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The map label for this gene is hemN [C]

Identifier: 110677822

GI number: 110677822

Start: 418506

End: 419663

Strand: Direct

Name: hemN [C]

Synonym: RD1_0428

Alternate gene names: 110677822

Gene position: 418506-419663 (Clockwise)

Preceding gene: 110677821

Following gene: 110677830

Centisome position: 10.13

GC content: 57.34

Gene sequence:

>1158_bases
TTGGCTGAAGACTGGGAACACGGCGGCTTTGGCCTCTATATCCACTGGCCCTATTGCGAGGCCAAATGTCCGTATTGTGA
TTTCAACAGCCATGTGGTGCGCCGCATTGATCAAACCGCATGGCGTGACGCCTATCTGACAGAGTTGCGCCGTACAGCGC
GTGAAACGCAAGGCCGCGTGTTGCGATCCGTCTTTTTTGGCGGCGGCACCCCCAGCTTGATGGATCCCGATACGGTTGCG
GTTGTCATCAGCGAAGTGCGCAAGCTTTGGCCGGTTGCCAATGATCTGGAAATCACGCTGGAGGCGAACCCAAGTTCGGT
CGAGGCCGGTCGCTTTGCCGCTTTCCGCCAGGGCGGTGTGACCCGCATTTCCATGGGCATTCAGGCGTTGAACGATGATG
ATCTGCGCCGTCTGGGCCGCATCCACACGGTCACAGAAGCGCAGACCGCCTTCGATATCGCGCGGTCGGAATTTGATCGT
GTCAGCTTTGATTTGATCTATGCGCGGCAGGGCCAGAGCTTGCACGCATGGGAGCAGGAATTGAAACAAGCGCTGGAGAT
GGCGGTCGATCATCTGTCACTCTATCAACTGACCATCGAACAGGGTACGGCGTTCGGCGACCGCTACAACGCGGGTAAAC
TGCGCGGCCTGCCAGATGAAGACCTGAGCGCCGATCTTTACACGCTCACGCAGGACCTATGCGGTGCGGCCGGCATGCCC
GGTTATGAGGTATCGAACCATGCGCGTGACGGGGCACACTCCCGGCATAACCTGATCTATTGGCGCTATGGCGATTACCT
CGGCATCGGACCCGGCGCGCATGGTCGCCTGACCGTGAACGGGCAACGATACGCGACGGAATGCTATTCGAACCCGGCAA
AATGGCTGGCCGCGGTAGGTGATGAGCATACAGAAAAACCGCGTGGGCCGCTTACTTACCAAGATCAGGCGAGCGAATTC
CTGTTGATGGGATTGCGCCTGAAAGAAGGGATAGACACGCACCGTTACGAAACGCTCGCCGGTAAAGCGTTGGACTCCCA
TGCCGTATCAGAGCTGGCAGACCTTGGAATGGTCTCAACCACGGAAGATAGAATAACCGTTTCAGATCAAGGGTTTATCA
TATTAAATGCGGTCATCGCGCGCCTGCTGGAAACCTAG

Upstream 100 bases:

>100_bases
CCTGACGGTCATGACATGACCTTTGCCGAAATGGATCCGGCGGAAAAGAACCGGATCAGCCATCGCGCGGATGCGTTTCA
AAAGCTGATCGCGGGTTGCT

Downstream 100 bases:

>100_bases
GCTCCACTCAAGATCCGGCACAGCTCATCCAGATCGTCGAGTGATTTGTAATGCAGCGTCATGTGCCCGCTTTCGTGACC
GGGTTTGTGGGTCAGTTCGA

Product: coproporphyrinogen III oxidase

Products: Protoporphyrinogen IX; CO2; H2O

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MAEDWEHGGFGLYIHWPYCEAKCPYCDFNSHVVRRIDQTAWRDAYLTELRRTARETQGRVLRSVFFGGGTPSLMDPDTVA
VVISEVRKLWPVANDLEITLEANPSSVEAGRFAAFRQGGVTRISMGIQALNDDDLRRLGRIHTVTEAQTAFDIARSEFDR
VSFDLIYARQGQSLHAWEQELKQALEMAVDHLSLYQLTIEQGTAFGDRYNAGKLRGLPDEDLSADLYTLTQDLCGAAGMP
GYEVSNHARDGAHSRHNLIYWRYGDYLGIGPGAHGRLTVNGQRYATECYSNPAKWLAAVGDEHTEKPRGPLTYQDQASEF
LLMGLRLKEGIDTHRYETLAGKALDSHAVSELADLGMVSTTEDRITVSDQGFIILNAVIARLLET

