPASVWRAALTSLWVAGISVCVLLAVALPLAGWIAIHARGSVEAIGLL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEE
GLSASPLMIGTGWFILINPIADPVSLSLLVTAVVNALMALPFALRIVVPGIREALQSHGR
CCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LCMTLNMTGWTTWRWVILPRALPQIGFAAGLTGALSIGDLGVIALFADPDRTTLPLQMYR
EEEEEECCCCCEEEEEEECHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCHHHHHHH
LMGAYKTEAAAGAALILLAMALGIFWSFDRGARWYART
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ARSAQPISGHAGIVAGTLVVAAVLLAFLGIGLRAEASAGFGAAEWQAIRFTVVQSVLSA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
LISVALAIPLARALARRDLPGRQLLITLLGAPFILPVIVAVLGLLAVFGRNGWVNAAFGT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCCC
LGLPPVSIYGLHGVVLAHVFFNLPLATRLLLQGWQSIPAERFRLAAQLNLSPWVIFRTIE
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEHH
VPLLARILPGAAALIFVICLTSFAVALTLGGGPRATTIELAIYQAFRFDFDLSKAALLSV
HHHHHHHHCHHHHHHHHHHHHHHHHHEEECCCCCCEEEEHHHHHHHHCCCCCHHHHHHHH
IQLVLAGGAAVLALWIIPQISLTGGMDRPVQRWDGRGGLQRFGDGVAILLGAGFLLLPLF
HHHHHHCCHHHHHHHHHHHHEECCCCCCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHH
SVAVSGLIGLPEVPASVWRAALTSLWVAGISVCVLLAVALPLAGWIAIHARGSVEAIGLL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEE
GLSASPLMIGTGWFILINPIADPVSLSLLVTAVVNALMALPFALRIVVPGIREALQSHGR
CCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LCMTLNMTGWTTWRWVILPRALPQIGFAAGLTGALSIGDLGVIALFADPDRTTLPLQMYR
EEEEEECCCCCEEEEEEECHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCHHHHHHH
LMGAYKTEAAAGAALILLAMALGIFWSFDRGARWYART
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA