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The map label for this gene is argG [H]

Identifier: 110677812

GI number: 110677812

Start: 406646

End: 407866

Strand: Direct

Name: argG [H]

Synonym: RD1_0417

Alternate gene names: 110677812

Gene position: 406646-407866 (Clockwise)

Preceding gene: 110677809

Following gene: 110677814

Centisome position: 9.84

GC content: 58.07

Gene sequence:

>1221_bases
ATGTCCGCGCCAAAAAAAGTTGTGCTCGCCTATTCAGGTGGTCTTGATACCTCCATCATCCTCAAGTGGCTGCAAACGGA
ATACGGCTGCGAGGTCGTGACCTTCACCGCCGATCTGGGACAGGGTGAAGAGCTTGAACCCGCCCGCGCCAAGGCCGAGA
TGATGGGCGCCTCCGACATCTATATCGAAGACGTGCGCGAAGAATTCGTGCGCGATTTCGTCTTTCCGATGTTCCGCGCC
AATGCGGTTTACGAGGGGCTGTACCTGCTGGGCACGTCGATCGCGCGCCCGCTTATTTCCAAACGTCTTGTTGAAATCGC
CGAAGAGACCGGCGCGGATGCCATCGCCCATGGCGCAACCGGCAAGGGCAACGATCAGGTGCGGTTCGAACTGGCTGCAT
ATGCGCTGAACCCGGACATCAAGGTCATCGCCCCCTGGCGCGAATGGGATCTGTCCAGCCGTACCAAACTGATTGACTTT
GCAGAGAAAAATCAAATTCCAATTGCCAAGGACAAGCGTGGCGAGGCACCGTTTTCCGTCGATGCCAACCTGTTGCACAC
CTCCTCCGAAGGCAAGGTTCTCGAAAACCCGGCTGTGGATGCGCCAGACTATGTCTATCAGCGGACCGTCAACCCCGAGG
ACGCGCCTGACACGCCCGAGTATATCGAGATCGGTTTCGAGAAAGGCGACGCGGTCAGCATCAATGGTGAGGCGATGTCC
CCCGCCACGATCCTGACCAAGCTGAATGAGATCGGCGGCAAACACGGCTGCGGGCGTCTCGACCTTGTTGAGGGGCGTTT
TGTCGGGATGAAATCGCGCGGTATCTACGAAACACCCGGCGGCACGCTTTTGCTGGAGGCGCATCGCGGTATCGAATCGA
TCACGCTGGATCGCGGGGCCATGCATCTCAAGGATGAGTTGATGCCGCGCTATGCTGAGCTGATCTACAACGGTTTCTGG
TTCAGCCCCGAACGCACCATGTTGCAAGCCGCGATTGACGCGAGCCAAACCCATGTGACCGGCACCGTGCGTCTCAAGCT
GTACAAGGGTCTTGCGCGCACCGTTGGCCGCTGGTCTGATCATTCGCTCTATTCCGAGGCCCATGTGACCTTTGAAGAGG
ATGCGGGGGCCTATGATCAAACGGATGCCGCAGGCTTTATCCAGTTGAATGCCCTGCGTTTGAAGCTGCTTGCTGCGCGG
GATCGTCGCCTCAAGAGCTAA

Upstream 100 bases:

>100_bases
TCGATACGCCGCGCCCCCGCTCAGGTCAACGCAGGCTTCATTCTTGCCCGCGCCTTCAAAACCCGGTAGGCCGCGTGGCA
AATCGAAAAGGAAGATCTTC

Downstream 100 bases:

>100_bases
GGCATCATGCGAAAAACGTGGATCACGTAACGCTGCCTGAAATCAAGGATTTCAACGCATTACGTGATACTCATTGTTTC
AGTCGTTTCGTCAGACGCCA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARAKAEMMGASDIYIEDVREEFVRDFVFPMFRA
NAVYEGLYLLGTSIARPLISKRLVEIAEETGADAIAHGATGKGNDQVRFELAAYALNPDIKVIAPWREWDLSSRTKLIDF
AEKNQIPIAKDKRGEAPFSVDANLLHTSSEGKVLENPAVDAPDYVYQRTVNPEDAPDTPEYIEIGFEKGDAVSINGEAMS
PATILTKLNEIGGKHGCGRLDLVEGRFVGMKSRGIYETPGGTLLLEAHRGIESITLDRGAMHLKDELMPRYAELIYNGFW
FSPERTMLQAAIDASQTHVTGTVRLKLYKGLARTVGRWSDHSLYSEAHVTFEEDAGAYDQTDAAGFIQLNALRLKLLAAR
DRRLKS

Sequences:

>Translated_406_residues
MSAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARAKAEMMGASDIYIEDVREEFVRDFVFPMFRA
NAVYEGLYLLGTSIARPLISKRLVEIAEETGADAIAHGATGKGNDQVRFELAAYALNPDIKVIAPWREWDLSSRTKLIDF
AEKNQIPIAKDKRGEAPFSVDANLLHTSSEGKVLENPAVDAPDYVYQRTVNPEDAPDTPEYIEIGFEKGDAVSINGEAMS
PATILTKLNEIGGKHGCGRLDLVEGRFVGMKSRGIYETPGGTLLLEAHRGIESITLDRGAMHLKDELMPRYAELIYNGFW
FSPERTMLQAAIDASQTHVTGTVRLKLYKGLARTVGRWSDHSLYSEAHVTFEEDAGAYDQTDAAGFIQLNALRLKLLAAR
DRRLKS
>Mature_405_residues
SAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARAKAEMMGASDIYIEDVREEFVRDFVFPMFRAN
AVYEGLYLLGTSIARPLISKRLVEIAEETGADAIAHGATGKGNDQVRFELAAYALNPDIKVIAPWREWDLSSRTKLIDFA
EKNQIPIAKDKRGEAPFSVDANLLHTSSEGKVLENPAVDAPDYVYQRTVNPEDAPDTPEYIEIGFEKGDAVSINGEAMSP
ATILTKLNEIGGKHGCGRLDLVEGRFVGMKSRGIYETPGGTLLLEAHRGIESITLDRGAMHLKDELMPRYAELIYNGFWF
SPERTMLQAAIDASQTHVTGTVRLKLYKGLARTVGRWSDHSLYSEAHVTFEEDAGAYDQTDAAGFIQLNALRLKLLAARD
RRLKS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=48.5148514851485, Blast_Score=370, Evalue=1e-103,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=48.5148514851485, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1789563, Length=425, Percent_Identity=28.9411764705882, Blast_Score=133, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=405, Percent_Identity=42.2222222222222, Blast_Score=323, Evalue=2e-89,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=45.2088452088452, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44852; Mature: 44721

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARAKAEMMGASDI
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCH
YIEDVREEFVRDFVFPMFRANAVYEGLYLLGTSIARPLISKRLVEIAEETGADAIAHGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
GKGNDQVRFELAAYALNPDIKVIAPWREWDLSSRTKLIDFAEKNQIPIAKDKRGEAPFSV
CCCCCEEEEEEEEEEECCCEEEEECCHHCCCCCCCEEEEHHCCCCCCCCCCCCCCCCCEE
DANLLHTSSEGKVLENPAVDAPDYVYQRTVNPEDAPDTPEYIEIGFEKGDAVSINGEAMS
CCEEEEECCCCCEECCCCCCCCCHHHEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCC
PATILTKLNEIGGKHGCGRLDLVEGRFVGMKSRGIYETPGGTLLLEAHRGIESITLDRGA
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEECCCCEEEEEECCCCCEEEECCCC
MHLKDELMPRYAELIYNGFWFSPERTMLQAAIDASQTHVTGTVRLKLYKGLARTVGRWSD
HHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHCCCCC
HSLYSEAHVTFEEDAGAYDQTDAAGFIQLNALRLKLLAARDRRLKS
CCCHHCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARAKAEMMGASDI
CCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCH
YIEDVREEFVRDFVFPMFRANAVYEGLYLLGTSIARPLISKRLVEIAEETGADAIAHGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
GKGNDQVRFELAAYALNPDIKVIAPWREWDLSSRTKLIDFAEKNQIPIAKDKRGEAPFSV
CCCCCEEEEEEEEEEECCCEEEEECCHHCCCCCCCEEEEHHCCCCCCCCCCCCCCCCCEE
DANLLHTSSEGKVLENPAVDAPDYVYQRTVNPEDAPDTPEYIEIGFEKGDAVSINGEAMS
CCEEEEECCCCCEECCCCCCCCCHHHEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCC
PATILTKLNEIGGKHGCGRLDLVEGRFVGMKSRGIYETPGGTLLLEAHRGIESITLDRGA
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEECCCCEEEEEECCCCCEEEECCCC
MHLKDELMPRYAELIYNGFWFSPERTMLQAAIDASQTHVTGTVRLKLYKGLARTVGRWSD
HHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHCCCCC
HSLYSEAHVTFEEDAGAYDQTDAAGFIQLNALRLKLLAARDRRLKS
CCCHHCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA