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The map label for this gene is polA [H]

Identifier: 110677766

GI number: 110677766

Start: 360858

End: 361604

Strand: Direct

Name: polA [H]

Synonym: RD1_0367

Alternate gene names: 110677766

Gene position: 360858-361604 (Clockwise)

Preceding gene: 110677765

Following gene: 110677767

Centisome position: 8.73

GC content: 56.63

Gene sequence:

>747_bases
ATGGAAACTGGTGCTGATGCGATCACGACATGTGCCGCAGCCTTGTGCGATACGATTTACAGGGCTAACACGCAGCACAA
GGAAACGCCGAACAGGCTTTGGCGACGTCTTGCAGAAGAATATAGGAAACCCATGACCCACAACGTGTATAAGAACGATC
TGCCTGATGGGCTTGACCTCGGCCCGATGGTGGCAATTGATTGCGAGACGATGGGCCTGCACCCGCATCGCGACCGTCTG
TGCGTCGTGCAGCTTTCGGGCGGGGACGGCAAGGCGCATCTGATCCAGATCGCAAAGGGCCAGACCGAGGCGCCGAACCT
GTGCAAGCTGCTCGAAGATCCCAAGGTGCTCAAGCTGTTTCATTTCGGGCGTTTTGACATCGCGGCCATGCTCAATGCTT
TTGGCGCAACGGCGGCGCCGGTATATTGCACGAAAATCGCCAGCCGTTTGGTACGCACCTATACGGACCGCCACGGTCTG
GCCAAACTGCTGCAAGAACTCTTGGCGATCGACATTTCGAAACAGCAACAATCCAGCGACTGGGGTGCGGAGACGCTCAC
AGACGCGCAGATTGAATATGCCGCATCGGATGTTCTCTATCTGCACCAATTACGCGAGGCCCTGAACAAGATGCTGGAAC
GGGAGGGCCGCATGGAAACAGCGCAGGCCTGTTTTGACTTTCTGCCGATGCGCGCGAAACTGGACTTGATGGGGTGGCCT
GACACGGACATTTTTGCGCACTCATGA

Upstream 100 bases:

>100_bases
CTGCGCGGTCGGGTGGATCCGGAATAATGCCGGCAGTACGCGCAACATCCTTGCGAGAGGTCCAAGGGCGCGCCGCAGGG
CAACCGGTCAAGATCTGCCA

Downstream 100 bases:

>100_bases
GCGGGATATTTCAAGATACCGCGCGCCGTGTAATCCGGATCGAGATCGACGCCCTGCAACAGCTGGAGCAGAGCATCGAC
GACAGCTTTGCCAAGGCTGT

Product: 3'- 5' exonuclease family protein, putative

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
METGADAITTCAAALCDTIYRANTQHKETPNRLWRRLAEEYRKPMTHNVYKNDLPDGLDLGPMVAIDCETMGLHPHRDRL
CVVQLSGGDGKAHLIQIAKGQTEAPNLCKLLEDPKVLKLFHFGRFDIAAMLNAFGATAAPVYCTKIASRLVRTYTDRHGL
AKLLQELLAIDISKQQQSSDWGAETLTDAQIEYAASDVLYLHQLREALNKMLEREGRMETAQACFDFLPMRAKLDLMGWP
DTDIFAHS

Sequences:

>Translated_248_residues
METGADAITTCAAALCDTIYRANTQHKETPNRLWRRLAEEYRKPMTHNVYKNDLPDGLDLGPMVAIDCETMGLHPHRDRL
CVVQLSGGDGKAHLIQIAKGQTEAPNLCKLLEDPKVLKLFHFGRFDIAAMLNAFGATAAPVYCTKIASRLVRTYTDRHGL
AKLLQELLAIDISKQQQSSDWGAETLTDAQIEYAASDVLYLHQLREALNKMLEREGRMETAQACFDFLPMRAKLDLMGWP
DTDIFAHS
>Mature_248_residues
METGADAITTCAAALCDTIYRANTQHKETPNRLWRRLAEEYRKPMTHNVYKNDLPDGLDLGPMVAIDCETMGLHPHRDRL
CVVQLSGGDGKAHLIQIAKGQTEAPNLCKLLEDPKVLKLFHFGRFDIAAMLNAFGATAAPVYCTKIASRLVRTYTDRHGL
AKLLQELLAIDISKQQQSSDWGAETLTDAQIEYAASDVLYLHQLREALNKMLEREGRMETAQACFDFLPMRAKLDLMGWP
DTDIFAHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=161, Percent_Identity=31.055900621118, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 27803; Mature: 27803

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGADAITTCAAALCDTIYRANTQHKETPNRLWRRLAEEYRKPMTHNVYKNDLPDGLDL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCC
GPMVAIDCETMGLHPHRDRLCVVQLSGGDGKAHLIQIAKGQTEAPNLCKLLEDPKVLKLF
CCEEEEECHHCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHCCCHHHHHH
HFGRFDIAAMLNAFGATAAPVYCTKIASRLVRTYTDRHGLAKLLQELLAIDISKQQQSSD
HCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WGAETLTDAQIEYAASDVLYLHQLREALNKMLEREGRMETAQACFDFLPMRAKLDLMGWP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEECCCC
DTDIFAHS
CCCCCCCC
>Mature Secondary Structure
METGADAITTCAAALCDTIYRANTQHKETPNRLWRRLAEEYRKPMTHNVYKNDLPDGLDL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHCCCHHCCCCCCCCC
GPMVAIDCETMGLHPHRDRLCVVQLSGGDGKAHLIQIAKGQTEAPNLCKLLEDPKVLKLF
CCEEEEECHHCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHCCCHHHHHH
HFGRFDIAAMLNAFGATAAPVYCTKIASRLVRTYTDRHGLAKLLQELLAIDISKQQQSSD
HCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WGAETLTDAQIEYAASDVLYLHQLREALNKMLEREGRMETAQACFDFLPMRAKLDLMGWP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEECCCC
DTDIFAHS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]