Sequences:

>Translated_385_residues
MAEDWEHGGFGLYIHWPYCEAKCPYCDFNSHVVRRIDQTAWRDAYLTELRRTARETQGRVLRSVFFGGGTPSLMDPDTVA
VVISEVRKLWPVANDLEITLEANPSSVEAGRFAAFRQGGVTRISMGIQALNDDDLRRLGRIHTVTEAQTAFDIARSEFDR
VSFDLIYARQGQSLHAWEQELKQALEMAVDHLSLYQLTIEQGTAFGDRYNAGKLRGLPDEDLSADLYTLTQDLCGAAGMP
GYEVSNHARDGAHSRHNLIYWRYGDYLGIGPGAHGRLTVNGQRYATECYSNPAKWLAAVGDEHTEKPRGPLTYQDQASEF
LLMGLRLKEGIDTHRYETLAGKALDSHAVSELADLGMVSTTEDRITVSDQGFIILNAVIARLLET
>Mature_384_residues
AEDWEHGGFGLYIHWPYCEAKCPYCDFNSHVVRRIDQTAWRDAYLTELRRTARETQGRVLRSVFFGGGTPSLMDPDTVAV
VISEVRKLWPVANDLEITLEANPSSVEAGRFAAFRQGGVTRISMGIQALNDDDLRRLGRIHTVTEAQTAFDIARSEFDRV
SFDLIYARQGQSLHAWEQELKQALEMAVDHLSLYQLTIEQGTAFGDRYNAGKLRGLPDEDLSADLYTLTQDLCGAAGMPG
YEVSNHARDGAHSRHNLIYWRYGDYLGIGPGAHGRLTVNGQRYATECYSNPAKWLAAVGDEHTEKPRGPLTYQDQASEFL
LMGLRLKEGIDTHRYETLAGKALDSHAVSELADLGMVSTTEDRITVSDQGFIILNAVIARLLET

Specific function: Unknown

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=271, Percent_Identity=45.3874538745387, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI1789325, Length=381, Percent_Identity=36.745406824147, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082341, Length=397, Percent_Identity=25.4408060453401, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: 1.3.3.3

Molecular weight: Translated: 43005; Mature: 42873

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDWEHGGFGLYIHWPYCEAKCPYCDFNSHVVRRIDQTAWRDAYLTELRRTARETQGRV
CCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRSVFFGGGTPSLMDPDTVAVVISEVRKLWPVANDLEITLEANPSSVEAGRFAAFRQGGV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCC
TRISMGIQALNDDDLRRLGRIHTVTEAQTAFDIARSEFDRVSFDLIYARQGQSLHAWEQE
EEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHH
LKQALEMAVDHLSLYQLTIEQGTAFGDRYNAGKLRGLPDEDLSADLYTLTQDLCGAAGMP
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
GYEVSNHARDGAHSRHNLIYWRYGDYLGIGPGAHGRLTVNGQRYATECYSNPAKWLAAVG
CCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCCCEEEECCCHHHHHHHCCHHHHHHHHC
DEHTEKPRGPLTYQDQASEFLLMGLRLKEGIDTHRYETLAGKALDSHAVSELADLGMVST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TEDRITVSDQGFIILNAVIARLLET
CCCEEEECCCCEEHHHHHHHHHHCC
>Mature Secondary Structure 
AEDWEHGGFGLYIHWPYCEAKCPYCDFNSHVVRRIDQTAWRDAYLTELRRTARETQGRV
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRSVFFGGGTPSLMDPDTVAVVISEVRKLWPVANDLEITLEANPSSVEAGRFAAFRQGGV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCC
TRISMGIQALNDDDLRRLGRIHTVTEAQTAFDIARSEFDRVSFDLIYARQGQSLHAWEQE
EEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHH
LKQALEMAVDHLSLYQLTIEQGTAFGDRYNAGKLRGLPDEDLSADLYTLTQDLCGAAGMP
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
GYEVSNHARDGAHSRHNLIYWRYGDYLGIGPGAHGRLTVNGQRYATECYSNPAKWLAAVG
CCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCCCEEEECCCHHHHHHHCCHHHHHHHHC
DEHTEKPRGPLTYQDQASEFLLMGLRLKEGIDTHRYETLAGKALDSHAVSELADLGMVST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TEDRITVSDQGFIILNAVIARLLET
CCCEEEECCCCEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coproporphyrinogen III; O2; H+

Specific reaction: coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]