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The map label for this gene is leuD

Identifier: 110677634

GI number: 110677634

Start: 225804

End: 226409

Strand: Direct

Name: leuD

Synonym: RD1_0226

Alternate gene names: 110677634

Gene position: 225804-226409 (Clockwise)

Preceding gene: 110677633

Following gene: 110677635

Centisome position: 5.46

GC content: 54.95

Gene sequence:

>606_bases
ATGGACAAGTTCACAACACTCACCGGAATCGCGGCACCGCTGCCGCTCATCAACGTCGACACGGACATGATCATCCCAAA
GCAGTTTCTCAAAACCATCAAACGCTCGGGGCTTGGTGTCAATCTGTTCGACGAAATGCGCTATGATGACGACGGCAACG
AGATCCCCGATTTCGTCCTGAACCAAGATGCGTACCGGAAGGCAGAGATCATCGTCGCCGGGGATAACTTCGGCTGCGGG
TCCAGCCGCGAACATGCGCCTTGGGCGATCAAGGATTTTGGCATCCGCTGCGTGATTGCCCCGTCTTTTGCGGATATCTT
CTATAACAACTGCTTTAAAAACGGCATCCTGCCCATTGCCCTGCCGCAAGAGCAGGTCGATGTTCTGATGAAGGATGCCG
AAAAAGGTGCGAATGCGCGGATGACCGTGGACCTTGAGACGCAGACCGTCACCACGTCTGACGGTGAGGTGTTCAGCTTT
GATCTGGACCCGTTCAAAAAGCGCTGCCTGATGGAAGGTCTCGACGATATCGGTCTGACGATGGAAAAGATCAGCGCGAT
CGACAGTTTTGAGCAAAGCGCGGCACAAGCCCGCCCTTGGGTCTGA

Upstream 100 bases:

>100_bases
GGTGATTGCAAACTGTGGTGAAATACGGCATCACAGCGCCAAACGAATGGCCTAATCAGCGCCAACGACCCAAGGCCCCC
TGCGGAGCGAGGAAAAACAG

Downstream 100 bases:

>100_bases
TCACCACGTAATATCGTCCATGCGTTAACCACTGTTAAAGGTTCTTGGCCTTTATGGTTAACGATATGATGTTTCTGCGG
CATGCTGCCCGTTTGCGTTC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MDKFTTLTGIAAPLPLINVDTDMIIPKQFLKTIKRSGLGVNLFDEMRYDDDGNEIPDFVLNQDAYRKAEIIVAGDNFGCG
SSREHAPWAIKDFGIRCVIAPSFADIFYNNCFKNGILPIALPQEQVDVLMKDAEKGANARMTVDLETQTVTTSDGEVFSF
DLDPFKKRCLMEGLDDIGLTMEKISAIDSFEQSAAQARPWV

Sequences:

>Translated_201_residues
MDKFTTLTGIAAPLPLINVDTDMIIPKQFLKTIKRSGLGVNLFDEMRYDDDGNEIPDFVLNQDAYRKAEIIVAGDNFGCG
SSREHAPWAIKDFGIRCVIAPSFADIFYNNCFKNGILPIALPQEQVDVLMKDAEKGANARMTVDLETQTVTTSDGEVFSF
DLDPFKKRCLMEGLDDIGLTMEKISAIDSFEQSAAQARPWV
>Mature_201_residues
MDKFTTLTGIAAPLPLINVDTDMIIPKQFLKTIKRSGLGVNLFDEMRYDDDGNEIPDFVLNQDAYRKAEIIVAGDNFGCG
SSREHAPWAIKDFGIRCVIAPSFADIFYNNCFKNGILPIALPQEQVDVLMKDAEKGANARMTVDLETQTVTTSDGEVFSF
DLDPFKKRCLMEGLDDIGLTMEKISAIDSFEQSAAQARPWV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=192, Percent_Identity=47.9166666666667, Blast_Score=191, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=57.5757575757576, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_ROSDO (Q16DI8)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_680641.1
- ProteinModelPortal:   Q16DI8
- SMR:   Q16DI8
- STRING:   Q16DI8
- GeneID:   4198176
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_0226
- NMPDR:   fig|375451.6.peg.213
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q16DI8
- ProtClustDB:   PRK01641
- BioCyc:   RDEN375451:RD1_0226-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22339; Mature: 22339

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFTTLTGIAAPLPLINVDTDMIIPKQFLKTIKRSGLGVNLFDEMRYDDDGNEIPDFVL
CCCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCHHHC
NQDAYRKAEIIVAGDNFGCGSSREHAPWAIKDFGIRCVIAPSFADIFYNNCFKNGILPIA
CCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHCCCEEEE
LPQEQVDVLMKDAEKGANARMTVDLETQTVTTSDGEVFSFDLDPFKKRCLMEGLDDIGLT
CCHHHHHHHHHHHHCCCCCEEEEEEECEEEECCCCCEEEECCCHHHHHHHHHCCHHHCHH
MEKISAIDSFEQSAAQARPWV
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKFTTLTGIAAPLPLINVDTDMIIPKQFLKTIKRSGLGVNLFDEMRYDDDGNEIPDFVL
CCCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCHHHC
NQDAYRKAEIIVAGDNFGCGSSREHAPWAIKDFGIRCVIAPSFADIFYNNCFKNGILPIA
CCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHCCCEEEE
LPQEQVDVLMKDAEKGANARMTVDLETQTVTTSDGEVFSFDLDPFKKRCLMEGLDDIGLT
CCHHHHHHHHHHHHCCCCCEEEEEEECEEEECCCCCEEEECCCHHHHHHHHHCCHHHCHH
MEKISAIDSFEQSAAQARPWV
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA