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The map label for this gene is rph

Identifier: 110677820

GI number: 110677820

Start: 417192

End: 417905

Strand: Direct

Name: rph

Synonym: RD1_0426

Alternate gene names: 110677820

Gene position: 417192-417905 (Clockwise)

Preceding gene: 110677817

Following gene: 110677821

Centisome position: 10.09

GC content: 60.08

Gene sequence:

>714_bases
ATGCGTCCCTCCGGTCGAGAACTGAACGAAATGCGCCCTGTGTCCATCGAAACGGGTTTTACCAAGCATGCCGAAGGCTC
GGCGCTGATCAAGATCGGGGATACTCACGTCTTGTGTACCGCCACGATTGAAGACCGGGTGCCGCCTTTCATCAAAGGGT
CGGGATTGGGTTGGGTCACGGCGGAATATGGCATGCTGCCGCGTGCGACGAACACGCGGATGCGGCGCGAATCCACCGCA
GGCAAGCAAGGCGGGCGTACCGTTGAAATCCAGCGTCTGATCGGGCGCAGCCTGCGCGCTGGCGTTGACCGTGTTGCCCT
CGGCGAGCGACAGATCACCGTCGATTGCGATGTGTTGCAGGCGGATGGCGGCACGCGCTGCGCCTCGATCACGGGCGGTT
GGGTGGCGCTGCGTCTGGCGGTGAACAAGCTGATGAAAGCAGGGGATGTGATCAGTGATCCGCTCGTTGATCCGGTGGCC
GCGATCAGCTGTGGTATCTATGCAGGTCAGCCGGTCATGGATTTGGATTACCCTGAGGATTCCGAGGCGGGTGTTGACGG
GAACTTCATCATGACGGGTTCAAAACAACTGATCGAAGTGCAGATGAGCGCCGAAGGCGCGACGTTTTCACGCGATCAGA
TGAACCAGTTGATGGATCTGGCCGAAAAGGGCGTGGGCGAATTGGTCGCCGCGCAAAAAGCGGCCACCGCATGA

Upstream 100 bases:

>100_bases
ACCCCATATGATAAAAGCATAAACCACAGAGATTTCAATCGGGGTTGCATCCGGGTCGCCCGGCCCTGTATCGACATCAG
GCACATATAAAGGAAATGAA

Downstream 100 bases:

>100_bases
CACGCAAGTTCGACGGCAAACGTATCCTGATTGCGACCCATAACGCGGGCAAGCTGGAAGAAATGCAGCAGCTTTTCGCG
CCCTACGGTGTGGATGTGGT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSGRELNEMRPVSIETGFTKHAEGSALIKIGDTHVLCTATIEDRVPPFIKGSGLGWVTAEYGMLPRATNTRMRRESTA
GKQGGRTVEIQRLIGRSLRAGVDRVALGERQITVDCDVLQADGGTRCASITGGWVALRLAVNKLMKAGDVISDPLVDPVA
AISCGIYAGQPVMDLDYPEDSEAGVDGNFIMTGSKQLIEVQMSAEGATFSRDQMNQLMDLAEKGVGELVAAQKAATA

Sequences:

>Translated_237_residues
MRPSGRELNEMRPVSIETGFTKHAEGSALIKIGDTHVLCTATIEDRVPPFIKGSGLGWVTAEYGMLPRATNTRMRRESTA
GKQGGRTVEIQRLIGRSLRAGVDRVALGERQITVDCDVLQADGGTRCASITGGWVALRLAVNKLMKAGDVISDPLVDPVA
AISCGIYAGQPVMDLDYPEDSEAGVDGNFIMTGSKQLIEVQMSAEGATFSRDQMNQLMDLAEKGVGELVAAQKAATA
>Mature_237_residues
MRPSGRELNEMRPVSIETGFTKHAEGSALIKIGDTHVLCTATIEDRVPPFIKGSGLGWVTAEYGMLPRATNTRMRRESTA
GKQGGRTVEIQRLIGRSLRAGVDRVALGERQITVDCDVLQADGGTRCASITGGWVALRLAVNKLMKAGDVISDPLVDPVA
AISCGIYAGQPVMDLDYPEDSEAGVDGNFIMTGSKQLIEVQMSAEGATFSRDQMNQLMDLAEKGVGELVAAQKAATA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=59.7156398104265, Blast_Score=252, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_ROSDO (Q16D02)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_680827.1
- ProteinModelPortal:   Q16D02
- SMR:   Q16D02
- STRING:   Q16D02
- GeneID:   4197993
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_0426
- NMPDR:   fig|375451.6.peg.402
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q16D02
- ProtClustDB:   PRK00173
- BioCyc:   RDEN375451:RD1_0426-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25237; Mature: 25237

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRELNEMRPVSIETGFTKHAEGSALIKIGDTHVLCTATIEDRVPPFIKGSGLGWVT
CCCCCCCHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEEEHHHCCCCCEECCCCEEEE
AEYGMLPRATNTRMRRESTAGKQGGRTVEIQRLIGRSLRAGVDRVALGERQITVDCDVLQ
ECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCHHEECCCEEEEEEEEEEE
ADGGTRCASITGGWVALRLAVNKLMKAGDVISDPLVDPVAAISCGIYAGQPVMDLDYPED
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCC
SEAGVDGNFIMTGSKQLIEVQMSAEGATFSRDQMNQLMDLAEKGVGELVAAQKAATA
CCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure
MRPSGRELNEMRPVSIETGFTKHAEGSALIKIGDTHVLCTATIEDRVPPFIKGSGLGWVT
CCCCCCCHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEEEEHHHCCCCCEECCCCEEEE
AEYGMLPRATNTRMRRESTAGKQGGRTVEIQRLIGRSLRAGVDRVALGERQITVDCDVLQ
ECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCHHEECCCEEEEEEEEEEE
ADGGTRCASITGGWVALRLAVNKLMKAGDVISDPLVDPVAAISCGIYAGQPVMDLDYPED
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCC
SEAGVDGNFIMTGSKQLIEVQMSAEGATFSRDQMNQLMDLAEKGVGELVAAQKAATA
CCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA