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The map label for this gene is dkgB [H]

Identifier: 110677897

GI number: 110677897

Start: 496950

End: 497768

Strand: Direct

Name: dkgB [H]

Synonym: RD1_0511

Alternate gene names: 110677897

Gene position: 496950-497768 (Clockwise)

Preceding gene: 110677893

Following gene: 110677901

Centisome position: 12.02

GC content: 52.5

Gene sequence:

>819_bases
ATGAACATCTATTTCAAACATTCGAACCAACGCTCATTTGGCACCTATCCTCTGAAAGGTACTGCTTGCAGAGACGCCAT
TCTCACCGCTGCCGAGGTTGGTTATCGCGCGTTTGATACGGCGCAGATGTATCGAAATGAGGCCGAGACAGGGGCCGCTC
TGAAAGAAACGGGTATTCCGCGTGATACGCTCTGCGTGACGACAAAAGTCGAGAATGACAATTATGACGAAAGTAAATTT
CTTGCTTCTGTTGAGGCCAGCCTGAAAGCCTTGCAGTTGGATTATGTGGATGTTTTGCTGCTGCACTGGCCGCCTGCGGA
TTTCCAGATCGAAGGCTCGCTGACCTTGCTGGAACAAGCGCATAAACGGGGCATGGCCCGTAATATCGGCGTGTCCAACT
ATACAGTCCAGATGATGCGCGATGCGACCAGGATTATTGAAACGCCCCTTGTGACAAATCAGATCGAATTCCACCCGCTT
CTGAACCAAGACGTCATGCTGGCCGCCGCATCCGAAACCGGTATTCCGCTGTCGTCTTATTGTTCGGTTGCGCGTGGTGA
GGTGTTCAAACATCCGATCTTTGCCGAGATTGGCGCCGGCTATGGCAAAACCGCGGCACAGGTCGTGCTGCGCTGGATCC
TGCAAAAAGGTGTCAGCATCAACACCATGTCCACCAAACCCGAGAATATCGCGGCGAACTTCGATGTGATGGATTTCACG
CTGTCCTCGGTTGACATGGCCCGGATCGAGCGGATGACGCATATGAATTACCGTATCGTCAGCAAGGATATCGTGCCATG
GGCACCCGATTTCGACTGA

Upstream 100 bases:

>100_bases
AAAAGACTATGCCCACAATCCAGCATGCGATCAAGACTCTTTGATGCTTCCCATTAAGTAACTAAAGGGTTACAATCAGA
GCTATCAGGAAAGGCGCGAC

Downstream 100 bases:

>100_bases
CCCGGATGCTCTGCCGATGTCAGTGGTTTCCTCGGATCATATCTGCGGCTTTCTCGCCGATCATGATCGTCGGGGCATTG
GTGTTTGACCCGATCAGAGA

Product: 2,5-diketo-D-gluconic acid reductase B, putative

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNIYFKHSNQRSFGTYPLKGTACRDAILTAAEVGYRAFDTAQMYRNEAETGAALKETGIPRDTLCVTTKVENDNYDESKF
LASVEASLKALQLDYVDVLLLHWPPADFQIEGSLTLLEQAHKRGMARNIGVSNYTVQMMRDATRIIETPLVTNQIEFHPL
LNQDVMLAAASETGIPLSSYCSVARGEVFKHPIFAEIGAGYGKTAAQVVLRWILQKGVSINTMSTKPENIAANFDVMDFT
LSSVDMARIERMTHMNYRIVSKDIVPWAPDFD

Sequences:

