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The map label for this gene is kipI [H]

Identifier: 110677649

GI number: 110677649

Start: 242542

End: 243276

Strand: Direct

Name: kipI [H]

Synonym: RD1_0243

Alternate gene names: 110677649

Gene position: 242542-243276 (Clockwise)

Preceding gene: 110677648

Following gene: 110677650

Centisome position: 5.87

GC content: 62.18

Gene sequence:

>735_bases
ATGTCCTATCCACAGATCAAGACCGTTGGCCTGTCCGGGTTACTGGTGACTTTCGCCGAAAAGATGAGCAGCCCGGCAAA
CCGTGCAGCCCTCGCATTTCGCGCCGCCGTCGTTGCCGCAGACTGGCCGGAGGTGCGCGAGACCAGCATGTCGCTCGTGT
CGGTCTATGTGGTTGCGGATCTGGTGAGCGAAGACATCCCAAAACTGATCACCAAGCTCGAAAACCTGCTGTCCCAGACC
GATTGGTACGCAGCACCCCTGCCCGAGGGGCGCAGGCTCTGGCATGTGCCGACCGTCTATGGCACCGACCTCGCCCCCCA
GCTGGAGGAGGCCGCAGAGCTTGCAGGCGTTGATCCCGACACCGCCATTCAGCAGCTTTCGACGACCCGCATGCGCGTTT
TGACAATCGGCTTTGCCCCGGGACAGCCTTATATGGGCGAGTTGGGCGAAAACTGGAACATCCCACGTCAGCAGGACCTG
ACCAAAAGCGTGCCAGCGGGGGCACTTGTGGCGGCCATCCGGCAGCTGGTCGTTTTCACGGCCCCCACCCCAACAGGCTG
GCGACACGTGGGGCAAACCGCGTTCGAGAACTTTCGCCAGACCGCCGATCAGCCCTTTGCCCTGACCCCCGGCGATGAGC
TTCTGTTTCCGCAGGTCTCGCGGCAAGCTCTGATGGACATCATGAAATCCGACACATCCGGCAATGGTGGTGCCGAGCGG
GAGACACTGCCGTGA

Upstream 100 bases:

>100_bases
GTCATGGCCCCCTTGATGGTGTTCATCGGCTGCTGGCAATTGCCCATGTACATGACGCCGATCCTGTTGCGCTGGCTCTA
CAAGCCGGAAGGGTGAGATG

Downstream 100 bases:

>100_bases
GCGGACATCTCACCATTCATGCCGCCGGTCCAGGATCATCGGTACAGGACATGGGGCGGGCGGGTTTTCTGGCCTATGGT
CTGACGCGCGGCGGGGCGGC

Product: allophanate hydrolase subunit 1, putative

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSYPQIKTVGLSGLLVTFAEKMSSPANRAALAFRAAVVAADWPEVRETSMSLVSVYVVADLVSEDIPKLITKLENLLSQT
DWYAAPLPEGRRLWHVPTVYGTDLAPQLEEAAELAGVDPDTAIQQLSTTRMRVLTIGFAPGQPYMGELGENWNIPRQQDL
TKSVPAGALVAAIRQLVVFTAPTPTGWRHVGQTAFENFRQTADQPFALTPGDELLFPQVSRQALMDIMKSDTSGNGGAER
ETLP

Sequences:

>Translated_244_residues
MSYPQIKTVGLSGLLVTFAEKMSSPANRAALAFRAAVVAADWPEVRETSMSLVSVYVVADLVSEDIPKLITKLENLLSQT
DWYAAPLPEGRRLWHVPTVYGTDLAPQLEEAAELAGVDPDTAIQQLSTTRMRVLTIGFAPGQPYMGELGENWNIPRQQDL
TKSVPAGALVAAIRQLVVFTAPTPTGWRHVGQTAFENFRQTADQPFALTPGDELLFPQVSRQALMDIMKSDTSGNGGAER
ETLP
>Mature_243_residues
SYPQIKTVGLSGLLVTFAEKMSSPANRAALAFRAAVVAADWPEVRETSMSLVSVYVVADLVSEDIPKLITKLENLLSQTD
WYAAPLPEGRRLWHVPTVYGTDLAPQLEEAAELAGVDPDTAIQQLSTTRMRVLTIGFAPGQPYMGELGENWNIPRQQDLT
KSVPAGALVAAIRQLVVFTAPTPTGWRHVGQTAFENFRQTADQPFALTPGDELLFPQVSRQALMDIMKSDTSGNGGAERE
TLP

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=125, Percent_Identity=33.6, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26551; Mature: 26419

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYPQIKTVGLSGLLVTFAEKMSSPANRAALAFRAAVVAADWPEVRETSMSLVSVYVVAD
CCCCCEEHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LVSEDIPKLITKLENLLSQTDWYAAPLPEGRRLWHVPTVYGTDLAPQLEEAAELAGVDPD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHH
TAIQQLSTTRMRVLTIGFAPGQPYMGELGENWNIPRQQDLTKSVPAGALVAAIRQLVVFT
HHHHHHHHHHEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHEEE
APTPTGWRHVGQTAFENFRQTADQPFALTPGDELLFPQVSRQALMDIMKSDTSGNGGAER
CCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHCHHHHHHHHHHHHHCCCCCCCCCCC
ETLP
CCCC
>Mature Secondary Structure 
SYPQIKTVGLSGLLVTFAEKMSSPANRAALAFRAAVVAADWPEVRETSMSLVSVYVVAD
CCCCEEHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LVSEDIPKLITKLENLLSQTDWYAAPLPEGRRLWHVPTVYGTDLAPQLEEAAELAGVDPD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHH
TAIQQLSTTRMRVLTIGFAPGQPYMGELGENWNIPRQQDLTKSVPAGALVAAIRQLVVFT
HHHHHHHHHHEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHEEE
APTPTGWRHVGQTAFENFRQTADQPFALTPGDELLFPQVSRQALMDIMKSDTSGNGGAER
CCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHCHHHHHHHHHHHHHCCCCCCCCCCC
ETLP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]