>Translated_272_residues
MNIYFKHSNQRSFGTYPLKGTACRDAILTAAEVGYRAFDTAQMYRNEAETGAALKETGIPRDTLCVTTKVENDNYDESKF
LASVEASLKALQLDYVDVLLLHWPPADFQIEGSLTLLEQAHKRGMARNIGVSNYTVQMMRDATRIIETPLVTNQIEFHPL
LNQDVMLAAASETGIPLSSYCSVARGEVFKHPIFAEIGAGYGKTAAQVVLRWILQKGVSINTMSTKPENIAANFDVMDFT
LSSVDMARIERMTHMNYRIVSKDIVPWAPDFD
>Mature_272_residues
MNIYFKHSNQRSFGTYPLKGTACRDAILTAAEVGYRAFDTAQMYRNEAETGAALKETGIPRDTLCVTTKVENDNYDESKF
LASVEASLKALQLDYVDVLLLHWPPADFQIEGSLTLLEQAHKRGMARNIGVSNYTVQMMRDATRIIETPLVTNQIEFHPL
LNQDVMLAAASETGIPLSSYCSVARGEVFKHPIFAEIGAGYGKTAAQVVLRWILQKGVSINTMSTKPENIAANFDVMDFT
LSSVDMARIERMTHMNYRIVSKDIVPWAPDFD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=26.7123287671233, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=26.7123287671233, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI223468663, Length=273, Percent_Identity=29.6703296703297, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=28.9285714285714, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI24497585, Length=299, Percent_Identity=28.4280936454849, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=29.277566539924, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI93277124, Length=276, Percent_Identity=27.536231884058, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI310109920, Length=299, Percent_Identity=27.4247491638796, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI45446745, Length=299, Percent_Identity=27.0903010033445, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4503285, Length=299, Percent_Identity=27.0903010033445, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=27.4247491638796, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI5174695, Length=276, Percent_Identity=27.536231884058, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI4502049, Length=285, Percent_Identity=28.7719298245614, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI24497583, Length=278, Percent_Identity=26.978417266187, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI300116273, Length=252, Percent_Identity=26.984126984127, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786400, Length=263, Percent_Identity=38.0228136882129, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI87082198, Length=251, Percent_Identity=37.0517928286853, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1788081, Length=239, Percent_Identity=28.4518828451883, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787674, Length=273, Percent_Identity=25.2747252747253, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17561298, Length=253, Percent_Identity=32.8063241106719, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17537077, Length=286, Percent_Identity=31.8181818181818, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17537075, Length=286, Percent_Identity=31.4685314685315, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71998625, Length=246, Percent_Identity=30.4878048780488, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17552492, Length=259, Percent_Identity=27.7992277992278, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538386, Length=257, Percent_Identity=30.3501945525292, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17564128, Length=273, Percent_Identity=28.9377289377289, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17550248, Length=290, Percent_Identity=28.9655172413793, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17537079, Length=290, Percent_Identity=23.7931034482759, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17566692, Length=272, Percent_Identity=27.2058823529412, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17561300, Length=239, Percent_Identity=28.0334728033473, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562292, Length=231, Percent_Identity=31.1688311688312, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324694, Length=288, Percent_Identity=30.9027777777778, Blast_Score=136, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320576, Length=284, Percent_Identity=29.9295774647887, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320079, Length=232, Percent_Identity=31.4655172413793, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321896, Length=305, Percent_Identity=25.2459016393443, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322556, Length=251, Percent_Identity=25.8964143426295, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319625, Length=262, Percent_Identity=27.8625954198473, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356425, Length=303, Percent_Identity=31.3531353135314, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24657054, Length=302, Percent_Identity=30.794701986755, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24663317, Length=286, Percent_Identity=29.020979020979, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129731, Length=258, Percent_Identity=32.1705426356589, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24662789, Length=277, Percent_Identity=27.4368231046931, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24644950, Length=289, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI221378297, Length=308, Percent_Identity=27.9220779220779, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45553081, Length=271, Percent_Identity=25.830258302583, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24662785, Length=266, Percent_Identity=27.4436090225564, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24662781, Length=266, Percent_Identity=27.4436090225564, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281366140, Length=250, Percent_Identity=24.4, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 30369; Mature: 30369

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYFKHSNQRSFGTYPLKGTACRDAILTAAEVGYRAFDTAQMYRNEAETGAALKETGIP
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCC
RDTLCVTTKVENDNYDESKFLASVEASLKALQLDYVDVLLLHWPPADFQIEGSLTLLEQA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEECCCHHHHHHH
HKRGMARNIGVSNYTVQMMRDATRIIETPLVTNQIEFHPLLNQDVMLAAASETGIPLSSY
HHCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHH
CSVARGEVFKHPIFAEIGAGYGKTAAQVVLRWILQKGVSINTMSTKPENIAANFDVMDFT
HHHHHCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCEEEEEE
LSSVDMARIERMTHMNYRIVSKDIVPWAPDFD
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNIYFKHSNQRSFGTYPLKGTACRDAILTAAEVGYRAFDTAQMYRNEAETGAALKETGIP
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCC
RDTLCVTTKVENDNYDESKFLASVEASLKALQLDYVDVLLLHWPPADFQIEGSLTLLEQA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEECCCHHHHHHH
HKRGMARNIGVSNYTVQMMRDATRIIETPLVTNQIEFHPLLNQDVMLAAASETGIPLSSY
HHCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHH
CSVARGEVFKHPIFAEIGAGYGKTAAQVVLRWILQKGVSINTMSTKPENIAANFDVMDFT
HHHHHCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCEEEEEE
LSSVDMARIERMTHMNYRIVSKDIVPWAPDFD
HHHHHHHHHHHHHHCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]